Pacific flying fox faeces associated circular DNA virus-6
Average proteome isoelectric point is 8.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTU1|A0A140CTU1_9VIRU ATP-dependent helicase Rep OS=Pacific flying fox faeces associated circular DNA virus-6 OX=1796015 PE=3 SV=1
MM1 pKa = 7.46 SRR3 pKa = 11.84 DD4 pKa = 3.77 KK5 pKa = 11.47 VDD7 pKa = 4.01 LAPSRR12 pKa = 11.84 GFSKK16 pKa = 10.32 YY17 pKa = 6.88 WCFTAFASDD26 pKa = 3.75 FEE28 pKa = 4.67 KK29 pKa = 10.3 FTEE32 pKa = 4.77 IISDD36 pKa = 3.29 EE37 pKa = 4.29 RR38 pKa = 11.84 VFSNSDD44 pKa = 2.85 LSYY47 pKa = 10.57 IIGGEE52 pKa = 4.1 EE53 pKa = 4.13 LCPLSGRR60 pKa = 11.84 KK61 pKa = 9.13 HH62 pKa = 5.0 IQGFAAFASRR72 pKa = 11.84 KK73 pKa = 9.66 RR74 pKa = 11.84 LTACKK79 pKa = 10.12 KK80 pKa = 9.74 LFPSAHH86 pKa = 6.09 FEE88 pKa = 4.05 RR89 pKa = 11.84 MRR91 pKa = 11.84 GSARR95 pKa = 11.84 EE96 pKa = 3.95 AIEE99 pKa = 3.88 YY100 pKa = 9.34 CKK102 pKa = 10.52 KK103 pKa = 10.47 DD104 pKa = 3.17 GKK106 pKa = 10.5 FVEE109 pKa = 4.42 RR110 pKa = 11.84 GIYY113 pKa = 9.05 PCVRR117 pKa = 11.84 SSTVDD122 pKa = 2.9 NGSAKK127 pKa = 10.47 GVDD130 pKa = 3.54 RR131 pKa = 11.84 IKK133 pKa = 11.24 DD134 pKa = 4.08 FIEE137 pKa = 3.8 LARR140 pKa = 11.84 AGNVDD145 pKa = 3.81 LCEE148 pKa = 3.96 QRR150 pKa = 11.84 HH151 pKa = 5.55 PNQFLRR157 pKa = 11.84 YY158 pKa = 8.89 FSQLKK163 pKa = 9.76 NLRR166 pKa = 11.84 KK167 pKa = 9.42 FDD169 pKa = 3.64 IEE171 pKa = 4.18 RR172 pKa = 11.84 LSEE175 pKa = 4.06 ALWRR179 pKa = 11.84 LDD181 pKa = 3.38 LRR183 pKa = 11.84 GPSFGIGKK191 pKa = 9.35 DD192 pKa = 3.41 ASRR195 pKa = 11.84 SPSLRR200 pKa = 11.84 VFFFKK205 pKa = 10.61 KK206 pKa = 10.32 SQLKK210 pKa = 8.5 WWDD213 pKa = 3.49 GYY215 pKa = 11.3 NNEE218 pKa = 4.35 NIILISDD225 pKa = 4.15 FDD227 pKa = 4.66 EE228 pKa = 4.35 SCKK231 pKa = 10.62 WMVHH235 pKa = 5.09 YY236 pKa = 10.41 LKK238 pKa = 10.06 IWADD242 pKa = 3.3 VYY244 pKa = 11.05 PFNAEE249 pKa = 4.21 VKK251 pKa = 10.39 GSTIKK256 pKa = 10.19 IRR258 pKa = 11.84 PKK260 pKa = 10.61 YY261 pKa = 10.38 IIVTSNFRR269 pKa = 11.84 MDD271 pKa = 3.52 MLFLGTALDD280 pKa = 3.62 ALKK283 pKa = 10.52 RR284 pKa = 11.84 RR285 pKa = 11.84 FMTFEE290 pKa = 3.83 FSGSGLVEE298 pKa = 3.62 KK299 pKa = 10.04 QLRR302 pKa = 11.84 NEE304 pKa = 3.92 SHH306 pKa = 6.96 FNAFVLDD313 pKa = 3.77 ALKK316 pKa = 10.41 PYY318 pKa = 10.49 VDD320 pKa = 4.44 EE321 pKa = 5.98 DD322 pKa = 3.73 VDD324 pKa = 4.06 ASPSPSSSVLPNGGEE339 pKa = 3.61 ISPFTDD345 pKa = 3.7 EE346 pKa = 4.43 EE347 pKa = 4.86 TSTCLPSTSCAVDD360 pKa = 3.32 NPP362 pKa = 3.95
Molecular weight: 41.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.076
IPC2_protein 7.497
IPC_protein 7.366
Toseland 6.956
ProMoST 8.024
Dawson 8.083
Bjellqvist 8.463
Wikipedia 7.995
Rodwell 8.097
Grimsley 6.971
Solomon 8.156
Lehninger 8.185
Nozaki 8.726
DTASelect 8.185
Thurlkill 8.258
EMBOSS 8.302
Sillero 8.595
Patrickios 4.558
IPC_peptide 8.156
IPC2_peptide 7.424
IPC2.peptide.svr19 7.579
Protein with the highest isoelectric point:
>tr|A0A140CTU1|A0A140CTU1_9VIRU ATP-dependent helicase Rep OS=Pacific flying fox faeces associated circular DNA virus-6 OX=1796015 PE=3 SV=1
MM1 pKa = 7.6 WMRR4 pKa = 11.84 MWMRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 PFYY17 pKa = 9.89 RR18 pKa = 11.84 TAAKK22 pKa = 10.08 YY23 pKa = 10.04 RR24 pKa = 11.84 RR25 pKa = 11.84 LRR27 pKa = 11.84 MKK29 pKa = 9.79 KK30 pKa = 9.2 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 VYY35 pKa = 9.47 HH36 pKa = 6.28 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 VPLIIRR45 pKa = 11.84 EE46 pKa = 4.18 NYY48 pKa = 9.47 KK49 pKa = 9.02 KK50 pKa = 9.42 TIVRR54 pKa = 11.84 KK55 pKa = 8.21 VDD57 pKa = 2.83 QWNTHH62 pKa = 3.81 VWRR65 pKa = 11.84 GIRR68 pKa = 11.84 SVAPDD73 pKa = 2.85 NFMRR77 pKa = 11.84 FRR79 pKa = 11.84 DD80 pKa = 3.7 KK81 pKa = 10.94 LRR83 pKa = 11.84 CEE85 pKa = 4.66 MLTQDD90 pKa = 4.03 PQFISDD96 pKa = 3.76 LADD99 pKa = 3.16 YY100 pKa = 10.72 KK101 pKa = 10.9 YY102 pKa = 11.16 FKK104 pKa = 11.18 LNWFKK109 pKa = 11.38 LKK111 pKa = 10.14 IVSIAALEE119 pKa = 4.02 QQAMRR124 pKa = 11.84 VPPNSDD130 pKa = 2.84 PTLGFKK136 pKa = 10.49 SVIGHH141 pKa = 6.55 TGMNSLAWYY150 pKa = 9.84 VDD152 pKa = 2.71 WDD154 pKa = 4.28 LDD156 pKa = 4.12 HH157 pKa = 7.36 IAPKK161 pKa = 10.52 SPEE164 pKa = 4.03 DD165 pKa = 3.22 LRR167 pKa = 11.84 QRR169 pKa = 11.84 VSSYY173 pKa = 10.9 NMKK176 pKa = 10.41 KK177 pKa = 9.62 MYY179 pKa = 10.24 VGSFKK184 pKa = 10.68 PVTLFYY190 pKa = 10.81 RR191 pKa = 11.84 LPAEE195 pKa = 3.93 CRR197 pKa = 11.84 MYY199 pKa = 10.81 RR200 pKa = 11.84 ATALTASSGWKK211 pKa = 9.57 PSEE214 pKa = 4.07 STIGG218 pKa = 3.29
Molecular weight: 26.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.298
IPC2_protein 10.072
IPC_protein 11.052
Toseland 11.096
ProMoST 11.14
Dawson 11.155
Bjellqvist 10.979
Wikipedia 11.477
Rodwell 11.272
Grimsley 11.213
Solomon 11.418
Lehninger 11.359
Nozaki 11.082
DTASelect 10.979
Thurlkill 11.096
EMBOSS 11.535
Sillero 11.111
Patrickios 10.965
IPC_peptide 11.418
IPC2_peptide 10.175
IPC2.peptide.svr19 8.432
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
580
218
362
290.0
33.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.207 ± 0.14
1.897 ± 0.563
6.207 ± 0.668
5.0 ± 1.293
6.552 ± 1.394
4.828 ± 0.93
1.552 ± 0.163
5.172 ± 0.337
7.414 ± 0.221
7.586 ± 0.406
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.103 ± 1.381
3.621 ± 0.236
4.828 ± 0.389
2.069 ± 0.393
9.828 ± 2.527
8.966 ± 1.463
3.793 ± 0.457
5.345 ± 0.356
2.414 ± 0.722
3.621 ± 0.82
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here