Bacteroidetes oral taxon 274 str. F0058
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1854 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D7JE57|D7JE57_9BACT Stage 0 sporulation protein YaaT OS=Bacteroidetes oral taxon 274 str. F0058 OX=575590 GN=HMPREF0156_00765 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.11 LASKK10 pKa = 11.0 LEE12 pKa = 4.24 DD13 pKa = 4.06 APWPATKK20 pKa = 10.54 DD21 pKa = 3.54 EE22 pKa = 5.18 LIDD25 pKa = 3.42 YY26 pKa = 10.58 AMRR29 pKa = 11.84 TGAPLEE35 pKa = 4.37 VIEE38 pKa = 4.31 NLQGIEE44 pKa = 4.51 DD45 pKa = 3.99 EE46 pKa = 4.7 GDD48 pKa = 3.43 VYY50 pKa = 11.85 DD51 pKa = 5.05 NIEE54 pKa = 5.34 DD55 pKa = 3.31 IWPDD59 pKa = 3.62 YY60 pKa = 10.2 PSKK63 pKa = 11.18 EE64 pKa = 3.66 DD65 pKa = 3.46 FFFNEE70 pKa = 4.55 DD71 pKa = 3.13 EE72 pKa = 4.46 YY73 pKa = 11.99
Molecular weight: 8.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.857
IPC2_protein 3.567
IPC_protein 3.503
Toseland 3.312
ProMoST 3.681
Dawson 3.503
Bjellqvist 3.656
Wikipedia 3.439
Rodwell 3.338
Grimsley 3.223
Solomon 3.465
Lehninger 3.414
Nozaki 3.63
DTASelect 3.795
Thurlkill 3.376
EMBOSS 3.439
Sillero 3.63
Patrickios 1.774
IPC_peptide 3.465
IPC2_peptide 3.592
IPC2.peptide.svr19 3.654
Protein with the highest isoelectric point:
>tr|D7JFV9|D7JFV9_9BACT Sporulation protein SpoIID-related protein OS=Bacteroidetes oral taxon 274 str. F0058 OX=575590 GN=HMPREF0156_01789 PE=4 SV=1
MM1 pKa = 7.5 AKK3 pKa = 10.25 EE4 pKa = 3.96 SMKK7 pKa = 10.66 ARR9 pKa = 11.84 EE10 pKa = 4.12 VKK12 pKa = 9.96 RR13 pKa = 11.84 AKK15 pKa = 10.25 LVARR19 pKa = 11.84 YY20 pKa = 9.45 AEE22 pKa = 4.41 KK23 pKa = 10.04 RR24 pKa = 11.84 KK25 pKa = 10.3 QMIAEE30 pKa = 4.38 GDD32 pKa = 3.49 YY33 pKa = 11.41 DD34 pKa = 4.36 ALQTLPRR41 pKa = 11.84 NSSYY45 pKa = 10.67 IRR47 pKa = 11.84 LHH49 pKa = 5.99 NRR51 pKa = 11.84 CKK53 pKa = 8.78 MTGRR57 pKa = 11.84 PKK59 pKa = 10.81 GYY61 pKa = 8.37 MRR63 pKa = 11.84 QFGISRR69 pKa = 11.84 IQFRR73 pKa = 11.84 EE74 pKa = 3.94 MASKK78 pKa = 11.0 GLIPGVKK85 pKa = 9.19 KK86 pKa = 10.96 ASWW89 pKa = 3.03
Molecular weight: 10.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.94
IPC_protein 10.672
Toseland 10.935
ProMoST 10.599
Dawson 11.008
Bjellqvist 10.701
Wikipedia 11.213
Rodwell 11.359
Grimsley 11.052
Solomon 11.125
Lehninger 11.096
Nozaki 10.906
DTASelect 10.701
Thurlkill 10.921
EMBOSS 11.33
Sillero 10.935
Patrickios 11.082
IPC_peptide 11.14
IPC2_peptide 9.531
IPC2.peptide.svr19 8.453
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1854
0
1854
603910
38
2484
325.7
36.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.917 ± 0.052
1.183 ± 0.025
5.824 ± 0.038
6.079 ± 0.059
4.769 ± 0.045
6.427 ± 0.049
1.692 ± 0.024
8.001 ± 0.061
7.196 ± 0.054
8.727 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.465 ± 0.023
5.513 ± 0.051
3.354 ± 0.027
3.199 ± 0.033
4.68 ± 0.043
6.335 ± 0.047
5.657 ± 0.045
6.648 ± 0.043
0.967 ± 0.019
4.364 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here