Gorilla anellovirus
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2GN10|A0A0S2GN10_9VIRU ORF2 OS=Gorilla anellovirus OX=1743411 PE=4 SV=1
MM1 pKa = 7.63 SDD3 pKa = 2.66 RR4 pKa = 11.84 WIPPKK9 pKa = 10.45 YY10 pKa = 8.96 AWQGRR15 pKa = 11.84 EE16 pKa = 4.06 LQWINSIHH24 pKa = 5.12 TTHH27 pKa = 6.9 DD28 pKa = 3.3 TWCGCSSVITHH39 pKa = 6.95 FLRR42 pKa = 11.84 AVSARR47 pKa = 11.84 GEE49 pKa = 4.11 FLPVYY54 pKa = 9.51 TPAEE58 pKa = 4.06 KK59 pKa = 10.32 AISFLPEE66 pKa = 4.15 CPTTTEE72 pKa = 4.43 EE73 pKa = 4.4 IPPITATGDD82 pKa = 3.05 TSGIHH87 pKa = 5.09 TEE89 pKa = 4.14 EE90 pKa = 3.94 EE91 pKa = 3.97 RR92 pKa = 11.84 GLLDD96 pKa = 5.02 LEE98 pKa = 5.05 EE99 pKa = 5.57 GDD101 pKa = 4.67 SDD103 pKa = 5.23 ALFADD108 pKa = 4.04 DD109 pKa = 5.28 HH110 pKa = 7.07 EE111 pKa = 4.82 EE112 pKa = 4.15 DD113 pKa = 3.69 AAGG116 pKa = 3.58
Molecular weight: 12.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.312
IPC2_protein 4.355
IPC_protein 4.266
Toseland 4.113
ProMoST 4.38
Dawson 4.215
Bjellqvist 4.368
Wikipedia 4.101
Rodwell 4.113
Grimsley 4.024
Solomon 4.215
Lehninger 4.164
Nozaki 4.329
DTASelect 4.482
Thurlkill 4.126
EMBOSS 4.113
Sillero 4.38
Patrickios 3.376
IPC_peptide 4.215
IPC2_peptide 4.38
IPC2.peptide.svr19 4.281
Protein with the highest isoelectric point:
>tr|A0A0S2GN10|A0A0S2GN10_9VIRU ORF2 OS=Gorilla anellovirus OX=1743411 PE=4 SV=1
MM1 pKa = 7.71 PYY3 pKa = 10.06 YY4 pKa = 10.5 YY5 pKa = 10.23 RR6 pKa = 11.84 RR7 pKa = 11.84 NTPYY11 pKa = 10.05 YY12 pKa = 9.62 RR13 pKa = 11.84 YY14 pKa = 9.82 RR15 pKa = 11.84 RR16 pKa = 11.84 YY17 pKa = 9.32 FWNPYY22 pKa = 8.16 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 KK26 pKa = 10.04 RR27 pKa = 11.84 IIRR30 pKa = 11.84 PRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LRR37 pKa = 11.84 RR38 pKa = 11.84 LVRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 PRR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 WVRR52 pKa = 11.84 RR53 pKa = 11.84 KK54 pKa = 10.28 KK55 pKa = 9.62 KK56 pKa = 9.26 TIFLKK61 pKa = 9.91 QWQPPTIRR69 pKa = 11.84 RR70 pKa = 11.84 CKK72 pKa = 10.12 IKK74 pKa = 10.82 GHH76 pKa = 5.71 MCSMMCAHH84 pKa = 6.9 GNEE87 pKa = 4.12 SHH89 pKa = 7.28 NYY91 pKa = 7.24 NQYY94 pKa = 9.61 VKK96 pKa = 10.51 SYY98 pKa = 9.72 TPSNYY103 pKa = 9.49 PGGGGFSILRR113 pKa = 11.84 FSLGALYY120 pKa = 10.22 EE121 pKa = 4.13 QFSDD125 pKa = 5.85 FFNFWTKK132 pKa = 7.89 TNKK135 pKa = 9.94 NLDD138 pKa = 3.22 LCRR141 pKa = 11.84 YY142 pKa = 8.49 IKK144 pKa = 10.83 CNLLFWRR151 pKa = 11.84 HH152 pKa = 5.45 SDD154 pKa = 2.89 VSYY157 pKa = 10.54 IVSYY161 pKa = 10.39 NKK163 pKa = 10.08 NYY165 pKa = 10.12 PMTANPLSHH174 pKa = 6.59 AQAHH178 pKa = 6.59 PSRR181 pKa = 11.84 QILLKK186 pKa = 10.39 KK187 pKa = 10.27 KK188 pKa = 10.58 LIVPSFKK195 pKa = 10.57 EE196 pKa = 3.79 NRR198 pKa = 11.84 NKK200 pKa = 10.38 KK201 pKa = 8.37 PYY203 pKa = 9.94 KK204 pKa = 10.0 RR205 pKa = 11.84 LTIKK209 pKa = 10.5 PPSLLNSKK217 pKa = 8.67 WFFQKK222 pKa = 10.75 DD223 pKa = 3.44 FADD226 pKa = 3.34 VGLFLLTVTSCSLNHH241 pKa = 6.68 AFAATNAEE249 pKa = 4.3 SNNISIYY256 pKa = 10.6 CLNTQNVFKK265 pKa = 10.54 KK266 pKa = 9.94 INYY269 pKa = 5.92 VTTSGQQNYY278 pKa = 8.44 QPLGGTEE285 pKa = 4.18 TLFAASQKK293 pKa = 9.87 TNLTDD298 pKa = 3.39 NNTWPLGSQKK308 pKa = 9.0 YY309 pKa = 7.84 TEE311 pKa = 5.0 GNPPSNPGNIFFKK324 pKa = 10.54 SYY326 pKa = 11.33 ASGEE330 pKa = 4.22 TYY332 pKa = 10.06 IFKK335 pKa = 10.74 GNKK338 pKa = 8.78 PKK340 pKa = 9.91 GTEE343 pKa = 4.05 FKK345 pKa = 10.15 EE346 pKa = 3.78 PLYY349 pKa = 10.43 IEE351 pKa = 4.93 CRR353 pKa = 11.84 YY354 pKa = 10.66 NPLLDD359 pKa = 4.61 NGDD362 pKa = 3.59 GNQIYY367 pKa = 10.41 LLSTGTTSNPWQPPSDD383 pKa = 3.54 QQLIISGLPLYY394 pKa = 10.06 IALFGWTDD402 pKa = 3.88 WIRR405 pKa = 11.84 KK406 pKa = 7.91 LRR408 pKa = 11.84 PASQIDD414 pKa = 3.46 INYY417 pKa = 8.87 QLVIITDD424 pKa = 4.56 KK425 pKa = 10.27 ITPKK429 pKa = 10.42 LPAYY433 pKa = 9.66 IPISEE438 pKa = 4.27 YY439 pKa = 10.58 FIEE442 pKa = 5.39 GNGLYY447 pKa = 9.04 QQPLTDD453 pKa = 3.5 TEE455 pKa = 4.19 LAHH458 pKa = 6.91 WYY460 pKa = 9.44 PRR462 pKa = 11.84 VKK464 pKa = 10.48 FQEE467 pKa = 3.83 PAINDD472 pKa = 3.41 IVKK475 pKa = 10.15 CGPFMPRR482 pKa = 11.84 AEE484 pKa = 5.32 GIHH487 pKa = 5.97 SWEE490 pKa = 3.91 AHH492 pKa = 4.91 CTYY495 pKa = 11.24 NFIFKK500 pKa = 9.56 WGGSVPPEE508 pKa = 3.9 QDD510 pKa = 2.51 IKK512 pKa = 11.68 DD513 pKa = 4.76 PISQPAYY520 pKa = 10.12 SVPDD524 pKa = 3.72 KK525 pKa = 11.16 LSTTIQIEE533 pKa = 4.24 DD534 pKa = 3.71 PRR536 pKa = 11.84 NISPYY541 pKa = 7.95 TTLHH545 pKa = 5.88 PWDD548 pKa = 3.63 IRR550 pKa = 11.84 RR551 pKa = 11.84 GTLTEE556 pKa = 4.1 TTLKK560 pKa = 10.6 RR561 pKa = 11.84 ILEE564 pKa = 4.35 DD565 pKa = 3.72 AEE567 pKa = 4.65 TKK569 pKa = 10.54 ASCITGNEE577 pKa = 4.02 STQILTKK584 pKa = 10.13 RR585 pKa = 11.84 RR586 pKa = 11.84 RR587 pKa = 11.84 TMEE590 pKa = 4.74 PDD592 pKa = 3.78 LYY594 pKa = 10.99 TDD596 pKa = 4.35 IPWDD600 pKa = 3.44 QQLLHH605 pKa = 6.81 KK606 pKa = 10.67 AFEE609 pKa = 4.28 EE610 pKa = 4.47 NIFQEE615 pKa = 4.49 TEE617 pKa = 4.03 EE618 pKa = 4.49 EE619 pKa = 4.34 EE620 pKa = 4.44 TTQGNLIEE628 pKa = 4.4 QFNKK632 pKa = 9.86 QKK634 pKa = 10.04 QQQQRR639 pKa = 11.84 LRR641 pKa = 11.84 QQLTTVILSLKK652 pKa = 8.09 EE653 pKa = 4.05 QQHH656 pKa = 5.22 KK657 pKa = 9.16 MQMQMGTLL665 pKa = 3.51
Molecular weight: 78.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.861
IPC2_protein 9.253
IPC_protein 9.209
Toseland 9.706
ProMoST 9.531
Dawson 9.999
Bjellqvist 9.736
Wikipedia 10.204
Rodwell 10.277
Grimsley 10.101
Solomon 10.014
Lehninger 9.97
Nozaki 9.78
DTASelect 9.706
Thurlkill 9.823
EMBOSS 10.145
Sillero 9.926
Patrickios 4.978
IPC_peptide 10.014
IPC2_peptide 8.478
IPC2.peptide.svr19 8.166
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
781
116
665
390.5
45.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.353 ± 1.988
1.793 ± 0.37
4.097 ± 1.706
5.762 ± 2.537
4.353 ± 0.422
5.122 ± 0.827
2.305 ± 0.934
7.042 ± 0.068
6.274 ± 2.12
8.195 ± 0.605
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.408 ± 0.255
5.762 ± 2.283
7.042 ± 0.068
5.634 ± 1.821
6.786 ± 1.153
6.146 ± 0.35
8.067 ± 1.061
2.433 ± 0.071
2.433 ± 0.473
4.994 ± 1.523
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here