Nonomuraea maritima
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7903 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G9NBA0|A0A1G9NBA0_9ACTN 1 4-dihydroxy-2-naphthoyl-CoA synthase OS=Nonomuraea maritima OX=683260 GN=menB PE=3 SV=1
MM1 pKa = 7.8 TDD3 pKa = 3.0 NDD5 pKa = 4.17 LTFGDD10 pKa = 4.67 EE11 pKa = 4.68 DD12 pKa = 4.4 YY13 pKa = 11.19 EE14 pKa = 4.67 LPDD17 pKa = 3.25 VEE19 pKa = 5.9 RR20 pKa = 11.84 RR21 pKa = 11.84 ICPSCGTNSRR31 pKa = 11.84 IITRR35 pKa = 11.84 GDD37 pKa = 2.95 SRR39 pKa = 11.84 ATIVSKK45 pKa = 10.93 CCEE48 pKa = 4.05 APLSGTADD56 pKa = 3.79 DD57 pKa = 5.22 QDD59 pKa = 4.6 DD60 pKa = 4.27 EE61 pKa = 4.64 PAVLVLDD68 pKa = 4.79 GKK70 pKa = 9.44 LVCPYY75 pKa = 10.08 ADD77 pKa = 4.02 CSATDD82 pKa = 4.16 EE83 pKa = 4.33 IVEE86 pKa = 4.32 LDD88 pKa = 3.31 VATRR92 pKa = 11.84 SNEE95 pKa = 3.67 LTIIRR100 pKa = 11.84 PGVISVALGDD110 pKa = 3.88 SCFEE114 pKa = 4.07 NDD116 pKa = 4.06 GFEE119 pKa = 4.8 CEE121 pKa = 3.99 QCSRR125 pKa = 11.84 RR126 pKa = 11.84 VSLPDD131 pKa = 3.82 GYY133 pKa = 10.96 EE134 pKa = 3.44 LSYY137 pKa = 10.91 PP138 pKa = 3.76
Molecular weight: 15.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.656
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.694
Grimsley 3.567
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.706
EMBOSS 3.795
Sillero 3.986
Patrickios 1.074
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.864
Protein with the highest isoelectric point:
>tr|A0A1G8UDI3|A0A1G8UDI3_9ACTN Bifunctional protein GlmU OS=Nonomuraea maritima OX=683260 GN=glmU PE=3 SV=1
MM1 pKa = 6.33 QWRR4 pKa = 11.84 RR5 pKa = 11.84 SGYY8 pKa = 8.05 TSSLRR13 pKa = 11.84 SSASRR18 pKa = 11.84 ARR20 pKa = 11.84 SSWPSSRR27 pKa = 11.84 RR28 pKa = 11.84 WGSSSVRR35 pKa = 11.84 RR36 pKa = 11.84 PRR38 pKa = 11.84 PSRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 WSAGSPKK50 pKa = 9.85 LWAPRR55 pKa = 11.84 RR56 pKa = 11.84 VALPGAAVSRR66 pKa = 11.84 PTSRR70 pKa = 11.84 SRR72 pKa = 11.84 QGLRR76 pKa = 11.84 PGRR79 pKa = 11.84 AMARR83 pKa = 11.84 PATARR88 pKa = 11.84 RR89 pKa = 11.84 RR90 pKa = 11.84 RR91 pKa = 11.84 FPLRR95 pKa = 11.84 VRR97 pKa = 11.84 APVPVRR103 pKa = 11.84 VPAPQALRR111 pKa = 11.84 APQAARR117 pKa = 11.84 SRR119 pKa = 11.84 VRR121 pKa = 11.84 RR122 pKa = 11.84 FRR124 pKa = 11.84 CPTSRR129 pKa = 11.84 SSSPRR134 pKa = 11.84 PRR136 pKa = 11.84 AVRR139 pKa = 11.84 PPGLRR144 pKa = 11.84 RR145 pKa = 11.84 RR146 pKa = 11.84 ASTVARR152 pKa = 11.84 PLVRR156 pKa = 11.84 LRR158 pKa = 11.84 ARR160 pKa = 11.84 VPAPPPVPARR170 pKa = 11.84 SPALGPVRR178 pKa = 11.84 VLVVAVAVAVPRR190 pKa = 11.84 RR191 pKa = 11.84 RR192 pKa = 11.84 RR193 pKa = 11.84 VPARR197 pKa = 11.84 LPVAPLVPARR207 pKa = 11.84 APSPARR213 pKa = 11.84 VVRR216 pKa = 11.84 VPATTRR222 pKa = 11.84 SRR224 pKa = 11.84 PTRR227 pKa = 11.84 AAWVSRR233 pKa = 11.84 VRR235 pKa = 11.84 RR236 pKa = 11.84 VPVATAKK243 pKa = 10.54 AAAPASASVVTVRR256 pKa = 11.84 RR257 pKa = 11.84 ATARR261 pKa = 11.84 CRR263 pKa = 11.84 VRR265 pKa = 11.84 RR266 pKa = 11.84 RR267 pKa = 11.84 AVPAPTPGPAPAARR281 pKa = 11.84 VPARR285 pKa = 11.84 RR286 pKa = 11.84 PAVRR290 pKa = 11.84 VRR292 pKa = 11.84 VPVRR296 pKa = 11.84 VVRR299 pKa = 11.84 VRR301 pKa = 11.84 VARR304 pKa = 11.84 VPTRR308 pKa = 3.2
Molecular weight: 33.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.498
IPC2_protein 11.55
IPC_protein 13.115
Toseland 13.261
ProMoST 13.773
Dawson 13.261
Bjellqvist 13.261
Wikipedia 13.744
Rodwell 12.778
Grimsley 13.305
Solomon 13.773
Lehninger 13.671
Nozaki 13.261
DTASelect 13.261
Thurlkill 13.261
EMBOSS 13.773
Sillero 13.261
Patrickios 12.486
IPC_peptide 13.773
IPC2_peptide 12.764
IPC2.peptide.svr19 9.399
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7903
0
7903
2450462
26
3110
310.1
33.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.225 ± 0.04
0.78 ± 0.009
5.887 ± 0.022
5.683 ± 0.025
2.8 ± 0.015
9.189 ± 0.028
2.272 ± 0.011
3.426 ± 0.018
2.018 ± 0.019
10.591 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.935 ± 0.012
1.722 ± 0.013
6.042 ± 0.026
2.7 ± 0.016
8.302 ± 0.03
4.954 ± 0.02
5.806 ± 0.021
9.015 ± 0.027
1.548 ± 0.011
2.106 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here