Rhizobium sp. 24NR

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4834 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A444LLJ9|A0A444LLJ9_9RHIZ ABC transporter permease OS=Rhizobium sp. 24NR OX=2503024 GN=EPK99_02170 PE=3 SV=1
MM1 pKa = 7.77AFQSVRR7 pKa = 11.84KK8 pKa = 8.23VSEE11 pKa = 3.77VARR14 pKa = 11.84YY15 pKa = 7.66VQYY18 pKa = 11.1AIAVAFLYY26 pKa = 10.9LLIPAFGVQAATSPYY41 pKa = 8.54CTNLSANVTQGSSVVIDD58 pKa = 3.61VSNCDD63 pKa = 3.3GPIGLGIGWNGAQPAHH79 pKa = 5.3GTIVIGNQTGPQGMQSVTYY98 pKa = 9.33TPNSNNTATSDD109 pKa = 3.66SFPLEE114 pKa = 4.45DD115 pKa = 3.61EE116 pKa = 4.52NGDD119 pKa = 3.7VFTVNITITPATSASIAVSPSSANEE144 pKa = 3.71DD145 pKa = 3.29SGATFTYY152 pKa = 10.03TVTLSQTSNSATTVNLTRR170 pKa = 11.84SGTATSGTDD179 pKa = 3.11YY180 pKa = 10.54TGAVSSIVVPANTTTASFVVTPVADD205 pKa = 3.61AAVEE209 pKa = 3.8ADD211 pKa = 3.67EE212 pKa = 4.49TVVISVASGSGYY224 pKa = 10.7SIGNPSSATATILNDD239 pKa = 3.88DD240 pKa = 4.56APSASIAVSPSSVAEE255 pKa = 4.38DD256 pKa = 3.62GATNLIYY263 pKa = 10.31TVTLSQAPVTAVSVAFNVGGTATSGSDD290 pKa = 3.46YY291 pKa = 11.15AAVSSPLVIAAGQTSGTITINPTADD316 pKa = 3.24SAVEE320 pKa = 3.7ADD322 pKa = 3.51EE323 pKa = 4.63TVILTLAAGSGYY335 pKa = 10.74SVGSPNSATGTILNDD350 pKa = 3.75DD351 pKa = 4.08QPSLTINDD359 pKa = 3.41VSITEE364 pKa = 4.39GNAGTSTATFTVSLNQPAGAGGVSFDD390 pKa = 3.7IATADD395 pKa = 3.52GTATAGVDD403 pKa = 3.77YY404 pKa = 10.64VASSLSRR411 pKa = 11.84TIAAGSSSATFTVLINGDD429 pKa = 3.84TLNEE433 pKa = 4.03PNEE436 pKa = 4.27TFFVNVSNVTGATVADD452 pKa = 3.91AQGQGTIVNDD462 pKa = 3.96DD463 pKa = 4.0SAPSLAIDD471 pKa = 3.5DD472 pKa = 4.15VSVNEE477 pKa = 4.47GNSGTTTATFTVSLSAASGQTVSVSYY503 pKa = 9.31ATVDD507 pKa = 3.53GTASAGSDD515 pKa = 3.26YY516 pKa = 10.87VARR519 pKa = 11.84SGTLTFAPGITAQGVAVTVNGDD541 pKa = 3.37TSVEE545 pKa = 4.05PNEE548 pKa = 4.28TFSVGLSGASNASIATGTGTGTGTGTGTGTINNDD582 pKa = 3.47DD583 pKa = 3.74AVVTVGPTTLPSATAASAYY602 pKa = 9.99NQTLTASGGTSPYY615 pKa = 10.93AFLVSAGALPSGLTLSSAGVLSGTPTASGSFNFTATATDD654 pKa = 3.43SGGSPTSGSRR664 pKa = 11.84AYY666 pKa = 8.72TLAVAGPTVDD676 pKa = 5.57LPATTLTGGVTGQAYY691 pKa = 9.42AASINPATGGIAPYY705 pKa = 9.83TYY707 pKa = 10.5TLGNGALPAGIVVDD721 pKa = 4.18SASGALSGTPTAAGTFNFTLTATDD745 pKa = 3.69STAGTPSQASRR756 pKa = 11.84SYY758 pKa = 10.99SLTVGQPSLSISPPTLDD775 pKa = 3.3AAVIGSPYY783 pKa = 10.74SKK785 pKa = 10.78VLVGSGGTAPYY796 pKa = 10.78SFVLGSGALPAGLTLAGNGTLSGTPTANGNFSFDD830 pKa = 3.34ATVTDD835 pKa = 4.61AYY837 pKa = 10.65GATASKK843 pKa = 10.8SYY845 pKa = 9.55TLAVNALPSLSIGDD859 pKa = 3.36ISVNEE864 pKa = 4.32GSSGTSIANLTVEE877 pKa = 4.96LSTASGQTVTVNYY890 pKa = 8.07ATADD894 pKa = 3.67GTASAGSDD902 pKa = 3.34YY903 pKa = 11.07VAASGALTFAPGVTTRR919 pKa = 11.84TIAVSINSDD928 pKa = 3.72TNPEE932 pKa = 3.79ASEE935 pKa = 4.32TFSVALSGAVNATIARR951 pKa = 11.84ATGTATIVDD960 pKa = 4.09DD961 pKa = 4.64DD962 pKa = 4.7APVTVGPASLSAATVNAPYY981 pKa = 10.2SQSLTATGGTAPYY994 pKa = 9.23TYY996 pKa = 10.93GVTTGSLPVGLTLSSAGALSGTPTTTGSFSFTASATDD1033 pKa = 3.19SSTPPSTASRR1043 pKa = 11.84SYY1045 pKa = 10.73SIAVSGPVMTLPATTLADD1063 pKa = 3.34AVTGQAYY1070 pKa = 9.95SAAITPATGGTAPYY1084 pKa = 9.12TYY1086 pKa = 10.45AVSAGTLPAGIILNSADD1103 pKa = 3.49GSLSGTPTASGTFTFTLTATDD1124 pKa = 3.82STGGTASQASRR1135 pKa = 11.84SYY1137 pKa = 10.58SLAVGTPSLSVSPPTLDD1154 pKa = 3.17AAVNGRR1160 pKa = 11.84AYY1162 pKa = 10.96SKK1164 pKa = 10.99VLAGSGGTAPYY1175 pKa = 10.12TFLLGSGSLPAGLTLTSNGALSGTPSVNGSFSFNVTVTDD1214 pKa = 3.27THH1216 pKa = 6.64GATGSQSYY1224 pKa = 8.03TLEE1227 pKa = 3.77VKK1229 pKa = 10.79APVGFVFSPSGGALPEE1245 pKa = 3.99AMIGEE1250 pKa = 4.97AYY1252 pKa = 9.85SANISATGGTGALIYY1267 pKa = 10.49SLASGTMPDD1276 pKa = 3.77GLVLNISTGQLNGPLSEE1293 pKa = 4.04AAKK1296 pKa = 10.04SGEE1299 pKa = 3.93YY1300 pKa = 10.27SFSIGVRR1307 pKa = 11.84DD1308 pKa = 3.85SNGNTGSASYY1318 pKa = 9.83VVKK1321 pKa = 10.8VIEE1324 pKa = 4.19RR1325 pKa = 11.84TVTVTNKK1332 pKa = 9.62VVDD1335 pKa = 4.34VPAGTVPPNVYY1346 pKa = 10.26LNRR1349 pKa = 11.84GATGGPFVDD1358 pKa = 3.61ADD1360 pKa = 4.07VIAVEE1365 pKa = 4.24PANAGSIQIIRR1376 pKa = 11.84GEE1378 pKa = 4.19VAQVGPVTPVGFYY1391 pKa = 10.97LKK1393 pKa = 10.3FIPNPAFSGQVNVGYY1408 pKa = 10.56RR1409 pKa = 11.84LISALGVSNQGLVTYY1424 pKa = 10.68NVGLNAAAVAEE1435 pKa = 5.06DD1436 pKa = 4.03IDD1438 pKa = 4.26TLVHH1442 pKa = 6.41GFVQTRR1448 pKa = 11.84QNLIASTINVPGLLEE1463 pKa = 3.84RR1464 pKa = 11.84RR1465 pKa = 11.84RR1466 pKa = 11.84MEE1468 pKa = 4.11EE1469 pKa = 3.37ATDD1472 pKa = 3.8PVTLRR1477 pKa = 11.84MNPDD1481 pKa = 3.43AEE1483 pKa = 4.26GMTASFSTSLVQLQGARR1500 pKa = 11.84DD1501 pKa = 3.57NADD1504 pKa = 3.39GLKK1507 pKa = 10.37SGASSPFNIWLEE1519 pKa = 3.98GTMIFHH1525 pKa = 6.99NRR1527 pKa = 11.84DD1528 pKa = 3.16EE1529 pKa = 5.26NGGKK1533 pKa = 8.83WGNFSMLSTGADD1545 pKa = 3.35YY1546 pKa = 11.34LLSDD1550 pKa = 4.05KK1551 pKa = 11.05ALVGISFHH1559 pKa = 6.81YY1560 pKa = 11.06DD1561 pKa = 2.56HH1562 pKa = 6.61MTDD1565 pKa = 3.29PTEE1568 pKa = 4.15EE1569 pKa = 4.1DD1570 pKa = 3.95AEE1572 pKa = 4.35LTGNGWLAGPYY1583 pKa = 10.44ASFEE1587 pKa = 3.81IGKK1590 pKa = 10.01GVFFDD1595 pKa = 3.57TSLLYY1600 pKa = 10.82GGSSNDD1606 pKa = 2.61IDD1608 pKa = 3.65TAFFDD1613 pKa = 4.13GTFDD1617 pKa = 3.34TSRR1620 pKa = 11.84WLFDD1624 pKa = 3.77SAIKK1628 pKa = 9.8GQWDD1632 pKa = 3.3IDD1634 pKa = 3.78DD1635 pKa = 4.21ATILTPKK1642 pKa = 9.54LRR1644 pKa = 11.84AMYY1647 pKa = 10.27INEE1650 pKa = 4.13KK1651 pKa = 10.05VDD1653 pKa = 4.35DD1654 pKa = 4.16YY1655 pKa = 11.79SVSNSGGDD1663 pKa = 3.33IVDD1666 pKa = 3.85LAGFTEE1672 pKa = 4.12EE1673 pKa = 3.96QLRR1676 pKa = 11.84VSLGAEE1682 pKa = 3.97IKK1684 pKa = 10.42RR1685 pKa = 11.84EE1686 pKa = 3.92YY1687 pKa = 10.53KK1688 pKa = 10.15LQNGASLTPSLGLTAGYY1705 pKa = 10.63AGLDD1709 pKa = 3.33GDD1711 pKa = 4.69GLFGSLAAGISLSTDD1726 pKa = 3.43TAWTFEE1732 pKa = 4.03AGVLFNIEE1740 pKa = 3.84GDD1742 pKa = 4.07GQQSTGAKK1750 pKa = 9.42ISARR1754 pKa = 11.84SQFF1757 pKa = 3.26

Molecular weight: 174.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S3RXQ3|A0A3S3RXQ3_9RHIZ FAD-binding oxidoreductase OS=Rhizobium sp. 24NR OX=2503024 GN=EPK99_01080 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27GGQKK29 pKa = 9.83VLSARR34 pKa = 11.84RR35 pKa = 11.84GRR37 pKa = 11.84GRR39 pKa = 11.84QRR41 pKa = 11.84LSAA44 pKa = 4.11

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4834

0

4834

1524728

26

2822

315.4

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.707 ± 0.043

0.746 ± 0.01

5.723 ± 0.027

5.727 ± 0.036

3.866 ± 0.022

8.318 ± 0.03

1.992 ± 0.018

5.71 ± 0.026

3.699 ± 0.025

10.056 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.598 ± 0.017

2.817 ± 0.017

4.93 ± 0.026

3.268 ± 0.02

6.599 ± 0.034

5.99 ± 0.03

5.339 ± 0.026

7.388 ± 0.026

1.247 ± 0.014

2.279 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski