Human immunodeficiency virus type 1 group M subtype H (isolate 90CF056) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 M:H

Average proteome isoelectric point is 7.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O70902|ENV_HV190 Envelope glycoprotein gp160 OS=Human immunodeficiency virus type 1 group M subtype H (isolate 90CF056) OX=388826 GN=env PE=3 SV=1
MM1 pKa = 7.52YY2 pKa = 10.38ILGLGIGALVVTFIIAVIVWTIVYY26 pKa = 9.69IEE28 pKa = 4.09YY29 pKa = 10.6KK30 pKa = 10.45KK31 pKa = 10.73LVRR34 pKa = 11.84QKK36 pKa = 10.87KK37 pKa = 8.47IDD39 pKa = 3.65RR40 pKa = 11.84LIEE43 pKa = 4.57RR44 pKa = 11.84IGEE47 pKa = 4.06RR48 pKa = 11.84AEE50 pKa = 4.51DD51 pKa = 3.7SGNEE55 pKa = 3.97SDD57 pKa = 5.62GDD59 pKa = 3.87TEE61 pKa = 4.18EE62 pKa = 4.43LSKK65 pKa = 11.04LMEE68 pKa = 4.5MGHH71 pKa = 6.81LNLGYY76 pKa = 10.6VADD79 pKa = 4.39LL80 pKa = 4.04

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O70898|VPR_HV190 Protein Vpr OS=Human immunodeficiency virus type 1 group M subtype H (isolate 90CF056) OX=388826 GN=vpr PE=3 SV=1
MM1 pKa = 7.3EE2 pKa = 5.21NRR4 pKa = 11.84WQVMIVWQVDD14 pKa = 3.06RR15 pKa = 11.84MRR17 pKa = 11.84INTWKK22 pKa = 10.86SLVKK26 pKa = 9.87YY27 pKa = 10.05HH28 pKa = 5.37MHH30 pKa = 6.89ISRR33 pKa = 11.84KK34 pKa = 8.91ARR36 pKa = 11.84GWFYY40 pKa = 10.74RR41 pKa = 11.84HH42 pKa = 6.17HH43 pKa = 7.06FEE45 pKa = 4.19STHH48 pKa = 5.59PRR50 pKa = 11.84ISSEE54 pKa = 3.7VHH56 pKa = 5.5IPLGEE61 pKa = 4.02ARR63 pKa = 11.84LVITTYY69 pKa = 9.8WGLNTGEE76 pKa = 4.63RR77 pKa = 11.84EE78 pKa = 3.75WHH80 pKa = 6.3LGQGVSIEE88 pKa = 4.02WRR90 pKa = 11.84LKK92 pKa = 9.71RR93 pKa = 11.84YY94 pKa = 7.21STQVEE99 pKa = 4.3PGLADD104 pKa = 3.48QLIHH108 pKa = 5.73MHH110 pKa = 6.2YY111 pKa = 9.75FDD113 pKa = 5.42CFSEE117 pKa = 4.35SAIRR121 pKa = 11.84KK122 pKa = 8.74AILGRR127 pKa = 11.84VVRR130 pKa = 11.84PRR132 pKa = 11.84CNYY135 pKa = 8.68PAGHH139 pKa = 6.15KK140 pKa = 9.88QVGTLQYY147 pKa = 10.88LALTALVAPKK157 pKa = 9.95KK158 pKa = 10.01IKK160 pKa = 10.26PPLPSVRR167 pKa = 11.84KK168 pKa = 9.46LVEE171 pKa = 4.17DD172 pKa = 4.23RR173 pKa = 11.84WNKK176 pKa = 7.26PQKK179 pKa = 9.1TRR181 pKa = 11.84GHH183 pKa = 6.43RR184 pKa = 11.84GSHH187 pKa = 4.85TMNGHH192 pKa = 6.05

Molecular weight: 22.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3641

72

1435

364.1

41.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.262 ± 0.674

2.06 ± 0.402

4.065 ± 0.327

6.756 ± 0.628

2.582 ± 0.178

7.196 ± 0.319

2.307 ± 0.519

6.647 ± 0.666

7.031 ± 1.122

8.239 ± 0.526

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.249

4.669 ± 0.743

5.301 ± 0.576

5.987 ± 0.418

6.125 ± 0.828

5.301 ± 0.48

5.932 ± 0.534

6.042 ± 0.455

2.582 ± 0.264

2.527 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski