Planococcus rifietoensis
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3386 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U2YV83|A0A0U2YV83_9BACL RNA methyltransferase TrmH OS=Planococcus rifietoensis OX=200991 GN=AUC31_09775 PE=4 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.37 KK3 pKa = 10.32 FNLMFLAAASMAALAACSSGDD24 pKa = 3.57 EE25 pKa = 4.23 EE26 pKa = 5.1 ASGGSGSEE34 pKa = 4.07 GSADD38 pKa = 3.41 VDD40 pKa = 5.55 EE41 pKa = 4.52 ITAWAWDD48 pKa = 3.58 PAFNIAALEE57 pKa = 4.13 NAKK60 pKa = 9.89 EE61 pKa = 4.09 AYY63 pKa = 10.29 NGDD66 pKa = 2.9 GDD68 pKa = 4.27 YY69 pKa = 11.04 EE70 pKa = 4.56 VNIIEE75 pKa = 4.5 NAQDD79 pKa = 5.51 DD80 pKa = 4.9 IIQKK84 pKa = 10.54 LNTGLSSGTTNGMPNIVLIEE104 pKa = 4.37 DD105 pKa = 3.41 YY106 pKa = 10.36 RR107 pKa = 11.84 AQSFLQAYY115 pKa = 8.23 PDD117 pKa = 3.89 AFHH120 pKa = 7.54 PLTDD124 pKa = 4.61 VINSDD129 pKa = 4.22 DD130 pKa = 3.72 FADD133 pKa = 3.78 YY134 pKa = 10.8 KK135 pKa = 10.78 IATTSYY141 pKa = 10.91 DD142 pKa = 3.31 GEE144 pKa = 4.49 QYY146 pKa = 11.35 GLPFDD151 pKa = 4.24 SGVAALYY158 pKa = 10.74 VRR160 pKa = 11.84 TDD162 pKa = 3.48 YY163 pKa = 11.52 LEE165 pKa = 4.05 EE166 pKa = 5.61 AGYY169 pKa = 10.44 SVEE172 pKa = 4.85 DD173 pKa = 3.52 VTDD176 pKa = 3.66 ITWQEE181 pKa = 3.99 YY182 pKa = 10.34 IEE184 pKa = 4.35 IGKK187 pKa = 9.3 DD188 pKa = 3.19 VKK190 pKa = 10.77 EE191 pKa = 4.02 ATGKK195 pKa = 10.46 NMLTLDD201 pKa = 4.5 PNDD204 pKa = 3.71 LAQVRR209 pKa = 11.84 MMIQTNGSWYY219 pKa = 10.17 VEE221 pKa = 4.27 EE222 pKa = 5.93 DD223 pKa = 3.24 GSTPNIAGNEE233 pKa = 4.01 TLEE236 pKa = 4.98 AGFEE240 pKa = 4.5 TYY242 pKa = 10.74 KK243 pKa = 11.25 EE244 pKa = 4.05 MMDD247 pKa = 3.43 ADD249 pKa = 3.42 IAKK252 pKa = 9.81 IVSDD256 pKa = 3.53 WSQFVGAFNSGEE268 pKa = 4.24 VASVPTGNWITPSVKK283 pKa = 9.92 QAADD287 pKa = 3.49 QSGKK291 pKa = 8.65 WAVVPMPRR299 pKa = 11.84 LDD301 pKa = 3.44 MEE303 pKa = 4.69 GAVNASNLGGSSWYY317 pKa = 9.91 VLNVDD322 pKa = 4.65 GKK324 pKa = 9.78 EE325 pKa = 3.91 QAADD329 pKa = 3.56 FLLNTFGSNPEE340 pKa = 4.13 LYY342 pKa = 8.53 QTLVEE347 pKa = 5.37 DD348 pKa = 3.86 IGALGTYY355 pKa = 10.03 SPAAEE360 pKa = 4.02 GDD362 pKa = 3.47 AYY364 pKa = 10.64 AVEE367 pKa = 5.1 DD368 pKa = 4.37 EE369 pKa = 4.8 FFGGQQLLTDD379 pKa = 4.45 LSTWTDD385 pKa = 3.62 EE386 pKa = 4.3 IPAVNYY392 pKa = 10.0 GLHH395 pKa = 6.22 TYY397 pKa = 10.28 AIEE400 pKa = 5.42 DD401 pKa = 3.55 ILVTGMQDD409 pKa = 3.54 YY410 pKa = 11.43 LNGAEE415 pKa = 4.93 LDD417 pKa = 3.98 TVLEE421 pKa = 4.19 NVQGQAEE428 pKa = 4.28 AQLKK432 pKa = 9.34
Molecular weight: 46.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.656
IPC_protein 3.668
Toseland 3.452
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.49
Grimsley 3.363
Solomon 3.643
Lehninger 3.605
Nozaki 3.757
DTASelect 3.986
Thurlkill 3.503
EMBOSS 3.579
Sillero 3.795
Patrickios 1.011
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.697
Protein with the highest isoelectric point:
>tr|A0A0U2Z9T2|A0A0U2Z9T2_9BACL GTPase Der OS=Planococcus rifietoensis OX=200991 GN=engA PE=3 SV=1
MM1 pKa = 7.61 TLRR4 pKa = 11.84 TYY6 pKa = 10.57 QPNKK10 pKa = 8.74 RR11 pKa = 11.84 KK12 pKa = 9.54 HH13 pKa = 5.99 SKK15 pKa = 8.91 VHH17 pKa = 5.68 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSTKK26 pKa = 9.71 NGRR29 pKa = 11.84 RR30 pKa = 11.84 VIAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.8 GRR40 pKa = 11.84 KK41 pKa = 8.75 VLSAA45 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3386
0
3386
996273
37
1561
294.2
32.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.555 ± 0.041
0.549 ± 0.011
5.416 ± 0.038
7.962 ± 0.054
4.506 ± 0.037
7.318 ± 0.04
2.067 ± 0.022
6.948 ± 0.039
5.619 ± 0.035
9.906 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.916 ± 0.02
3.569 ± 0.025
3.82 ± 0.026
3.861 ± 0.028
4.417 ± 0.032
5.851 ± 0.025
5.337 ± 0.026
7.111 ± 0.028
1.052 ± 0.017
3.22 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here