Cystobacter fuscus DSM 2262

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Cystobacterineae; Archangiaceae; Cystobacter; Cystobacter fuscus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10511 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S9QTE0|S9QTE0_9DELT Uncharacterized protein OS=Cystobacter fuscus DSM 2262 OX=1242864 GN=D187_002672 PE=4 SV=1
MM1 pKa = 7.15GWALALALMGAQLGCGSDD19 pKa = 3.42SDD21 pKa = 4.58GDD23 pKa = 4.42GIHH26 pKa = 7.54DD27 pKa = 4.79GADD30 pKa = 3.43CAPDD34 pKa = 3.97SDD36 pKa = 5.19SSWKK40 pKa = 9.73QLTMYY45 pKa = 10.95DD46 pKa = 3.98DD47 pKa = 4.89ADD49 pKa = 3.65GDD51 pKa = 4.17KK52 pKa = 10.84YY53 pKa = 11.37GSGEE57 pKa = 3.85PRR59 pKa = 11.84MICIGKK65 pKa = 7.26TLPPGLSTQGGDD77 pKa = 4.32CAPEE81 pKa = 4.27DD82 pKa = 3.54ATRR85 pKa = 11.84WMSLTGKK92 pKa = 10.25GYY94 pKa = 10.68YY95 pKa = 10.22ADD97 pKa = 4.42ADD99 pKa = 3.95GDD101 pKa = 4.1GKK103 pKa = 10.77AVHH106 pKa = 6.75APSSDD111 pKa = 3.35CVGANTTGYY120 pKa = 10.77LRR122 pKa = 11.84VQGTDD127 pKa = 4.11CDD129 pKa = 4.18DD130 pKa = 4.74ADD132 pKa = 3.88PKK134 pKa = 11.09VWTSRR139 pKa = 11.84SLYY142 pKa = 10.58ADD144 pKa = 3.48EE145 pKa = 6.35DD146 pKa = 4.51GDD148 pKa = 4.23GVAEE152 pKa = 4.63GEE154 pKa = 4.47ASLHH158 pKa = 6.49CIGSSVPPGMSEE170 pKa = 4.06TASDD174 pKa = 4.31CAPGDD179 pKa = 3.63STRR182 pKa = 11.84YY183 pKa = 7.96RR184 pKa = 11.84TWNHH188 pKa = 5.15VGRR191 pKa = 11.84DD192 pKa = 3.37EE193 pKa = 5.73DD194 pKa = 4.16GDD196 pKa = 4.16GFFVSAPGTLCIGEE210 pKa = 4.7APPAGYY216 pKa = 8.67TSSTLTRR223 pKa = 11.84LDD225 pKa = 4.03CDD227 pKa = 3.63DD228 pKa = 3.71TRR230 pKa = 11.84ADD232 pKa = 2.83VWTPLVQYY240 pKa = 10.42LDD242 pKa = 3.6SDD244 pKa = 4.05GDD246 pKa = 3.94QVGSGPAEE254 pKa = 4.06TLCSGEE260 pKa = 4.09GVRR263 pKa = 11.84PGYY266 pKa = 10.55SLGAGDD272 pKa = 5.64CAPDD276 pKa = 4.62DD277 pKa = 3.7NTRR280 pKa = 11.84WMLHH284 pKa = 5.38SYY286 pKa = 9.81LWRR289 pKa = 11.84DD290 pKa = 2.96ADD292 pKa = 3.84GDD294 pKa = 4.19GATVSQTGQVCGGFQIPAGFSMTPGTHH321 pKa = 7.05LDD323 pKa = 3.75CDD325 pKa = 3.71DD326 pKa = 3.55TRR328 pKa = 11.84AEE330 pKa = 4.32VSVTWSVFPDD340 pKa = 3.42ADD342 pKa = 3.47QDD344 pKa = 4.17GVGAGAQRR352 pKa = 11.84TICAGTTRR360 pKa = 11.84PAGYY364 pKa = 10.4ADD366 pKa = 4.19TGTDD370 pKa = 3.87CAPDD374 pKa = 3.62DD375 pKa = 3.92ALRR378 pKa = 11.84WRR380 pKa = 11.84TFTPRR385 pKa = 11.84FRR387 pKa = 11.84DD388 pKa = 3.25TDD390 pKa = 3.51GDD392 pKa = 4.21GFTVAPGGPVCLGASTQGYY411 pKa = 8.43PDD413 pKa = 3.47TSRR416 pKa = 11.84GTDD419 pKa = 3.33CDD421 pKa = 3.53DD422 pKa = 3.4TRR424 pKa = 11.84AEE426 pKa = 4.67VYY428 pKa = 10.3QSLQVHH434 pKa = 5.98VDD436 pKa = 3.09MDD438 pKa = 4.32GDD440 pKa = 4.51GVGSGPALTLCTNGSPPAGHH460 pKa = 6.58SLQGTDD466 pKa = 4.64CAPDD470 pKa = 3.71DD471 pKa = 3.92ASRR474 pKa = 11.84WRR476 pKa = 11.84LHH478 pKa = 5.29AFKK481 pKa = 10.92YY482 pKa = 10.6VDD484 pKa = 3.79ADD486 pKa = 3.87GDD488 pKa = 4.14GHH490 pKa = 5.95TLPSTGQVCAAATLPPPYY508 pKa = 9.68TPSASGNDD516 pKa = 4.03CDD518 pKa = 5.47DD519 pKa = 4.51ADD521 pKa = 3.88PARR524 pKa = 11.84FLWRR528 pKa = 11.84VLYY531 pKa = 9.75PDD533 pKa = 4.0RR534 pKa = 11.84DD535 pKa = 3.57GDD537 pKa = 4.54GVGTPPRR544 pKa = 11.84VVLCLDD550 pKa = 4.47DD551 pKa = 5.49APPPPGYY558 pKa = 10.05SIYY561 pKa = 10.97GFDD564 pKa = 5.41PDD566 pKa = 5.36DD567 pKa = 4.65SDD569 pKa = 4.0PASRR573 pKa = 11.84DD574 pKa = 3.36PADD577 pKa = 3.69EE578 pKa = 4.64SEE580 pKa = 4.24LDD582 pKa = 3.54EE583 pKa = 5.97FLFTQQ588 pKa = 4.37

Molecular weight:
61.36 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S9PCR5|S9PCR5_9DELT ATEPR1 protein OS=Cystobacter fuscus DSM 2262 OX=1242864 GN=D187_001548 PE=4 SV=1
MM1 pKa = 7.85PSRR4 pKa = 11.84TRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84GGGWTWRR15 pKa = 11.84SAARR19 pKa = 11.84ARR21 pKa = 11.84GPVTHH26 pKa = 6.94ARR28 pKa = 11.84ASAPGALAPGGRR40 pKa = 11.84RR41 pKa = 11.84AA42 pKa = 3.86

Molecular weight:
4.43 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10511

0

10511

3636354

37

8515

346.0

37.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.331 ± 0.034

0.948 ± 0.009

4.861 ± 0.015

6.632 ± 0.029

3.361 ± 0.015

8.714 ± 0.025

2.253 ± 0.012

3.054 ± 0.019

2.816 ± 0.022

11.228 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.962 ± 0.01

2.273 ± 0.019

6.111 ± 0.026

3.5 ± 0.015

8.098 ± 0.029

5.963 ± 0.021

5.416 ± 0.022

7.793 ± 0.019

1.487 ± 0.01

2.201 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski