Abditibacterium utsteinense
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3182 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S8SRM1|A0A2S8SRM1_9BACT Phosphate transport system permease protein OS=Abditibacterium utsteinense OX=1960156 GN=B1R32_11292 PE=3 SV=1
MM1 pKa = 7.82 INITDD6 pKa = 3.54 TAAAKK11 pKa = 10.33 VHH13 pKa = 6.25 GLLQAQEE20 pKa = 4.7 KK21 pKa = 8.38 PNAMLRR27 pKa = 11.84 VFVSGGGCSGYY38 pKa = 10.57 SYY40 pKa = 11.61 GMTLEE45 pKa = 4.15 EE46 pKa = 4.92 DD47 pKa = 3.62 AMEE50 pKa = 5.07 GDD52 pKa = 3.62 QQFSVNGVGVIIDD65 pKa = 3.88 PRR67 pKa = 11.84 SAQYY71 pKa = 11.19 LEE73 pKa = 4.22 GANIDD78 pKa = 3.97 FVDD81 pKa = 3.31 NMMGGGFKK89 pKa = 9.71 IDD91 pKa = 3.75 NPNAASSCGCGSSFTPKK108 pKa = 9.92 EE109 pKa = 3.98 GQAEE113 pKa = 4.39 GEE115 pKa = 4.07 EE116 pKa = 4.38 AMAGAAAGGGCGSCSSGGGFF136 pKa = 4.34
Molecular weight: 13.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.899
IPC2_protein 4.177
IPC_protein 4.062
Toseland 3.884
ProMoST 4.19
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.923
Rodwell 3.897
Grimsley 3.795
Solomon 4.012
Lehninger 3.973
Nozaki 4.151
DTASelect 4.291
Thurlkill 3.923
EMBOSS 3.935
Sillero 4.177
Patrickios 0.896
IPC_peptide 4.012
IPC2_peptide 4.164
IPC2.peptide.svr19 4.069
Protein with the highest isoelectric point:
>tr|A0A2S8SSX1|A0A2S8SSX1_9BACT Endonuclease Uma2 family (Restriction endonuclease fold) OS=Abditibacterium utsteinense OX=1960156 GN=B1R32_10883 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.47 RR3 pKa = 11.84 TWQPKK8 pKa = 8.03 KK9 pKa = 10.1 RR10 pKa = 11.84 PRR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.61 LGFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSSHH25 pKa = 6.67 GGRR28 pKa = 11.84 AIISRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.67 GRR39 pKa = 11.84 IKK41 pKa = 9.98 LTQVV45 pKa = 2.62
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3182
0
3182
1065418
25
3129
334.8
36.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.094 ± 0.052
0.936 ± 0.015
4.954 ± 0.029
6.031 ± 0.047
4.325 ± 0.029
7.909 ± 0.035
1.874 ± 0.022
5.233 ± 0.031
4.309 ± 0.034
10.234 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.038 ± 0.022
3.626 ± 0.026
5.136 ± 0.035
3.931 ± 0.025
6.411 ± 0.04
6.472 ± 0.035
5.211 ± 0.038
6.635 ± 0.037
1.458 ± 0.023
2.183 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here