Synechococcus phage S-SCSM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 292 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M2ZHX6|A0A6M2ZHX6_9CAUD Uncharacterized protein OS=Synechococcus phage S-SCSM1 OX=2588487 GN=SSCSM1_127 PE=4 SV=1
MM1 pKa = 7.8NYY3 pKa = 8.67TLKK6 pKa = 10.56QLQDD10 pKa = 3.15KK11 pKa = 9.16LNRR14 pKa = 11.84MIADD18 pKa = 3.39QGEE21 pKa = 4.22DD22 pKa = 3.55AQCAAWIYY30 pKa = 9.38TKK32 pKa = 10.9EE33 pKa = 4.15DD34 pKa = 3.12IYY36 pKa = 11.31RR37 pKa = 11.84VDD39 pKa = 3.47EE40 pKa = 5.04DD41 pKa = 5.08GEE43 pKa = 4.09FDD45 pKa = 5.48YY46 pKa = 11.04FALDD50 pKa = 3.38NPEE53 pKa = 3.75LVEE56 pKa = 5.13RR57 pKa = 11.84VFDD60 pKa = 3.98DD61 pKa = 3.71VGNIDD66 pKa = 4.42YY67 pKa = 9.94IYY69 pKa = 10.71QVIQEE74 pKa = 4.7CVDD77 pKa = 3.65EE78 pKa = 4.38VAEE81 pKa = 4.16EE82 pKa = 4.13QFVQQQQEE90 pKa = 4.28LVV92 pKa = 3.44

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M2ZHU2|A0A6M2ZHU2_9CAUD Uncharacterized protein OS=Synechococcus phage S-SCSM1 OX=2588487 GN=SSCSM1_226 PE=4 SV=1
MM1 pKa = 7.61TKK3 pKa = 9.62TKK5 pKa = 10.53LRR7 pKa = 11.84AQVKK11 pKa = 9.33SRR13 pKa = 11.84FYY15 pKa = 10.24YY16 pKa = 9.41VFWGTATLAVVLGQLYY32 pKa = 10.21VGTGYY37 pKa = 10.73RR38 pKa = 11.84VLAGRR43 pKa = 11.84MDD45 pKa = 3.64QLLQKK50 pKa = 10.44VDD52 pKa = 3.54GVLLYY57 pKa = 10.69KK58 pKa = 10.56SNKK61 pKa = 8.9PGLFF65 pKa = 3.49

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

292

0

292

73290

28

4120

251.0

28.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.744 ± 0.211

1.098 ± 0.087

6.749 ± 0.114

6.518 ± 0.248

4.507 ± 0.135

7.63 ± 0.283

1.907 ± 0.124

6.485 ± 0.174

6.064 ± 0.283

7.307 ± 0.144

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.055 ± 0.131

6.012 ± 0.147

4.04 ± 0.155

3.369 ± 0.105

4.073 ± 0.133

7.147 ± 0.191

6.829 ± 0.261

6.71 ± 0.154

1.223 ± 0.09

4.53 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski