Po-Circo-like virus 22

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae; Po-Circo-like virus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8E3Y0|G8E3Y0_9CIRC Uncharacterized protein OS=Po-Circo-like virus 22 OX=1105384 PE=4 SV=1
MM1 pKa = 7.17SQDD4 pKa = 3.26FDD6 pKa = 4.02VFDD9 pKa = 4.9PFSDD13 pKa = 4.24YY14 pKa = 11.12IPDD17 pKa = 4.08FSQYY21 pKa = 11.24GFDD24 pKa = 3.64EE25 pKa = 4.94ASSQVLDD32 pKa = 4.01PSGNPYY38 pKa = 7.81EE39 pKa = 4.36TQITVPFMQSVEE51 pKa = 4.16QGSDD55 pKa = 3.09PSVTLRR61 pKa = 11.84SMLEE65 pKa = 3.51QEE67 pKa = 4.35YY68 pKa = 10.17QSNRR72 pKa = 11.84RR73 pKa = 11.84EE74 pKa = 4.12VTDD77 pKa = 3.72PTDD80 pKa = 4.38VMPQDD85 pKa = 3.52APVFRR90 pKa = 11.84RR91 pKa = 11.84LDD93 pKa = 3.59EE94 pKa = 4.38NSVLFHH100 pKa = 7.12DD101 pKa = 4.71VSFAGPTLRR110 pKa = 11.84SEE112 pKa = 4.47QQCKK116 pKa = 7.53TRR118 pKa = 11.84EE119 pKa = 4.05LQGFLILPARR129 pKa = 11.84FCTAIRR135 pKa = 11.84SRR137 pKa = 11.84AGNHH141 pKa = 5.85SLPTPALFVKK151 pKa = 10.16EE152 pKa = 4.22LDD154 pKa = 3.78SSISALFEE162 pKa = 3.97

Molecular weight: 18.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8E3Y0|G8E3Y0_9CIRC Uncharacterized protein OS=Po-Circo-like virus 22 OX=1105384 PE=4 SV=1
MM1 pKa = 7.43PRR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 7.97TRR7 pKa = 11.84SGRR10 pKa = 11.84YY11 pKa = 5.31TRR13 pKa = 11.84RR14 pKa = 11.84VKK16 pKa = 8.83TVKK19 pKa = 10.5YY20 pKa = 10.86SNEE23 pKa = 3.92TYY25 pKa = 10.7NYY27 pKa = 9.04AGLYY31 pKa = 7.26TVQNAGVKK39 pKa = 9.85NNWSEE44 pKa = 4.11TLISAIEE51 pKa = 4.02SQGVRR56 pKa = 11.84KK57 pKa = 10.0CKK59 pKa = 10.41NFEE62 pKa = 4.09LSLTGTPFEE71 pKa = 4.18NAQNQVAHH79 pKa = 6.68APMFFALVYY88 pKa = 9.76VPQGTEE94 pKa = 3.51PSQINIGNPDD104 pKa = 3.32ACASLYY110 pKa = 10.48EE111 pKa = 4.36PNQNVIMSGVWPSDD125 pKa = 2.98LTAPFVKK132 pKa = 9.34KK133 pKa = 9.03TRR135 pKa = 11.84LARR138 pKa = 11.84NLNSGDD144 pKa = 3.86RR145 pKa = 11.84IVLVLSTPQFGEE157 pKa = 3.96GAYY160 pKa = 10.4SKK162 pKa = 11.16NLAVTLNYY170 pKa = 10.43AISFF174 pKa = 4.05

Molecular weight: 19.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

832

162

310

208.0

23.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.25 ± 0.461

1.202 ± 0.044

4.928 ± 0.981

6.971 ± 1.615

4.928 ± 0.769

5.769 ± 0.521

1.683 ± 0.517

6.01 ± 1.124

4.928 ± 1.201

7.212 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.043 ± 0.161

5.649 ± 0.946

5.409 ± 0.79

5.168 ± 0.532

5.889 ± 0.312

7.332 ± 1.824

6.37 ± 0.484

6.13 ± 0.62

1.442 ± 0.777

4.688 ± 0.769
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski