Rhizoctonia mitovirus 1
Average proteome isoelectric point is 8.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A494RIS4|A0A494RIS4_9VIRU RNA-dependent RNA polymerase OS=Rhizoctonia mitovirus 1 OX=2421278 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.68 LFTTTTKK9 pKa = 10.95 AVISKK14 pKa = 6.57 TTTLFTRR21 pKa = 11.84 SIYY24 pKa = 10.02 TSHH27 pKa = 6.92 TLLNTTKK34 pKa = 9.98 TIEE37 pKa = 4.19 SLGLRR42 pKa = 11.84 GWQPMVPSVNEE53 pKa = 3.37 ARR55 pKa = 11.84 NNKK58 pKa = 9.61 RR59 pKa = 11.84 IANAISHH66 pKa = 6.69 LHH68 pKa = 6.24 LFMEE72 pKa = 4.82 TQTGLSNPAVMALTDD87 pKa = 5.73 LITEE91 pKa = 4.63 LRR93 pKa = 11.84 NEE95 pKa = 4.15 YY96 pKa = 10.57 NEE98 pKa = 4.35 NPLQFVRR105 pKa = 11.84 LSKK108 pKa = 11.16 LMGNWFKK115 pKa = 11.34 INIFQLKK122 pKa = 7.86 RR123 pKa = 11.84 TQGLIPVNMWDD134 pKa = 3.61 SYY136 pKa = 9.32 TNVPRR141 pKa = 11.84 GLVGIFSAFNQLSTDD156 pKa = 3.37 RR157 pKa = 11.84 EE158 pKa = 3.96 RR159 pKa = 11.84 IIFIRR164 pKa = 11.84 MVLSILDD171 pKa = 4.01 LYY173 pKa = 10.75 RR174 pKa = 11.84 VVVTPTKK181 pKa = 9.87 PDD183 pKa = 2.93 LGTIVNPGTDD193 pKa = 2.44 IGGIIKK199 pKa = 10.31 DD200 pKa = 4.01 DD201 pKa = 4.76 EE202 pKa = 4.39 IQKK205 pKa = 10.1 SLEE208 pKa = 3.92 MLGIDD213 pKa = 5.44 CDD215 pKa = 4.12 LLTNNFKK222 pKa = 10.46 FANKK226 pKa = 9.98 AVFFHH231 pKa = 6.87 SSSAQGPNGQAVWNSHH247 pKa = 6.04 LDD249 pKa = 3.69 AKK251 pKa = 11.1 ALLADD256 pKa = 3.99 KK257 pKa = 10.98 DD258 pKa = 3.87 LFEE261 pKa = 5.23 NVQQLALLLEE271 pKa = 4.7 RR272 pKa = 11.84 KK273 pKa = 9.88 DD274 pKa = 3.64 LLTVLEE280 pKa = 4.65 EE281 pKa = 4.05 AAKK284 pKa = 10.76 LPDD287 pKa = 4.63 LISTNEE293 pKa = 3.85 EE294 pKa = 3.86 PLKK297 pKa = 10.57 HH298 pKa = 6.35 SKK300 pKa = 10.26 LHH302 pKa = 6.82 IIFEE306 pKa = 4.7 NGDD309 pKa = 3.29 KK310 pKa = 10.52 VRR312 pKa = 11.84 PVAIIDD318 pKa = 3.87 YY319 pKa = 7.47 FTQEE323 pKa = 4.36 LLSPFHH329 pKa = 7.06 DD330 pKa = 4.01 LVAGILRR337 pKa = 11.84 SIPQDD342 pKa = 3.02 GTFNQNAIASKK353 pKa = 10.19 VKK355 pKa = 10.46 EE356 pKa = 4.34 FTATAGNSLFSFDD369 pKa = 3.86 LTAATDD375 pKa = 3.88 RR376 pKa = 11.84 LPVILQRR383 pKa = 11.84 RR384 pKa = 11.84 IISHH388 pKa = 7.02 IIKK391 pKa = 9.6 IDD393 pKa = 3.31 RR394 pKa = 11.84 FALLWQKK401 pKa = 10.79 VLTFRR406 pKa = 11.84 DD407 pKa = 4.43 FSLGNGHH414 pKa = 5.86 SVRR417 pKa = 11.84 YY418 pKa = 9.36 AVGQPMGAKK427 pKa = 9.91 SSFPMLGLTHH437 pKa = 6.91 HH438 pKa = 7.24 IIVQIAALRR447 pKa = 11.84 VGFSTMFKK455 pKa = 10.46 DD456 pKa = 4.32 YY457 pKa = 11.23 VILGDD462 pKa = 5.23 DD463 pKa = 3.65 IMIANEE469 pKa = 4.0 KK470 pKa = 10.36 VATQYY475 pKa = 10.93 RR476 pKa = 11.84 RR477 pKa = 11.84 IMEE480 pKa = 4.31 SLGLAISQHH489 pKa = 5.83 KK490 pKa = 10.43 SIISTNSTTSAQIAEE505 pKa = 3.84 ICRR508 pKa = 11.84 RR509 pKa = 11.84 VFVRR513 pKa = 11.84 GIEE516 pKa = 4.01 ITPLPMKK523 pKa = 10.69 LIANVFEE530 pKa = 4.51 NGSMFLQLQEE540 pKa = 4.32 KK541 pKa = 8.53 LTEE544 pKa = 4.58 RR545 pKa = 11.84 GLVFAPEE552 pKa = 3.9 NFEE555 pKa = 4.56 LLVAGFKK562 pKa = 10.89 LSLQDD567 pKa = 3.4 FNQVVLQNSLPQWLTKK583 pKa = 9.67 LTEE586 pKa = 4.17 TFSFEE591 pKa = 6.65 DD592 pKa = 3.27 SDD594 pKa = 3.45 EE595 pKa = 4.44 WNFKK599 pKa = 8.13 TWTDD603 pKa = 3.49 VGIDD607 pKa = 3.58 EE608 pKa = 5.09 QMLLEE613 pKa = 4.36 FWKK616 pKa = 9.01 YY617 pKa = 8.02 TVISEE622 pKa = 4.03 QFKK625 pKa = 10.82 RR626 pKa = 11.84 ISTLIQAATNGFKK639 pKa = 10.61 AISKK643 pKa = 10.16 AISLGDD649 pKa = 3.31 ILFAQDD655 pKa = 3.75 PQTGDD660 pKa = 3.87 RR661 pKa = 11.84 IPAKK665 pKa = 9.94 MFTLEE670 pKa = 5.5 TLQQWNILKK679 pKa = 9.72 VAHH682 pKa = 6.89 PARR685 pKa = 11.84 FVMLKK690 pKa = 9.23 EE691 pKa = 3.99 VEE693 pKa = 4.36 RR694 pKa = 11.84 ISGIMRR700 pKa = 11.84 RR701 pKa = 11.84 VASARR706 pKa = 11.84 GAEE709 pKa = 4.26 LFVSLMDD716 pKa = 5.71 SIVDD720 pKa = 3.43 QLKK723 pKa = 9.98 YY724 pKa = 10.97 SILEE728 pKa = 4.21 VIKK731 pKa = 11.07 DD732 pKa = 3.62 KK733 pKa = 11.04 DD734 pKa = 3.73 LKK736 pKa = 10.48 ASQMTRR742 pKa = 11.84 SMIDD746 pKa = 2.8 KK747 pKa = 9.31 TLANMRR753 pKa = 11.84 AANLAEE759 pKa = 4.07 NKK761 pKa = 9.72 QLSFTVKK768 pKa = 10.46 LEE770 pKa = 3.93 SLSIVWVLKK779 pKa = 10.06 VSLNGSCTLSEE790 pKa = 4.14 STMNVPTTTRR800 pKa = 11.84 DD801 pKa = 3.22 AKK803 pKa = 11.0 AQLLKK808 pKa = 10.84 YY809 pKa = 10.39 RR810 pKa = 11.84 SQNQSFKK817 pKa = 11.34 SSFGSSKK824 pKa = 9.73 STT826 pKa = 3.32
Molecular weight: 92.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.558
IPC2_protein 8.375
IPC_protein 8.273
Toseland 9.224
ProMoST 8.873
Dawson 9.443
Bjellqvist 9.121
Wikipedia 9.56
Rodwell 9.853
Grimsley 9.472
Solomon 9.487
Lehninger 9.458
Nozaki 9.311
DTASelect 9.077
Thurlkill 9.311
EMBOSS 9.648
Sillero 9.399
Patrickios 5.486
IPC_peptide 9.487
IPC2_peptide 7.571
IPC2.peptide.svr19 7.71
Protein with the highest isoelectric point:
>tr|A0A494RIS4|A0A494RIS4_9VIRU RNA-dependent RNA polymerase OS=Rhizoctonia mitovirus 1 OX=2421278 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.68 LFTTTTKK9 pKa = 10.95 AVISKK14 pKa = 6.57 TTTLFTRR21 pKa = 11.84 SIYY24 pKa = 10.02 TSHH27 pKa = 6.92 TLLNTTKK34 pKa = 9.98 TIEE37 pKa = 4.19 SLGLRR42 pKa = 11.84 GWQPMVPSVNEE53 pKa = 3.37 ARR55 pKa = 11.84 NNKK58 pKa = 9.61 RR59 pKa = 11.84 IANAISHH66 pKa = 6.69 LHH68 pKa = 6.24 LFMEE72 pKa = 4.82 TQTGLSNPAVMALTDD87 pKa = 5.73 LITEE91 pKa = 4.63 LRR93 pKa = 11.84 NEE95 pKa = 4.15 YY96 pKa = 10.57 NEE98 pKa = 4.35 NPLQFVRR105 pKa = 11.84 LSKK108 pKa = 11.16 LMGNWFKK115 pKa = 11.34 INIFQLKK122 pKa = 7.86 RR123 pKa = 11.84 TQGLIPVNMWDD134 pKa = 3.61 SYY136 pKa = 9.32 TNVPRR141 pKa = 11.84 GLVGIFSAFNQLSTDD156 pKa = 3.37 RR157 pKa = 11.84 EE158 pKa = 3.96 RR159 pKa = 11.84 IIFIRR164 pKa = 11.84 MVLSILDD171 pKa = 4.01 LYY173 pKa = 10.75 RR174 pKa = 11.84 VVVTPTKK181 pKa = 9.87 PDD183 pKa = 2.93 LGTIVNPGTDD193 pKa = 2.44 IGGIIKK199 pKa = 10.31 DD200 pKa = 4.01 DD201 pKa = 4.76 EE202 pKa = 4.39 IQKK205 pKa = 10.1 SLEE208 pKa = 3.92 MLGIDD213 pKa = 5.44 CDD215 pKa = 4.12 LLTNNFKK222 pKa = 10.46 FANKK226 pKa = 9.98 AVFFHH231 pKa = 6.87 SSSAQGPNGQAVWNSHH247 pKa = 6.04 LDD249 pKa = 3.69 AKK251 pKa = 11.1 ALLADD256 pKa = 3.99 KK257 pKa = 10.98 DD258 pKa = 3.87 LFEE261 pKa = 5.23 NVQQLALLLEE271 pKa = 4.7 RR272 pKa = 11.84 KK273 pKa = 9.88 DD274 pKa = 3.64 LLTVLEE280 pKa = 4.65 EE281 pKa = 4.05 AAKK284 pKa = 10.76 LPDD287 pKa = 4.63 LISTNEE293 pKa = 3.85 EE294 pKa = 3.86 PLKK297 pKa = 10.57 HH298 pKa = 6.35 SKK300 pKa = 10.26 LHH302 pKa = 6.82 IIFEE306 pKa = 4.7 NGDD309 pKa = 3.29 KK310 pKa = 10.52 VRR312 pKa = 11.84 PVAIIDD318 pKa = 3.87 YY319 pKa = 7.47 FTQEE323 pKa = 4.36 LLSPFHH329 pKa = 7.06 DD330 pKa = 4.01 LVAGILRR337 pKa = 11.84 SIPQDD342 pKa = 3.02 GTFNQNAIASKK353 pKa = 10.19 VKK355 pKa = 10.46 EE356 pKa = 4.34 FTATAGNSLFSFDD369 pKa = 3.86 LTAATDD375 pKa = 3.88 RR376 pKa = 11.84 LPVILQRR383 pKa = 11.84 RR384 pKa = 11.84 IISHH388 pKa = 7.02 IIKK391 pKa = 9.6 IDD393 pKa = 3.31 RR394 pKa = 11.84 FALLWQKK401 pKa = 10.79 VLTFRR406 pKa = 11.84 DD407 pKa = 4.43 FSLGNGHH414 pKa = 5.86 SVRR417 pKa = 11.84 YY418 pKa = 9.36 AVGQPMGAKK427 pKa = 9.91 SSFPMLGLTHH437 pKa = 6.91 HH438 pKa = 7.24 IIVQIAALRR447 pKa = 11.84 VGFSTMFKK455 pKa = 10.46 DD456 pKa = 4.32 YY457 pKa = 11.23 VILGDD462 pKa = 5.23 DD463 pKa = 3.65 IMIANEE469 pKa = 4.0 KK470 pKa = 10.36 VATQYY475 pKa = 10.93 RR476 pKa = 11.84 RR477 pKa = 11.84 IMEE480 pKa = 4.31 SLGLAISQHH489 pKa = 5.83 KK490 pKa = 10.43 SIISTNSTTSAQIAEE505 pKa = 3.84 ICRR508 pKa = 11.84 RR509 pKa = 11.84 VFVRR513 pKa = 11.84 GIEE516 pKa = 4.01 ITPLPMKK523 pKa = 10.69 LIANVFEE530 pKa = 4.51 NGSMFLQLQEE540 pKa = 4.32 KK541 pKa = 8.53 LTEE544 pKa = 4.58 RR545 pKa = 11.84 GLVFAPEE552 pKa = 3.9 NFEE555 pKa = 4.56 LLVAGFKK562 pKa = 10.89 LSLQDD567 pKa = 3.4 FNQVVLQNSLPQWLTKK583 pKa = 9.67 LTEE586 pKa = 4.17 TFSFEE591 pKa = 6.65 DD592 pKa = 3.27 SDD594 pKa = 3.45 EE595 pKa = 4.44 WNFKK599 pKa = 8.13 TWTDD603 pKa = 3.49 VGIDD607 pKa = 3.58 EE608 pKa = 5.09 QMLLEE613 pKa = 4.36 FWKK616 pKa = 9.01 YY617 pKa = 8.02 TVISEE622 pKa = 4.03 QFKK625 pKa = 10.82 RR626 pKa = 11.84 ISTLIQAATNGFKK639 pKa = 10.61 AISKK643 pKa = 10.16 AISLGDD649 pKa = 3.31 ILFAQDD655 pKa = 3.75 PQTGDD660 pKa = 3.87 RR661 pKa = 11.84 IPAKK665 pKa = 9.94 MFTLEE670 pKa = 5.5 TLQQWNILKK679 pKa = 9.72 VAHH682 pKa = 6.89 PARR685 pKa = 11.84 FVMLKK690 pKa = 9.23 EE691 pKa = 3.99 VEE693 pKa = 4.36 RR694 pKa = 11.84 ISGIMRR700 pKa = 11.84 RR701 pKa = 11.84 VASARR706 pKa = 11.84 GAEE709 pKa = 4.26 LFVSLMDD716 pKa = 5.71 SIVDD720 pKa = 3.43 QLKK723 pKa = 9.98 YY724 pKa = 10.97 SILEE728 pKa = 4.21 VIKK731 pKa = 11.07 DD732 pKa = 3.62 KK733 pKa = 11.04 DD734 pKa = 3.73 LKK736 pKa = 10.48 ASQMTRR742 pKa = 11.84 SMIDD746 pKa = 2.8 KK747 pKa = 9.31 TLANMRR753 pKa = 11.84 AANLAEE759 pKa = 4.07 NKK761 pKa = 9.72 QLSFTVKK768 pKa = 10.46 LEE770 pKa = 3.93 SLSIVWVLKK779 pKa = 10.06 VSLNGSCTLSEE790 pKa = 4.14 STMNVPTTTRR800 pKa = 11.84 DD801 pKa = 3.22 AKK803 pKa = 11.0 AQLLKK808 pKa = 10.84 YY809 pKa = 10.39 RR810 pKa = 11.84 SQNQSFKK817 pKa = 11.34 SSFGSSKK824 pKa = 9.73 STT826 pKa = 3.32
Molecular weight: 92.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.558
IPC2_protein 8.375
IPC_protein 8.273
Toseland 9.224
ProMoST 8.873
Dawson 9.443
Bjellqvist 9.121
Wikipedia 9.56
Rodwell 9.853
Grimsley 9.472
Solomon 9.487
Lehninger 9.458
Nozaki 9.311
DTASelect 9.077
Thurlkill 9.311
EMBOSS 9.648
Sillero 9.399
Patrickios 5.486
IPC_peptide 9.487
IPC2_peptide 7.571
IPC2.peptide.svr19 7.71
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
826
826
826
826.0
92.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.78 ± 0.0
0.363 ± 0.0
4.843 ± 0.0
4.964 ± 0.0
5.69 ± 0.0
4.6 ± 0.0
1.695 ± 0.0
7.869 ± 0.0
6.295 ± 0.0
11.864 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.906 ± 0.0
5.206 ± 0.0
3.148 ± 0.0
4.843 ± 0.0
4.722 ± 0.0
7.99 ± 0.0
7.506 ± 0.0
6.053 ± 0.0
1.332 ± 0.0
1.332 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here