Bovine serum-associated circular virus
Average proteome isoelectric point is 8.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385MJ33|A0A385MJ33_9VIRU Uncharacterized protein OS=Bovine serum-associated circular virus OX=2340909 PE=4 SV=1
MM1 pKa = 7.22 PRR3 pKa = 11.84 ARR5 pKa = 11.84 RR6 pKa = 11.84 TFYY9 pKa = 9.23 TARR12 pKa = 11.84 RR13 pKa = 11.84 QTVKK17 pKa = 10.18 WSPHH21 pKa = 5.76 LLIQPMTMEE30 pKa = 4.53 ATQGEE35 pKa = 4.6 FASYY39 pKa = 10.81 QLIAKK44 pKa = 9.32 NSSDD48 pKa = 3.03 TSIPVPSVIKK58 pKa = 10.5 VKK60 pKa = 10.5 NFKK63 pKa = 10.8 VSIDD67 pKa = 3.41 TSAPSDD73 pKa = 3.9 FSQGMKK79 pKa = 10.32 YY80 pKa = 10.6 LNAYY84 pKa = 9.49 LMYY87 pKa = 9.61 VPEE90 pKa = 5.65 GITLNTDD97 pKa = 3.24 TAKK100 pKa = 10.61 NHH102 pKa = 6.36 PEE104 pKa = 3.8 WIIAWRR110 pKa = 11.84 GINDD114 pKa = 3.69 TNQSDD119 pKa = 3.44 RR120 pKa = 11.84 DD121 pKa = 3.88 NYY123 pKa = 10.34 VLSSRR128 pKa = 11.84 LARR131 pKa = 11.84 NLNSGDD137 pKa = 3.88 AIYY140 pKa = 11.04 LLFAGYY146 pKa = 8.86 TNTTHH151 pKa = 6.7 SFSFVATTTYY161 pKa = 10.63 VSRR164 pKa = 11.84 ANN166 pKa = 3.38
Molecular weight: 18.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.819
IPC2_protein 9.165
IPC_protein 9.224
Toseland 9.428
ProMoST 9.341
Dawson 9.765
Bjellqvist 9.516
Wikipedia 10.014
Rodwell 9.897
Grimsley 9.897
Solomon 9.823
Lehninger 9.765
Nozaki 9.385
DTASelect 9.516
Thurlkill 9.56
EMBOSS 9.867
Sillero 9.677
Patrickios 4.558
IPC_peptide 9.809
IPC2_peptide 8.185
IPC2.peptide.svr19 7.964
Protein with the highest isoelectric point:
>tr|A0A385MJ33|A0A385MJ33_9VIRU Uncharacterized protein OS=Bovine serum-associated circular virus OX=2340909 PE=4 SV=1
MM1 pKa = 7.22 PRR3 pKa = 11.84 ARR5 pKa = 11.84 RR6 pKa = 11.84 TFYY9 pKa = 9.23 TARR12 pKa = 11.84 RR13 pKa = 11.84 QTVKK17 pKa = 10.18 WSPHH21 pKa = 5.76 LLIQPMTMEE30 pKa = 4.53 ATQGEE35 pKa = 4.6 FASYY39 pKa = 10.81 QLIAKK44 pKa = 9.32 NSSDD48 pKa = 3.03 TSIPVPSVIKK58 pKa = 10.5 VKK60 pKa = 10.5 NFKK63 pKa = 10.8 VSIDD67 pKa = 3.41 TSAPSDD73 pKa = 3.9 FSQGMKK79 pKa = 10.32 YY80 pKa = 10.6 LNAYY84 pKa = 9.49 LMYY87 pKa = 9.61 VPEE90 pKa = 5.65 GITLNTDD97 pKa = 3.24 TAKK100 pKa = 10.61 NHH102 pKa = 6.36 PEE104 pKa = 3.8 WIIAWRR110 pKa = 11.84 GINDD114 pKa = 3.69 TNQSDD119 pKa = 3.44 RR120 pKa = 11.84 DD121 pKa = 3.88 NYY123 pKa = 10.34 VLSSRR128 pKa = 11.84 LARR131 pKa = 11.84 NLNSGDD137 pKa = 3.88 AIYY140 pKa = 11.04 LLFAGYY146 pKa = 8.86 TNTTHH151 pKa = 6.7 SFSFVATTTYY161 pKa = 10.63 VSRR164 pKa = 11.84 ANN166 pKa = 3.38
Molecular weight: 18.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.819
IPC2_protein 9.165
IPC_protein 9.224
Toseland 9.428
ProMoST 9.341
Dawson 9.765
Bjellqvist 9.516
Wikipedia 10.014
Rodwell 9.897
Grimsley 9.897
Solomon 9.823
Lehninger 9.765
Nozaki 9.385
DTASelect 9.516
Thurlkill 9.56
EMBOSS 9.867
Sillero 9.677
Patrickios 4.558
IPC_peptide 9.809
IPC2_peptide 8.185
IPC2.peptide.svr19 7.964
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
166
166
166
166.0
18.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.434 ± 0.0
0.0 ± 0.0
4.819 ± 0.0
2.41 ± 0.0
4.217 ± 0.0
3.614 ± 0.0
1.807 ± 0.0
6.024 ± 0.0
4.217 ± 0.0
6.627 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.012 ± 0.0
7.229 ± 0.0
4.819 ± 0.0
3.614 ± 0.0
6.024 ± 0.0
10.241 ± 0.0
10.241 ± 0.0
5.422 ± 0.0
1.807 ± 0.0
5.422 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here