Candidatus Kinetoplastibacterium crithidii TCC036E

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Kinetoplastibacterium; Candidatus Kinetoplastibacterium crithidii

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 728 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1LU35|M1LU35_9PROT OmpH-like outer membrane chaperone OS=Candidatus Kinetoplastibacterium crithidii TCC036E OX=1208918 GN=CDEE_0588 PE=3 SV=1
MM1 pKa = 7.1QFCDD5 pKa = 3.75QEE7 pKa = 4.26ILVDD11 pKa = 3.87VSTAAIDD18 pKa = 3.37TGGYY22 pKa = 10.42GVFLTVTSEE31 pKa = 3.96GGTEE35 pKa = 3.69VDD37 pKa = 4.21NVFSYY42 pKa = 10.98LGDD45 pKa = 3.73CASLFEE51 pKa = 4.52ARR53 pKa = 11.84NLAEE57 pKa = 4.13IFAKK61 pKa = 10.17KK62 pKa = 9.63WIDD65 pKa = 3.48EE66 pKa = 4.54NIFPP70 pKa = 5.56

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1LTD5|M1LTD5_9PROT Primosomal protein N' OS=Candidatus Kinetoplastibacterium crithidii TCC036E OX=1208918 GN=priA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.08QPSVLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.44RR14 pKa = 11.84THH16 pKa = 6.01GFRR19 pKa = 11.84IRR21 pKa = 11.84MKK23 pKa = 9.01TRR25 pKa = 11.84SGRR28 pKa = 11.84AIINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.51GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

728

0

728

244635

38

1816

336.0

37.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.703 ± 0.083

1.206 ± 0.026

5.516 ± 0.056

5.833 ± 0.077

4.517 ± 0.077

6.051 ± 0.086

1.981 ± 0.035

10.118 ± 0.087

7.401 ± 0.079

9.814 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.04

6.549 ± 0.08

3.3 ± 0.041

2.857 ± 0.044

4.25 ± 0.059

8.006 ± 0.072

4.416 ± 0.051

5.958 ± 0.084

0.945 ± 0.033

3.318 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski