Tomato leaf curl alphasatellite
Average proteome isoelectric point is 7.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K8HQA9|A0A2K8HQA9_9VIRU RepA OS=Tomato leaf curl alphasatellite OX=1407057 PE=4 SV=1
MM1 pKa = 7.81 PALKK5 pKa = 10.38 SQWWCFTVFFVSASAPDD22 pKa = 3.92 LVPLFEE28 pKa = 4.5 NTHH31 pKa = 4.91 VSYY34 pKa = 11.22 ACWQEE39 pKa = 4.16 EE40 pKa = 4.12 EE41 pKa = 4.64 SPTTKK46 pKa = 9.77 RR47 pKa = 11.84 RR48 pKa = 11.84 HH49 pKa = 4.72 LQGYY53 pKa = 8.29 LQLKK57 pKa = 7.47 GQRR60 pKa = 11.84 TLNQVKK66 pKa = 10.54 AIFGDD71 pKa = 4.27 LKK73 pKa = 10.8 PHH75 pKa = 6.48 LEE77 pKa = 3.68 KK78 pKa = 10.83 HH79 pKa = 5.32 RR80 pKa = 11.84 ARR82 pKa = 11.84 NIDD85 pKa = 3.48 DD86 pKa = 3.85 ARR88 pKa = 11.84 DD89 pKa = 3.35 YY90 pKa = 11.71 CMNPEE95 pKa = 4.17 TRR97 pKa = 11.84 VSCPFEE103 pKa = 3.71 FWEE106 pKa = 4.59 FCPAGSHH113 pKa = 6.29 KK114 pKa = 10.18 RR115 pKa = 11.84 RR116 pKa = 11.84 QRR118 pKa = 11.84 EE119 pKa = 3.66 LVIRR123 pKa = 11.84 YY124 pKa = 6.18 PVRR127 pKa = 11.84 MAEE130 pKa = 4.17 EE131 pKa = 4.18 NPSVFRR137 pKa = 11.84 RR138 pKa = 11.84 VKK140 pKa = 10.56 AKK142 pKa = 9.83 IAEE145 pKa = 4.16 EE146 pKa = 4.19 EE147 pKa = 4.27 FQKK150 pKa = 10.71 SAPEE154 pKa = 3.79 IQISNLKK161 pKa = 9.56 SWQSRR166 pKa = 11.84 LKK168 pKa = 9.88 TLLEE172 pKa = 4.45 RR173 pKa = 11.84 DD174 pKa = 3.49 PDD176 pKa = 3.57 DD177 pKa = 3.89 RR178 pKa = 11.84 TIFWVYY184 pKa = 10.77 GPDD187 pKa = 3.41 GGEE190 pKa = 4.15 GKK192 pKa = 8.42 STFARR197 pKa = 11.84 DD198 pKa = 3.99 LYY200 pKa = 11.15 RR201 pKa = 11.84 SGSWFYY207 pKa = 11.09 TRR209 pKa = 11.84 GGSADD214 pKa = 3.51 NVV216 pKa = 3.66
Molecular weight: 25.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.983
IPC2_protein 8.083
IPC_protein 8.039
Toseland 8.039
ProMoST 8.507
Dawson 8.756
Bjellqvist 8.946
Wikipedia 8.726
Rodwell 8.799
Grimsley 7.863
Solomon 8.931
Lehninger 8.916
Nozaki 9.136
DTASelect 8.726
Thurlkill 8.814
EMBOSS 8.946
Sillero 9.092
Patrickios 4.698
IPC_peptide 8.916
IPC2_peptide 7.863
IPC2.peptide.svr19 7.854
Protein with the highest isoelectric point:
>tr|A0A2K8HQA9|A0A2K8HQA9_9VIRU RepA OS=Tomato leaf curl alphasatellite OX=1407057 PE=4 SV=1
MM1 pKa = 7.81 PALKK5 pKa = 10.38 SQWWCFTVFFVSASAPDD22 pKa = 3.92 LVPLFEE28 pKa = 4.5 NTHH31 pKa = 4.91 VSYY34 pKa = 11.22 ACWQEE39 pKa = 4.16 EE40 pKa = 4.12 EE41 pKa = 4.64 SPTTKK46 pKa = 9.77 RR47 pKa = 11.84 RR48 pKa = 11.84 HH49 pKa = 4.72 LQGYY53 pKa = 8.29 LQLKK57 pKa = 7.47 GQRR60 pKa = 11.84 TLNQVKK66 pKa = 10.54 AIFGDD71 pKa = 4.27 LKK73 pKa = 10.8 PHH75 pKa = 6.48 LEE77 pKa = 3.68 KK78 pKa = 10.83 HH79 pKa = 5.32 RR80 pKa = 11.84 ARR82 pKa = 11.84 NIDD85 pKa = 3.48 DD86 pKa = 3.85 ARR88 pKa = 11.84 DD89 pKa = 3.35 YY90 pKa = 11.71 CMNPEE95 pKa = 4.17 TRR97 pKa = 11.84 VSCPFEE103 pKa = 3.71 FWEE106 pKa = 4.59 FCPAGSHH113 pKa = 6.29 KK114 pKa = 10.18 RR115 pKa = 11.84 RR116 pKa = 11.84 QRR118 pKa = 11.84 EE119 pKa = 3.66 LVIRR123 pKa = 11.84 YY124 pKa = 6.18 PVRR127 pKa = 11.84 MAEE130 pKa = 4.17 EE131 pKa = 4.18 NPSVFRR137 pKa = 11.84 RR138 pKa = 11.84 VKK140 pKa = 10.56 AKK142 pKa = 9.83 IAEE145 pKa = 4.16 EE146 pKa = 4.19 EE147 pKa = 4.27 FQKK150 pKa = 10.71 SAPEE154 pKa = 3.79 IQISNLKK161 pKa = 9.56 SWQSRR166 pKa = 11.84 LKK168 pKa = 9.88 TLLEE172 pKa = 4.45 RR173 pKa = 11.84 DD174 pKa = 3.49 PDD176 pKa = 3.57 DD177 pKa = 3.89 RR178 pKa = 11.84 TIFWVYY184 pKa = 10.77 GPDD187 pKa = 3.41 GGEE190 pKa = 4.15 GKK192 pKa = 8.42 STFARR197 pKa = 11.84 DD198 pKa = 3.99 LYY200 pKa = 11.15 RR201 pKa = 11.84 SGSWFYY207 pKa = 11.09 TRR209 pKa = 11.84 GGSADD214 pKa = 3.51 NVV216 pKa = 3.66
Molecular weight: 25.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.983
IPC2_protein 8.083
IPC_protein 8.039
Toseland 8.039
ProMoST 8.507
Dawson 8.756
Bjellqvist 8.946
Wikipedia 8.726
Rodwell 8.799
Grimsley 7.863
Solomon 8.931
Lehninger 8.916
Nozaki 9.136
DTASelect 8.726
Thurlkill 8.814
EMBOSS 8.946
Sillero 9.092
Patrickios 4.698
IPC_peptide 8.916
IPC2_peptide 7.863
IPC2.peptide.svr19 7.854
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
216
216
216
216.0
25.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.481 ± 0.0
2.315 ± 0.0
5.093 ± 0.0
7.87 ± 0.0
6.019 ± 0.0
5.093 ± 0.0
2.315 ± 0.0
3.241 ± 0.0
6.019 ± 0.0
6.944 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.389 ± 0.0
3.241 ± 0.0
6.019 ± 0.0
4.63 ± 0.0
9.259 ± 0.0
7.407 ± 0.0
4.63 ± 0.0
5.556 ± 0.0
3.241 ± 0.0
3.241 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here