Bos taurus papillomavirus 19
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B2K234|A0A1B2K234_9PAPI Regulatory protein E2 OS=Bos taurus papillomavirus 19 OX=1887217 GN=E2 PE=3 SV=1
MM1 pKa = 7.08 TLRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 9.59 RR8 pKa = 11.84 DD9 pKa = 3.18 SAEE12 pKa = 3.83 NLWRR16 pKa = 11.84 HH17 pKa = 5.31 CQATGAEE24 pKa = 4.51 CPPDD28 pKa = 4.03 VINKK32 pKa = 6.84 YY33 pKa = 8.0 TEE35 pKa = 3.6 NTLADD40 pKa = 3.99 RR41 pKa = 11.84 LSKK44 pKa = 10.5 IFASILYY51 pKa = 10.15 LGGLAIGTGKK61 pKa = 10.87 GSGGNLGYY69 pKa = 10.38 RR70 pKa = 11.84 PLGEE74 pKa = 3.98 PVGPPRR80 pKa = 11.84 VGPGGTVIRR89 pKa = 11.84 PNVVVDD95 pKa = 3.85 AVGPADD101 pKa = 6.23 LIPLDD106 pKa = 4.13 SLNPDD111 pKa = 3.24 SSVIPLLRR119 pKa = 11.84 GTPEE123 pKa = 3.8 GSEE126 pKa = 4.23 VGLEE130 pKa = 4.14 TPDD133 pKa = 3.36 ILGEE137 pKa = 4.15 SDD139 pKa = 3.49 PASDD143 pKa = 3.79 VTITTGNTVTLGEE156 pKa = 4.39 DD157 pKa = 3.35 TPAILEE163 pKa = 4.23 VTPVQPEE170 pKa = 3.97 EE171 pKa = 4.36 AVPPPAKK178 pKa = 9.9 RR179 pKa = 11.84 PRR181 pKa = 11.84 VSAQEE186 pKa = 4.09 FLNATYY192 pKa = 10.46 DD193 pKa = 3.5 PSIFSTEE200 pKa = 3.96 PVTLSTTGTASNSVTVDD217 pKa = 3.97 FGLPSTEE224 pKa = 3.39 IGQVFEE230 pKa = 4.91 EE231 pKa = 4.42 IEE233 pKa = 4.12 LDD235 pKa = 3.37 EE236 pKa = 4.37 FTPLTRR242 pKa = 11.84 SPQASTPADD251 pKa = 3.32 EE252 pKa = 5.03 SYY254 pKa = 11.79 GLFSRR259 pKa = 11.84 IRR261 pKa = 11.84 EE262 pKa = 4.09 FYY264 pKa = 10.03 NRR266 pKa = 11.84 RR267 pKa = 11.84 IRR269 pKa = 11.84 QVEE272 pKa = 3.97 ITSPTFLRR280 pKa = 11.84 RR281 pKa = 11.84 PQAYY285 pKa = 8.51 IDD287 pKa = 3.55 FAYY290 pKa = 10.29 GVDD293 pKa = 4.18 NPAFDD298 pKa = 5.06 PDD300 pKa = 3.37 VSRR303 pKa = 11.84 EE304 pKa = 3.88 FLEE307 pKa = 5.4 DD308 pKa = 3.36 LAEE311 pKa = 4.37 LEE313 pKa = 4.53 NVTAAPHH320 pKa = 6.83 SDD322 pKa = 3.19 FQDD325 pKa = 2.88 IVRR328 pKa = 11.84 LNRR331 pKa = 11.84 QVFQEE336 pKa = 4.54 GEE338 pKa = 4.09 GGRR341 pKa = 11.84 LRR343 pKa = 11.84 VSRR346 pKa = 11.84 IGNRR350 pKa = 11.84 GTISTRR356 pKa = 11.84 SGLLIGEE363 pKa = 4.19 RR364 pKa = 11.84 VHH366 pKa = 7.34 FYY368 pKa = 11.06 QDD370 pKa = 3.17 LSSIQQSDD378 pKa = 4.48 GIEE381 pKa = 3.97 LAPLGSFTGEE391 pKa = 3.8 ATVVTGTEE399 pKa = 3.34 EE400 pKa = 4.17 GAFVDD405 pKa = 4.33 ASSSFDD411 pKa = 3.58 YY412 pKa = 10.81 EE413 pKa = 4.21 GTGIEE418 pKa = 5.98 DD419 pKa = 3.58 VLLDD423 pKa = 3.58 NYY425 pKa = 10.49 EE426 pKa = 4.08 EE427 pKa = 4.37 SFEE430 pKa = 4.27 RR431 pKa = 11.84 SQLVIGTRR439 pKa = 11.84 RR440 pKa = 11.84 SSNIVPMDD448 pKa = 3.89 FYY450 pKa = 10.81 RR451 pKa = 11.84 GEE453 pKa = 3.9 IRR455 pKa = 11.84 PFVQDD460 pKa = 3.37 LTEE463 pKa = 3.84 GWFYY467 pKa = 11.32 GPPEE471 pKa = 4.42 IGNDD475 pKa = 3.64 EE476 pKa = 4.11 NTPIYY481 pKa = 10.19 IDD483 pKa = 3.78 WSPLEE488 pKa = 4.35 PGSRR492 pKa = 11.84 GPDD495 pKa = 3.67 LIINLYY501 pKa = 9.85 GNDD504 pKa = 3.64 YY505 pKa = 10.49 SLHH508 pKa = 6.19 PSYY511 pKa = 11.27 LLRR514 pKa = 11.84 RR515 pKa = 11.84 RR516 pKa = 11.84 RR517 pKa = 11.84 KK518 pKa = 8.33 RR519 pKa = 11.84 KK520 pKa = 9.47 RR521 pKa = 11.84 FHH523 pKa = 6.41 VLL525 pKa = 2.81
Molecular weight: 57.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.652
IPC2_protein 4.584
IPC_protein 4.546
Toseland 4.38
ProMoST 4.635
Dawson 4.482
Bjellqvist 4.635
Wikipedia 4.342
Rodwell 4.38
Grimsley 4.291
Solomon 4.482
Lehninger 4.431
Nozaki 4.584
DTASelect 4.736
Thurlkill 4.38
EMBOSS 4.368
Sillero 4.647
Patrickios 3.999
IPC_peptide 4.482
IPC2_peptide 4.647
IPC2.peptide.svr19 4.571
Protein with the highest isoelectric point:
>tr|A0A1B2K268|A0A1B2K268_9PAPI Replication protein E1 OS=Bos taurus papillomavirus 19 OX=1887217 GN=E1 PE=3 SV=1
MM1 pKa = 7.54 KK2 pKa = 8.68 PTVIVCFFMGKK13 pKa = 8.57 EE14 pKa = 4.18 SRR16 pKa = 11.84 CMPGIFGPGMVRR28 pKa = 11.84 LEE30 pKa = 3.77 KK31 pKa = 10.34 RR32 pKa = 11.84 YY33 pKa = 9.77 LQMEE37 pKa = 4.51 KK38 pKa = 10.86 LGMTFSLVPLVALAKK53 pKa = 10.43 ALIIILLVPVGQWLPQMLTYY73 pKa = 9.9 LTGHH77 pKa = 6.81 FGCNEE82 pKa = 3.64 HH83 pKa = 6.85 KK84 pKa = 10.58 AIIMALLGEE93 pKa = 4.49 ISYY96 pKa = 10.61 LLQWLIIPATQTILL110 pKa = 3.46
Molecular weight: 12.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.587
IPC2_protein 8.375
IPC_protein 8.434
Toseland 8.77
ProMoST 8.785
Dawson 9.107
Bjellqvist 9.18
Wikipedia 9.165
Rodwell 9.209
Grimsley 8.668
Solomon 9.355
Lehninger 9.355
Nozaki 9.399
DTASelect 9.004
Thurlkill 9.092
EMBOSS 9.297
Sillero 9.341
Patrickios 4.507
IPC_peptide 9.355
IPC2_peptide 8.156
IPC2.peptide.svr19 7.856
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2383
95
612
340.4
38.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.581 ± 0.349
2.266 ± 0.696
6.085 ± 0.633
6.504 ± 0.635
4.532 ± 0.588
7.134 ± 0.873
1.888 ± 0.253
4.826 ± 0.615
5.455 ± 1.105
9.987 ± 0.964
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.804 ± 0.497
4.154 ± 0.565
5.581 ± 0.76
3.777 ± 0.306
6.085 ± 1.03
7.512 ± 0.761
6.211 ± 0.304
5.917 ± 0.361
1.553 ± 0.301
3.147 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here