Simian immunodeficiency virus - agm.tan-1
Average proteome isoelectric point is 7.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|P89907|P89907_SIVTA Protein Tat OS=Simian immunodeficiency virus - agm.tan-1 OX=349975 GN=tat PE=3 SV=1
MM1 pKa = 7.99 AEE3 pKa = 4.05 GRR5 pKa = 11.84 DD6 pKa = 3.6 SRR8 pKa = 11.84 EE9 pKa = 3.75 RR10 pKa = 11.84 RR11 pKa = 11.84 PGWLEE16 pKa = 3.13 IWDD19 pKa = 4.36 LSRR22 pKa = 11.84 EE23 pKa = 4.18 PWDD26 pKa = 3.46 EE27 pKa = 3.77
Molecular weight: 3.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.844
IPC2_protein 4.736
IPC_protein 4.457
Toseland 4.317
ProMoST 4.418
Dawson 4.393
Bjellqvist 4.673
Wikipedia 4.279
Rodwell 4.304
Grimsley 4.228
Solomon 4.38
Lehninger 4.342
Nozaki 4.52
DTASelect 4.635
Thurlkill 4.329
EMBOSS 4.291
Sillero 4.558
Patrickios 4.495
IPC_peptide 4.393
IPC2_peptide 4.558
IPC2.peptide.svr19 4.528
Protein with the highest isoelectric point:
>tr|P89909|P89909_SIVTA Envelope glycoprotein gp160 OS=Simian immunodeficiency virus - agm.tan-1 OX=349975 GN=env PE=4 SV=2
MM1 pKa = 7.42 SLGSEE6 pKa = 4.06 DD7 pKa = 3.92 LRR9 pKa = 11.84 RR10 pKa = 11.84 IIQIIRR16 pKa = 11.84 ILYY19 pKa = 9.81 HH20 pKa = 6.23 SNQYY24 pKa = 10.23 PPGEE28 pKa = 3.96 GTARR32 pKa = 11.84 QRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 QRR40 pKa = 11.84 FQQQQRR46 pKa = 11.84 QVAALSEE53 pKa = 4.57 RR54 pKa = 11.84 IFIAQQRR61 pKa = 11.84 RR62 pKa = 11.84 DD63 pKa = 3.63 PSGGEE68 pKa = 3.84 SLAAAFDD75 pKa = 3.73 QLVLDD80 pKa = 4.07 NQQLVIEE87 pKa = 4.64 TLPDD91 pKa = 4.06 PPQEE95 pKa = 4.13 PHH97 pKa = 7.16 DD98 pKa = 4.58 SSSTAA103 pKa = 3.19
Molecular weight: 11.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.14
IPC2_protein 9.107
IPC_protein 10.409
Toseland 9.648
ProMoST 9.823
Dawson 9.999
Bjellqvist 9.911
Wikipedia 10.365
Rodwell 9.823
Grimsley 10.145
Solomon 10.204
Lehninger 10.16
Nozaki 9.56
DTASelect 9.911
Thurlkill 9.78
EMBOSS 10.087
Sillero 9.911
Patrickios 7.673
IPC_peptide 10.189
IPC2_peptide 9.151
IPC2.peptide.svr19 8.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2922
27
1067
417.4
47.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.126 ± 0.368
2.43 ± 0.515
3.97 ± 0.41
6.776 ± 0.472
2.875 ± 0.153
7.495 ± 0.792
1.951 ± 0.248
5.852 ± 0.854
6.947 ± 0.78
8.385 ± 0.312
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.053 ± 0.163
4.62 ± 0.661
5.373 ± 0.647
6.468 ± 0.596
5.955 ± 0.647
5.236 ± 0.728
5.989 ± 0.884
5.578 ± 0.346
3.114 ± 0.495
2.806 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here