Sanxia water strider virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJ76|A0A1L3KJ76_9VIRU Uncharacterized protein OS=Sanxia water strider virus 7 OX=1923406 PE=4 SV=1
MM1 pKa = 7.82SDD3 pKa = 3.27PHH5 pKa = 7.31GDD7 pKa = 3.49PAPVDD12 pKa = 3.38RR13 pKa = 11.84GGVISVEE20 pKa = 4.13VSEE23 pKa = 4.61EE24 pKa = 4.21SSGKK28 pKa = 9.5PDD30 pKa = 3.71FPTSIILSRR39 pKa = 11.84FCSVEE44 pKa = 3.77SEE46 pKa = 4.23QVYY49 pKa = 10.9KK50 pKa = 11.07DD51 pKa = 4.5LLNTWSILPPIIWEE65 pKa = 4.3GTTDD69 pKa = 4.98AVTTYY74 pKa = 10.53NLPYY78 pKa = 10.26YY79 pKa = 10.4AVEE82 pKa = 4.13SLKK85 pKa = 10.55DD86 pKa = 3.89YY87 pKa = 10.94PQMLPFRR94 pKa = 11.84MYY96 pKa = 10.23QYY98 pKa = 9.9WRR100 pKa = 11.84GDD102 pKa = 3.12IEE104 pKa = 4.14IRR106 pKa = 11.84ATVAGSPFAIGQQQMAWYY124 pKa = 9.95YY125 pKa = 11.05DD126 pKa = 3.5ATHH129 pKa = 7.32DD130 pKa = 4.89SMFDD134 pKa = 3.21VMRR137 pKa = 11.84KK138 pKa = 9.02NKK140 pKa = 9.25YY141 pKa = 9.43ARR143 pKa = 11.84STMLHH148 pKa = 5.23TLIDD152 pKa = 4.14ASPSNDD158 pKa = 2.7AVLYY162 pKa = 10.21IPYY165 pKa = 9.99KK166 pKa = 9.72SYY168 pKa = 11.27RR169 pKa = 11.84SFLQTRR175 pKa = 11.84TTRR178 pKa = 11.84EE179 pKa = 4.03EE180 pKa = 3.62QGKK183 pKa = 8.63PLDD186 pKa = 4.74LGTLQIGVLNEE197 pKa = 3.8LLVPDD202 pKa = 5.12GATKK206 pKa = 10.1QVSLVLHH213 pKa = 5.98IRR215 pKa = 11.84FPNSEE220 pKa = 3.81FQGRR224 pKa = 11.84VSTALPGHH232 pKa = 6.95LL233 pKa = 4.33

Molecular weight: 26.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJB6|A0A1L3KJB6_9VIRU SF3 helicase domain-containing protein OS=Sanxia water strider virus 7 OX=1923406 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84FNIPDD7 pKa = 3.9FGVVADD13 pKa = 4.61IFTTLMDD20 pKa = 4.53LLEE23 pKa = 4.8DD24 pKa = 4.16PKK26 pKa = 11.17TSIEE30 pKa = 4.25DD31 pKa = 3.4KK32 pKa = 11.15VKK34 pKa = 10.49IRR36 pKa = 11.84LDD38 pKa = 3.48LLSNEE43 pKa = 3.99GAYY46 pKa = 10.49GFDD49 pKa = 3.78LPSFGATRR57 pKa = 11.84RR58 pKa = 11.84VVKK61 pKa = 10.16EE62 pKa = 3.73VVPTIISEE70 pKa = 4.39DD71 pKa = 3.58KK72 pKa = 10.96LITPVKK78 pKa = 10.27RR79 pKa = 11.84GMSYY83 pKa = 11.51ANRR86 pKa = 11.84LQQRR90 pKa = 11.84VAKK93 pKa = 8.04PVKK96 pKa = 9.98YY97 pKa = 10.21IPPNCFCPEE106 pKa = 4.16CAPKK110 pKa = 10.35NAIYY114 pKa = 10.56SSSQYY119 pKa = 10.43YY120 pKa = 9.37RR121 pKa = 11.84EE122 pKa = 3.69LWIMNEE128 pKa = 3.2ARR130 pKa = 11.84KK131 pKa = 9.81RR132 pKa = 11.84GKK134 pKa = 9.86QLDD137 pKa = 3.9LKK139 pKa = 10.25KK140 pKa = 10.8ASSQATKK147 pKa = 10.81SRR149 pKa = 11.84TDD151 pKa = 3.56FVTITSKK158 pKa = 8.19THH160 pKa = 5.03ISARR164 pKa = 11.84NKK166 pKa = 10.19QILEE170 pKa = 4.02NVLLDD175 pKa = 3.6RR176 pKa = 11.84TSKK179 pKa = 9.02KK180 pKa = 7.35TKK182 pKa = 9.88KK183 pKa = 9.3MAVKK187 pKa = 10.21EE188 pKa = 4.28EE189 pKa = 4.11IVQSVSKK196 pKa = 9.73VTAQPKK202 pKa = 10.19KK203 pKa = 9.88KK204 pKa = 10.75VEE206 pKa = 4.15INSLPSSKK214 pKa = 10.55EE215 pKa = 3.54EE216 pKa = 3.96KK217 pKa = 9.55TNSLVFVSKK226 pKa = 10.75RR227 pKa = 11.84KK228 pKa = 6.08QTRR231 pKa = 11.84NLTSFSRR238 pKa = 11.84SDD240 pKa = 3.27EE241 pKa = 4.19KK242 pKa = 10.88QSRR245 pKa = 11.84IYY247 pKa = 10.39TPIIDD252 pKa = 4.08PNYY255 pKa = 8.89KK256 pKa = 9.95PRR258 pKa = 11.84IKK260 pKa = 10.48VEE262 pKa = 3.5KK263 pKa = 10.43SGFIKK268 pKa = 10.67SRR270 pKa = 11.84TVNNAKK276 pKa = 8.79TVEE279 pKa = 4.07ITPFLAKK286 pKa = 10.48LPPQVVNSTSEE297 pKa = 4.13TVSVKK302 pKa = 10.19KK303 pKa = 9.89VQPEE307 pKa = 4.19PEE309 pKa = 4.35TPKK312 pKa = 10.57QPNNRR317 pKa = 11.84IRR319 pKa = 11.84TKK321 pKa = 10.51LIRR324 pKa = 11.84EE325 pKa = 3.82FRR327 pKa = 11.84EE328 pKa = 4.19LIRR331 pKa = 11.84VSGLKK336 pKa = 9.25GTFSKK341 pKa = 10.6EE342 pKa = 3.77GKK344 pKa = 6.29PTKK347 pKa = 10.01DD348 pKa = 2.8TVLVLDD354 pKa = 4.68ALISDD359 pKa = 3.98MRR361 pKa = 11.84IKK363 pKa = 10.77ADD365 pKa = 3.4QLKK368 pKa = 10.48VSPEE372 pKa = 4.19SINLKK377 pKa = 8.95STLQSTLGTAFFVQSLNVV395 pKa = 3.25

Molecular weight: 44.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3033

233

2405

1011.0

114.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.341 ± 0.255

2.275 ± 0.815

5.935 ± 0.53

5.308 ± 0.254

5.44 ± 0.884

4.946 ± 0.693

1.945 ± 0.569

6.924 ± 0.344

6.034 ± 1.884

7.946 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.238

4.286 ± 0.39

5.671 ± 0.472

3.825 ± 0.261

5.176 ± 0.397

9.034 ± 0.201

5.869 ± 0.812

6.462 ± 0.7

1.352 ± 0.356

4.055 ± 0.659
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski