Tomato leaf curl alphasatellite

Taxonomy: Viruses; Alphasatellitidae; Geminialphasatellitinae; Colecusatellite; Tomato leaf curl Pakistan alphasatellite

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2K8HQA9|A0A2K8HQA9_9VIRU RepA OS=Tomato leaf curl alphasatellite OX=1407057 PE=4 SV=1
MM1 pKa = 7.81PALKK5 pKa = 10.38SQWWCFTVFFVSASAPDD22 pKa = 3.92LVPLFEE28 pKa = 4.5NTHH31 pKa = 4.91VSYY34 pKa = 11.22ACWQEE39 pKa = 4.16EE40 pKa = 4.12EE41 pKa = 4.64SPTTKK46 pKa = 9.77RR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 4.72LQGYY53 pKa = 8.29LQLKK57 pKa = 7.47GQRR60 pKa = 11.84TLNQVKK66 pKa = 10.54AIFGDD71 pKa = 4.27LKK73 pKa = 10.8PHH75 pKa = 6.48LEE77 pKa = 3.68KK78 pKa = 10.83HH79 pKa = 5.32RR80 pKa = 11.84ARR82 pKa = 11.84NIDD85 pKa = 3.48DD86 pKa = 3.85ARR88 pKa = 11.84DD89 pKa = 3.35YY90 pKa = 11.71CMNPEE95 pKa = 4.17TRR97 pKa = 11.84VSCPFEE103 pKa = 3.71FWEE106 pKa = 4.59FCPAGSHH113 pKa = 6.29KK114 pKa = 10.18RR115 pKa = 11.84RR116 pKa = 11.84QRR118 pKa = 11.84EE119 pKa = 3.66LVIRR123 pKa = 11.84YY124 pKa = 6.18PVRR127 pKa = 11.84MAEE130 pKa = 4.17EE131 pKa = 4.18NPSVFRR137 pKa = 11.84RR138 pKa = 11.84VKK140 pKa = 10.56AKK142 pKa = 9.83IAEE145 pKa = 4.16EE146 pKa = 4.19EE147 pKa = 4.27FQKK150 pKa = 10.71SAPEE154 pKa = 3.79IQISNLKK161 pKa = 9.56SWQSRR166 pKa = 11.84LKK168 pKa = 9.88TLLEE172 pKa = 4.45RR173 pKa = 11.84DD174 pKa = 3.49PDD176 pKa = 3.57DD177 pKa = 3.89RR178 pKa = 11.84TIFWVYY184 pKa = 10.77GPDD187 pKa = 3.41GGEE190 pKa = 4.15GKK192 pKa = 8.42STFARR197 pKa = 11.84DD198 pKa = 3.99LYY200 pKa = 11.15RR201 pKa = 11.84SGSWFYY207 pKa = 11.09TRR209 pKa = 11.84GGSADD214 pKa = 3.51NVV216 pKa = 3.66

Molecular weight:
25.27 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2K8HQA9|A0A2K8HQA9_9VIRU RepA OS=Tomato leaf curl alphasatellite OX=1407057 PE=4 SV=1
MM1 pKa = 7.81PALKK5 pKa = 10.38SQWWCFTVFFVSASAPDD22 pKa = 3.92LVPLFEE28 pKa = 4.5NTHH31 pKa = 4.91VSYY34 pKa = 11.22ACWQEE39 pKa = 4.16EE40 pKa = 4.12EE41 pKa = 4.64SPTTKK46 pKa = 9.77RR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 4.72LQGYY53 pKa = 8.29LQLKK57 pKa = 7.47GQRR60 pKa = 11.84TLNQVKK66 pKa = 10.54AIFGDD71 pKa = 4.27LKK73 pKa = 10.8PHH75 pKa = 6.48LEE77 pKa = 3.68KK78 pKa = 10.83HH79 pKa = 5.32RR80 pKa = 11.84ARR82 pKa = 11.84NIDD85 pKa = 3.48DD86 pKa = 3.85ARR88 pKa = 11.84DD89 pKa = 3.35YY90 pKa = 11.71CMNPEE95 pKa = 4.17TRR97 pKa = 11.84VSCPFEE103 pKa = 3.71FWEE106 pKa = 4.59FCPAGSHH113 pKa = 6.29KK114 pKa = 10.18RR115 pKa = 11.84RR116 pKa = 11.84QRR118 pKa = 11.84EE119 pKa = 3.66LVIRR123 pKa = 11.84YY124 pKa = 6.18PVRR127 pKa = 11.84MAEE130 pKa = 4.17EE131 pKa = 4.18NPSVFRR137 pKa = 11.84RR138 pKa = 11.84VKK140 pKa = 10.56AKK142 pKa = 9.83IAEE145 pKa = 4.16EE146 pKa = 4.19EE147 pKa = 4.27FQKK150 pKa = 10.71SAPEE154 pKa = 3.79IQISNLKK161 pKa = 9.56SWQSRR166 pKa = 11.84LKK168 pKa = 9.88TLLEE172 pKa = 4.45RR173 pKa = 11.84DD174 pKa = 3.49PDD176 pKa = 3.57DD177 pKa = 3.89RR178 pKa = 11.84TIFWVYY184 pKa = 10.77GPDD187 pKa = 3.41GGEE190 pKa = 4.15GKK192 pKa = 8.42STFARR197 pKa = 11.84DD198 pKa = 3.99LYY200 pKa = 11.15RR201 pKa = 11.84SGSWFYY207 pKa = 11.09TRR209 pKa = 11.84GGSADD214 pKa = 3.51NVV216 pKa = 3.66

Molecular weight:
25.27 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

216

216

216

216.0

25.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.481 ± 0.0

2.315 ± 0.0

5.093 ± 0.0

7.87 ± 0.0

6.019 ± 0.0

5.093 ± 0.0

2.315 ± 0.0

3.241 ± 0.0

6.019 ± 0.0

6.944 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.389 ± 0.0

3.241 ± 0.0

6.019 ± 0.0

4.63 ± 0.0

9.259 ± 0.0

7.407 ± 0.0

4.63 ± 0.0

5.556 ± 0.0

3.241 ± 0.0

3.241 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski