Circoviridae 9 LDMD-2013
Average proteome isoelectric point is 7.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5T685|S5T685_9CIRC Uncharacterized protein OS=Circoviridae 9 LDMD-2013 OX=1379713 PE=4 SV=1
MM1 pKa = 7.42 SGSLSGTTLYY11 pKa = 10.88 LLYY14 pKa = 10.23 MNKK17 pKa = 8.98 QDD19 pKa = 3.58 KK20 pKa = 10.58 HH21 pKa = 4.62 WVFTLNNYY29 pKa = 7.56 TEE31 pKa = 4.34 EE32 pKa = 4.4 EE33 pKa = 4.4 YY34 pKa = 11.01 EE35 pKa = 4.11 QLVEE39 pKa = 4.03 LAEE42 pKa = 4.48 SEE44 pKa = 4.31 DD45 pKa = 3.44 VSYY48 pKa = 11.2 AIYY51 pKa = 10.22 GKK53 pKa = 8.48 EE54 pKa = 4.16 TGDD57 pKa = 3.27 SGTPHH62 pKa = 5.48 IQGYY66 pKa = 9.86 IRR68 pKa = 11.84 FARR71 pKa = 11.84 KK72 pKa = 9.29 KK73 pKa = 9.12 RR74 pKa = 11.84 FNGVRR79 pKa = 11.84 RR80 pKa = 11.84 ILGKK84 pKa = 9.96 RR85 pKa = 11.84 AHH87 pKa = 6.62 IEE89 pKa = 4.3 CKK91 pKa = 10.34 KK92 pKa = 9.66 GTPTQASDD100 pKa = 3.49 YY101 pKa = 9.76 CKK103 pKa = 10.5 KK104 pKa = 10.86 DD105 pKa = 2.56 GDD107 pKa = 4.04 YY108 pKa = 11.46 QEE110 pKa = 4.8 FGCRR114 pKa = 11.84 ATKK117 pKa = 9.76 PGRR120 pKa = 11.84 RR121 pKa = 11.84 TDD123 pKa = 3.87 LEE125 pKa = 4.0 EE126 pKa = 5.9 LYY128 pKa = 11.03 LQVKK132 pKa = 8.82 EE133 pKa = 4.22 GKK135 pKa = 8.05 TRR137 pKa = 11.84 DD138 pKa = 3.73 EE139 pKa = 4.49 IGDD142 pKa = 4.07 TFTGSYY148 pKa = 9.77 IRR150 pKa = 11.84 YY151 pKa = 8.8 KK152 pKa = 10.69 RR153 pKa = 11.84 SIDD156 pKa = 3.57 EE157 pKa = 5.14 LIKK160 pKa = 10.24 QHH162 pKa = 6.85 HH163 pKa = 6.22 DD164 pKa = 3.15 TPRR167 pKa = 11.84 TRR169 pKa = 11.84 TCTAPTVIVHH179 pKa = 6.74 WGLTGTGKK187 pKa = 8.01 TRR189 pKa = 11.84 AVWDD193 pKa = 3.8 EE194 pKa = 4.08 HH195 pKa = 6.64 WDD197 pKa = 3.44 DD198 pKa = 5.16 LYY200 pKa = 11.9 AHH202 pKa = 7.09 GGDD205 pKa = 3.17 RR206 pKa = 11.84 WLTDD210 pKa = 3.01 TMDD213 pKa = 3.68 NEE215 pKa = 4.65 LFYY218 pKa = 10.85 STTSMGANLNSPTFSGCC235 pKa = 3.46
Molecular weight: 26.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.056
IPC2_protein 6.059
IPC_protein 6.16
Toseland 6.478
ProMoST 6.561
Dawson 6.491
Bjellqvist 6.453
Wikipedia 6.517
Rodwell 6.478
Grimsley 6.605
Solomon 6.491
Lehninger 6.491
Nozaki 6.751
DTASelect 6.942
Thurlkill 6.956
EMBOSS 6.942
Sillero 6.883
Patrickios 4.024
IPC_peptide 6.517
IPC2_peptide 6.751
IPC2.peptide.svr19 6.738
Protein with the highest isoelectric point:
>tr|S5TMW2|S5TMW2_9CIRC Uncharacterized protein OS=Circoviridae 9 LDMD-2013 OX=1379713 PE=4 SV=1
MM1 pKa = 7.79 AYY3 pKa = 10.31 YY4 pKa = 9.32 WIPPKK9 pKa = 10.42 YY10 pKa = 9.91 RR11 pKa = 11.84 SFEE14 pKa = 4.03 KK15 pKa = 10.67 YY16 pKa = 9.99 LPSSARR22 pKa = 11.84 TFINRR27 pKa = 11.84 GEE29 pKa = 4.87 DD30 pKa = 3.02 INDD33 pKa = 4.29 RR34 pKa = 11.84 IGSSLHH40 pKa = 5.21 QAAKK44 pKa = 9.87 FAWDD48 pKa = 3.72 TYY50 pKa = 11.09 VNSLPQKK57 pKa = 10.52 GGAQVITPKK66 pKa = 10.32 SSSYY70 pKa = 9.21 TRR72 pKa = 11.84 SGAKK76 pKa = 9.53 YY77 pKa = 9.87 LPPRR81 pKa = 11.84 TPSKK85 pKa = 10.32 KK86 pKa = 9.8 RR87 pKa = 11.84 KK88 pKa = 9.26 ASSKK92 pKa = 10.59 RR93 pKa = 11.84 DD94 pKa = 3.67 TLPKK98 pKa = 9.41 TRR100 pKa = 11.84 RR101 pKa = 11.84 LTFSPKK107 pKa = 10.22 KK108 pKa = 10.13 SIKK111 pKa = 9.77 PKK113 pKa = 9.63 PDD115 pKa = 3.46 PPKK118 pKa = 10.02 MPRR121 pKa = 11.84 TRR123 pKa = 11.84 KK124 pKa = 8.67 GRR126 pKa = 11.84 SYY128 pKa = 9.06 STIGAGHH135 pKa = 6.19 YY136 pKa = 9.45 KK137 pKa = 10.68 GKK139 pKa = 10.42 FGSGKK144 pKa = 9.21 FRR146 pKa = 11.84 KK147 pKa = 7.8 TPRR150 pKa = 11.84 RR151 pKa = 3.55
Molecular weight: 17.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.265
IPC2_protein 10.335
IPC_protein 11.096
Toseland 11.213
ProMoST 11.213
Dawson 11.272
Bjellqvist 11.038
Wikipedia 11.535
Rodwell 11.579
Grimsley 11.316
Solomon 11.477
Lehninger 11.433
Nozaki 11.184
DTASelect 11.038
Thurlkill 11.199
EMBOSS 11.637
Sillero 11.213
Patrickios 11.286
IPC_peptide 11.477
IPC2_peptide 9.984
IPC2.peptide.svr19 8.027
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
704
151
318
234.7
26.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.102 ± 1.39
1.42 ± 0.423
5.54 ± 0.774
4.403 ± 1.201
3.409 ± 0.196
8.523 ± 0.406
1.847 ± 0.616
5.682 ± 0.794
8.239 ± 1.616
6.25 ± 0.662
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.42 ± 0.111
4.119 ± 0.964
4.545 ± 1.724
2.841 ± 0.248
6.108 ± 1.537
8.949 ± 1.374
8.097 ± 1.183
4.688 ± 1.358
1.847 ± 0.158
4.972 ± 0.61
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here