Torque teno virus
Average proteome isoelectric point is 7.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8V7H8|Q8V7H8_9VIRU Capsid protein OS=Torque teno virus OX=68887 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.39 PWSLPRR7 pKa = 11.84 HH8 pKa = 5.86 NIRR11 pKa = 11.84 TRR13 pKa = 11.84 EE14 pKa = 4.01 DD15 pKa = 3.01 LWVQSILYY23 pKa = 10.14 SHH25 pKa = 7.23 DD26 pKa = 3.69 TFCGCDD32 pKa = 3.75 NIPEE36 pKa = 4.6 HH37 pKa = 6.14 LTGLLGGVRR46 pKa = 11.84 PAPPRR51 pKa = 11.84 NPGPPTIRR59 pKa = 11.84 SLPALPPAPEE69 pKa = 4.26 PPEE72 pKa = 3.88 EE73 pKa = 4.04 PRR75 pKa = 11.84 RR76 pKa = 11.84 GGDD79 pKa = 3.05 TDD81 pKa = 3.6 GDD83 pKa = 3.75 RR84 pKa = 11.84 GEE86 pKa = 5.28 DD87 pKa = 3.44 GGDD90 pKa = 2.93 AAGAYY95 pKa = 9.42 EE96 pKa = 4.39 PEE98 pKa = 4.29 DD99 pKa = 4.0 LEE101 pKa = 4.46 EE102 pKa = 4.44 LFAAAEE108 pKa = 3.99 QDD110 pKa = 4.22 DD111 pKa = 4.0 MM112 pKa = 6.93
Molecular weight: 12.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.389
IPC2_protein 4.304
IPC_protein 4.228
Toseland 4.05
ProMoST 4.355
Dawson 4.19
Bjellqvist 4.342
Wikipedia 4.088
Rodwell 4.062
Grimsley 3.961
Solomon 4.177
Lehninger 4.139
Nozaki 4.304
DTASelect 4.482
Thurlkill 4.075
EMBOSS 4.101
Sillero 4.342
Patrickios 3.745
IPC_peptide 4.19
IPC2_peptide 4.329
IPC2.peptide.svr19 4.265
Protein with the highest isoelectric point:
>tr|Q8V7I0|Q8V7I0_9VIRU ORF4 OS=Torque teno virus OX=68887 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.39 PWSLPRR7 pKa = 11.84 HH8 pKa = 5.86 NIRR11 pKa = 11.84 TRR13 pKa = 11.84 EE14 pKa = 4.01 DD15 pKa = 3.01 LWVQSILYY23 pKa = 10.14 SHH25 pKa = 7.23 DD26 pKa = 3.69 TFCGCDD32 pKa = 3.75 NIPEE36 pKa = 4.6 HH37 pKa = 6.14 LTGLLGGVRR46 pKa = 11.84 PAPPRR51 pKa = 11.84 NPGPPTIRR59 pKa = 11.84 SLPALPPAPEE69 pKa = 4.26 PPEE72 pKa = 3.88 EE73 pKa = 4.04 PRR75 pKa = 11.84 RR76 pKa = 11.84 GGDD79 pKa = 3.05 TDD81 pKa = 3.6 GDD83 pKa = 3.75 RR84 pKa = 11.84 GEE86 pKa = 5.28 DD87 pKa = 3.44 GGDD90 pKa = 2.93 AAGAYY95 pKa = 9.42 EE96 pKa = 4.39 PEE98 pKa = 4.29 DD99 pKa = 4.0 LEE101 pKa = 4.46 EE102 pKa = 4.44 LFAAAEE108 pKa = 4.02 QDD110 pKa = 3.82 DD111 pKa = 3.88 MRR113 pKa = 11.84 RR114 pKa = 11.84 VQKK117 pKa = 9.78 TPIRR121 pKa = 11.84 HH122 pKa = 5.33 SPPRR126 pKa = 11.84 AAIKK130 pKa = 9.45 PRR132 pKa = 11.84 RR133 pKa = 11.84 RR134 pKa = 11.84 RR135 pKa = 11.84 LVYY138 pKa = 9.86 PQTTPKK144 pKa = 10.2 RR145 pKa = 11.84 ARR147 pKa = 11.84 RR148 pKa = 11.84 DD149 pKa = 3.42 HH150 pKa = 6.42 LRR152 pKa = 11.84 GRR154 pKa = 11.84 TSTPKK159 pKa = 10.15 RR160 pKa = 11.84 RR161 pKa = 11.84 GAKK164 pKa = 9.46 RR165 pKa = 11.84 KK166 pKa = 10.12 APTATQSPATAPASPQTGNQTPHH189 pKa = 5.98 GRR191 pKa = 11.84 RR192 pKa = 11.84 PPTQTGSPLEE202 pKa = 4.17 PSPIILPPEE211 pKa = 5.15 PIPDD215 pKa = 3.81 LLFPNTGKK223 pKa = 10.49 KK224 pKa = 10.06 KK225 pKa = 10.43 KK226 pKa = 10.23 FSLFDD231 pKa = 3.52 WEE233 pKa = 4.59 CEE235 pKa = 3.78 RR236 pKa = 11.84 DD237 pKa = 3.69 LACAFCRR244 pKa = 11.84 PMRR247 pKa = 11.84 FYY249 pKa = 10.71 PSDD252 pKa = 3.43 NPTYY256 pKa = 10.05 PWLPPKK262 pKa = 10.24 RR263 pKa = 11.84 DD264 pKa = 3.07 IPKK267 pKa = 8.76 ICKK270 pKa = 9.96 VNFKK274 pKa = 10.86 INFTEE279 pKa = 3.93
Molecular weight: 31.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.087
IPC2_protein 8.873
IPC_protein 8.902
Toseland 9.706
ProMoST 9.414
Dawson 9.926
Bjellqvist 9.663
Wikipedia 10.072
Rodwell 10.204
Grimsley 9.97
Solomon 9.97
Lehninger 9.94
Nozaki 9.882
DTASelect 9.589
Thurlkill 9.794
EMBOSS 10.131
Sillero 9.882
Patrickios 9.545
IPC_peptide 9.97
IPC2_peptide 8.521
IPC2.peptide.svr19 7.721
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1426
112
746
356.5
41.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.839 ± 0.878
1.753 ± 0.112
5.68 ± 1.029
5.54 ± 0.965
2.595 ± 0.439
5.68 ± 0.867
2.665 ± 0.491
4.628 ± 0.548
5.68 ± 0.856
7.223 ± 0.312
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.964 ± 0.478
4.278 ± 0.497
9.958 ± 2.487
4.208 ± 0.741
9.537 ± 0.725
6.522 ± 1.561
7.714 ± 0.732
2.735 ± 0.702
2.665 ± 0.733
4.137 ± 1.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here