Cystobacter fuscus DSM 2262
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10511 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S9QTE0|S9QTE0_9DELT Uncharacterized protein OS=Cystobacter fuscus DSM 2262 OX=1242864 GN=D187_002672 PE=4 SV=1
MM1 pKa = 7.15 GWALALALMGAQLGCGSDD19 pKa = 3.42 SDD21 pKa = 4.58 GDD23 pKa = 4.42 GIHH26 pKa = 7.54 DD27 pKa = 4.79 GADD30 pKa = 3.43 CAPDD34 pKa = 3.97 SDD36 pKa = 5.19 SSWKK40 pKa = 9.73 QLTMYY45 pKa = 10.95 DD46 pKa = 3.98 DD47 pKa = 4.89 ADD49 pKa = 3.65 GDD51 pKa = 4.17 KK52 pKa = 10.84 YY53 pKa = 11.37 GSGEE57 pKa = 3.85 PRR59 pKa = 11.84 MICIGKK65 pKa = 7.26 TLPPGLSTQGGDD77 pKa = 4.32 CAPEE81 pKa = 4.27 DD82 pKa = 3.54 ATRR85 pKa = 11.84 WMSLTGKK92 pKa = 10.25 GYY94 pKa = 10.68 YY95 pKa = 10.22 ADD97 pKa = 4.42 ADD99 pKa = 3.95 GDD101 pKa = 4.1 GKK103 pKa = 10.77 AVHH106 pKa = 6.75 APSSDD111 pKa = 3.35 CVGANTTGYY120 pKa = 10.77 LRR122 pKa = 11.84 VQGTDD127 pKa = 4.11 CDD129 pKa = 4.18 DD130 pKa = 4.74 ADD132 pKa = 3.88 PKK134 pKa = 11.09 VWTSRR139 pKa = 11.84 SLYY142 pKa = 10.58 ADD144 pKa = 3.48 EE145 pKa = 6.35 DD146 pKa = 4.51 GDD148 pKa = 4.23 GVAEE152 pKa = 4.63 GEE154 pKa = 4.47 ASLHH158 pKa = 6.49 CIGSSVPPGMSEE170 pKa = 4.06 TASDD174 pKa = 4.31 CAPGDD179 pKa = 3.63 STRR182 pKa = 11.84 YY183 pKa = 7.96 RR184 pKa = 11.84 TWNHH188 pKa = 5.15 VGRR191 pKa = 11.84 DD192 pKa = 3.37 EE193 pKa = 5.73 DD194 pKa = 4.16 GDD196 pKa = 4.16 GFFVSAPGTLCIGEE210 pKa = 4.7 APPAGYY216 pKa = 8.67 TSSTLTRR223 pKa = 11.84 LDD225 pKa = 4.03 CDD227 pKa = 3.63 DD228 pKa = 3.71 TRR230 pKa = 11.84 ADD232 pKa = 2.83 VWTPLVQYY240 pKa = 10.42 LDD242 pKa = 3.6 SDD244 pKa = 4.05 GDD246 pKa = 3.94 QVGSGPAEE254 pKa = 4.06 TLCSGEE260 pKa = 4.09 GVRR263 pKa = 11.84 PGYY266 pKa = 10.55 SLGAGDD272 pKa = 5.64 CAPDD276 pKa = 4.62 DD277 pKa = 3.7 NTRR280 pKa = 11.84 WMLHH284 pKa = 5.38 SYY286 pKa = 9.81 LWRR289 pKa = 11.84 DD290 pKa = 2.96 ADD292 pKa = 3.84 GDD294 pKa = 4.19 GATVSQTGQVCGGFQIPAGFSMTPGTHH321 pKa = 7.05 LDD323 pKa = 3.75 CDD325 pKa = 3.71 DD326 pKa = 3.55 TRR328 pKa = 11.84 AEE330 pKa = 4.32 VSVTWSVFPDD340 pKa = 3.42 ADD342 pKa = 3.47 QDD344 pKa = 4.17 GVGAGAQRR352 pKa = 11.84 TICAGTTRR360 pKa = 11.84 PAGYY364 pKa = 10.4 ADD366 pKa = 4.19 TGTDD370 pKa = 3.87 CAPDD374 pKa = 3.62 DD375 pKa = 3.92 ALRR378 pKa = 11.84 WRR380 pKa = 11.84 TFTPRR385 pKa = 11.84 FRR387 pKa = 11.84 DD388 pKa = 3.25 TDD390 pKa = 3.51 GDD392 pKa = 4.21 GFTVAPGGPVCLGASTQGYY411 pKa = 8.43 PDD413 pKa = 3.47 TSRR416 pKa = 11.84 GTDD419 pKa = 3.33 CDD421 pKa = 3.53 DD422 pKa = 3.4 TRR424 pKa = 11.84 AEE426 pKa = 4.67 VYY428 pKa = 10.3 QSLQVHH434 pKa = 5.98 VDD436 pKa = 3.09 MDD438 pKa = 4.32 GDD440 pKa = 4.51 GVGSGPALTLCTNGSPPAGHH460 pKa = 6.58 SLQGTDD466 pKa = 4.64 CAPDD470 pKa = 3.71 DD471 pKa = 3.92 ASRR474 pKa = 11.84 WRR476 pKa = 11.84 LHH478 pKa = 5.29 AFKK481 pKa = 10.92 YY482 pKa = 10.6 VDD484 pKa = 3.79 ADD486 pKa = 3.87 GDD488 pKa = 4.14 GHH490 pKa = 5.95 TLPSTGQVCAAATLPPPYY508 pKa = 9.68 TPSASGNDD516 pKa = 4.03 CDD518 pKa = 5.47 DD519 pKa = 4.51 ADD521 pKa = 3.88 PARR524 pKa = 11.84 FLWRR528 pKa = 11.84 VLYY531 pKa = 9.75 PDD533 pKa = 4.0 RR534 pKa = 11.84 DD535 pKa = 3.57 GDD537 pKa = 4.54 GVGTPPRR544 pKa = 11.84 VVLCLDD550 pKa = 4.47 DD551 pKa = 5.49 APPPPGYY558 pKa = 10.05 SIYY561 pKa = 10.97 GFDD564 pKa = 5.41 PDD566 pKa = 5.36 DD567 pKa = 4.65 SDD569 pKa = 4.0 PASRR573 pKa = 11.84 DD574 pKa = 3.36 PADD577 pKa = 3.69 EE578 pKa = 4.64 SEE580 pKa = 4.24 LDD582 pKa = 3.54 EE583 pKa = 5.97 FLFTQQ588 pKa = 4.37
Molecular weight: 61.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.795
IPC_protein 3.859
Toseland 3.617
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.681
Grimsley 3.516
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.317
Thurlkill 3.681
EMBOSS 3.859
Sillero 3.986
Patrickios 0.884
IPC_peptide 3.872
IPC2_peptide 3.961
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|S9PCR5|S9PCR5_9DELT ATEPR1 protein OS=Cystobacter fuscus DSM 2262 OX=1242864 GN=D187_001548 PE=4 SV=1
MM1 pKa = 7.85 PSRR4 pKa = 11.84 TRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 GGGWTWRR15 pKa = 11.84 SAARR19 pKa = 11.84 ARR21 pKa = 11.84 GPVTHH26 pKa = 6.94 ARR28 pKa = 11.84 ASAPGALAPGGRR40 pKa = 11.84 RR41 pKa = 11.84 AA42 pKa = 3.86
Molecular weight: 4.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.513
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.457
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.193
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.197
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10511
0
10511
3636354
37
8515
346.0
37.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.331 ± 0.034
0.948 ± 0.009
4.861 ± 0.015
6.632 ± 0.029
3.361 ± 0.015
8.714 ± 0.025
2.253 ± 0.012
3.054 ± 0.019
2.816 ± 0.022
11.228 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.962 ± 0.01
2.273 ± 0.019
6.111 ± 0.026
3.5 ± 0.015
8.098 ± 0.029
5.963 ± 0.021
5.416 ± 0.022
7.793 ± 0.019
1.487 ± 0.01
2.201 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here