Marinobacter phage AS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2YTR5|A0A3G2YTR5_9CAUD Uncharacterized protein OS=Marinobacter phage AS1 OX=2484222 GN=AS1_8 PE=4 SV=1
MM1 pKa = 7.69ISYY4 pKa = 9.86CPSYY8 pKa = 11.5VEE10 pKa = 4.07VLKK13 pKa = 9.67EE14 pKa = 4.01TIEE17 pKa = 4.17ACNDD21 pKa = 3.08DD22 pKa = 4.11KK23 pKa = 11.94ADD25 pKa = 3.57VRR27 pKa = 11.84SWDD30 pKa = 3.24QSQAQKK36 pKa = 10.68LQEE39 pKa = 4.15LNEE42 pKa = 4.09

Molecular weight: 4.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2YTK5|A0A3G2YTK5_9CAUD Uncharacterized protein OS=Marinobacter phage AS1 OX=2484222 GN=AS1_34 PE=4 SV=1
MM1 pKa = 7.6HH2 pKa = 7.87LDD4 pKa = 3.32LPEE7 pKa = 3.89NTRR10 pKa = 11.84VIDD13 pKa = 4.74LIRR16 pKa = 11.84FANAQGKK23 pKa = 9.02RR24 pKa = 11.84LVWKK28 pKa = 10.62QEE30 pKa = 3.54GFRR33 pKa = 11.84YY34 pKa = 9.47RR35 pKa = 11.84AHH37 pKa = 7.85LEE39 pKa = 3.7EE40 pKa = 4.9AANDD44 pKa = 3.56QPPVVTRR51 pKa = 11.84LRR53 pKa = 11.84PRR55 pKa = 11.84LRR57 pKa = 11.84VVDD60 pKa = 4.07SQKK63 pKa = 11.01KK64 pKa = 8.85

Molecular weight: 7.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11711

42

1072

225.2

24.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.016 ± 0.453

0.837 ± 0.148

6.208 ± 0.198

6.899 ± 0.438

3.04 ± 0.166

7.207 ± 0.363

1.614 ± 0.213

5.414 ± 0.211

4.867 ± 0.445

8.445 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.058 ± 0.235

3.783 ± 0.232

4.671 ± 0.378

4.773 ± 0.266

7.164 ± 0.42

6.421 ± 0.279

6.14 ± 0.539

6.319 ± 0.3

1.58 ± 0.154

2.545 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski