Leuconostoc fallax
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1619 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R5N8V2|A0A4R5N8V2_9LACO DUF2179 domain-containing protein OS=Leuconostoc fallax OX=1251 GN=C5L23_000613 PE=4 SV=1
MM1 pKa = 7.22 TAINDD6 pKa = 3.89 ANSMLNAIEE15 pKa = 4.89 SGDD18 pKa = 3.69 LVSAQNYY25 pKa = 7.38 FKK27 pKa = 11.04 KK28 pKa = 10.59 SLQNDD33 pKa = 3.61 SDD35 pKa = 3.74 EE36 pKa = 5.41 LIYY39 pKa = 11.21 NLAEE43 pKa = 3.96 EE44 pKa = 4.89 LYY46 pKa = 11.28 GMGFTHH52 pKa = 6.34 YY53 pKa = 10.68 AKK55 pKa = 10.32 EE56 pKa = 4.09 AYY58 pKa = 9.0 EE59 pKa = 4.19 LLLTRR64 pKa = 11.84 YY65 pKa = 8.26 PQEE68 pKa = 4.05 DD69 pKa = 4.11 SIRR72 pKa = 11.84 TALADD77 pKa = 3.32 IAIDD81 pKa = 4.62 DD82 pKa = 4.43 GEE84 pKa = 4.56 IDD86 pKa = 3.58 HH87 pKa = 7.05 ALEE90 pKa = 5.06 LLSQVNPEE98 pKa = 3.39 SDD100 pKa = 3.75 AYY102 pKa = 11.14 VSSLLVKK109 pKa = 10.49 ADD111 pKa = 4.53 LYY113 pKa = 11.12 QSEE116 pKa = 4.64 GLNEE120 pKa = 3.93 AAEE123 pKa = 4.3 SSLRR127 pKa = 11.84 RR128 pKa = 11.84 AQQLMPDD135 pKa = 3.4 HH136 pKa = 7.55 DD137 pKa = 5.17 IIQLALAEE145 pKa = 4.84 FYY147 pKa = 11.13 FDD149 pKa = 2.9 IQSYY153 pKa = 8.76 HH154 pKa = 4.75 QAIPIYY160 pKa = 10.06 RR161 pKa = 11.84 RR162 pKa = 11.84 LIMQGTLEE170 pKa = 4.01 VSKK173 pKa = 10.72 IDD175 pKa = 3.12 IVARR179 pKa = 11.84 LGVAYY184 pKa = 9.88 AQIGNYY190 pKa = 10.11 DD191 pKa = 3.39 NAIGYY196 pKa = 9.29 LEE198 pKa = 4.06 QIKK201 pKa = 10.4 PEE203 pKa = 4.51 NITLTTQFQLAVVYY217 pKa = 8.09 YY218 pKa = 10.77 QNTQWQDD225 pKa = 3.33 AIDD228 pKa = 4.49 LFNDD232 pKa = 4.64 IIDD235 pKa = 3.84 VDD237 pKa = 3.87 PKK239 pKa = 9.18 YY240 pKa = 9.54 TSVYY244 pKa = 9.25 PLLGKK249 pKa = 10.3 AYY251 pKa = 9.31 EE252 pKa = 4.24 KK253 pKa = 10.93 LNKK256 pKa = 9.03 IDD258 pKa = 3.35 EE259 pKa = 4.75 AYY261 pKa = 10.43 RR262 pKa = 11.84 IYY264 pKa = 10.67 QEE266 pKa = 5.15 GLSQDD271 pKa = 3.51 EE272 pKa = 4.85 TNSQLYY278 pKa = 9.63 RR279 pKa = 11.84 LAGITAQKK287 pKa = 10.69 LNEE290 pKa = 4.24 LEE292 pKa = 4.11 SAEE295 pKa = 4.33 TYY297 pKa = 9.79 LVKK300 pKa = 10.8 AIALDD305 pKa = 3.96 EE306 pKa = 4.89 ADD308 pKa = 3.47 TMAITSLAQVLLLQDD323 pKa = 3.67 RR324 pKa = 11.84 AAEE327 pKa = 4.34 VITLILDD334 pKa = 3.45 QQEE337 pKa = 3.99 NDD339 pKa = 3.65 VIDD342 pKa = 3.78 NSFYY346 pKa = 10.15 WVLAKK351 pKa = 10.33 AYY353 pKa = 9.75 EE354 pKa = 4.13 MTDD357 pKa = 3.35 EE358 pKa = 4.23 YY359 pKa = 11.4 EE360 pKa = 4.34 KK361 pKa = 11.14 ASEE364 pKa = 4.37 NYY366 pKa = 10.06 DD367 pKa = 3.85 LAWPLLQNNTDD378 pKa = 3.9 FLLDD382 pKa = 3.3 AMAWYY387 pKa = 10.17 RR388 pKa = 11.84 EE389 pKa = 3.85 NGQRR393 pKa = 11.84 DD394 pKa = 3.52 IEE396 pKa = 4.28 IQSLQQYY403 pKa = 9.93 LKK405 pKa = 10.71 LVPDD409 pKa = 5.26 DD410 pKa = 5.02 IEE412 pKa = 4.24 MQLRR416 pKa = 11.84 LADD419 pKa = 3.95 YY420 pKa = 10.58 EE421 pKa = 4.16 NDD423 pKa = 3.51 SMM425 pKa = 5.97
Molecular weight: 48.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 4.012
IPC_protein 4.012
Toseland 3.808
ProMoST 4.139
Dawson 3.986
Bjellqvist 4.126
Wikipedia 3.884
Rodwell 3.834
Grimsley 3.719
Solomon 3.973
Lehninger 3.935
Nozaki 4.088
DTASelect 4.291
Thurlkill 3.834
EMBOSS 3.897
Sillero 4.126
Patrickios 1.456
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.005
Protein with the highest isoelectric point:
>tr|A0A4V3A2H1|A0A4V3A2H1_9LACO Uncharacterized protein OS=Leuconostoc fallax OX=1251 GN=C5L23_000634 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.34 KK9 pKa = 7.47 RR10 pKa = 11.84 HH11 pKa = 5.71 RR12 pKa = 11.84 EE13 pKa = 3.7 RR14 pKa = 11.84 VHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.23 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.193
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.93
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1619
0
1619
490441
30
1901
302.9
33.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.157 ± 0.07
0.265 ± 0.025
5.821 ± 0.059
5.235 ± 0.061
4.265 ± 0.051
6.386 ± 0.053
2.272 ± 0.029
7.735 ± 0.065
5.855 ± 0.059
9.9 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.674 ± 0.03
4.962 ± 0.046
3.399 ± 0.029
5.229 ± 0.06
4.008 ± 0.049
6.027 ± 0.072
6.208 ± 0.049
7.056 ± 0.049
1.049 ± 0.026
3.472 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here