Circoviridae 9 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 7.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S5T685|S5T685_9CIRC Uncharacterized protein OS=Circoviridae 9 LDMD-2013 OX=1379713 PE=4 SV=1
MM1 pKa = 7.42SGSLSGTTLYY11 pKa = 10.88LLYY14 pKa = 10.23MNKK17 pKa = 8.98QDD19 pKa = 3.58KK20 pKa = 10.58HH21 pKa = 4.62WVFTLNNYY29 pKa = 7.56TEE31 pKa = 4.34EE32 pKa = 4.4EE33 pKa = 4.4YY34 pKa = 11.01EE35 pKa = 4.11QLVEE39 pKa = 4.03LAEE42 pKa = 4.48SEE44 pKa = 4.31DD45 pKa = 3.44VSYY48 pKa = 11.2AIYY51 pKa = 10.22GKK53 pKa = 8.48EE54 pKa = 4.16TGDD57 pKa = 3.27SGTPHH62 pKa = 5.48IQGYY66 pKa = 9.86IRR68 pKa = 11.84FARR71 pKa = 11.84KK72 pKa = 9.29KK73 pKa = 9.12RR74 pKa = 11.84FNGVRR79 pKa = 11.84RR80 pKa = 11.84ILGKK84 pKa = 9.96RR85 pKa = 11.84AHH87 pKa = 6.62IEE89 pKa = 4.3CKK91 pKa = 10.34KK92 pKa = 9.66GTPTQASDD100 pKa = 3.49YY101 pKa = 9.76CKK103 pKa = 10.5KK104 pKa = 10.86DD105 pKa = 2.56GDD107 pKa = 4.04YY108 pKa = 11.46QEE110 pKa = 4.8FGCRR114 pKa = 11.84ATKK117 pKa = 9.76PGRR120 pKa = 11.84RR121 pKa = 11.84TDD123 pKa = 3.87LEE125 pKa = 4.0EE126 pKa = 5.9LYY128 pKa = 11.03LQVKK132 pKa = 8.82EE133 pKa = 4.22GKK135 pKa = 8.05TRR137 pKa = 11.84DD138 pKa = 3.73EE139 pKa = 4.49IGDD142 pKa = 4.07TFTGSYY148 pKa = 9.77IRR150 pKa = 11.84YY151 pKa = 8.8KK152 pKa = 10.69RR153 pKa = 11.84SIDD156 pKa = 3.57EE157 pKa = 5.14LIKK160 pKa = 10.24QHH162 pKa = 6.85HH163 pKa = 6.22DD164 pKa = 3.15TPRR167 pKa = 11.84TRR169 pKa = 11.84TCTAPTVIVHH179 pKa = 6.74WGLTGTGKK187 pKa = 8.01TRR189 pKa = 11.84AVWDD193 pKa = 3.8EE194 pKa = 4.08HH195 pKa = 6.64WDD197 pKa = 3.44DD198 pKa = 5.16LYY200 pKa = 11.9AHH202 pKa = 7.09GGDD205 pKa = 3.17RR206 pKa = 11.84WLTDD210 pKa = 3.01TMDD213 pKa = 3.68NEE215 pKa = 4.65LFYY218 pKa = 10.85STTSMGANLNSPTFSGCC235 pKa = 3.46

Molecular weight:
26.96 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S5TMW2|S5TMW2_9CIRC Uncharacterized protein OS=Circoviridae 9 LDMD-2013 OX=1379713 PE=4 SV=1
MM1 pKa = 7.79AYY3 pKa = 10.31YY4 pKa = 9.32WIPPKK9 pKa = 10.42YY10 pKa = 9.91RR11 pKa = 11.84SFEE14 pKa = 4.03KK15 pKa = 10.67YY16 pKa = 9.99LPSSARR22 pKa = 11.84TFINRR27 pKa = 11.84GEE29 pKa = 4.87DD30 pKa = 3.02INDD33 pKa = 4.29RR34 pKa = 11.84IGSSLHH40 pKa = 5.21QAAKK44 pKa = 9.87FAWDD48 pKa = 3.72TYY50 pKa = 11.09VNSLPQKK57 pKa = 10.52GGAQVITPKK66 pKa = 10.32SSSYY70 pKa = 9.21TRR72 pKa = 11.84SGAKK76 pKa = 9.53YY77 pKa = 9.87LPPRR81 pKa = 11.84TPSKK85 pKa = 10.32KK86 pKa = 9.8RR87 pKa = 11.84KK88 pKa = 9.26ASSKK92 pKa = 10.59RR93 pKa = 11.84DD94 pKa = 3.67TLPKK98 pKa = 9.41TRR100 pKa = 11.84RR101 pKa = 11.84LTFSPKK107 pKa = 10.22KK108 pKa = 10.13SIKK111 pKa = 9.77PKK113 pKa = 9.63PDD115 pKa = 3.46PPKK118 pKa = 10.02MPRR121 pKa = 11.84TRR123 pKa = 11.84KK124 pKa = 8.67GRR126 pKa = 11.84SYY128 pKa = 9.06STIGAGHH135 pKa = 6.19YY136 pKa = 9.45KK137 pKa = 10.68GKK139 pKa = 10.42FGSGKK144 pKa = 9.21FRR146 pKa = 11.84KK147 pKa = 7.8TPRR150 pKa = 11.84RR151 pKa = 3.55

Molecular weight:
17.2 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

704

151

318

234.7

26.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.102 ± 1.39

1.42 ± 0.423

5.54 ± 0.774

4.403 ± 1.201

3.409 ± 0.196

8.523 ± 0.406

1.847 ± 0.616

5.682 ± 0.794

8.239 ± 1.616

6.25 ± 0.662

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.42 ± 0.111

4.119 ± 0.964

4.545 ± 1.724

2.841 ± 0.248

6.108 ± 1.537

8.949 ± 1.374

8.097 ± 1.183

4.688 ± 1.358

1.847 ± 0.158

4.972 ± 0.61

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski