Murine coronavirus
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7B990|I7B990_9BETC Nucleoprotein OS=Murine coronavirus OX=694005 GN=N PE=3 SV=1
MM1 pKa = 7.47 LFVFILLLPSCLGYY15 pKa = 10.36 IGDD18 pKa = 4.3 FRR20 pKa = 11.84 CIQTVNYY27 pKa = 9.86 NGNNASAPSISTEE40 pKa = 3.73 AVDD43 pKa = 3.61 VSKK46 pKa = 11.15 GRR48 pKa = 11.84 GTYY51 pKa = 9.22 YY52 pKa = 11.01 VLDD55 pKa = 3.67 RR56 pKa = 11.84 VYY58 pKa = 11.47 LNATLLLTGYY68 pKa = 10.58 YY69 pKa = 9.74 PVDD72 pKa = 3.26 GSNYY76 pKa = 9.77 RR77 pKa = 11.84 NLALTGTNTLSLTWFKK93 pKa = 11.13 PPFLSEE99 pKa = 4.04 FNDD102 pKa = 5.06 GIFAKK107 pKa = 10.4 VQNLKK112 pKa = 9.78 TNTPTGATSYY122 pKa = 10.66 FPTIVIGSLFGNTSYY137 pKa = 10.68 TVVLEE142 pKa = 4.17 PYY144 pKa = 10.14 NNIIMASVCTYY155 pKa = 7.55 TICQLPYY162 pKa = 10.03 TPCKK166 pKa = 10.23 PNTNGNRR173 pKa = 11.84 VIGFWHH179 pKa = 7.05 TDD181 pKa = 3.24 VKK183 pKa = 11.12 PPICLLKK190 pKa = 10.88 RR191 pKa = 11.84 NFTFNVNAPWLYY203 pKa = 10.39 FHH205 pKa = 7.79 FYY207 pKa = 8.91 QQGGTFYY214 pKa = 10.77 AYY216 pKa = 10.83 YY217 pKa = 10.42 ADD219 pKa = 4.72 KK220 pKa = 10.83 PSATTFLFSVYY231 pKa = 10.07 IGDD234 pKa = 3.89 ILTQYY239 pKa = 10.48 FVLPFICTPTAGSTLAPLYY258 pKa = 9.98 WVTPLLKK265 pKa = 10.15 RR266 pKa = 11.84 QYY268 pKa = 10.93 LFNFNEE274 pKa = 4.21 KK275 pKa = 10.49 GVITSAVDD283 pKa = 3.33 CASSYY288 pKa = 10.66 ISEE291 pKa = 4.54 IKK293 pKa = 10.69 CKK295 pKa = 8.53 TQSLLPSTGVYY306 pKa = 10.24 DD307 pKa = 3.93 LSGYY311 pKa = 6.78 TVQPVGVVYY320 pKa = 10.46 RR321 pKa = 11.84 RR322 pKa = 11.84 VPNLPDD328 pKa = 3.5 CKK330 pKa = 9.93 IEE332 pKa = 3.81 EE333 pKa = 4.32 WLTAKK338 pKa = 10.27 SVPSPLNWEE347 pKa = 3.58 RR348 pKa = 11.84 RR349 pKa = 11.84 TFQNCNFNLSSLLRR363 pKa = 11.84 YY364 pKa = 8.69 VQAEE368 pKa = 4.13 SLSCNNIDD376 pKa = 3.31 ASKK379 pKa = 10.66 VYY381 pKa = 9.95 GMCFGSVSVDD391 pKa = 2.67 KK392 pKa = 10.62 FAIPRR397 pKa = 11.84 SRR399 pKa = 11.84 QIDD402 pKa = 3.88 LQIGNSGFLQTANYY416 pKa = 9.74 KK417 pKa = 9.91 IDD419 pKa = 3.64 TAATSCQLYY428 pKa = 10.65 YY429 pKa = 10.87 SLPKK433 pKa = 10.92 NNVTINNYY441 pKa = 9.54 NPSSWNRR448 pKa = 11.84 RR449 pKa = 11.84 YY450 pKa = 10.28 GFKK453 pKa = 11.13 VNDD456 pKa = 3.59 RR457 pKa = 11.84 CQIFANILLNGINSGTTCSTDD478 pKa = 3.14 LQLPNTEE485 pKa = 4.21 VATGVCVRR493 pKa = 11.84 YY494 pKa = 9.74 DD495 pKa = 3.44 LYY497 pKa = 11.29 GITGQGVFKK506 pKa = 10.25 EE507 pKa = 4.64 VKK509 pKa = 9.62 ADD511 pKa = 4.25 YY512 pKa = 10.05 YY513 pKa = 11.52 NSWQALLYY521 pKa = 10.55 DD522 pKa = 4.03 VNGNLNGFRR531 pKa = 11.84 DD532 pKa = 3.65 LTTNKK537 pKa = 9.29 TYY539 pKa = 10.09 TIRR542 pKa = 11.84 SCYY545 pKa = 9.26 SGRR548 pKa = 11.84 VSAAYY553 pKa = 9.67 HH554 pKa = 6.17 KK555 pKa = 8.08 EE556 pKa = 3.96 APEE559 pKa = 3.78 PALLYY564 pKa = 10.9 RR565 pKa = 11.84 NINCSYY571 pKa = 10.95 VFTNNISRR579 pKa = 11.84 EE580 pKa = 4.24 EE581 pKa = 3.99 NPLNYY586 pKa = 9.38 FDD588 pKa = 5.71 SYY590 pKa = 10.97 LGCVVNADD598 pKa = 3.38 NRR600 pKa = 11.84 TDD602 pKa = 3.5 EE603 pKa = 5.07 ALPNCDD609 pKa = 3.43 LRR611 pKa = 11.84 MGAGLCVDD619 pKa = 3.67 YY620 pKa = 11.11 SKK622 pKa = 11.14 SRR624 pKa = 11.84 RR625 pKa = 11.84 ARR627 pKa = 11.84 RR628 pKa = 11.84 SVSTGYY634 pKa = 11.01 RR635 pKa = 11.84 LTTFEE640 pKa = 5.37 PYY642 pKa = 9.99 MPMLVNDD649 pKa = 4.28 SVQSVGGLYY658 pKa = 10.45 EE659 pKa = 3.97 MQIPTNFTIGHH670 pKa = 6.13 HH671 pKa = 5.77 EE672 pKa = 4.09 EE673 pKa = 4.92 FIQIRR678 pKa = 11.84 APKK681 pKa = 8.75 VTIDD685 pKa = 3.67 CAAFVCGDD693 pKa = 3.4 NAACRR698 pKa = 11.84 QQLVEE703 pKa = 4.55 YY704 pKa = 10.87 GSFCDD709 pKa = 4.0 NVNAILNEE717 pKa = 3.97 VNNLLDD723 pKa = 4.08 NMQLQVASALMQGVTISSRR742 pKa = 11.84 LPDD745 pKa = 5.17 GISGPIDD752 pKa = 4.86 DD753 pKa = 5.34 INFSPLLGCIGSTCAEE769 pKa = 4.69 DD770 pKa = 4.47 GNGPSAIRR778 pKa = 11.84 GRR780 pKa = 11.84 SAIEE784 pKa = 4.11 DD785 pKa = 3.76 LLFDD789 pKa = 4.06 KK790 pKa = 11.25 VKK792 pKa = 10.82 LSDD795 pKa = 3.34 VGFVEE800 pKa = 6.19 AYY802 pKa = 9.87 NNCTGGQEE810 pKa = 4.32 VRR812 pKa = 11.84 DD813 pKa = 3.97 LLCVQSFNGIKK824 pKa = 10.05 VLPPVLSEE832 pKa = 4.18 SQISGYY838 pKa = 7.58 TAGATAAAMFPPWTAAAGVPFSLNVQYY865 pKa = 10.59 RR866 pKa = 11.84 INGLGVTMNVLSEE879 pKa = 4.11 NQKK882 pKa = 10.06 MIASAFNNALGAIQEE897 pKa = 4.62 GFDD900 pKa = 3.73 ATNSALGKK908 pKa = 9.37 IQSVVNANAEE918 pKa = 4.13 ALNNLLNQLSNRR930 pKa = 11.84 FGAISASLQEE940 pKa = 4.0 ILTRR944 pKa = 11.84 LDD946 pKa = 3.15 AVEE949 pKa = 5.13 AKK951 pKa = 10.57 AQIDD955 pKa = 3.56 RR956 pKa = 11.84 LINGRR961 pKa = 11.84 LTALNAYY968 pKa = 9.21 ISKK971 pKa = 9.99 QLSDD975 pKa = 3.23 STLIKK980 pKa = 10.46 FSAAQAIEE988 pKa = 4.11 KK989 pKa = 10.61 VNEE992 pKa = 4.28 CVKK995 pKa = 10.93 SQTTRR1000 pKa = 11.84 INFCGNGNHH1009 pKa = 6.86 ILSLVQNAPYY1019 pKa = 9.9 GLCFIHH1025 pKa = 6.84 FSYY1028 pKa = 11.19 VPTSFKK1034 pKa = 9.67 TANVSPGLCISGDD1047 pKa = 3.15 RR1048 pKa = 11.84 GLAPKK1053 pKa = 10.3 AGYY1056 pKa = 8.93 FVQDD1060 pKa = 3.0 NGEE1063 pKa = 4.07 WKK1065 pKa = 8.91 FTGSNYY1071 pKa = 10.17 YY1072 pKa = 9.98 YY1073 pKa = 9.83 PEE1075 pKa = 5.68 PITDD1079 pKa = 3.59 KK1080 pKa = 10.85 NSVVMISCAVNYY1092 pKa = 7.8 TKK1094 pKa = 10.77 APEE1097 pKa = 4.02 VFLNNSIPNLPDD1109 pKa = 3.66 FKK1111 pKa = 11.37 EE1112 pKa = 4.4 EE1113 pKa = 3.64 LDD1115 pKa = 3.46 KK1116 pKa = 11.03 WFKK1119 pKa = 10.55 NQTSIAPDD1127 pKa = 3.69 LSLDD1131 pKa = 3.81 FEE1133 pKa = 4.58 KK1134 pKa = 11.4 LNVTFLDD1141 pKa = 3.44 LTYY1144 pKa = 11.03 EE1145 pKa = 4.04 MNRR1148 pKa = 11.84 IQDD1151 pKa = 4.14 AIKK1154 pKa = 10.34 KK1155 pKa = 10.05 LNEE1158 pKa = 3.82 SYY1160 pKa = 11.14 INLKK1164 pKa = 10.23 EE1165 pKa = 3.97 VGTYY1169 pKa = 9.32 EE1170 pKa = 5.21 MYY1172 pKa = 11.04 VKK1174 pKa = 9.96 WPWYY1178 pKa = 8.24 VWLLIGLAGVAVCVLLFFICCCTGCGSCCFRR1209 pKa = 11.84 KK1210 pKa = 10.28 CGSCCDD1216 pKa = 3.87 EE1217 pKa = 4.38 YY1218 pKa = 11.48 GGHH1221 pKa = 6.11 QDD1223 pKa = 4.52 SIVIHH1228 pKa = 6.54 NISAHH1233 pKa = 5.82 EE1234 pKa = 4.06 DD1235 pKa = 3.09
Molecular weight: 136.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.438
IPC2_protein 5.703
IPC_protein 5.703
Toseland 5.728
ProMoST 5.919
Dawson 5.906
Bjellqvist 5.97
Wikipedia 5.881
Rodwell 5.868
Grimsley 5.728
Solomon 5.906
Lehninger 5.893
Nozaki 6.148
DTASelect 6.313
Thurlkill 6.237
EMBOSS 6.211
Sillero 6.237
Patrickios 0.795
IPC_peptide 5.919
IPC2_peptide 6.198
IPC2.peptide.svr19 6.111
Protein with the highest isoelectric point:
>tr|I7AB47|I7AB47_9BETC Hemagglutinin-esterase OS=Murine coronavirus OX=694005 GN=HE PE=3 SV=1
MM1 pKa = 7.46 ALIGPKK7 pKa = 7.57 TTIAAVFIGPFLVACMLGIGLVYY30 pKa = 10.81 LLQLQVQIFHH40 pKa = 6.45 VKK42 pKa = 9.07 DD43 pKa = 3.89 TIRR46 pKa = 11.84 VTGKK50 pKa = 9.2 PATVSYY56 pKa = 7.5 TTSTPVTPVATTLDD70 pKa = 3.53 GTTYY74 pKa = 10.5 TLIRR78 pKa = 11.84 PTSSYY83 pKa = 9.16 TRR85 pKa = 11.84 VYY87 pKa = 10.18 LGSSRR92 pKa = 11.84 GFDD95 pKa = 2.9 TSTFGPKK102 pKa = 9.08 TLDD105 pKa = 3.78 YY106 pKa = 9.39 ITSSKK111 pKa = 10.15 PHH113 pKa = 6.34 LNSGRR118 pKa = 11.84 PYY120 pKa = 10.28 TLRR123 pKa = 11.84 HH124 pKa = 5.44 LPKK127 pKa = 10.79 YY128 pKa = 6.04 MTPPATWRR136 pKa = 11.84 FGLL139 pKa = 4.06
Molecular weight: 15.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.09
IPC2_protein 9.838
IPC_protein 10.16
Toseland 9.955
ProMoST 9.823
Dawson 10.262
Bjellqvist 9.999
Wikipedia 10.482
Rodwell 10.496
Grimsley 10.365
Solomon 10.292
Lehninger 10.233
Nozaki 9.97
DTASelect 9.999
Thurlkill 10.072
EMBOSS 10.394
Sillero 10.16
Patrickios 7.483
IPC_peptide 10.277
IPC2_peptide 8.887
IPC2.peptide.svr19 8.628
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
14344
88
7180
1593.8
177.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.965 ± 0.17
3.758 ± 0.249
5.556 ± 0.333
4.037 ± 0.23
5.243 ± 0.109
6.177 ± 0.274
1.645 ± 0.158
4.19 ± 0.415
5.779 ± 0.38
9.126 ± 0.242
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.363 ± 0.192
4.971 ± 0.596
3.841 ± 0.293
3.437 ± 0.297
3.681 ± 0.251
7.139 ± 0.15
5.752 ± 0.345
10.213 ± 0.815
1.339 ± 0.108
4.769 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here