Glis glis polyomavirus 1
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1CJL7|A0A2S1CJL7_9POLY Large T antigen OS=Glis glis polyomavirus 1 OX=2170404 GN=650T PE=4 SV=1
MM1 pKa = 7.62 LRR3 pKa = 11.84 ILFQKK8 pKa = 9.91 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 GEE13 pKa = 3.86 EE14 pKa = 3.5 EE15 pKa = 4.2 GPRR18 pKa = 11.84 EE19 pKa = 4.02 GGAAVAAAAAGNGGAPSDD37 pKa = 4.19 TEE39 pKa = 3.93 PTTPARR45 pKa = 11.84 NIASIRR51 pKa = 11.84 LQEE54 pKa = 4.18 VAPGPEE60 pKa = 4.38 DD61 pKa = 3.51 KK62 pKa = 10.63 PGPEE66 pKa = 4.21 DD67 pKa = 3.62 TSDD70 pKa = 3.6 TEE72 pKa = 4.33 DD73 pKa = 3.34 TLGTEE78 pKa = 4.45 DD79 pKa = 4.18 TSDD82 pKa = 3.81 TEE84 pKa = 4.41 DD85 pKa = 3.59 TPSPEE90 pKa = 3.45 VWGEE94 pKa = 3.59 YY95 pKa = 9.71 LQYY98 pKa = 11.57
Molecular weight: 10.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.433
IPC2_protein 4.177
IPC_protein 4.075
Toseland 3.91
ProMoST 4.202
Dawson 4.024
Bjellqvist 4.19
Wikipedia 3.91
Rodwell 3.91
Grimsley 3.821
Solomon 4.024
Lehninger 3.973
Nozaki 4.151
DTASelect 4.279
Thurlkill 3.923
EMBOSS 3.923
Sillero 4.19
Patrickios 3.961
IPC_peptide 4.024
IPC2_peptide 4.177
IPC2.peptide.svr19 4.114
Protein with the highest isoelectric point:
>tr|A0A2S1CJL3|A0A2S1CJL3_9POLY Minor capsid protein VP2 OS=Glis glis polyomavirus 1 OX=2170404 GN=VP2 PE=3 SV=1
MM1 pKa = 7.61 ALAPYY6 pKa = 10.24 NPDD9 pKa = 2.62 IDD11 pKa = 4.35 YY12 pKa = 10.81 DD13 pKa = 3.67 ILFPGVSWFARR24 pKa = 11.84 NIRR27 pKa = 11.84 YY28 pKa = 8.54 FDD30 pKa = 3.31 PTFWTTRR37 pKa = 11.84 LWQRR41 pKa = 11.84 FVEE44 pKa = 4.53 GLQRR48 pKa = 11.84 EE49 pKa = 4.49 AVRR52 pKa = 11.84 QIAHH56 pKa = 5.66 EE57 pKa = 4.22 TNALGARR64 pKa = 11.84 ARR66 pKa = 11.84 EE67 pKa = 4.06 QAALTLRR74 pKa = 11.84 EE75 pKa = 3.68 AVARR79 pKa = 11.84 VVEE82 pKa = 4.11 NTQWVFTNLGQGASNLYY99 pKa = 10.6 ASVEE103 pKa = 4.06 RR104 pKa = 11.84 YY105 pKa = 9.14 YY106 pKa = 11.56 AEE108 pKa = 4.86 LPGLNPAQRR117 pKa = 11.84 RR118 pKa = 11.84 ALMEE122 pKa = 4.15 RR123 pKa = 11.84 LPRR126 pKa = 11.84 EE127 pKa = 4.58 DD128 pKa = 3.53 IVVPQQDD135 pKa = 4.29 SLPQSGDD142 pKa = 3.16 FVVRR146 pKa = 11.84 YY147 pKa = 7.0 EE148 pKa = 4.34 APGGANQRR156 pKa = 11.84 SCPNWLLPLILGIYY170 pKa = 7.28 DD171 pKa = 3.98 TTWGEE176 pKa = 4.21 PVSKK180 pKa = 10.3 HH181 pKa = 3.42 ATKK184 pKa = 10.41 RR185 pKa = 11.84 KK186 pKa = 9.93 SSAEE190 pKa = 3.72 DD191 pKa = 3.09 TGPKK195 pKa = 8.9 TNCKK199 pKa = 9.65 RR200 pKa = 11.84 KK201 pKa = 9.8 RR202 pKa = 11.84 GSPGSKK208 pKa = 9.83 NRR210 pKa = 11.84 AA211 pKa = 3.29
Molecular weight: 23.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.886
IPC2_protein 8.902
IPC_protein 9.033
Toseland 9.311
ProMoST 9.253
Dawson 9.663
Bjellqvist 9.472
Wikipedia 9.897
Rodwell 9.75
Grimsley 9.765
Solomon 9.736
Lehninger 9.692
Nozaki 9.443
DTASelect 9.428
Thurlkill 9.487
EMBOSS 9.765
Sillero 9.619
Patrickios 4.876
IPC_peptide 9.721
IPC2_peptide 8.331
IPC2.peptide.svr19 7.797
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1801
98
650
300.2
33.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.385 ± 1.295
2.221 ± 0.554
5.33 ± 0.542
6.885 ± 0.584
3.776 ± 0.527
6.774 ± 0.908
1.943 ± 0.593
4.553 ± 0.369
4.942 ± 0.774
9.606 ± 0.644
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.554 ± 0.53
4.997 ± 0.343
5.941 ± 0.794
4.442 ± 0.608
6.163 ± 0.54
5.497 ± 0.176
6.496 ± 0.355
5.386 ± 0.654
2.054 ± 0.257
3.054 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here