Bacillus phage Tavor_SA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wbetavirus; unclassified Wbetavirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A288WFV0|A0A288WFV0_9CAUD Uncharacterized protein OS=Bacillus phage Tavor_SA OX=1983581 PE=4 SV=1
MM1 pKa = 7.39WMVHH5 pKa = 6.59DD6 pKa = 4.24YY7 pKa = 11.51EE8 pKa = 4.66EE9 pKa = 4.9GVVLITDD16 pKa = 3.76NYY18 pKa = 9.91EE19 pKa = 3.94EE20 pKa = 4.24ALKK23 pKa = 10.45EE24 pKa = 3.85YY25 pKa = 10.25EE26 pKa = 4.73KK27 pKa = 11.02YY28 pKa = 10.57VEE30 pKa = 4.3SAKK33 pKa = 11.01DD34 pKa = 3.22WVQEE38 pKa = 4.1NGCEE42 pKa = 3.89FDD44 pKa = 3.47GEE46 pKa = 4.29EE47 pKa = 3.88RR48 pKa = 11.84VILAKK53 pKa = 10.38LEE55 pKa = 3.95RR56 pKa = 11.84QAYY59 pKa = 9.36GSATGRR65 pKa = 11.84TISGSTYY72 pKa = 10.8DD73 pKa = 3.16EE74 pKa = 4.33WDD76 pKa = 3.29WKK78 pKa = 9.28EE79 pKa = 3.68EE80 pKa = 4.03KK81 pKa = 10.62YY82 pKa = 11.1

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A288WG52|A0A288WG52_9CAUD Site-specific recombinase OS=Bacillus phage Tavor_SA OX=1983581 PE=4 SV=1
MM1 pKa = 7.26RR2 pKa = 11.84TWKK5 pKa = 10.13KK6 pKa = 10.35KK7 pKa = 7.96HH8 pKa = 5.1VKK10 pKa = 9.81RR11 pKa = 11.84AFLNRR16 pKa = 11.84QKK18 pKa = 10.85EE19 pKa = 3.9IDD21 pKa = 3.67KK22 pKa = 10.74EE23 pKa = 4.02RR24 pKa = 11.84TAAAWRR30 pKa = 11.84NIFVKK35 pKa = 10.61SGIIKK40 pKa = 10.38

Molecular weight: 4.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

12186

36

1332

199.8

22.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.122 ± 0.483

0.952 ± 0.164

5.547 ± 0.217

9.084 ± 0.38

4.07 ± 0.195

5.769 ± 0.276

1.633 ± 0.173

7.139 ± 0.325

9.536 ± 0.341

8.346 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.158

5.334 ± 0.224

2.552 ± 0.233

4.103 ± 0.281

4.94 ± 0.253

5.72 ± 0.219

5.293 ± 0.337

6.245 ± 0.274

1.124 ± 0.151

3.758 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski