Buchnera aphidicola (Sarucallis kahawaluokalani)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola

Average proteome isoelectric point is 8.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 392 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6YJ66|A0A4D6YJ66_9GAMM NADH dehydrogenase I subunit M OS=Buchnera aphidicola (Sarucallis kahawaluokalani) OX=1241878 GN=D9V78_00595 PE=3 SV=1
MM1 pKa = 7.6SVEE4 pKa = 4.18NYY6 pKa = 10.67NFFLFVVCVLIMCGMMLLGGWLLGQRR32 pKa = 11.84GVSRR36 pKa = 11.84GKK38 pKa = 10.2NIPFEE43 pKa = 4.31SGINSYY49 pKa = 11.45GDD51 pKa = 3.48ANVRR55 pKa = 11.84WVIKK59 pKa = 10.12FYY61 pKa = 11.03LLGVFFVIFDD71 pKa = 3.74VEE73 pKa = 4.22SLYY76 pKa = 10.93LYY78 pKa = 9.59TWIINIKK85 pKa = 9.39NIGWFGFFEE94 pKa = 3.86ISLFIFTIFLALFYY108 pKa = 10.73LLKK111 pKa = 10.85EE112 pKa = 4.34NVFNWDD118 pKa = 3.24SCNDD122 pKa = 4.1DD123 pKa = 3.96III125 pKa = 6.23

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6YIM6|A0A4D6YIM6_9GAMM Preprotein translocase subunit SecE OS=Buchnera aphidicola (Sarucallis kahawaluokalani) OX=1241878 GN=secE PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.52RR12 pKa = 11.84NRR14 pKa = 11.84VHH16 pKa = 7.36GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84SGRR28 pKa = 11.84QILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 10.2LRR39 pKa = 11.84IRR41 pKa = 11.84LTVSTHH47 pKa = 4.58MKK49 pKa = 10.21

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

392

0

392

125482

38

1383

320.1

36.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.142 ± 0.109

1.387 ± 0.043

4.027 ± 0.07

4.233 ± 0.098

4.946 ± 0.121

5.208 ± 0.118

2.557 ± 0.052

13.077 ± 0.156

10.041 ± 0.16

9.352 ± 0.103

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.055

7.567 ± 0.12

2.965 ± 0.056

3.809 ± 0.065

3.356 ± 0.092

6.14 ± 0.079

4.944 ± 0.07

4.719 ± 0.103

0.863 ± 0.048

4.315 ± 0.086
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski