Amino acid dipepetide frequency for Citrobacter phage SH3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.001AlaAla: 10.001 ± 1.088
0.887AlaCys: 0.887 ± 0.24
4.92AlaAsp: 4.92 ± 0.699
5.565AlaGlu: 5.565 ± 0.834
3.549AlaPhe: 3.549 ± 0.452
8.388AlaGly: 8.388 ± 0.898
0.968AlaHis: 0.968 ± 0.386
5.807AlaIle: 5.807 ± 0.724
6.291AlaLys: 6.291 ± 0.726
7.42AlaLeu: 7.42 ± 1.03
3.226AlaMet: 3.226 ± 0.572
3.549AlaAsn: 3.549 ± 0.414
2.581AlaPro: 2.581 ± 0.584
2.984AlaGln: 2.984 ± 0.498
3.549AlaArg: 3.549 ± 0.53
5.0AlaSer: 5.0 ± 0.716
4.355AlaThr: 4.355 ± 0.761
6.049AlaVal: 6.049 ± 0.876
1.371AlaTrp: 1.371 ± 0.357
2.5AlaTyr: 2.5 ± 0.528
0.0AlaXaa: 0.0 ± 0.0
Cys
0.968CysAla: 0.968 ± 0.257
0.081CysCys: 0.081 ± 0.095
0.807CysAsp: 0.807 ± 0.363
0.726CysGlu: 0.726 ± 0.241
0.645CysPhe: 0.645 ± 0.228
0.645CysGly: 0.645 ± 0.228
0.403CysHis: 0.403 ± 0.177
0.242CysIle: 0.242 ± 0.157
0.565CysLys: 0.565 ± 0.223
1.048CysLeu: 1.048 ± 0.291
0.323CysMet: 0.323 ± 0.22
0.484CysAsn: 0.484 ± 0.245
0.484CysPro: 0.484 ± 0.276
0.161CysGln: 0.161 ± 0.12
0.807CysArg: 0.807 ± 0.232
0.403CysSer: 0.403 ± 0.209
0.081CysThr: 0.081 ± 0.062
0.403CysVal: 0.403 ± 0.204
0.161CysTrp: 0.161 ± 0.13
0.081CysTyr: 0.081 ± 0.09
0.0CysXaa: 0.0 ± 0.0
Asp
6.371AspAla: 6.371 ± 0.827
0.645AspCys: 0.645 ± 0.298
4.033AspAsp: 4.033 ± 0.782
3.629AspGlu: 3.629 ± 0.504
1.936AspPhe: 1.936 ± 0.426
6.613AspGly: 6.613 ± 0.746
1.452AspHis: 1.452 ± 0.298
3.065AspIle: 3.065 ± 0.455
3.549AspLys: 3.549 ± 0.647
4.597AspLeu: 4.597 ± 0.568
2.339AspMet: 2.339 ± 0.396
2.742AspAsn: 2.742 ± 0.478
2.662AspPro: 2.662 ± 0.505
2.5AspGln: 2.5 ± 0.441
2.258AspArg: 2.258 ± 0.524
3.307AspSer: 3.307 ± 0.45
4.516AspThr: 4.516 ± 0.695
4.92AspVal: 4.92 ± 0.638
0.968AspTrp: 0.968 ± 0.387
1.936AspTyr: 1.936 ± 0.404
0.0AspXaa: 0.0 ± 0.0
Glu
6.291GluAla: 6.291 ± 1.108
0.645GluCys: 0.645 ± 0.252
5.242GluAsp: 5.242 ± 0.852
4.92GluGlu: 4.92 ± 0.7
2.097GluPhe: 2.097 ± 0.408
5.242GluGly: 5.242 ± 0.648
0.968GluHis: 0.968 ± 0.284
2.823GluIle: 2.823 ± 0.41
3.307GluLys: 3.307 ± 0.478
5.565GluLeu: 5.565 ± 0.741
2.258GluMet: 2.258 ± 0.495
2.178GluAsn: 2.178 ± 0.515
2.339GluPro: 2.339 ± 0.418
2.903GluGln: 2.903 ± 0.502
3.952GluArg: 3.952 ± 0.589
3.871GluSer: 3.871 ± 0.632
3.629GluThr: 3.629 ± 0.489
4.113GluVal: 4.113 ± 0.755
1.532GluTrp: 1.532 ± 0.295
2.903GluTyr: 2.903 ± 0.531
0.0GluXaa: 0.0 ± 0.0
Phe
3.226PheAla: 3.226 ± 0.623
0.242PheCys: 0.242 ± 0.171
3.145PheAsp: 3.145 ± 0.466
1.613PheGlu: 1.613 ± 0.32
1.048PhePhe: 1.048 ± 0.358
2.903PheGly: 2.903 ± 0.513
1.048PheHis: 1.048 ± 0.23
1.936PheIle: 1.936 ± 0.455
2.42PheLys: 2.42 ± 0.444
2.742PheLeu: 2.742 ± 0.442
0.887PheMet: 0.887 ± 0.268
2.097PheAsn: 2.097 ± 0.394
1.21PhePro: 1.21 ± 0.383
0.968PheGln: 0.968 ± 0.306
1.532PheArg: 1.532 ± 0.333
2.258PheSer: 2.258 ± 0.305
2.42PheThr: 2.42 ± 0.389
2.42PheVal: 2.42 ± 0.404
0.323PheTrp: 0.323 ± 0.137
1.048PheTyr: 1.048 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
6.21GlyAla: 6.21 ± 0.9
0.565GlyCys: 0.565 ± 0.261
4.92GlyAsp: 4.92 ± 0.928
5.484GlyGlu: 5.484 ± 0.74
1.774GlyPhe: 1.774 ± 0.3
5.323GlyGly: 5.323 ± 0.693
1.129GlyHis: 1.129 ± 0.311
3.871GlyIle: 3.871 ± 0.732
6.291GlyLys: 6.291 ± 0.73
5.888GlyLeu: 5.888 ± 0.702
2.581GlyMet: 2.581 ± 0.513
3.226GlyAsn: 3.226 ± 0.505
1.29GlyPro: 1.29 ± 0.302
2.984GlyGln: 2.984 ± 0.554
6.049GlyArg: 6.049 ± 0.782
6.21GlySer: 6.21 ± 0.73
4.194GlyThr: 4.194 ± 0.437
5.565GlyVal: 5.565 ± 0.706
1.29GlyTrp: 1.29 ± 0.34
3.629GlyTyr: 3.629 ± 0.586
0.0GlyXaa: 0.0 ± 0.0
His
0.968HisAla: 0.968 ± 0.303
0.242HisCys: 0.242 ± 0.13
1.21HisAsp: 1.21 ± 0.352
1.452HisGlu: 1.452 ± 0.42
0.323HisPhe: 0.323 ± 0.171
1.048HisGly: 1.048 ± 0.229
0.323HisHis: 0.323 ± 0.155
0.968HisIle: 0.968 ± 0.198
1.129HisLys: 1.129 ± 0.297
2.178HisLeu: 2.178 ± 0.491
0.807HisMet: 0.807 ± 0.244
0.484HisAsn: 0.484 ± 0.17
0.565HisPro: 0.565 ± 0.225
0.565HisGln: 0.565 ± 0.223
1.048HisArg: 1.048 ± 0.24
0.807HisSer: 0.807 ± 0.237
1.532HisThr: 1.532 ± 0.295
1.21HisVal: 1.21 ± 0.285
0.484HisTrp: 0.484 ± 0.21
0.645HisTyr: 0.645 ± 0.23
0.0HisXaa: 0.0 ± 0.0
Ile
3.145IleAla: 3.145 ± 0.519
0.726IleCys: 0.726 ± 0.297
3.387IleAsp: 3.387 ± 0.43
3.065IleGlu: 3.065 ± 0.463
1.129IlePhe: 1.129 ± 0.299
3.71IleGly: 3.71 ± 0.445
1.048IleHis: 1.048 ± 0.338
2.016IleIle: 2.016 ± 0.378
3.226IleLys: 3.226 ± 0.529
3.549IleLeu: 3.549 ± 0.525
0.968IleMet: 0.968 ± 0.309
2.903IleAsn: 2.903 ± 0.528
2.258IlePro: 2.258 ± 0.517
1.694IleGln: 1.694 ± 0.447
2.742IleArg: 2.742 ± 0.501
2.662IleSer: 2.662 ± 0.434
3.145IleThr: 3.145 ± 0.401
4.033IleVal: 4.033 ± 0.492
0.726IleTrp: 0.726 ± 0.22
1.452IleTyr: 1.452 ± 0.289
0.0IleXaa: 0.0 ± 0.0
Lys
6.855LysAla: 6.855 ± 0.754
0.887LysCys: 0.887 ± 0.305
3.952LysAsp: 3.952 ± 0.527
3.952LysGlu: 3.952 ± 0.622
2.662LysPhe: 2.662 ± 0.459
3.71LysGly: 3.71 ± 0.492
1.855LysHis: 1.855 ± 0.547
1.936LysIle: 1.936 ± 0.382
4.355LysLys: 4.355 ± 0.928
5.807LysLeu: 5.807 ± 0.637
1.855LysMet: 1.855 ± 0.302
2.258LysAsn: 2.258 ± 0.403
2.42LysPro: 2.42 ± 0.553
2.178LysGln: 2.178 ± 0.414
4.275LysArg: 4.275 ± 0.648
3.71LysSer: 3.71 ± 0.538
4.275LysThr: 4.275 ± 0.553
4.92LysVal: 4.92 ± 0.611
0.887LysTrp: 0.887 ± 0.282
2.258LysTyr: 2.258 ± 0.381
0.0LysXaa: 0.0 ± 0.0
Leu
6.855LeuAla: 6.855 ± 0.768
0.323LeuCys: 0.323 ± 0.213
4.678LeuAsp: 4.678 ± 0.456
7.017LeuGlu: 7.017 ± 0.899
2.42LeuPhe: 2.42 ± 0.415
4.355LeuGly: 4.355 ± 0.699
0.807LeuHis: 0.807 ± 0.207
3.226LeuIle: 3.226 ± 0.546
6.533LeuLys: 6.533 ± 0.838
5.404LeuLeu: 5.404 ± 0.685
2.742LeuMet: 2.742 ± 0.586
4.678LeuAsn: 4.678 ± 0.639
3.549LeuPro: 3.549 ± 0.39
4.275LeuGln: 4.275 ± 0.63
4.839LeuArg: 4.839 ± 0.413
5.081LeuSer: 5.081 ± 0.789
5.565LeuThr: 5.565 ± 0.67
5.162LeuVal: 5.162 ± 0.606
0.887LeuTrp: 0.887 ± 0.271
2.42LeuTyr: 2.42 ± 0.545
0.0LeuXaa: 0.0 ± 0.0
Met
3.629MetAla: 3.629 ± 0.406
0.484MetCys: 0.484 ± 0.234
1.855MetAsp: 1.855 ± 0.3
2.5MetGlu: 2.5 ± 0.396
1.371MetPhe: 1.371 ± 0.347
2.42MetGly: 2.42 ± 0.463
0.484MetHis: 0.484 ± 0.173
0.968MetIle: 0.968 ± 0.192
1.21MetLys: 1.21 ± 0.251
2.662MetLeu: 2.662 ± 0.445
0.645MetMet: 0.645 ± 0.242
0.968MetAsn: 0.968 ± 0.209
0.807MetPro: 0.807 ± 0.333
0.726MetGln: 0.726 ± 0.327
1.21MetArg: 1.21 ± 0.299
1.532MetSer: 1.532 ± 0.376
2.5MetThr: 2.5 ± 0.599
2.903MetVal: 2.903 ± 0.449
0.403MetTrp: 0.403 ± 0.27
1.129MetTyr: 1.129 ± 0.247
0.0MetXaa: 0.0 ± 0.0
Asn
4.436AsnAla: 4.436 ± 0.684
0.403AsnCys: 0.403 ± 0.193
2.016AsnAsp: 2.016 ± 0.475
2.339AsnGlu: 2.339 ± 0.486
1.532AsnPhe: 1.532 ± 0.229
4.597AsnGly: 4.597 ± 0.571
0.484AsnHis: 0.484 ± 0.181
2.178AsnIle: 2.178 ± 0.4
2.581AsnLys: 2.581 ± 0.376
3.629AsnLeu: 3.629 ± 0.617
1.371AsnMet: 1.371 ± 0.31
1.936AsnAsn: 1.936 ± 0.401
2.742AsnPro: 2.742 ± 0.626
1.774AsnGln: 1.774 ± 0.274
2.258AsnArg: 2.258 ± 0.586
2.178AsnSer: 2.178 ± 0.506
2.097AsnThr: 2.097 ± 0.432
2.823AsnVal: 2.823 ± 0.546
0.403AsnTrp: 0.403 ± 0.212
1.936AsnTyr: 1.936 ± 0.442
0.0AsnXaa: 0.0 ± 0.0
Pro
3.387ProAla: 3.387 ± 0.635
0.403ProCys: 0.403 ± 0.254
2.5ProAsp: 2.5 ± 0.348
2.984ProGlu: 2.984 ± 0.546
1.532ProPhe: 1.532 ± 0.298
1.855ProGly: 1.855 ± 0.457
0.565ProHis: 0.565 ± 0.202
2.016ProIle: 2.016 ± 0.373
2.662ProLys: 2.662 ± 0.519
2.42ProLeu: 2.42 ± 0.495
0.887ProMet: 0.887 ± 0.343
2.42ProAsn: 2.42 ± 0.557
1.29ProPro: 1.29 ± 0.369
1.613ProGln: 1.613 ± 0.393
1.694ProArg: 1.694 ± 0.428
2.5ProSer: 2.5 ± 0.429
2.903ProThr: 2.903 ± 0.485
2.742ProVal: 2.742 ± 0.368
0.807ProTrp: 0.807 ± 0.226
0.968ProTyr: 0.968 ± 0.27
0.0ProXaa: 0.0 ± 0.0
Gln
4.113GlnAla: 4.113 ± 0.511
0.242GlnCys: 0.242 ± 0.152
3.549GlnAsp: 3.549 ± 0.723
2.258GlnGlu: 2.258 ± 0.488
1.774GlnPhe: 1.774 ± 0.298
2.581GlnGly: 2.581 ± 0.396
0.726GlnHis: 0.726 ± 0.251
1.21GlnIle: 1.21 ± 0.332
1.774GlnLys: 1.774 ± 0.322
4.194GlnLeu: 4.194 ± 0.79
1.452GlnMet: 1.452 ± 0.386
1.774GlnAsn: 1.774 ± 0.406
1.048GlnPro: 1.048 ± 0.329
1.774GlnGln: 1.774 ± 0.598
2.258GlnArg: 2.258 ± 0.615
3.226GlnSer: 3.226 ± 0.414
1.936GlnThr: 1.936 ± 0.517
2.42GlnVal: 2.42 ± 0.378
0.645GlnTrp: 0.645 ± 0.264
1.21GlnTyr: 1.21 ± 0.368
0.0GlnXaa: 0.0 ± 0.0
Arg
4.436ArgAla: 4.436 ± 0.75
0.484ArgCys: 0.484 ± 0.165
4.033ArgAsp: 4.033 ± 0.462
3.791ArgGlu: 3.791 ± 0.487
2.662ArgPhe: 2.662 ± 0.355
4.113ArgGly: 4.113 ± 0.478
0.887ArgHis: 0.887 ± 0.289
3.307ArgIle: 3.307 ± 0.567
3.387ArgLys: 3.387 ± 0.575
5.484ArgLeu: 5.484 ± 0.678
1.048ArgMet: 1.048 ± 0.315
2.097ArgAsn: 2.097 ± 0.439
1.855ArgPro: 1.855 ± 0.303
2.662ArgGln: 2.662 ± 0.537
2.097ArgArg: 2.097 ± 0.37
2.984ArgSer: 2.984 ± 0.534
1.855ArgThr: 1.855 ± 0.36
3.065ArgVal: 3.065 ± 0.465
1.21ArgTrp: 1.21 ± 0.302
1.774ArgTyr: 1.774 ± 0.307
0.0ArgXaa: 0.0 ± 0.0
Ser
4.033SerAla: 4.033 ± 0.672
0.726SerCys: 0.726 ± 0.309
5.404SerAsp: 5.404 ± 0.494
2.662SerGlu: 2.662 ± 0.419
3.065SerPhe: 3.065 ± 0.43
5.726SerGly: 5.726 ± 0.717
1.855SerHis: 1.855 ± 0.381
2.823SerIle: 2.823 ± 0.65
3.71SerLys: 3.71 ± 0.483
3.871SerLeu: 3.871 ± 0.548
1.613SerMet: 1.613 ± 0.494
2.339SerAsn: 2.339 ± 0.603
2.662SerPro: 2.662 ± 0.496
2.258SerGln: 2.258 ± 0.41
2.984SerArg: 2.984 ± 0.494
3.952SerSer: 3.952 ± 0.568
2.984SerThr: 2.984 ± 0.39
4.033SerVal: 4.033 ± 0.502
0.807SerTrp: 0.807 ± 0.186
3.065SerTyr: 3.065 ± 0.498
0.0SerXaa: 0.0 ± 0.0
Thr
3.952ThrAla: 3.952 ± 0.663
0.242ThrCys: 0.242 ± 0.157
2.984ThrAsp: 2.984 ± 0.466
4.758ThrGlu: 4.758 ± 0.649
2.339ThrPhe: 2.339 ± 0.419
5.726ThrGly: 5.726 ± 0.511
0.726ThrHis: 0.726 ± 0.212
3.791ThrIle: 3.791 ± 0.592
3.307ThrLys: 3.307 ± 0.503
4.92ThrLeu: 4.92 ± 0.536
1.855ThrMet: 1.855 ± 0.395
2.016ThrAsn: 2.016 ± 0.476
3.307ThrPro: 3.307 ± 0.412
2.581ThrGln: 2.581 ± 0.509
2.742ThrArg: 2.742 ± 0.361
2.903ThrSer: 2.903 ± 0.643
3.549ThrThr: 3.549 ± 0.721
4.597ThrVal: 4.597 ± 0.644
0.484ThrTrp: 0.484 ± 0.206
1.613ThrTyr: 1.613 ± 0.359
0.0ThrXaa: 0.0 ± 0.0
Val
5.565ValAla: 5.565 ± 0.718
0.484ValCys: 0.484 ± 0.227
2.581ValAsp: 2.581 ± 0.422
5.404ValGlu: 5.404 ± 0.637
2.016ValPhe: 2.016 ± 0.54
5.404ValGly: 5.404 ± 0.625
1.129ValHis: 1.129 ± 0.465
3.307ValIle: 3.307 ± 0.568
5.242ValLys: 5.242 ± 0.555
4.92ValLeu: 4.92 ± 0.705
2.258ValMet: 2.258 ± 0.509
2.903ValAsn: 2.903 ± 0.519
3.307ValPro: 3.307 ± 0.583
3.307ValGln: 3.307 ± 0.528
4.194ValArg: 4.194 ± 0.602
5.242ValSer: 5.242 ± 0.671
4.194ValThr: 4.194 ± 0.526
6.371ValVal: 6.371 ± 0.925
0.726ValTrp: 0.726 ± 0.306
2.339ValTyr: 2.339 ± 0.363
0.0ValXaa: 0.0 ± 0.0
Trp
0.726TrpAla: 0.726 ± 0.206
0.403TrpCys: 0.403 ± 0.267
0.484TrpAsp: 0.484 ± 0.178
1.048TrpGlu: 1.048 ± 0.254
0.645TrpPhe: 0.645 ± 0.197
1.048TrpGly: 1.048 ± 0.32
0.484TrpHis: 0.484 ± 0.183
0.403TrpIle: 0.403 ± 0.195
1.452TrpLys: 1.452 ± 0.388
2.016TrpLeu: 2.016 ± 0.375
0.161TrpMet: 0.161 ± 0.101
1.21TrpAsn: 1.21 ± 0.302
0.403TrpPro: 0.403 ± 0.224
0.565TrpGln: 0.565 ± 0.241
0.726TrpArg: 0.726 ± 0.243
0.968TrpSer: 0.968 ± 0.442
0.645TrpThr: 0.645 ± 0.272
1.129TrpVal: 1.129 ± 0.382
0.161TrpTrp: 0.161 ± 0.102
0.403TrpTyr: 0.403 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.791TyrAla: 3.791 ± 0.617
0.403TyrCys: 0.403 ± 0.173
2.339TyrAsp: 2.339 ± 0.438
1.613TyrGlu: 1.613 ± 0.432
1.048TyrPhe: 1.048 ± 0.285
3.065TyrGly: 3.065 ± 0.605
0.645TyrHis: 0.645 ± 0.245
1.694TyrIle: 1.694 ± 0.455
2.016TyrLys: 2.016 ± 0.428
2.5TyrLeu: 2.5 ± 0.388
1.048TyrMet: 1.048 ± 0.302
1.452TyrAsn: 1.452 ± 0.366
1.371TyrPro: 1.371 ± 0.323
1.694TyrGln: 1.694 ± 0.529
2.016TyrArg: 2.016 ± 0.449
1.774TyrSer: 1.774 ± 0.336
2.016TyrThr: 2.016 ± 0.296
2.016TyrVal: 2.016 ± 0.451
0.807TyrTrp: 0.807 ± 0.222
1.452TyrTyr: 1.452 ± 0.295
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (12400 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski