Wenzhou picorna-like virus 28
Average proteome isoelectric point is 5.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KMH1|A0A1L3KMH1_9VIRU Uncharacterized protein OS=Wenzhou picorna-like virus 28 OX=1923613 PE=4 SV=1
MM1 pKa = 7.66 LSLKK5 pKa = 10.93 DD6 pKa = 3.5 MIKK9 pKa = 10.43 EE10 pKa = 3.87 KK11 pKa = 10.91 RR12 pKa = 11.84 PDD14 pKa = 3.72 DD15 pKa = 4.21 KK16 pKa = 10.44 MTLNRR21 pKa = 11.84 LRR23 pKa = 11.84 EE24 pKa = 3.98 FCKK27 pKa = 10.66 LFDD30 pKa = 4.4 DD31 pKa = 4.08 TTITHH36 pKa = 6.94 DD37 pKa = 3.47 VDD39 pKa = 3.99 DD40 pKa = 5.05 GRR42 pKa = 11.84 FSDD45 pKa = 4.53 FKK47 pKa = 11.11 VRR49 pKa = 11.84 CKK51 pKa = 10.32 GTDD54 pKa = 3.73 FILRR58 pKa = 11.84 LICDD62 pKa = 4.21 DD63 pKa = 4.25 GNIVDD68 pKa = 4.92 LGLMEE73 pKa = 6.36 FPDD76 pKa = 4.63 CDD78 pKa = 3.55 LFQPSFVSEE87 pKa = 4.05 VGEE90 pKa = 4.26 NTNKK94 pKa = 10.66 DD95 pKa = 3.17 QGGLVDD101 pKa = 4.77 ASTDD105 pKa = 3.22 RR106 pKa = 11.84 STMQDD111 pKa = 3.04 PTSNIVEE118 pKa = 4.47 ADD120 pKa = 3.79 PGPAAMPQEE129 pKa = 4.04 ILTFVDD135 pKa = 3.98 DD136 pKa = 3.92 TATQKK141 pKa = 11.28 ASLPEE146 pKa = 4.14 YY147 pKa = 9.36 TSPQASVLVPGSEE160 pKa = 4.08 LRR162 pKa = 11.84 NHH164 pKa = 6.73 TIEE167 pKa = 6.12 DD168 pKa = 3.2 ILKK171 pKa = 9.75 RR172 pKa = 11.84 PTKK175 pKa = 9.91 IQQLTWSTSQLKK187 pKa = 8.29 NTDD190 pKa = 3.02 IYY192 pKa = 11.14 TVAFPDD198 pKa = 4.7 AIIDD202 pKa = 3.65 ASDD205 pKa = 3.69 IIKK208 pKa = 10.77 DD209 pKa = 3.32 KK210 pKa = 10.96 LQNFTYY216 pKa = 10.27 LRR218 pKa = 11.84 ADD220 pKa = 2.72 ICVRR224 pKa = 11.84 VIINASTFQQGKK236 pKa = 8.87 LLAYY240 pKa = 7.3 FAPFSRR246 pKa = 11.84 VVGTRR251 pKa = 11.84 GTLNEE256 pKa = 4.35 HH257 pKa = 6.67 LPAKK261 pKa = 9.43 TAFPHH266 pKa = 5.41 VVLDD270 pKa = 3.86 AATGNSGDD278 pKa = 3.82 LRR280 pKa = 11.84 IPFVNPYY287 pKa = 6.91 THH289 pKa = 6.74 YY290 pKa = 11.51 NLTTQHH296 pKa = 6.59 GDD298 pKa = 2.96 MGNLQITVLNPLKK311 pKa = 10.0 TLTDD315 pKa = 3.54 CTVTVFAWFEE325 pKa = 4.33 NIDD328 pKa = 3.93 LGVPTARR335 pKa = 11.84 PNQALPAARR344 pKa = 11.84 SVHH347 pKa = 6.09 KK348 pKa = 10.73 NHH350 pKa = 6.51 ITTSDD355 pKa = 2.98 KK356 pKa = 11.71 DD357 pKa = 3.34 MLFRR361 pKa = 11.84 LIKK364 pKa = 10.4 KK365 pKa = 9.56 GVIRR369 pKa = 11.84 VKK371 pKa = 10.83 KK372 pKa = 10.26 NADD375 pKa = 3.12 TSGLLEE381 pKa = 4.3 FLTPYY386 pKa = 10.56 KK387 pKa = 10.27 SQCDD391 pKa = 3.78 DD392 pKa = 4.36 EE393 pKa = 4.45 ITSLFEE399 pKa = 4.16 RR400 pKa = 11.84 LEE402 pKa = 3.74 IMPKK406 pKa = 9.91 YY407 pKa = 9.97 KK408 pKa = 10.67 SQIAEE413 pKa = 4.32 DD414 pKa = 3.92 KK415 pKa = 10.5 EE416 pKa = 4.3 KK417 pKa = 10.35 SQKK420 pKa = 11.01 GIVTEE425 pKa = 4.13 TLEE428 pKa = 4.75 HH429 pKa = 6.41 IGGAASGMTNLPLIGDD445 pKa = 3.62 VFKK448 pKa = 10.73 PVEE451 pKa = 4.31 WIANSASKK459 pKa = 10.85 VSALFGFSKK468 pKa = 8.8 PTSVEE473 pKa = 4.31 TQCKK477 pKa = 7.87 FQNVPGFGFTHH488 pKa = 6.89 TDD490 pKa = 3.23 GLDD493 pKa = 3.34 QSVMLACKK501 pKa = 9.76 QDD503 pKa = 3.3 NQIQQRR509 pKa = 11.84 GDD511 pKa = 3.33 LFGSQVDD518 pKa = 3.91 EE519 pKa = 3.81 MDD521 pKa = 3.02 INYY524 pKa = 9.47 IAAHH528 pKa = 5.55 SCWFQTFKK536 pKa = 9.09 WTTADD541 pKa = 3.44 NPLNGVTLNEE551 pKa = 4.27 IAVHH555 pKa = 6.37 PGICPFDD562 pKa = 4.35 DD563 pKa = 4.53 EE564 pKa = 5.33 NFQPTLTAFVSAPFRR579 pKa = 11.84 YY580 pKa = 8.5 WRR582 pKa = 11.84 GGLTYY587 pKa = 10.14 KK588 pKa = 10.62 VQVAKK593 pKa = 10.18 TSFHH597 pKa = 6.62 SGRR600 pKa = 11.84 IRR602 pKa = 11.84 IAYY605 pKa = 8.62 VPSGVLNQKK614 pKa = 10.34 YY615 pKa = 10.11 NLDD618 pKa = 3.59 TCYY621 pKa = 10.99 SWVLDD626 pKa = 4.48 LRR628 pKa = 11.84 TSDD631 pKa = 3.54 QIEE634 pKa = 3.97 FTIPYY639 pKa = 9.75 ISNTQYY645 pKa = 11.43 KK646 pKa = 9.38 EE647 pKa = 3.5 VDD649 pKa = 3.7 LAPVASEE656 pKa = 3.98 PRR658 pKa = 11.84 TEE660 pKa = 4.12 STTGILVTEE669 pKa = 4.22 VLNALRR675 pKa = 11.84 APDD678 pKa = 3.81 TVDD681 pKa = 2.98 QAVEE685 pKa = 3.63 INMWISGASDD695 pKa = 3.65 YY696 pKa = 11.36 QLAIPDD702 pKa = 3.8 FDD704 pKa = 4.53 RR705 pKa = 11.84 YY706 pKa = 10.57 RR707 pKa = 11.84 IGNGKK712 pKa = 8.66 EE713 pKa = 3.55 AASTVKK719 pKa = 10.74 NSLIPKK725 pKa = 9.13 KK726 pKa = 9.87 STVVSTKK733 pKa = 7.37 EE734 pKa = 4.12 TEE736 pKa = 4.21 KK737 pKa = 10.7 EE738 pKa = 4.28 VPDD741 pKa = 4.3 PVPDD745 pKa = 3.44 QTTQASHH752 pKa = 5.63 TAEE755 pKa = 4.05 QARR758 pKa = 11.84 KK759 pKa = 9.19 AVANLRR765 pKa = 11.84 MEE767 pKa = 4.6 TEE769 pKa = 3.65 RR770 pKa = 11.84 LAAEE774 pKa = 3.83 EE775 pKa = 4.15 EE776 pKa = 4.17 RR777 pKa = 11.84 RR778 pKa = 11.84 KK779 pKa = 10.2 EE780 pKa = 3.82 YY781 pKa = 10.51 QKK783 pKa = 11.33 RR784 pKa = 11.84 LDD786 pKa = 3.21 EE787 pKa = 4.39 YY788 pKa = 10.38 YY789 pKa = 10.44 AYY791 pKa = 10.08 EE792 pKa = 4.07 RR793 pKa = 11.84 ASRR796 pKa = 11.84 QPRR799 pKa = 11.84 STPDD803 pKa = 2.8 EE804 pKa = 4.82 DD805 pKa = 4.07 YY806 pKa = 10.69 DD807 pKa = 4.07 YY808 pKa = 11.8 SSGYY812 pKa = 9.73 EE813 pKa = 4.03 SQVLGNFQDD822 pKa = 3.25 QGFNDD827 pKa = 4.35 FSDD830 pKa = 3.8 AAQMFGMKK838 pKa = 8.98 STDD841 pKa = 3.22 QLTPKK846 pKa = 9.52 TLTIGEE852 pKa = 4.32 DD853 pKa = 3.47 VQNIRR858 pKa = 11.84 SLIKK862 pKa = 10.23 RR863 pKa = 11.84 FGLRR867 pKa = 11.84 GEE869 pKa = 4.11 AALTHH874 pKa = 7.1 RR875 pKa = 11.84 YY876 pKa = 7.05 NTFYY880 pKa = 11.32 VNNGFFGSPEE890 pKa = 4.01 EE891 pKa = 4.1 LSVVALDD898 pKa = 3.74 YY899 pKa = 10.75 FSWIYY904 pKa = 10.4 RR905 pKa = 11.84 FYY907 pKa = 11.12 RR908 pKa = 11.84 GGQRR912 pKa = 11.84 YY913 pKa = 8.64 KK914 pKa = 10.83 FILDD918 pKa = 3.33 PRR920 pKa = 11.84 VCNDD924 pKa = 3.67 AIFYY928 pKa = 6.2 QTKK931 pKa = 9.31 GAEE934 pKa = 4.34 GKK936 pKa = 9.83 PEE938 pKa = 3.78 FQIQYY943 pKa = 8.37 MPKK946 pKa = 10.02 KK947 pKa = 8.98 EE948 pKa = 4.01 VPSINVSGAYY958 pKa = 9.85 NADD961 pKa = 3.93 PLPKK965 pKa = 9.72 VGEE968 pKa = 4.37 MNSSDD973 pKa = 3.71 TPVPEE978 pKa = 4.86 PYY980 pKa = 10.09 HH981 pKa = 6.65 RR982 pKa = 11.84 GTNFSHH988 pKa = 7.68 RR989 pKa = 11.84 PFTSLNPMIEE999 pKa = 4.17 VTLPFYY1005 pKa = 11.22 ANTPILPISSDD1016 pKa = 3.83 DD1017 pKa = 3.88 GLALADD1023 pKa = 3.24 IRR1025 pKa = 11.84 YY1026 pKa = 8.47 NAVVLEE1032 pKa = 3.99 YY1033 pKa = 10.39 RR1034 pKa = 11.84 GVRR1037 pKa = 11.84 IEE1039 pKa = 4.12 DD1040 pKa = 3.19 VHH1042 pKa = 7.7 YY1043 pKa = 11.09 DD1044 pKa = 3.21 GTGFVATPSTDD1055 pKa = 3.2 TRR1057 pKa = 11.84 CEE1059 pKa = 3.42 IAYY1062 pKa = 7.9 YY1063 pKa = 9.19 TAAADD1068 pKa = 3.73 DD1069 pKa = 5.51 FSFGWLIGPPYY1080 pKa = 10.5 LKK1082 pKa = 10.4 EE1083 pKa = 3.91 NPPRR1087 pKa = 11.84 PKK1089 pKa = 10.65
Molecular weight: 122.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.021
IPC2_protein 5.054
IPC_protein 5.041
Toseland 4.965
ProMoST 5.194
Dawson 5.041
Bjellqvist 5.169
Wikipedia 4.927
Rodwell 4.94
Grimsley 4.889
Solomon 5.041
Lehninger 5.003
Nozaki 5.169
DTASelect 5.359
Thurlkill 4.978
EMBOSS 4.978
Sillero 5.232
Patrickios 4.012
IPC_peptide 5.054
IPC2_peptide 5.219
IPC2.peptide.svr19 5.223
Protein with the highest isoelectric point:
>tr|A0A1L3KMH1|A0A1L3KMH1_9VIRU Uncharacterized protein OS=Wenzhou picorna-like virus 28 OX=1923613 PE=4 SV=1
MM1 pKa = 7.07 AQIVFCSQCPLSLDD15 pKa = 3.48 EE16 pKa = 5.72 LKK18 pKa = 11.05 NQLRR22 pKa = 11.84 CIEE25 pKa = 4.16 RR26 pKa = 11.84 ALEE29 pKa = 4.06 FNTKK33 pKa = 10.06 AKK35 pKa = 10.15 EE36 pKa = 3.94 QATPEE41 pKa = 4.09 QVVTLQVMIRR51 pKa = 11.84 WLQNSDD57 pKa = 3.15 ALFDD61 pKa = 4.11 VEE63 pKa = 5.46 CLHH66 pKa = 7.26 SDD68 pKa = 3.81 KK69 pKa = 10.63 THH71 pKa = 5.17 QCCFRR76 pKa = 11.84 LGTSTFYY83 pKa = 10.74 YY84 pKa = 10.52 ARR86 pKa = 11.84 PLSYY90 pKa = 10.92 TDD92 pKa = 3.55 VSFSNTLIQFTKK104 pKa = 10.8 AVIHH108 pKa = 6.85 PDD110 pKa = 3.51 PLLDD114 pKa = 3.92 ADD116 pKa = 4.35 IEE118 pKa = 4.44 HH119 pKa = 6.44 FHH121 pKa = 6.28 NRR123 pKa = 11.84 FYY125 pKa = 10.99 QISEE129 pKa = 4.06 RR130 pKa = 11.84 EE131 pKa = 3.93 NLVKK135 pKa = 10.85 NFTFEE140 pKa = 4.29 ALHH143 pKa = 6.0 KK144 pKa = 10.3 RR145 pKa = 11.84 SRR147 pKa = 11.84 LDD149 pKa = 3.31 AMLAKK154 pKa = 10.36 LDD156 pKa = 3.52 IQTRR160 pKa = 11.84 LPSFWTSQSADD171 pKa = 3.91 DD172 pKa = 4.3 ISPNAAACLEE182 pKa = 4.16 QYY184 pKa = 10.83 PDD186 pKa = 4.46 ALFLDD191 pKa = 4.67 LLTDD195 pKa = 3.77 RR196 pKa = 11.84 EE197 pKa = 4.18 NFEE200 pKa = 4.75 VISAAAIDD208 pKa = 3.85 RR209 pKa = 11.84 LRR211 pKa = 11.84 SRR213 pKa = 11.84 IAQSDD218 pKa = 3.79 DD219 pKa = 3.15 QLDD222 pKa = 3.66 RR223 pKa = 11.84 ARR225 pKa = 11.84 LLKK228 pKa = 10.54 LVSILVRR235 pKa = 11.84 LQRR238 pKa = 11.84 SQMYY242 pKa = 9.34 LINVRR247 pKa = 11.84 RR248 pKa = 11.84 IRR250 pKa = 11.84 GRR252 pKa = 11.84 HH253 pKa = 5.87 LIYY256 pKa = 10.51 SYY258 pKa = 11.4 DD259 pKa = 3.82 VAGHH263 pKa = 5.88 TIEE266 pKa = 4.85 FVEE269 pKa = 4.91 LRR271 pKa = 11.84 NHH273 pKa = 6.79 DD274 pKa = 4.17 FLVSIPITTSQLLIAEE290 pKa = 4.77 FDD292 pKa = 3.85 DD293 pKa = 4.06 EE294 pKa = 5.21 GYY296 pKa = 10.38 SSEE299 pKa = 3.91 MLNVNVHH306 pKa = 6.78 HH307 pKa = 6.66 KK308 pKa = 10.51 FDD310 pKa = 3.99 WNSASPFVDD319 pKa = 4.48 SILGDD324 pKa = 3.43 VKK326 pKa = 10.55 KK327 pKa = 8.93 TVKK330 pKa = 10.34 DD331 pKa = 3.47 ASVYY335 pKa = 10.99 NMLVGIASNIFLIWRR350 pKa = 11.84 HH351 pKa = 5.82 LSDD354 pKa = 6.0 PITVGLSLINILTYY368 pKa = 10.22 FKK370 pKa = 10.82 VGYY373 pKa = 10.24 DD374 pKa = 3.55 LALRR378 pKa = 11.84 AADD381 pKa = 3.82 SIKK384 pKa = 10.95 AHH386 pKa = 6.01 VFTILDD392 pKa = 3.48 YY393 pKa = 11.09 FKK395 pKa = 10.63 TFRR398 pKa = 11.84 SQAADD403 pKa = 3.66 GYY405 pKa = 8.48 TALKK409 pKa = 9.71 TLMLPIVATITTFLSVLVSNRR430 pKa = 11.84 LPDD433 pKa = 3.3 QKK435 pKa = 9.75 TIKK438 pKa = 10.57 DD439 pKa = 3.73 SLDD442 pKa = 3.04 RR443 pKa = 11.84 MASFGRR449 pKa = 11.84 AINGFEE455 pKa = 3.79 KK456 pKa = 10.11 TYY458 pKa = 11.17 AFLGEE463 pKa = 4.36 WIGKK467 pKa = 9.77 AFDD470 pKa = 2.84 ICYY473 pKa = 10.67 AKK475 pKa = 10.02 ICGMPRR481 pKa = 11.84 EE482 pKa = 4.37 ALDD485 pKa = 3.53 IEE487 pKa = 4.94 PYY489 pKa = 10.2 IADD492 pKa = 3.21 IHH494 pKa = 6.84 QYY496 pKa = 10.01 FADD499 pKa = 3.42 IQDD502 pKa = 3.44 VVKK505 pKa = 10.64 RR506 pKa = 11.84 SNYY509 pKa = 10.5 DD510 pKa = 3.74 DD511 pKa = 3.34 ITIDD515 pKa = 3.18 MDD517 pKa = 4.18 LGDD520 pKa = 5.14 RR521 pKa = 11.84 IEE523 pKa = 4.16 NLYY526 pKa = 10.95 KK527 pKa = 10.61 KK528 pKa = 9.72 GLKK531 pKa = 10.12 YY532 pKa = 10.51 SEE534 pKa = 4.03 QLSVLKK540 pKa = 10.61 LSSAQCQPFYY550 pKa = 10.94 VHH552 pKa = 6.78 FRR554 pKa = 11.84 EE555 pKa = 4.74 LSKK558 pKa = 11.11 LYY560 pKa = 10.9 GKK562 pKa = 8.61 LTTEE566 pKa = 4.5 GARR569 pKa = 11.84 YY570 pKa = 8.72 FSPRR574 pKa = 11.84 TEE576 pKa = 3.71 PAVIHH581 pKa = 5.7 LHH583 pKa = 6.01 GDD585 pKa = 3.36 SGIGKK590 pKa = 9.71 SGLVYY595 pKa = 10.91 LLAQDD600 pKa = 4.84 LLATEE605 pKa = 4.25 EE606 pKa = 4.75 LEE608 pKa = 4.8 DD609 pKa = 3.79 EE610 pKa = 4.63 VVKK613 pKa = 10.42 QIYY616 pKa = 8.47 MRR618 pKa = 11.84 QTEE621 pKa = 3.88 QEE623 pKa = 4.13 YY624 pKa = 8.83 WDD626 pKa = 4.84 GYY628 pKa = 8.86 QGQRR632 pKa = 11.84 ICIYY636 pKa = 10.89 DD637 pKa = 3.8 DD638 pKa = 3.83 FGQRR642 pKa = 11.84 TDD644 pKa = 3.95 SSAKK648 pKa = 9.21 PNEE651 pKa = 3.88 EE652 pKa = 3.4 FMEE655 pKa = 4.83 IIRR658 pKa = 11.84 TGNIIPMMCHH668 pKa = 5.34 MASIAEE674 pKa = 4.36 KK675 pKa = 10.5 ARR677 pKa = 11.84 TPFVSKK683 pKa = 10.88 GIILTSNAATFDD695 pKa = 4.03 IKK697 pKa = 11.6 SMTHH701 pKa = 4.4 PTAYY705 pKa = 9.36 QRR707 pKa = 11.84 RR708 pKa = 11.84 RR709 pKa = 11.84 QLVAEE714 pKa = 4.33 VVVNPLYY721 pKa = 10.79 AEE723 pKa = 3.96 KK724 pKa = 11.12 VMVNGQPTLRR734 pKa = 11.84 LDD736 pKa = 3.39 PKK738 pKa = 10.35 KK739 pKa = 10.39 IKK741 pKa = 10.37 EE742 pKa = 3.94 ADD744 pKa = 3.71 LPNLADD750 pKa = 3.29 EE751 pKa = 5.25 VYY753 pKa = 9.76 FFHH756 pKa = 6.94 VVSRR760 pKa = 11.84 DD761 pKa = 3.21 TGRR764 pKa = 11.84 GEE766 pKa = 4.64 LVLDD770 pKa = 3.48 EE771 pKa = 5.54 HH772 pKa = 7.3 GALQKK777 pKa = 11.1 KK778 pKa = 9.0 LIPYY782 pKa = 9.97 SEE784 pKa = 4.31 FKK786 pKa = 10.98 KK787 pKa = 10.09 MAIKK791 pKa = 10.15 AYY793 pKa = 8.4 EE794 pKa = 4.12 SQYY797 pKa = 11.23 EE798 pKa = 3.95 MSKK801 pKa = 10.89 ARR803 pKa = 11.84 LEE805 pKa = 4.28 CLAEE809 pKa = 3.78 RR810 pKa = 11.84 AKK812 pKa = 10.57 KK813 pKa = 10.05 IKK815 pKa = 10.46 AEE817 pKa = 4.0 RR818 pKa = 11.84 EE819 pKa = 4.18 GFRR822 pKa = 11.84 SQNDD826 pKa = 3.86 DD827 pKa = 3.97 DD828 pKa = 4.99 PLTLAEE834 pKa = 4.18 YY835 pKa = 10.53 AAVKK839 pKa = 10.5 YY840 pKa = 8.77 FLQMHH845 pKa = 6.73 DD846 pKa = 4.39 CEE848 pKa = 4.64 FTDD851 pKa = 3.65 VAHH854 pKa = 7.06 EE855 pKa = 4.13 IKK857 pKa = 10.7 LPKK860 pKa = 10.27 TFDD863 pKa = 3.23 KK864 pKa = 11.12 FEE866 pKa = 5.49 KK867 pKa = 10.42 EE868 pKa = 4.01 DD869 pKa = 3.74 FTGKK873 pKa = 9.72 SCAQIMDD880 pKa = 4.95 YY881 pKa = 10.71 ILQNYY886 pKa = 8.15 PDD888 pKa = 4.45 AATFTPKK895 pKa = 10.26 FSEE898 pKa = 4.24 KK899 pKa = 9.77 VRR901 pKa = 11.84 KK902 pKa = 8.91 IAHH905 pKa = 7.13 DD906 pKa = 3.68 YY907 pKa = 10.73 FILGKK912 pKa = 10.16 EE913 pKa = 3.89 FAFRR917 pKa = 11.84 SAIKK921 pKa = 9.9 TSTYY925 pKa = 10.32 LSKK928 pKa = 10.76 LSEE931 pKa = 4.3 RR932 pKa = 11.84 FAHH935 pKa = 5.83 TLRR938 pKa = 11.84 ATIDD942 pKa = 3.3 ADD944 pKa = 4.0 TPGVRR949 pKa = 11.84 IAKK952 pKa = 9.2 YY953 pKa = 10.07 VGGIVAGVSAMIGGFLLYY971 pKa = 10.33 QRR973 pKa = 11.84 LNPGKK978 pKa = 9.81 IEE980 pKa = 3.95 PAKK983 pKa = 10.0 TEE985 pKa = 4.88 AIATTCKK992 pKa = 9.99 LAKK995 pKa = 10.39 LVDD998 pKa = 3.66 NEE1000 pKa = 4.09 YY1001 pKa = 11.44 DD1002 pKa = 4.19 EE1003 pKa = 4.63 ITLDD1007 pKa = 3.89 LATQIVGSPCLSDD1020 pKa = 5.61 CKK1022 pKa = 10.83 FCDD1025 pKa = 4.67 DD1026 pKa = 3.45 FRR1028 pKa = 11.84 QYY1030 pKa = 10.99 LGSSITYY1037 pKa = 10.07 DD1038 pKa = 3.47 CLDD1041 pKa = 3.83 PQSIIEE1047 pKa = 4.71 AGHH1050 pKa = 5.68 SLYY1053 pKa = 10.96 EE1054 pKa = 4.17 KK1055 pKa = 9.56 ATSFTFRR1062 pKa = 11.84 DD1063 pKa = 3.54 AVEE1066 pKa = 4.72 DD1067 pKa = 3.56 LSEE1070 pKa = 4.11 AKK1072 pKa = 10.38 KK1073 pKa = 10.74 YY1074 pKa = 10.11 DD1075 pKa = 3.62 ANRR1078 pKa = 11.84 TSAKK1082 pKa = 9.68 KK1083 pKa = 10.26 VNRR1086 pKa = 11.84 TEE1088 pKa = 3.99 GKK1090 pKa = 10.23 KK1091 pKa = 10.37 YY1092 pKa = 10.25 DD1093 pKa = 3.99 ASRR1096 pKa = 11.84 TNQKK1100 pKa = 9.79 RR1101 pKa = 11.84 VARR1104 pKa = 11.84 TEE1106 pKa = 3.58 ARR1108 pKa = 11.84 KK1109 pKa = 9.94 YY1110 pKa = 9.94 DD1111 pKa = 3.72 ANRR1114 pKa = 11.84 TSQRR1118 pKa = 11.84 HH1119 pKa = 3.95 TVRR1122 pKa = 11.84 TEE1124 pKa = 3.39 LRR1126 pKa = 11.84 QTQGMEE1132 pKa = 3.91 SEE1134 pKa = 4.78 AVYY1137 pKa = 11.26 DD1138 pKa = 4.05 MNQHH1142 pKa = 6.7 EE1143 pKa = 4.94 IIQKK1147 pKa = 10.19 KK1148 pKa = 7.65 VMKK1151 pKa = 10.39 NMYY1154 pKa = 9.29 RR1155 pKa = 11.84 VYY1157 pKa = 10.47 VPSRR1161 pKa = 11.84 RR1162 pKa = 11.84 AGTEE1166 pKa = 2.96 WRR1168 pKa = 11.84 SIVNLFFVQGRR1179 pKa = 11.84 TAVLPTHH1186 pKa = 6.85 AIEE1189 pKa = 4.94 RR1190 pKa = 11.84 IKK1192 pKa = 10.6 MADD1195 pKa = 3.52 KK1196 pKa = 10.29 ILLRR1200 pKa = 11.84 NTYY1203 pKa = 8.88 QKK1205 pKa = 10.93 QGMSINTSEE1214 pKa = 4.52 IDD1216 pKa = 3.59 FAPIMASKK1224 pKa = 10.44 RR1225 pKa = 11.84 SASIFGQKK1233 pKa = 9.66 NLEE1236 pKa = 4.11 KK1237 pKa = 10.47 DD1238 pKa = 2.97 ASLAKK1243 pKa = 10.45 FPIQIPMHH1251 pKa = 5.84 MDD1253 pKa = 2.82 IVKK1256 pKa = 10.33 LFATSQEE1263 pKa = 3.82 LSTVKK1268 pKa = 10.02 SCRR1271 pKa = 11.84 ATLVSLKK1278 pKa = 10.34 DD1279 pKa = 3.42 VHH1281 pKa = 8.02 DD1282 pKa = 5.07 DD1283 pKa = 4.68 DD1284 pKa = 4.44 IHH1286 pKa = 7.55 FSAQVFSDD1294 pKa = 3.77 VVAKK1298 pKa = 9.46 DD1299 pKa = 3.34 TFSYY1303 pKa = 9.9 QHH1305 pKa = 6.37 SNIDD1309 pKa = 3.24 GSEE1312 pKa = 3.81 RR1313 pKa = 11.84 LYY1315 pKa = 11.24 ALRR1318 pKa = 11.84 DD1319 pKa = 3.36 CWEE1322 pKa = 4.29 YY1323 pKa = 11.73 VCDD1326 pKa = 5.51 SEE1328 pKa = 6.27 PGDD1331 pKa = 4.08 CGSLLVLSSPAVQRR1345 pKa = 11.84 KK1346 pKa = 8.51 IVGFHH1351 pKa = 5.34 VAGKK1355 pKa = 9.55 AGKK1358 pKa = 9.54 GASTSLTGEE1367 pKa = 4.06 DD1368 pKa = 3.58 LMRR1371 pKa = 11.84 ALRR1374 pKa = 11.84 PSEE1377 pKa = 5.07 HH1378 pKa = 6.96 DD1379 pKa = 3.62 PDD1381 pKa = 3.66 WRR1383 pKa = 11.84 AHH1385 pKa = 6.74 CIRR1388 pKa = 11.84 VAPEE1392 pKa = 3.61 VGDD1395 pKa = 3.88 DD1396 pKa = 4.49 KK1397 pKa = 11.23 EE1398 pKa = 4.46 VKK1400 pKa = 10.24 LPQGDD1405 pKa = 5.08 FIPIGKK1411 pKa = 9.42 LPRR1414 pKa = 11.84 SYY1416 pKa = 10.97 RR1417 pKa = 11.84 GGNKK1421 pKa = 8.61 TKK1423 pKa = 10.53 LRR1425 pKa = 11.84 EE1426 pKa = 4.16 SPLQGALAYY1435 pKa = 10.69 NDD1437 pKa = 4.22 GKK1439 pKa = 9.11 TLTAPATLNRR1449 pKa = 11.84 IQVGDD1454 pKa = 3.94 EE1455 pKa = 4.01 LVDD1458 pKa = 3.83 PLEE1461 pKa = 4.71 KK1462 pKa = 10.47 GLRR1465 pKa = 11.84 KK1466 pKa = 10.13 CGVQPMPIPVEE1477 pKa = 4.01 LVNAACEE1484 pKa = 4.18 HH1485 pKa = 6.32 FSSKK1489 pKa = 10.35 LYY1491 pKa = 10.94 QNIDD1495 pKa = 3.22 PDD1497 pKa = 3.43 HH1498 pKa = 6.72 RR1499 pKa = 11.84 RR1500 pKa = 11.84 VLTHH1504 pKa = 6.55 EE1505 pKa = 4.36 EE1506 pKa = 4.37 SISGIPNDD1514 pKa = 4.95 SYY1516 pKa = 11.93 AEE1518 pKa = 4.55 PINRR1522 pKa = 11.84 RR1523 pKa = 11.84 SSPGYY1528 pKa = 9.3 PWIDD1532 pKa = 3.49 SAKK1535 pKa = 8.86 GTLGKK1540 pKa = 8.74 TKK1542 pKa = 9.83 WLGDD1546 pKa = 3.77 GEE1548 pKa = 4.63 EE1549 pKa = 4.48 YY1550 pKa = 10.16 IYY1552 pKa = 10.63 DD1553 pKa = 3.87 HH1554 pKa = 7.45 PEE1556 pKa = 3.69 LLTALKK1562 pKa = 9.68 TRR1564 pKa = 11.84 EE1565 pKa = 3.96 EE1566 pKa = 4.03 NAKK1569 pKa = 10.32 QGIRR1573 pKa = 11.84 TPTYY1577 pKa = 10.05 WIDD1580 pKa = 3.35 TLKK1583 pKa = 10.97 DD1584 pKa = 3.12 EE1585 pKa = 4.81 RR1586 pKa = 11.84 RR1587 pKa = 11.84 PIEE1590 pKa = 3.87 KK1591 pKa = 10.27 VQIGKK1596 pKa = 8.2 TRR1598 pKa = 11.84 VFAAGAMDD1606 pKa = 4.89 FIILFRR1612 pKa = 11.84 KK1613 pKa = 9.74 YY1614 pKa = 9.94 FLGFNAHH1621 pKa = 5.36 VMKK1624 pKa = 10.75 EE1625 pKa = 4.07 KK1626 pKa = 10.01 IDD1628 pKa = 3.6 NEE1630 pKa = 3.9 IAVGINVYY1638 pKa = 10.51 SPEE1641 pKa = 3.98 WNKK1644 pKa = 10.55 LGRR1647 pKa = 11.84 YY1648 pKa = 8.59 LKK1650 pKa = 10.38 RR1651 pKa = 11.84 QGAKK1655 pKa = 10.2 VIAGDD1660 pKa = 3.93 FSNFDD1665 pKa = 3.39 GTLNAQILHH1674 pKa = 6.88 KK1675 pKa = 10.36 ICDD1678 pKa = 4.76 IINEE1682 pKa = 4.28 WYY1684 pKa = 10.09 DD1685 pKa = 3.89 DD1686 pKa = 3.97 GPEE1689 pKa = 3.7 NARR1692 pKa = 11.84 IRR1694 pKa = 11.84 KK1695 pKa = 8.39 VMWEE1699 pKa = 4.26 EE1700 pKa = 4.06 IVSSHH1705 pKa = 7.25 HH1706 pKa = 6.02 IFEE1709 pKa = 5.4 DD1710 pKa = 3.84 NVYY1713 pKa = 10.02 SWTHH1717 pKa = 5.22 SQPSGNPCTVIINSMYY1733 pKa = 10.61 NSISMRR1739 pKa = 11.84 IVWQLLMAGTQHH1751 pKa = 7.05 AALSNFSKK1759 pKa = 9.8 YY1760 pKa = 9.8 VNMISFGDD1768 pKa = 3.88 DD1769 pKa = 2.73 NVLNINDD1776 pKa = 3.88 LVIDD1780 pKa = 4.73 RR1781 pKa = 11.84 FNQLTIAEE1789 pKa = 5.13 GYY1791 pKa = 9.01 AQIGMTYY1798 pKa = 9.11 TDD1800 pKa = 3.41 EE1801 pKa = 4.69 GKK1803 pKa = 8.65 TGEE1806 pKa = 4.22 MVKK1809 pKa = 10.4 YY1810 pKa = 10.18 RR1811 pKa = 11.84 ALEE1814 pKa = 3.81 DD1815 pKa = 3.44 VKK1817 pKa = 10.73 FLKK1820 pKa = 10.55 RR1821 pKa = 11.84 GFRR1824 pKa = 11.84 YY1825 pKa = 9.82 EE1826 pKa = 3.9 KK1827 pKa = 10.53 GVYY1830 pKa = 9.11 LAPLEE1835 pKa = 4.45 LDD1837 pKa = 3.66 VVMEE1841 pKa = 3.96 MCCWVKK1847 pKa = 10.39 TDD1849 pKa = 4.59 VNTIDD1854 pKa = 3.42 NTITNVEE1861 pKa = 3.92 TAMRR1865 pKa = 11.84 EE1866 pKa = 4.12 LSLHH1870 pKa = 6.21 PKK1872 pKa = 10.46 DD1873 pKa = 5.25 VFTKK1877 pKa = 10.59 CKK1879 pKa = 10.44 ADD1881 pKa = 3.74 LLQACRR1887 pKa = 11.84 KK1888 pKa = 8.88 HH1889 pKa = 6.73 LPRR1892 pKa = 11.84 QPEE1895 pKa = 3.98 TLTYY1899 pKa = 10.3 EE1900 pKa = 4.14 DD1901 pKa = 4.88 HH1902 pKa = 7.18 RR1903 pKa = 11.84 LQDD1906 pKa = 3.48 FEE1908 pKa = 6.38 SYY1910 pKa = 9.67 FF1911 pKa = 3.56
Molecular weight: 217.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.021
IPC2_protein 6.008
IPC_protein 6.084
Toseland 6.402
ProMoST 6.465
Dawson 6.389
Bjellqvist 6.402
Wikipedia 6.415
Rodwell 6.389
Grimsley 6.547
Solomon 6.402
Lehninger 6.389
Nozaki 6.678
DTASelect 6.854
Thurlkill 6.868
EMBOSS 6.839
Sillero 6.781
Patrickios 4.202
IPC_peptide 6.415
IPC2_peptide 6.664
IPC2.peptide.svr19 6.691
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3000
1089
1911
1500.0
169.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.4 ± 0.297
1.567 ± 0.163
7.3 ± 0.186
5.967 ± 0.264
4.7 ± 0.256
5.167 ± 0.305
2.233 ± 0.336
6.467 ± 0.447
6.233 ± 0.472
8.4 ± 0.503
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.133 ± 0.225
4.067 ± 0.356
4.567 ± 0.864
4.2 ± 0.173
5.5 ± 0.366
6.5 ± 0.042
6.633 ± 0.89
6.0 ± 0.194
0.933 ± 0.009
4.033 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here