Lactobacillus apis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1508 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4LS12|A0A0F4LS12_9LACO 3-hydroxy-3-methylglutaryl coenzyme A reductase OS=Lactobacillus apis OX=303541 GN=JF72_04000 PE=3 SV=1
MM1 pKa = 7.73KK2 pKa = 10.46ARR4 pKa = 11.84IVYY7 pKa = 10.79ASMTGNDD14 pKa = 3.85ADD16 pKa = 3.73MADD19 pKa = 3.71ILEE22 pKa = 5.0EE23 pKa = 4.3DD24 pKa = 3.63LQDD27 pKa = 3.43YY28 pKa = 10.42GFDD31 pKa = 3.74VEE33 pKa = 4.34TSEE36 pKa = 6.36AEE38 pKa = 3.92FTDD41 pKa = 3.58ASDD44 pKa = 3.75YY45 pKa = 9.65LTSDD49 pKa = 3.38LCIFVTYY56 pKa = 9.54TYY58 pKa = 11.36GEE60 pKa = 4.15GKK62 pKa = 7.98MTDD65 pKa = 3.64DD66 pKa = 3.47VADD69 pKa = 4.74FYY71 pKa = 11.67EE72 pKa = 4.13QLKK75 pKa = 10.64EE76 pKa = 3.76LDD78 pKa = 3.47LSGKK82 pKa = 9.29YY83 pKa = 8.95FAVMGSGDD91 pKa = 3.96MVYY94 pKa = 11.08ADD96 pKa = 4.42HH97 pKa = 7.01FCEE100 pKa = 4.56NVFDD104 pKa = 4.28FEE106 pKa = 5.61KK107 pKa = 10.49MFNQVGAKK115 pKa = 9.37EE116 pKa = 4.07VTKK119 pKa = 10.48PVTIEE124 pKa = 3.91NAPDD128 pKa = 4.54DD129 pKa = 4.96DD130 pKa = 6.65DD131 pKa = 5.01IAAIDD136 pKa = 3.73NAAKK140 pKa = 10.43EE141 pKa = 4.23MADD144 pKa = 3.42QLNGG148 pKa = 3.39

Molecular weight: 16.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4LVV7|A0A0F4LVV7_9LACO Uncharacterized protein OS=Lactobacillus apis OX=303541 GN=JF72_09600 PE=4 SV=1
MM1 pKa = 7.87PKK3 pKa = 9.59MKK5 pKa = 8.8THH7 pKa = 6.78RR8 pKa = 11.84ASAKK12 pKa = 8.58RR13 pKa = 11.84FKK15 pKa = 10.27RR16 pKa = 11.84TGSGQLKK23 pKa = 8.99RR24 pKa = 11.84HH25 pKa = 5.86HH26 pKa = 7.18AFTGHH31 pKa = 6.77RR32 pKa = 11.84FHH34 pKa = 7.54GKK36 pKa = 6.24TKK38 pKa = 9.47KK39 pKa = 9.37QRR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.62LRR45 pKa = 11.84KK46 pKa = 8.85AALVSRR52 pKa = 11.84SDD54 pKa = 3.6LKK56 pKa = 10.83RR57 pKa = 11.84IKK59 pKa = 10.66QMLSQMRR66 pKa = 3.59

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1508

0

1508

487674

38

3395

323.4

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.299 ± 0.066

0.606 ± 0.016

6.032 ± 0.072

6.067 ± 0.066

4.288 ± 0.057

6.328 ± 0.055

1.915 ± 0.025

7.496 ± 0.063

7.751 ± 0.062

9.43 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.037

5.585 ± 0.067

3.455 ± 0.033

4.173 ± 0.047

3.667 ± 0.045

6.217 ± 0.056

5.709 ± 0.041

6.825 ± 0.054

0.906 ± 0.022

3.76 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski