Magnetospirillum aberrantis SpK
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3840 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7C9UVQ7|A0A7C9UVQ7_9PROT FeoC domain-containing protein OS=Magnetospirillum aberrantis SpK OX=908842 GN=G4223_05660 PE=4 SV=1
MM1 pKa = 7.07 TLSSAMTTAVSALKK15 pKa = 10.36 AQSQALSAISNNLANSSTTGYY36 pKa = 10.93 KK37 pKa = 10.14 SVTTSFQSLVTQAFSGTAYY56 pKa = 9.32 TGAGVTSSVRR66 pKa = 11.84 QNVDD70 pKa = 2.57 AQGNIEE76 pKa = 4.09 ATSNVTDD83 pKa = 3.76 MAISGDD89 pKa = 3.46 GMFVVTDD96 pKa = 3.45 SSGAQYY102 pKa = 8.15 YY103 pKa = 8.18 TRR105 pKa = 11.84 NGEE108 pKa = 3.9 FDD110 pKa = 3.56 VDD112 pKa = 4.06 DD113 pKa = 6.39 DD114 pKa = 4.94 GYY116 pKa = 11.42 LYY118 pKa = 11.32 NNASTKK124 pKa = 10.35 VYY126 pKa = 10.85 LMGYY130 pKa = 9.65 AINSDD135 pKa = 2.99 GTTAKK140 pKa = 9.63 TLSQINVTDD149 pKa = 4.04 QDD151 pKa = 4.27 TSWKK155 pKa = 7.72 EE156 pKa = 4.0 TTAVSLDD163 pKa = 3.37 ASLGADD169 pKa = 3.34 TDD171 pKa = 4.18 VSSSTTYY178 pKa = 10.23 EE179 pKa = 3.96 KK180 pKa = 10.4 TFEE183 pKa = 4.7 VYY185 pKa = 10.82 DD186 pKa = 3.84 SMGGSHH192 pKa = 7.09 IVTATFAHH200 pKa = 5.84 TAVDD204 pKa = 3.94 SSANTSTWTVSFSSDD219 pKa = 4.44 DD220 pKa = 3.49 GTTTLDD226 pKa = 3.32 TLTLEE231 pKa = 4.43 FDD233 pKa = 3.77 GDD235 pKa = 4.23 GNLTSATDD243 pKa = 3.61 SSNVTVDD250 pKa = 3.79 PSSLTLDD257 pKa = 4.0 FDD259 pKa = 4.34 WNNASSDD266 pKa = 3.57 SSITLDD272 pKa = 3.75 LSSISMGGSTDD283 pKa = 3.64 GVTLNDD289 pKa = 3.64 VTNDD293 pKa = 3.35 GYY295 pKa = 11.52 EE296 pKa = 3.94 LGNLSGITISSDD308 pKa = 3.23 GTVSASYY315 pKa = 11.43 DD316 pKa = 3.37 NGQTISLYY324 pKa = 9.98 KK325 pKa = 10.27 VAVATFANYY334 pKa = 10.21 DD335 pKa = 3.47 GLAALSGTLYY345 pKa = 10.54 QQTADD350 pKa = 3.56 SGEE353 pKa = 4.39 AIIGTAEE360 pKa = 3.95 TGSAGSISASSLEE373 pKa = 4.16 SSTVDD378 pKa = 2.94 TADD381 pKa = 2.92 EE382 pKa = 4.26 FTRR385 pKa = 11.84 MIVAQQAYY393 pKa = 9.14 SAASQVITTAKK404 pKa = 10.62 DD405 pKa = 3.01 MYY407 pKa = 10.46 DD408 pKa = 3.38 ALISAVRR415 pKa = 3.45
Molecular weight: 42.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.528
IPC_protein 3.579
Toseland 3.338
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.745
Wikipedia 3.567
Rodwell 3.401
Grimsley 3.236
Solomon 3.579
Lehninger 3.541
Nozaki 3.706
DTASelect 4.012
Thurlkill 3.401
EMBOSS 3.567
Sillero 3.706
Patrickios 0.579
IPC_peptide 3.567
IPC2_peptide 3.668
IPC2.peptide.svr19 3.661
Protein with the highest isoelectric point:
>tr|A0A7C9QRW5|A0A7C9QRW5_9PROT DUF2927 domain-containing protein OS=Magnetospirillum aberrantis SpK OX=908842 GN=G4223_01420 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.43 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.38 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.33 VIATRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3840
0
3840
1252964
29
6672
326.3
35.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.765 ± 0.058
0.943 ± 0.016
5.965 ± 0.039
5.714 ± 0.042
3.404 ± 0.026
8.552 ± 0.056
2.137 ± 0.024
4.294 ± 0.029
3.205 ± 0.031
10.49 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.545 ± 0.02
2.476 ± 0.028
5.094 ± 0.046
3.176 ± 0.024
7.215 ± 0.06
5.144 ± 0.042
5.32 ± 0.061
8.189 ± 0.037
1.353 ± 0.016
2.018 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here