Ensifer sp. MPMI2T

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Ensifer; unclassified Ensifer

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6911 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S5JCB4|A0A4S5JCB4_9RHIZ Cytochrome c oxidase subunit 3 family protein OS=Ensifer sp. MPMI2T OX=2490847 GN=EHS39_12555 PE=3 SV=1
MM1 pKa = 7.44ATRR4 pKa = 11.84IYY6 pKa = 8.54GTNYY10 pKa = 9.5SDD12 pKa = 5.67KK13 pKa = 10.26IVQNGYY19 pKa = 10.13ISVEE23 pKa = 3.81IYY25 pKa = 11.03ALDD28 pKa = 4.18GNDD31 pKa = 3.53QIHH34 pKa = 7.03LNRR37 pKa = 11.84TDD39 pKa = 3.22SYY41 pKa = 11.54GGYY44 pKa = 9.83NFVDD48 pKa = 3.03AGYY51 pKa = 11.09GNDD54 pKa = 3.06VVVNYY59 pKa = 9.75FEE61 pKa = 4.84GGNDD65 pKa = 2.82IFMGAGDD72 pKa = 4.81DD73 pKa = 5.36LYY75 pKa = 10.78IADD78 pKa = 4.12ARR80 pKa = 11.84VRR82 pKa = 11.84DD83 pKa = 3.73ASSYY87 pKa = 9.79DD88 pKa = 3.43VVYY91 pKa = 10.64GGSGNDD97 pKa = 2.84RR98 pKa = 11.84FEE100 pKa = 5.38IEE102 pKa = 4.72TYY104 pKa = 10.89ASDD107 pKa = 4.2YY108 pKa = 11.31YY109 pKa = 11.33GDD111 pKa = 4.02SGNDD115 pKa = 3.1TFLSVGFKK123 pKa = 10.85NYY125 pKa = 10.11FNGGTGIDD133 pKa = 3.73TISYY137 pKa = 9.68QLQDD141 pKa = 2.92SFGSEE146 pKa = 3.75RR147 pKa = 11.84GRR149 pKa = 11.84GVTIDD154 pKa = 4.24LGYY157 pKa = 10.82KK158 pKa = 9.79YY159 pKa = 9.96ATTGTGRR166 pKa = 11.84RR167 pKa = 11.84EE168 pKa = 3.75DD169 pKa = 4.38LISIEE174 pKa = 4.21NATGTNYY181 pKa = 10.62GNDD184 pKa = 4.77DD185 pKa = 3.4ITGSSVANVLRR196 pKa = 11.84GLGGHH201 pKa = 7.53DD202 pKa = 3.09ILEE205 pKa = 4.52GLGGDD210 pKa = 4.31DD211 pKa = 5.01FLDD214 pKa = 4.38GGSGDD219 pKa = 4.1DD220 pKa = 4.32DD221 pKa = 5.55LYY223 pKa = 11.4GGSGADD229 pKa = 3.28ILRR232 pKa = 11.84GGTGFDD238 pKa = 3.83YY239 pKa = 11.39LNGGTGTDD247 pKa = 3.1SFDD250 pKa = 3.78FNSISEE256 pKa = 4.45SAVGSRR262 pKa = 11.84RR263 pKa = 11.84DD264 pKa = 3.62VIADD268 pKa = 3.52FHH270 pKa = 7.27RR271 pKa = 11.84SEE273 pKa = 4.18WDD275 pKa = 3.64VIDD278 pKa = 5.72LSTIDD283 pKa = 6.04ADD285 pKa = 4.42TTWSGNQSFTFIGSRR300 pKa = 11.84GFSGEE305 pKa = 3.84AGEE308 pKa = 4.73LNFRR312 pKa = 11.84YY313 pKa = 9.94GVVSGDD319 pKa = 3.45INGDD323 pKa = 3.44GYY325 pKa = 11.79ADD327 pKa = 3.67FQIKK331 pKa = 10.08VNGVTSLRR339 pKa = 11.84VDD341 pKa = 3.74DD342 pKa = 5.5FFLL345 pKa = 5.77

Molecular weight: 37.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S5JEC3|A0A4S5JEC3_9RHIZ Proline/glycine betaine ABC transporter permease OS=Ensifer sp. MPMI2T OX=2490847 GN=EHS39_00570 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6911

0

6911

2097564

28

3083

303.5

33.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.988 ± 0.036

0.862 ± 0.008

5.607 ± 0.023

6.059 ± 0.027

3.905 ± 0.018

8.241 ± 0.03

2.084 ± 0.015

5.494 ± 0.019

3.556 ± 0.024

9.951 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.012

2.789 ± 0.016

4.95 ± 0.021

3.022 ± 0.017

7.118 ± 0.032

5.688 ± 0.023

5.224 ± 0.022

7.356 ± 0.022

1.324 ± 0.011

2.314 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski