Pseudomonas fulva (strain 12-X)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas putida group; Pseudomonas fulva

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4459 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6AER3|F6AER3_PSEF1 (R)-benzylsuccinyl-CoA dehydrogenase OS=Pseudomonas fulva (strain 12-X) OX=743720 GN=Psefu_2524 PE=3 SV=1
MM1 pKa = 7.14TFVVTDD7 pKa = 3.25NCIKK11 pKa = 10.54CKK13 pKa = 10.0YY14 pKa = 7.47TDD16 pKa = 3.85CVEE19 pKa = 4.15VCPVDD24 pKa = 4.13CFYY27 pKa = 11.12EE28 pKa = 4.9GPNFLVIHH36 pKa = 6.99PDD38 pKa = 3.12EE39 pKa = 5.91CIDD42 pKa = 4.16CALCEE47 pKa = 4.36PEE49 pKa = 5.63CPAQAIFSEE58 pKa = 4.65DD59 pKa = 4.04EE60 pKa = 4.35IPDD63 pKa = 3.47GMQEE67 pKa = 4.12YY68 pKa = 10.46IEE70 pKa = 5.0LNAEE74 pKa = 4.17LAEE77 pKa = 4.05VWPNITEE84 pKa = 4.17RR85 pKa = 11.84KK86 pKa = 8.41EE87 pKa = 3.94ALPDD91 pKa = 3.47AEE93 pKa = 4.48EE94 pKa = 4.01WDD96 pKa = 4.01GVKK99 pKa = 10.7DD100 pKa = 3.74KK101 pKa = 11.02LQHH104 pKa = 6.41LEE106 pKa = 3.86RR107 pKa = 5.99

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6ABN1|F6ABN1_PSEF1 Extracellular solute-binding protein family 5 OS=Pseudomonas fulva (strain 12-X) OX=743720 GN=Psefu_2256 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.31GRR39 pKa = 11.84ARR41 pKa = 11.84LTVV44 pKa = 3.04

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4459

0

4459

1450635

31

5320

325.3

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.477 ± 0.06

0.961 ± 0.013

5.37 ± 0.032

5.859 ± 0.044

3.551 ± 0.023

8.022 ± 0.04

2.189 ± 0.022

4.763 ± 0.029

3.262 ± 0.035

11.93 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.018

2.874 ± 0.03

4.791 ± 0.033

4.631 ± 0.036

6.622 ± 0.054

5.724 ± 0.03

4.732 ± 0.072

7.119 ± 0.031

1.394 ± 0.015

2.432 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski