Amino acid dipepetide frequency for Pelomonas sp. HMWF004

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.776AlaAla: 19.776 ± 0.18	1.389AlaCys: 1.389 ± 0.036	6.808AlaAsp: 6.808 ± 0.066	7.341AlaGlu: 7.341 ± 0.076	4.035AlaPhe: 4.035 ± 0.064	11.551AlaGly: 11.551 ± 0.114	2.609AlaHis: 2.609 ± 0.046	4.525AlaIle: 4.525 ± 0.055	3.877AlaLys: 3.877 ± 0.064	16.176AlaLeu: 16.176 ± 0.141	3.239AlaMet: 3.239 ± 0.055	3.062AlaAsn: 3.062 ± 0.06	6.407AlaPro: 6.407 ± 0.086	6.407AlaGln: 6.407 ± 0.085	9.114AlaArg: 9.114 ± 0.094	7.326AlaSer: 7.326 ± 0.085	6.595AlaThr: 6.595 ± 0.088	9.334AlaVal: 9.334 ± 0.087	2.278AlaTrp: 2.278 ± 0.044	2.62AlaTyr: 2.62 ± 0.055	0.011AlaXaa: 0.011 ± 0.003
Cys	1.127CysAla: 1.127 ± 0.03	0.131CysCys: 0.131 ± 0.01	0.526CysAsp: 0.526 ± 0.02	0.479CysGlu: 0.479 ± 0.02	0.291CysPhe: 0.291 ± 0.015	0.952CysGly: 0.952 ± 0.032	0.262CysHis: 0.262 ± 0.014	0.366CysIle: 0.366 ± 0.014	0.238CysLys: 0.238 ± 0.013	0.853CysLeu: 0.853 ± 0.024	0.177CysMet: 0.177 ± 0.011	0.23CysAsn: 0.23 ± 0.013	0.488CysPro: 0.488 ± 0.021	0.295CysGln: 0.295 ± 0.014	0.622CysArg: 0.622 ± 0.023	0.528CysSer: 0.528 ± 0.022	0.5CysThr: 0.5 ± 0.021	0.698CysVal: 0.698 ± 0.025	0.139CysTrp: 0.139 ± 0.01	0.177CysTyr: 0.177 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.364AspAla: 7.364 ± 0.078	0.493AspCys: 0.493 ± 0.021	2.771AspAsp: 2.771 ± 0.05	3.129AspGlu: 3.129 ± 0.061	2.013AspPhe: 2.013 ± 0.038	4.931AspGly: 4.931 ± 0.077	1.048AspHis: 1.048 ± 0.034	2.252AspIle: 2.252 ± 0.043	1.647AspLys: 1.647 ± 0.043	5.366AspLeu: 5.366 ± 0.056	1.103AspMet: 1.103 ± 0.028	1.265AspAsn: 1.265 ± 0.035	2.842AspPro: 2.842 ± 0.045	1.622AspGln: 1.622 ± 0.034	3.298AspArg: 3.298 ± 0.058	2.442AspSer: 2.442 ± 0.047	2.657AspThr: 2.657 ± 0.054	3.794AspVal: 3.794 ± 0.056	1.086AspTrp: 1.086 ± 0.029	1.359AspTyr: 1.359 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	7.194GluAla: 7.194 ± 0.08	0.346GluCys: 0.346 ± 0.016	2.01GluAsp: 2.01 ± 0.048	1.972GluGlu: 1.972 ± 0.044	1.694GluPhe: 1.694 ± 0.038	3.733GluGly: 3.733 ± 0.045	1.292GluHis: 1.292 ± 0.034	2.269GluIle: 2.269 ± 0.038	1.437GluLys: 1.437 ± 0.038	6.125GluLeu: 6.125 ± 0.079	1.112GluMet: 1.112 ± 0.028	1.027GluAsn: 1.027 ± 0.029	2.604GluPro: 2.604 ± 0.047	2.656GluGln: 2.656 ± 0.047	4.511GluArg: 4.511 ± 0.065	2.247GluSer: 2.247 ± 0.038	2.215GluThr: 2.215 ± 0.041	3.939GluVal: 3.939 ± 0.055	0.687GluTrp: 0.687 ± 0.023	0.872GluTyr: 0.872 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.088PheAla: 4.088 ± 0.055	0.337PheCys: 0.337 ± 0.015	2.526PheAsp: 2.526 ± 0.046	2.024PheGlu: 2.024 ± 0.043	1.245PhePhe: 1.245 ± 0.036	3.38PheGly: 3.38 ± 0.05	0.72PheHis: 0.72 ± 0.023	1.43PheIle: 1.43 ± 0.032	1.265PheLys: 1.265 ± 0.033	2.648PheLeu: 2.648 ± 0.047	0.798PheMet: 0.798 ± 0.026	1.163PheAsn: 1.163 ± 0.032	1.321PhePro: 1.321 ± 0.032	1.055PheGln: 1.055 ± 0.025	1.703PheArg: 1.703 ± 0.032	2.137PheSer: 2.137 ± 0.036	1.939PheThr: 1.939 ± 0.036	2.512PheVal: 2.512 ± 0.041	0.545PheTrp: 0.545 ± 0.021	0.848PheTyr: 0.848 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	9.416GlyAla: 9.416 ± 0.093	0.914GlyCys: 0.914 ± 0.03	4.124GlyAsp: 4.124 ± 0.055	4.33GlyGlu: 4.33 ± 0.056	3.293GlyPhe: 3.293 ± 0.047	7.28GlyGly: 7.28 ± 0.101	1.916GlyHis: 1.916 ± 0.042	3.389GlyIle: 3.389 ± 0.057	2.904GlyLys: 2.904 ± 0.051	9.812GlyLeu: 9.812 ± 0.093	2.181GlyMet: 2.181 ± 0.042	2.267GlyAsn: 2.267 ± 0.058	3.203GlyPro: 3.203 ± 0.048	3.955GlyGln: 3.955 ± 0.05	5.937GlyArg: 5.937 ± 0.079	4.863GlySer: 4.863 ± 0.067	4.483GlyThr: 4.483 ± 0.07	6.415GlyVal: 6.415 ± 0.071	1.692GlyTrp: 1.692 ± 0.041	2.189GlyTyr: 2.189 ± 0.039	0.01GlyXaa: 0.01 ± 0.003
His	2.914HisAla: 2.914 ± 0.057	0.275HisCys: 0.275 ± 0.015	1.158HisAsp: 1.158 ± 0.028	1.171HisGlu: 1.171 ± 0.031	0.861HisPhe: 0.861 ± 0.027	2.026HisGly: 2.026 ± 0.047	0.657HisHis: 0.657 ± 0.029	0.818HisIle: 0.818 ± 0.026	0.51HisLys: 0.51 ± 0.021	2.383HisLeu: 2.383 ± 0.042	0.427HisMet: 0.427 ± 0.018	0.501HisAsn: 0.501 ± 0.019	1.323HisPro: 1.323 ± 0.03	0.756HisGln: 0.756 ± 0.024	1.553HisArg: 1.553 ± 0.041	1.061HisSer: 1.061 ± 0.029	0.967HisThr: 0.967 ± 0.03	1.487HisVal: 1.487 ± 0.031	0.454HisTrp: 0.454 ± 0.019	0.564HisTyr: 0.564 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.462IleAla: 5.462 ± 0.066	0.331IleCys: 0.331 ± 0.016	2.685IleAsp: 2.685 ± 0.048	2.67IleGlu: 2.67 ± 0.046	1.042IlePhe: 1.042 ± 0.032	3.62IleGly: 3.62 ± 0.059	0.778IleHis: 0.778 ± 0.026	1.147IleIle: 1.147 ± 0.031	1.447IleLys: 1.447 ± 0.041	2.612IleLeu: 2.612 ± 0.051	0.571IleMet: 0.571 ± 0.02	1.334IleAsn: 1.334 ± 0.031	1.664IlePro: 1.664 ± 0.038	1.304IleGln: 1.304 ± 0.034	2.195IleArg: 2.195 ± 0.042	2.275IleSer: 2.275 ± 0.042	2.381IleThr: 2.381 ± 0.039	2.933IleVal: 2.933 ± 0.051	0.425IleTrp: 0.425 ± 0.017	0.852IleTyr: 0.852 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.258LysAla: 4.258 ± 0.068	0.145LysCys: 0.145 ± 0.011	1.528LysAsp: 1.528 ± 0.042	1.216LysGlu: 1.216 ± 0.035	0.908LysPhe: 0.908 ± 0.025	2.263LysGly: 2.263 ± 0.049	0.617LysHis: 0.617 ± 0.019	1.199LysIle: 1.199 ± 0.031	1.232LysLys: 1.232 ± 0.042	3.601LysLeu: 3.601 ± 0.056	0.663LysMet: 0.663 ± 0.023	0.843LysAsn: 0.843 ± 0.029	2.128LysPro: 2.128 ± 0.041	1.154LysGln: 1.154 ± 0.033	2.252LysArg: 2.252 ± 0.043	1.625LysSer: 1.625 ± 0.039	1.811LysThr: 1.811 ± 0.043	2.292LysVal: 2.292 ± 0.05	0.308LysTrp: 0.308 ± 0.014	0.579LysTyr: 0.579 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	16.436LeuAla: 16.436 ± 0.161	1.019LeuCys: 1.019 ± 0.026	6.242LeuAsp: 6.242 ± 0.078	4.503LeuGlu: 4.503 ± 0.063	3.247LeuPhe: 3.247 ± 0.058	9.054LeuGly: 9.054 ± 0.082	2.403LeuHis: 2.403 ± 0.041	4.09LeuIle: 4.09 ± 0.056	3.81LeuLys: 3.81 ± 0.056	12.221LeuLeu: 12.221 ± 0.135	2.679LeuMet: 2.679 ± 0.049	2.958LeuAsn: 2.958 ± 0.051	6.545LeuPro: 6.545 ± 0.074	4.775LeuGln: 4.775 ± 0.056	8.21LeuArg: 8.21 ± 0.087	6.588LeuSer: 6.588 ± 0.067	6.121LeuThr: 6.121 ± 0.07	7.655LeuVal: 7.655 ± 0.083	1.531LeuTrp: 1.531 ± 0.039	2.014LeuTyr: 2.014 ± 0.038	0.007LeuXaa: 0.007 ± 0.002
Met	3.045MetAla: 3.045 ± 0.053	0.16MetCys: 0.16 ± 0.011	1.066MetAsp: 1.066 ± 0.03	0.856MetGlu: 0.856 ± 0.027	0.647MetPhe: 0.647 ± 0.024	1.727MetGly: 1.727 ± 0.041	0.513MetHis: 0.513 ± 0.019	0.776MetIle: 0.776 ± 0.024	0.952MetLys: 0.952 ± 0.028	2.498MetLeu: 2.498 ± 0.054	0.494MetMet: 0.494 ± 0.02	0.779MetAsn: 0.779 ± 0.023	1.487MetPro: 1.487 ± 0.029	1.085MetGln: 1.085 ± 0.03	1.634MetArg: 1.634 ± 0.034	1.519MetSer: 1.519 ± 0.03	1.481MetThr: 1.481 ± 0.031	1.488MetVal: 1.488 ± 0.031	0.221MetTrp: 0.221 ± 0.012	0.33MetTyr: 0.33 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.461AsnAla: 3.461 ± 0.055	0.254AsnCys: 0.254 ± 0.013	1.36AsnAsp: 1.36 ± 0.038	1.127AsnGlu: 1.127 ± 0.033	0.966AsnPhe: 0.966 ± 0.029	2.383AsnGly: 2.383 ± 0.058	0.509AsnHis: 0.509 ± 0.018	1.069AsnIle: 1.069 ± 0.031	0.757AsnLys: 0.757 ± 0.026	2.725AsnLeu: 2.725 ± 0.05	0.505AsnMet: 0.505 ± 0.02	0.829AsnAsn: 0.829 ± 0.028	1.803AsnPro: 1.803 ± 0.035	0.923AsnGln: 0.923 ± 0.03	1.577AsnArg: 1.577 ± 0.04	1.332AsnSer: 1.332 ± 0.038	1.52AsnThr: 1.52 ± 0.04	1.845AsnVal: 1.845 ± 0.044	0.428AsnTrp: 0.428 ± 0.021	0.708AsnTyr: 0.708 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	7.563ProAla: 7.563 ± 0.099	0.374ProCys: 0.374 ± 0.016	3.044ProAsp: 3.044 ± 0.048	3.252ProGlu: 3.252 ± 0.047	1.711ProPhe: 1.711 ± 0.033	4.885ProGly: 4.885 ± 0.062	1.1ProHis: 1.1 ± 0.031	1.591ProIle: 1.591 ± 0.039	1.538ProLys: 1.538 ± 0.037	5.651ProLeu: 5.651 ± 0.069	1.207ProMet: 1.207 ± 0.033	1.246ProAsn: 1.246 ± 0.035	2.859ProPro: 2.859 ± 0.062	2.214ProGln: 2.214 ± 0.037	3.123ProArg: 3.123 ± 0.058	2.804ProSer: 2.804 ± 0.05	2.68ProThr: 2.68 ± 0.042	4.129ProVal: 4.129 ± 0.052	0.869ProTrp: 0.869 ± 0.028	1.056ProTyr: 1.056 ± 0.026	0.001ProXaa: 0.001 ± 0.001
Gln	6.588GlnAla: 6.588 ± 0.091	0.258GlnCys: 0.258 ± 0.013	1.716GlnAsp: 1.716 ± 0.036	1.444GlnGlu: 1.444 ± 0.033	1.266GlnPhe: 1.266 ± 0.031	3.368GlnGly: 3.368 ± 0.056	1.002GlnHis: 1.002 ± 0.031	1.635GlnIle: 1.635 ± 0.045	0.87GlnLys: 0.87 ± 0.027	5.06GlnLeu: 5.06 ± 0.075	0.963GlnMet: 0.963 ± 0.029	0.797GlnAsn: 0.797 ± 0.027	2.71GlnPro: 2.71 ± 0.048	2.14GlnGln: 2.14 ± 0.047	3.92GlnArg: 3.92 ± 0.056	2.007GlnSer: 2.007 ± 0.044	2.029GlnThr: 2.029 ± 0.04	3.264GlnVal: 3.264 ± 0.049	0.674GlnTrp: 0.674 ± 0.022	0.736GlnTyr: 0.736 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.112ArgAla: 8.112 ± 0.086	0.646ArgCys: 0.646 ± 0.024	3.66ArgAsp: 3.66 ± 0.054	4.112ArgGlu: 4.112 ± 0.066	2.786ArgPhe: 2.786 ± 0.049	4.733ArgGly: 4.733 ± 0.058	1.863ArgHis: 1.863 ± 0.039	3.025ArgIle: 3.025 ± 0.054	1.837ArgLys: 1.837 ± 0.042	8.608ArgLeu: 8.608 ± 0.101	1.749ArgMet: 1.749 ± 0.033	1.726ArgAsn: 1.726 ± 0.041	3.378ArgPro: 3.378 ± 0.052	3.066ArgGln: 3.066 ± 0.051	5.33ArgArg: 5.33 ± 0.077	3.694ArgSer: 3.694 ± 0.056	3.078ArgThr: 3.078 ± 0.051	5.198ArgVal: 5.198 ± 0.066	1.419ArgTrp: 1.419 ± 0.036	1.869ArgTyr: 1.869 ± 0.04	0.006ArgXaa: 0.006 ± 0.002
Ser	6.888SerAla: 6.888 ± 0.08	0.496SerCys: 0.496 ± 0.021	2.588SerAsp: 2.588 ± 0.045	2.392SerGlu: 2.392 ± 0.041	2.182SerPhe: 2.182 ± 0.043	5.332SerGly: 5.332 ± 0.064	1.18SerHis: 1.18 ± 0.03	2.127SerIle: 2.127 ± 0.041	1.563SerLys: 1.563 ± 0.036	6.144SerLeu: 6.144 ± 0.073	1.224SerMet: 1.224 ± 0.031	1.494SerAsn: 1.494 ± 0.045	2.977SerPro: 2.977 ± 0.049	2.002SerGln: 2.002 ± 0.044	3.631SerArg: 3.631 ± 0.055	3.23SerSer: 3.23 ± 0.057	3.084SerThr: 3.084 ± 0.058	3.959SerVal: 3.959 ± 0.055	0.922SerTrp: 0.922 ± 0.026	1.336SerTyr: 1.336 ± 0.034	0.001SerXaa: 0.001 ± 0.001
Thr	6.812ThrAla: 6.812 ± 0.082	0.386ThrCys: 0.386 ± 0.019	2.594ThrAsp: 2.594 ± 0.051	2.423ThrGlu: 2.423 ± 0.043	1.455ThrPhe: 1.455 ± 0.035	4.882ThrGly: 4.882 ± 0.069	1.1ThrHis: 1.1 ± 0.025	1.664ThrIle: 1.664 ± 0.037	1.263ThrLys: 1.263 ± 0.035	6.652ThrLeu: 6.652 ± 0.078	1.01ThrMet: 1.01 ± 0.026	1.288ThrAsn: 1.288 ± 0.038	3.652ThrPro: 3.652 ± 0.056	2.153ThrGln: 2.153 ± 0.041	3.367ThrArg: 3.367 ± 0.051	2.792ThrSer: 2.792 ± 0.053	3.024ThrThr: 3.024 ± 0.063	4.281ThrVal: 4.281 ± 0.067	0.758ThrTrp: 0.758 ± 0.025	1.121ThrTyr: 1.121 ± 0.038	0.003ThrXaa: 0.003 ± 0.001
Val	9.387ValAla: 9.387 ± 0.084	0.698ValCys: 0.698 ± 0.025	4.174ValAsp: 4.174 ± 0.064	3.857ValGlu: 3.857 ± 0.055	2.648ValPhe: 2.648 ± 0.051	5.495ValGly: 5.495 ± 0.069	1.538ValHis: 1.538 ± 0.035	3.131ValIle: 3.131 ± 0.048	2.396ValLys: 2.396 ± 0.05	8.401ValLeu: 8.401 ± 0.084	1.825ValMet: 1.825 ± 0.038	2.146ValAsn: 2.146 ± 0.044	3.647ValPro: 3.647 ± 0.045	3.126ValGln: 3.126 ± 0.044	4.732ValArg: 4.732 ± 0.06	4.225ValSer: 4.225 ± 0.057	4.009ValThr: 4.009 ± 0.074	5.895ValVal: 5.895 ± 0.08	1.029ValTrp: 1.029 ± 0.028	1.567ValTyr: 1.567 ± 0.039	0.004ValXaa: 0.004 ± 0.002
Trp	1.888TrpAla: 1.888 ± 0.039	0.194TrpCys: 0.194 ± 0.011	0.692TrpAsp: 0.692 ± 0.024	0.538TrpGlu: 0.538 ± 0.019	0.542TrpPhe: 0.542 ± 0.02	1.114TrpGly: 1.114 ± 0.033	0.349TrpHis: 0.349 ± 0.015	0.5TrpIle: 0.5 ± 0.018	0.449TrpLys: 0.449 ± 0.019	2.267TrpLeu: 2.267 ± 0.047	0.449TrpMet: 0.449 ± 0.022	0.461TrpAsn: 0.461 ± 0.019	0.84TrpPro: 0.84 ± 0.023	0.914TrpGln: 0.914 ± 0.028	1.472TrpArg: 1.472 ± 0.032	0.864TrpSer: 0.864 ± 0.029	0.829TrpThr: 0.829 ± 0.027	1.199TrpVal: 1.199 ± 0.033	0.328TrpTrp: 0.328 ± 0.017	0.297TrpTyr: 0.297 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.697TyrAla: 2.697 ± 0.045	0.23TyrCys: 0.23 ± 0.012	1.205TyrAsp: 1.205 ± 0.05	1.105TyrGlu: 1.105 ± 0.03	0.85TyrPhe: 0.85 ± 0.026	1.856TyrGly: 1.856 ± 0.04	0.407TyrHis: 0.407 ± 0.016	0.724TyrIle: 0.724 ± 0.021	0.63TyrLys: 0.63 ± 0.023	2.303TyrLeu: 2.303 ± 0.039	0.381TyrMet: 0.381 ± 0.017	0.666TyrAsn: 0.666 ± 0.024	1.016TyrPro: 1.016 ± 0.029	0.842TyrGln: 0.842 ± 0.029	1.711TyrArg: 1.711 ± 0.038	1.194TyrSer: 1.194 ± 0.033	1.276TyrThr: 1.276 ± 0.037	1.608TyrVal: 1.608 ± 0.037	0.394TyrTrp: 0.394 ± 0.018	0.584TyrTyr: 0.584 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.006XaaAla: 0.006 ± 0.002	0.002XaaCys: 0.002 ± 0.001	0.002XaaAsp: 0.002 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.005XaaGly: 0.005 ± 0.002	0.002XaaHis: 0.002 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.004XaaLeu: 0.004 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.004XaaVal: 0.004 ± 0.002	0.002XaaTrp: 0.002 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.087XaaXaa: 0.087 ± 0.01

Statistics based on 5253 proteins (1363903 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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