Streptococcus satellite phage Javan721
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZW49|A0A4D5ZW49_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan721 OX=2558815 GN=JavanS721_0007 PE=4 SV=1
MM1 pKa = 7.5 NEE3 pKa = 3.99 LDD5 pKa = 3.58 ISNTQAVFVTVILIGLLLYY24 pKa = 10.81 LNHH27 pKa = 7.57 LDD29 pKa = 3.74 RR30 pKa = 11.84 KK31 pKa = 10.37 KK32 pKa = 10.35 SAQLEE37 pKa = 4.35 RR38 pKa = 11.84 EE39 pKa = 4.1 NQQTIEE45 pKa = 4.44 PPSEE49 pKa = 4.4 DD50 pKa = 4.04 LNPDD54 pKa = 3.22 YY55 pKa = 11.28 GRR57 pKa = 11.84 YY58 pKa = 8.57 IQLAGVDD65 pKa = 3.08 VWGRR69 pKa = 11.84 IEE71 pKa = 3.73
Molecular weight: 8.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.599
IPC2_protein 4.609
IPC_protein 4.431
Toseland 4.279
ProMoST 4.444
Dawson 4.38
Bjellqvist 4.622
Wikipedia 4.266
Rodwell 4.266
Grimsley 4.19
Solomon 4.368
Lehninger 4.329
Nozaki 4.507
DTASelect 4.647
Thurlkill 4.291
EMBOSS 4.279
Sillero 4.546
Patrickios 3.897
IPC_peptide 4.38
IPC2_peptide 4.533
IPC2.peptide.svr19 4.502
Protein with the highest isoelectric point:
>tr|A0A4D5ZTK1|A0A4D5ZTK1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan721 OX=2558815 GN=JavanS721_0015 PE=4 SV=1
MM1 pKa = 7.67 PPLASFLASKK11 pKa = 8.77 TALASVLILFTTCLYY26 pKa = 10.63 KK27 pKa = 10.29 RR28 pKa = 11.84 VRR30 pKa = 11.84 GLYY33 pKa = 9.53 NGLYY37 pKa = 9.84 RR38 pKa = 11.84 CIDD41 pKa = 3.34 ILRR44 pKa = 11.84 GIVALFSNIIQDD56 pKa = 4.09 LFNPFLEE63 pKa = 4.43 INKK66 pKa = 8.35 ITSSVLLASALSEE79 pKa = 4.31 TNRR82 pKa = 11.84 MSMPGRR88 pKa = 3.74
Molecular weight: 9.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.241
IPC2_protein 9.238
IPC_protein 9.472
Toseland 9.692
ProMoST 9.823
Dawson 9.984
Bjellqvist 9.78
Wikipedia 10.204
Rodwell 10.16
Grimsley 10.072
Solomon 10.072
Lehninger 10.028
Nozaki 9.853
DTASelect 9.736
Thurlkill 9.809
EMBOSS 10.116
Sillero 9.94
Patrickios 7.556
IPC_peptide 10.058
IPC2_peptide 8.916
IPC2.peptide.svr19 8.162
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2501
57
387
147.1
16.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.397 ± 0.412
0.92 ± 0.162
5.278 ± 0.393
8.637 ± 1.025
3.519 ± 0.318
4.158 ± 0.408
1.479 ± 0.279
6.957 ± 0.344
10.076 ± 0.463
10.796 ± 0.647
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.959 ± 0.354
5.638 ± 0.554
2.399 ± 0.252
4.758 ± 0.288
3.878 ± 0.309
5.518 ± 0.281
6.238 ± 0.347
4.918 ± 0.617
1.0 ± 0.168
4.478 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here