Caldibacillus debilis
Average proteome isoelectric point is 7.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4240 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A150M7X3|A0A150M7X3_9BACI 30S ribosomal protein S4 OS=Caldibacillus debilis OX=301148 GN=rpsD PE=3 SV=1
MM1 pKa = 7.14 VCVDD5 pKa = 4.47 KK6 pKa = 11.44 NQEE9 pKa = 4.02 EE10 pKa = 4.82 YY11 pKa = 11.59 GMEE14 pKa = 4.11 SWNKK18 pKa = 8.98 TIYY21 pKa = 10.5 KK22 pKa = 9.78 EE23 pKa = 3.96 IIEE26 pKa = 4.33 PEE28 pKa = 4.05 KK29 pKa = 10.43 IVYY32 pKa = 9.39 IDD34 pKa = 3.8 YY35 pKa = 10.94 FSDD38 pKa = 3.81 SEE40 pKa = 4.77 GNMSS44 pKa = 3.79
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.942
IPC2_protein 4.279
IPC_protein 4.075
Toseland 3.923
ProMoST 4.202
Dawson 4.024
Bjellqvist 4.19
Wikipedia 3.91
Rodwell 3.923
Grimsley 3.846
Solomon 3.999
Lehninger 3.961
Nozaki 4.151
DTASelect 4.24
Thurlkill 3.961
EMBOSS 3.923
Sillero 4.19
Patrickios 1.926
IPC_peptide 4.012
IPC2_peptide 4.164
IPC2.peptide.svr19 4.085
Protein with the highest isoelectric point:
>tr|A0A150L9Q0|A0A150L9Q0_9BACI Transketolase N-terminal section OS=Caldibacillus debilis OX=301148 GN=B4135_4149 PE=4 SV=1
MM1 pKa = 7.34 KK2 pKa = 10.42 ASGGRR7 pKa = 11.84 SRR9 pKa = 11.84 VKK11 pKa = 10.05 IRR13 pKa = 11.84 PGNPAGATRR22 pKa = 11.84 SARR25 pKa = 11.84 RR26 pKa = 11.84 APFSAFGGKK35 pKa = 9.54 GRR37 pKa = 11.84 ILLNLL42 pKa = 3.7
Molecular weight: 4.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.471
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.457
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.193
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4240
0
4240
910805
37
2865
214.8
24.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.636 ± 0.046
0.849 ± 0.012
4.737 ± 0.032
7.63 ± 0.057
5.071 ± 0.048
7.627 ± 0.042
1.981 ± 0.017
7.019 ± 0.041
7.142 ± 0.037
9.675 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.645 ± 0.021
3.627 ± 0.03
4.53 ± 0.031
3.088 ± 0.025
6.046 ± 0.044
5.342 ± 0.035
4.562 ± 0.026
6.491 ± 0.033
1.059 ± 0.016
3.244 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here