Hubei sobemo-like virus 41

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE25|A0A1L3KE25_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 41 OX=1923229 PE=4 SV=1
MM1 pKa = 7.55YY2 pKa = 10.39IPVNKK7 pKa = 9.66VLYY10 pKa = 10.54VDD12 pKa = 4.37VIEE15 pKa = 5.11LKK17 pKa = 10.48IRR19 pKa = 11.84DD20 pKa = 3.27TWAAIEE26 pKa = 4.57SYY28 pKa = 11.44LMDD31 pKa = 3.5TPYY34 pKa = 10.84CRR36 pKa = 11.84TVEE39 pKa = 3.99SGFWFWKK46 pKa = 7.73EE47 pKa = 3.75THH49 pKa = 6.72CEE51 pKa = 4.06LRR53 pKa = 11.84WVPLMVTILLALVSYY68 pKa = 10.15FGLKK72 pKa = 10.15RR73 pKa = 11.84IVRR76 pKa = 11.84GCLKK80 pKa = 9.53PRR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84VTYY87 pKa = 9.89EE88 pKa = 3.46VDD90 pKa = 3.25PLLTQQEE97 pKa = 4.62SLVIGKK103 pKa = 9.06PLMPGGKK110 pKa = 8.71QPKK113 pKa = 9.23CQVTLATRR121 pKa = 11.84RR122 pKa = 11.84NDD124 pKa = 2.88VRR126 pKa = 11.84LLMGGGFRR134 pKa = 11.84LLDD137 pKa = 3.65YY138 pKa = 10.85LVLPTHH144 pKa = 6.72VMHH147 pKa = 7.33AGQGKK152 pKa = 9.92VYY154 pKa = 10.87VLANDD159 pKa = 4.15KK160 pKa = 10.52EE161 pKa = 4.32LEE163 pKa = 4.11IPKK166 pKa = 10.22DD167 pKa = 3.41AEE169 pKa = 4.53TIDD172 pKa = 4.05LAADD176 pKa = 4.18LLAVKK181 pKa = 10.29ISQAEE186 pKa = 3.86WSKK189 pKa = 11.11IGVSQAKK196 pKa = 9.29LGPMSGTPTVQVTSPCDD213 pKa = 3.19QKK215 pKa = 11.43FSIGYY220 pKa = 9.39LKK222 pKa = 10.4LAKK225 pKa = 10.37SFGRR229 pKa = 11.84VEE231 pKa = 4.44YY232 pKa = 10.53DD233 pKa = 3.11SSTQPGFSGAAYY245 pKa = 9.87MNGNVCLGMHH255 pKa = 6.31LHH257 pKa = 6.51GGVVAGGYY265 pKa = 9.22EE266 pKa = 3.89GLYY269 pKa = 9.68IYY271 pKa = 11.01ARR273 pKa = 11.84LKK275 pKa = 9.55HH276 pKa = 5.94HH277 pKa = 7.26LYY279 pKa = 9.88MNGVEE284 pKa = 5.0DD285 pKa = 4.35EE286 pKa = 4.4ASKK289 pKa = 9.93TSPGGSDD296 pKa = 3.19WAPDD300 pKa = 3.32YY301 pKa = 11.43HH302 pKa = 7.48DD303 pKa = 3.64INEE306 pKa = 4.13FEE308 pKa = 4.39EE309 pKa = 4.79LEE311 pKa = 4.08QKK313 pKa = 10.35IVDD316 pKa = 3.94GKK318 pKa = 8.78SEE320 pKa = 3.6RR321 pKa = 11.84RR322 pKa = 11.84AIVRR326 pKa = 11.84LKK328 pKa = 10.19SGHH331 pKa = 4.62YY332 pKa = 9.92HH333 pKa = 5.22ITRR336 pKa = 11.84GEE338 pKa = 4.06LLDD341 pKa = 4.24KK342 pKa = 10.74INRR345 pKa = 11.84LRR347 pKa = 11.84DD348 pKa = 3.41SNAWADD354 pKa = 3.46QMEE357 pKa = 4.66AEE359 pKa = 4.73DD360 pKa = 5.35AQAQLDD366 pKa = 3.88NKK368 pKa = 10.52EE369 pKa = 4.63YY370 pKa = 10.19IPEE373 pKa = 4.17AAIHH377 pKa = 6.08PGKK380 pKa = 10.48GVEE383 pKa = 4.64FSGEE387 pKa = 4.12GQRR390 pKa = 11.84PAGRR394 pKa = 11.84AAPGQEE400 pKa = 3.85QSRR403 pKa = 11.84SHH405 pKa = 6.79SDD407 pKa = 3.02SKK409 pKa = 11.4SSNGEE414 pKa = 3.27PRR416 pKa = 11.84RR417 pKa = 11.84PRR419 pKa = 11.84TEE421 pKa = 3.61RR422 pKa = 11.84DD423 pKa = 3.16RR424 pKa = 11.84LLLEE428 pKa = 4.53LSSVSNLQLRR438 pKa = 11.84EE439 pKa = 3.84YY440 pKa = 9.25LTSVKK445 pKa = 10.3NGRR448 pKa = 11.84RR449 pKa = 11.84QARR452 pKa = 11.84AMSLQIPPQEE462 pKa = 4.16RR463 pKa = 11.84NGNRR467 pKa = 11.84SALNSTEE474 pKa = 3.82SRR476 pKa = 11.84VV477 pKa = 3.31

Molecular weight: 53.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE25|A0A1L3KE25_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 41 OX=1923229 PE=4 SV=1
MM1 pKa = 7.43HH2 pKa = 7.23SGYY5 pKa = 10.67VIPADD10 pKa = 3.51WNSKK14 pKa = 8.77SRR16 pKa = 11.84FMTLLLTLDD25 pKa = 4.09YY26 pKa = 10.39SSSPGYY32 pKa = 9.69PYY34 pKa = 10.73LRR36 pKa = 11.84EE37 pKa = 4.07APTIGKK43 pKa = 8.3WLRR46 pKa = 11.84TDD48 pKa = 3.06EE49 pKa = 4.99CGSFDD54 pKa = 3.7PVQVEE59 pKa = 4.13RR60 pKa = 11.84LWYY63 pKa = 9.39DD64 pKa = 3.08VNMVMAGSYY73 pKa = 8.52EE74 pKa = 3.96HH75 pKa = 7.5LFRR78 pKa = 11.84AFVKK82 pKa = 10.68DD83 pKa = 4.06EE84 pKa = 3.7PHH86 pKa = 6.91KK87 pKa = 10.32IAKK90 pKa = 9.95AKK92 pKa = 8.56EE93 pKa = 3.88NRR95 pKa = 11.84WRR97 pKa = 11.84LIIASSLPVQMVWRR111 pKa = 11.84MLYY114 pKa = 9.65TGQNEE119 pKa = 4.52ALNKK123 pKa = 8.89YY124 pKa = 10.19HH125 pKa = 7.33DD126 pKa = 4.13WCPSKK131 pKa = 10.78HH132 pKa = 5.94GFVFCYY138 pKa = 9.65GGWMKK143 pKa = 10.43FIAQAKK149 pKa = 7.73TKK151 pKa = 9.54GLNVSRR157 pKa = 11.84DD158 pKa = 3.04ISGWDD163 pKa = 3.06VGAPGWVFEE172 pKa = 4.66VVGAWRR178 pKa = 11.84EE179 pKa = 3.94SWPRR183 pKa = 11.84ATDD186 pKa = 2.67SWIRR190 pKa = 11.84VHH192 pKa = 8.75RR193 pKa = 11.84MMYY196 pKa = 10.67DD197 pKa = 3.29DD198 pKa = 5.04AYY200 pKa = 9.67KK201 pKa = 10.16SSRR204 pKa = 11.84IIFSNGVVVRR214 pKa = 11.84QLFGGFMKK222 pKa = 10.58SGLFNTISDD231 pKa = 3.57NSLAMGAIHH240 pKa = 6.95SLACVRR246 pKa = 11.84SGLPFGHH253 pKa = 6.63YY254 pKa = 9.78IVTGDD259 pKa = 5.56DD260 pKa = 3.21IAQSTVSAGYY270 pKa = 10.76LDD272 pKa = 4.71ALYY275 pKa = 11.13KK276 pKa = 10.47LGCRR280 pKa = 11.84VKK282 pKa = 10.17EE283 pKa = 3.97VLYY286 pKa = 10.22HH287 pKa = 6.0LEE289 pKa = 3.86FMGVNFSSGKK299 pKa = 8.65PEE301 pKa = 3.65PMYY304 pKa = 9.36TQKK307 pKa = 11.01HH308 pKa = 5.49LFNLLTKK315 pKa = 9.53EE316 pKa = 3.92QFIAEE321 pKa = 4.38TMDD324 pKa = 3.28SYY326 pKa = 11.47CRR328 pKa = 11.84YY329 pKa = 8.72YY330 pKa = 11.12AEE332 pKa = 3.96SSKK335 pKa = 9.47YY336 pKa = 10.03HH337 pKa = 6.61WFTEE341 pKa = 4.14IAKK344 pKa = 9.8EE345 pKa = 3.56LAVPVKK351 pKa = 10.68SRR353 pKa = 11.84WFYY356 pKa = 9.4QFWYY360 pKa = 9.91SSPLAEE366 pKa = 5.03IYY368 pKa = 10.47HH369 pKa = 6.25KK370 pKa = 10.98LGG372 pKa = 3.41

Molecular weight: 42.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

849

372

477

424.5

48.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.832 ± 0.077

1.413 ± 0.138

4.829 ± 0.18

6.125 ± 0.891

3.416 ± 1.358

7.892 ± 0.439

2.827 ± 0.276

4.476 ± 0.065

5.771 ± 0.099

9.187 ± 0.964

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.062 ± 0.486

3.18 ± 0.155

4.594 ± 0.389

3.534 ± 0.772

6.36 ± 0.868

8.009 ± 0.597

4.005 ± 0.167

6.949 ± 0.028

2.591 ± 0.998

4.947 ± 0.856
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski