African oil palm ringspot virus
Average proteome isoelectric point is 7.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C1BEG0|C1BEG0_9VIRU Movement protein TGB2 OS=African oil palm ringspot virus OX=185218 PE=3 SV=1
MM1 pKa = 7.69 EE2 pKa = 4.65 EE3 pKa = 3.91 VCEE6 pKa = 4.22 KK7 pKa = 10.69 LLEE10 pKa = 4.32 LGYY13 pKa = 10.34 IRR15 pKa = 11.84 VEE17 pKa = 4.34 SVSISYY23 pKa = 9.17 PIVVHH28 pKa = 6.41 GVPGCGKK35 pKa = 10.42 SFICKK40 pKa = 9.58 EE41 pKa = 3.83 LAKK44 pKa = 10.61 DD45 pKa = 3.6 PKK47 pKa = 10.8 FKK49 pKa = 10.71 VQSFGVIPAGSLCGAVIHH67 pKa = 6.64 KK68 pKa = 10.5 ALTEE72 pKa = 3.89 PSGDD76 pKa = 3.44 FNVLDD81 pKa = 4.7 EE82 pKa = 4.34 YY83 pKa = 11.64 LGGKK87 pKa = 9.44 DD88 pKa = 3.67 FNLEE92 pKa = 3.84 RR93 pKa = 11.84 FDD95 pKa = 5.66 LVLSDD100 pKa = 4.87 PYY102 pKa = 11.32 QSFKK106 pKa = 10.88 KK107 pKa = 10.16 PEE109 pKa = 4.04 RR110 pKa = 11.84 AHH112 pKa = 5.27 FTSNITRR119 pKa = 11.84 RR120 pKa = 11.84 FGSQVCCLLNKK131 pKa = 10.28 LGFDD135 pKa = 4.66 IIPHH139 pKa = 6.6 PSLPAEE145 pKa = 3.84 QTLLIEE151 pKa = 4.63 GSLFKK156 pKa = 10.8 TEE158 pKa = 3.95 LRR160 pKa = 11.84 GIIITFEE167 pKa = 3.83 PAVAEE172 pKa = 4.74 LIRR175 pKa = 11.84 AHH177 pKa = 6.21 GLEE180 pKa = 4.62 PKK182 pKa = 10.1 HH183 pKa = 6.14 PCDD186 pKa = 3.3 VRR188 pKa = 11.84 GAEE191 pKa = 4.04 FEE193 pKa = 4.56 VVTFCTAHH201 pKa = 7.3 ADD203 pKa = 3.56 LQEE206 pKa = 4.3 IVSPEE211 pKa = 4.04 LYY213 pKa = 10.13 ISLTRR218 pKa = 11.84 ATKK221 pKa = 9.32 EE222 pKa = 4.17 LRR224 pKa = 11.84 VLTNKK229 pKa = 9.74 FDD231 pKa = 3.67 EE232 pKa = 4.62 SS233 pKa = 3.67
Molecular weight: 25.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.355
IPC2_protein 5.41
IPC_protein 5.372
Toseland 5.537
ProMoST 5.588
Dawson 5.474
Bjellqvist 5.55
Wikipedia 5.397
Rodwell 5.436
Grimsley 5.563
Solomon 5.474
Lehninger 5.448
Nozaki 5.664
DTASelect 5.804
Thurlkill 5.69
EMBOSS 5.626
Sillero 5.766
Patrickios 4.012
IPC_peptide 5.486
IPC2_peptide 5.766
IPC2.peptide.svr19 5.768
Protein with the highest isoelectric point:
>tr|C1BEG1|C1BEG1_9VIRU Movement protein TGBp3 OS=African oil palm ringspot virus OX=185218 PE=3 SV=1
MM1 pKa = 7.62 SLRR4 pKa = 11.84 PPTNYY9 pKa = 9.95 TNAILAASIGVAVGLLIHH27 pKa = 6.11 FVRR30 pKa = 11.84 RR31 pKa = 11.84 NEE33 pKa = 4.23 LPHH36 pKa = 7.97 SGDD39 pKa = 4.15 NIHH42 pKa = 6.65 HH43 pKa = 6.96 LPYY46 pKa = 10.24 GGQYY50 pKa = 9.59 MDD52 pKa = 3.25 GTKK55 pKa = 10.36 CINYY59 pKa = 8.94 NRR61 pKa = 11.84 GFGGRR66 pKa = 11.84 NFSLLGTSSNSGIWLLLLTTGLLLYY91 pKa = 7.51 VTRR94 pKa = 11.84 SCFARR99 pKa = 11.84 GGYY102 pKa = 9.26 IYY104 pKa = 10.45 HH105 pKa = 6.52 SNCHH109 pKa = 4.4 IHH111 pKa = 5.89 RR112 pKa = 11.84 TVV114 pKa = 2.64
Molecular weight: 12.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.543
IPC2_protein 9.531
IPC_protein 10.087
Toseland 9.268
ProMoST 9.414
Dawson 9.692
Bjellqvist 9.663
Wikipedia 9.999
Rodwell 9.677
Grimsley 9.838
Solomon 9.794
Lehninger 9.721
Nozaki 9.545
DTASelect 9.589
Thurlkill 9.502
EMBOSS 9.75
Sillero 9.692
Patrickios 2.422
IPC_peptide 9.765
IPC2_peptide 9.092
IPC2.peptide.svr19 8.211
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2528
69
1847
505.6
56.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.278 ± 0.987
2.492 ± 0.211
5.182 ± 1.029
6.646 ± 0.734
6.013 ± 0.696
5.538 ± 1.185
2.255 ± 0.633
5.815 ± 0.443
6.843 ± 1.036
9.296 ± 1.143
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.978 ± 0.399
4.074 ± 0.579
4.47 ± 0.358
2.769 ± 0.454
5.617 ± 0.262
9.059 ± 0.68
4.707 ± 1.215
6.092 ± 0.789
0.91 ± 0.246
2.888 ± 0.504
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here