Streptococcus cristatus
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1914 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F2CXP3|A0A0F2CXP3_STRCR ABC transporter ATP-binding protein OS=Streptococcus cristatus OX=45634 GN=TW70_00263 PE=4 SV=1
MM1 pKa = 7.62 EE2 pKa = 6.25 LLDD5 pKa = 4.04 KK6 pKa = 10.96 YY7 pKa = 10.91 KK8 pKa = 10.33 KK9 pKa = 10.35 EE10 pKa = 3.92 KK11 pKa = 10.77 VIDD14 pKa = 4.21 VITNILKK21 pKa = 10.52 AIHH24 pKa = 6.01 LKK26 pKa = 10.43 KK27 pKa = 10.98 YY28 pKa = 9.44 EE29 pKa = 4.78 DD30 pKa = 3.11 IMNYY34 pKa = 9.55 VDD36 pKa = 4.23 EE37 pKa = 5.62 SEE39 pKa = 4.88 IDD41 pKa = 3.58 DD42 pKa = 4.09 LNEE45 pKa = 3.39 FFGYY49 pKa = 9.21 VEE51 pKa = 4.51 KK52 pKa = 10.18 TLEE55 pKa = 4.37 LNDD58 pKa = 3.85 FDD60 pKa = 5.62 TIDD63 pKa = 3.49 EE64 pKa = 4.57 YY65 pKa = 11.67 GVEE68 pKa = 4.33 CHH70 pKa = 6.55 FNPPYY75 pKa = 10.3 EE76 pKa = 4.0 YY77 pKa = 10.47 SQLEE81 pKa = 4.14 IYY83 pKa = 10.48 DD84 pKa = 4.15 YY85 pKa = 11.7 DD86 pKa = 4.04 DD87 pKa = 3.29 QTGFAVDD94 pKa = 4.12 YY95 pKa = 10.8 DD96 pKa = 3.99 LTSNSGLVDD105 pKa = 3.17 MRR107 pKa = 11.84 LQLEE111 pKa = 4.26 FLYY114 pKa = 10.39 TDD116 pKa = 2.53 NGYY119 pKa = 7.42 TVRR122 pKa = 11.84 FLNVDD127 pKa = 2.99 SDD129 pKa = 3.81
Molecular weight: 15.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.897
IPC_protein 3.884
Toseland 3.668
ProMoST 3.999
Dawson 3.872
Bjellqvist 4.05
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.202
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 0.807
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A0F2CGR4|A0A0F2CGR4_STRCR Thiamin pyrophosphokinase OS=Streptococcus cristatus OX=45634 GN=thiN PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.24 QPSKK9 pKa = 9.82 LRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.57 HH16 pKa = 4.75 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 9.22 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLAAA44 pKa = 4.31
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1914
0
1914
584487
33
3871
305.4
34.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.882 ± 0.063
0.509 ± 0.014
5.644 ± 0.056
7.33 ± 0.073
4.58 ± 0.055
6.567 ± 0.084
1.858 ± 0.023
7.006 ± 0.062
6.687 ± 0.049
10.357 ± 0.116
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.396 ± 0.031
4.234 ± 0.042
3.388 ± 0.038
4.248 ± 0.045
4.133 ± 0.043
6.239 ± 0.071
5.427 ± 0.061
6.862 ± 0.053
0.899 ± 0.019
3.754 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here