Amino acid dipepetide frequency for Pelagibacter phage HTVC200P

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.945AlaAla: 5.945 ± 1.076
0.602AlaCys: 0.602 ± 0.166
4.29AlaAsp: 4.29 ± 0.542
4.29AlaGlu: 4.29 ± 0.579
2.559AlaPhe: 2.559 ± 0.407
6.472AlaGly: 6.472 ± 1.664
0.602AlaHis: 0.602 ± 0.248
3.612AlaIle: 3.612 ± 0.424
6.096AlaLys: 6.096 ± 0.825
5.795AlaLeu: 5.795 ± 0.637
1.731AlaMet: 1.731 ± 0.417
5.494AlaAsn: 5.494 ± 1.578
2.634AlaPro: 2.634 ± 0.553
3.161AlaGln: 3.161 ± 0.442
3.311AlaArg: 3.311 ± 0.552
5.193AlaSer: 5.193 ± 0.646
6.096AlaThr: 6.096 ± 1.25
5.117AlaVal: 5.117 ± 0.594
1.054AlaTrp: 1.054 ± 0.312
3.688AlaTyr: 3.688 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
0.677CysAla: 0.677 ± 0.22
0.0CysCys: 0.0 ± 0.0
0.376CysAsp: 0.376 ± 0.144
0.828CysGlu: 0.828 ± 0.251
0.376CysPhe: 0.376 ± 0.184
0.301CysGly: 0.301 ± 0.143
0.151CysHis: 0.151 ± 0.115
0.602CysIle: 0.602 ± 0.201
0.828CysLys: 0.828 ± 0.257
1.204CysLeu: 1.204 ± 0.326
0.075CysMet: 0.075 ± 0.071
0.602CysAsn: 0.602 ± 0.218
0.677CysPro: 0.677 ± 0.215
0.226CysGln: 0.226 ± 0.168
0.301CysArg: 0.301 ± 0.175
0.602CysSer: 0.602 ± 0.213
0.527CysThr: 0.527 ± 0.146
0.527CysVal: 0.527 ± 0.178
0.075CysTrp: 0.075 ± 0.067
0.075CysTyr: 0.075 ± 0.064
0.0CysXaa: 0.0 ± 0.0
Asp
5.117AspAla: 5.117 ± 0.68
0.753AspCys: 0.753 ± 0.255
3.161AspAsp: 3.161 ± 0.469
2.935AspGlu: 2.935 ± 0.551
2.784AspPhe: 2.784 ± 0.371
4.666AspGly: 4.666 ± 0.567
0.301AspHis: 0.301 ± 0.128
3.161AspIle: 3.161 ± 0.385
4.214AspLys: 4.214 ± 0.65
5.268AspLeu: 5.268 ± 0.746
1.656AspMet: 1.656 ± 0.322
2.483AspAsn: 2.483 ± 0.454
2.483AspPro: 2.483 ± 0.447
2.107AspGln: 2.107 ± 0.322
2.032AspArg: 2.032 ± 0.387
3.612AspSer: 3.612 ± 0.445
3.537AspThr: 3.537 ± 0.508
3.612AspVal: 3.612 ± 0.532
0.828AspTrp: 0.828 ± 0.234
2.408AspTyr: 2.408 ± 0.431
0.0AspXaa: 0.0 ± 0.0
Glu
4.591GluAla: 4.591 ± 0.529
0.602GluCys: 0.602 ± 0.253
3.462GluAsp: 3.462 ± 0.545
4.591GluGlu: 4.591 ± 0.699
2.182GluPhe: 2.182 ± 0.346
2.483GluGly: 2.483 ± 0.392
0.828GluHis: 0.828 ± 0.302
3.085GluIle: 3.085 ± 0.514
4.666GluLys: 4.666 ± 0.74
5.87GluLeu: 5.87 ± 0.962
1.43GluMet: 1.43 ± 0.384
2.709GluAsn: 2.709 ± 0.475
1.204GluPro: 1.204 ± 0.412
2.709GluGln: 2.709 ± 0.524
3.01GluArg: 3.01 ± 0.424
2.333GluSer: 2.333 ± 0.371
3.612GluThr: 3.612 ± 0.502
3.085GluVal: 3.085 ± 0.533
1.054GluTrp: 1.054 ± 0.276
2.333GluTyr: 2.333 ± 0.346
0.0GluXaa: 0.0 ± 0.0
Phe
2.559PheAla: 2.559 ± 0.353
0.376PheCys: 0.376 ± 0.165
2.483PheAsp: 2.483 ± 0.351
1.806PheGlu: 1.806 ± 0.425
1.957PhePhe: 1.957 ± 0.275
3.236PheGly: 3.236 ± 0.555
0.452PheHis: 0.452 ± 0.195
2.709PheIle: 2.709 ± 0.455
3.311PheLys: 3.311 ± 0.438
2.935PheLeu: 2.935 ± 0.429
0.828PheMet: 0.828 ± 0.25
2.935PheAsn: 2.935 ± 0.454
1.355PhePro: 1.355 ± 0.277
1.58PheGln: 1.58 ± 0.338
1.806PheArg: 1.806 ± 0.364
2.709PheSer: 2.709 ± 0.426
3.387PheThr: 3.387 ± 0.581
2.032PheVal: 2.032 ± 0.445
0.301PheTrp: 0.301 ± 0.132
1.054PheTyr: 1.054 ± 0.293
0.0PheXaa: 0.0 ± 0.0
Gly
4.365GlyAla: 4.365 ± 1.17
0.151GlyCys: 0.151 ± 0.108
3.989GlyAsp: 3.989 ± 0.609
3.236GlyGlu: 3.236 ± 0.453
4.214GlyPhe: 4.214 ± 0.612
9.708GlyGly: 9.708 ± 4.837
1.279GlyHis: 1.279 ± 0.424
4.365GlyIle: 4.365 ± 0.589
4.666GlyLys: 4.666 ± 0.595
4.816GlyLeu: 4.816 ± 0.729
0.978GlyMet: 0.978 ± 0.245
6.246GlyAsn: 6.246 ± 0.981
1.505GlyPro: 1.505 ± 0.322
2.483GlyGln: 2.483 ± 0.605
3.161GlyArg: 3.161 ± 0.564
7.526GlySer: 7.526 ± 1.541
6.848GlyThr: 6.848 ± 1.371
3.387GlyVal: 3.387 ± 0.579
0.828GlyTrp: 0.828 ± 0.277
2.935GlyTyr: 2.935 ± 0.465
0.0GlyXaa: 0.0 ± 0.0
His
0.903HisAla: 0.903 ± 0.27
0.301HisCys: 0.301 ± 0.184
0.527HisAsp: 0.527 ± 0.185
0.978HisGlu: 0.978 ± 0.338
0.753HisPhe: 0.753 ± 0.227
1.054HisGly: 1.054 ± 0.225
0.452HisHis: 0.452 ± 0.175
0.602HisIle: 0.602 ± 0.216
1.279HisLys: 1.279 ± 0.38
1.731HisLeu: 1.731 ± 0.535
0.452HisMet: 0.452 ± 0.173
0.677HisAsn: 0.677 ± 0.199
0.301HisPro: 0.301 ± 0.174
0.226HisGln: 0.226 ± 0.132
0.226HisArg: 0.226 ± 0.123
1.054HisSer: 1.054 ± 0.288
0.978HisThr: 0.978 ± 0.316
1.054HisVal: 1.054 ± 0.251
0.376HisTrp: 0.376 ± 0.149
0.677HisTyr: 0.677 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
5.569IleAla: 5.569 ± 0.652
0.452IleCys: 0.452 ± 0.204
4.365IleAsp: 4.365 ± 0.638
3.989IleGlu: 3.989 ± 0.61
1.656IlePhe: 1.656 ± 0.373
5.268IleGly: 5.268 ± 0.558
1.054IleHis: 1.054 ± 0.328
3.763IleIle: 3.763 ± 0.615
5.795IleLys: 5.795 ± 0.682
4.741IleLeu: 4.741 ± 0.774
0.903IleMet: 0.903 ± 0.289
4.741IleAsn: 4.741 ± 0.715
2.182IlePro: 2.182 ± 0.328
2.107IleGln: 2.107 ± 0.327
2.483IleArg: 2.483 ± 0.452
4.591IleSer: 4.591 ± 0.66
5.644IleThr: 5.644 ± 1.073
2.86IleVal: 2.86 ± 0.527
1.204IleTrp: 1.204 ± 0.286
2.333IleTyr: 2.333 ± 0.501
0.0IleXaa: 0.0 ± 0.0
Lys
5.945LysAla: 5.945 ± 0.967
0.226LysCys: 0.226 ± 0.112
4.515LysAsp: 4.515 ± 0.739
5.343LysGlu: 5.343 ± 0.79
3.085LysPhe: 3.085 ± 0.573
4.666LysGly: 4.666 ± 0.649
1.054LysHis: 1.054 ± 0.24
5.795LysIle: 5.795 ± 0.674
8.429LysLys: 8.429 ± 1.529
7.149LysLeu: 7.149 ± 1.017
2.107LysMet: 2.107 ± 0.49
4.515LysAsn: 4.515 ± 0.723
2.408LysPro: 2.408 ± 0.49
3.387LysGln: 3.387 ± 0.624
3.838LysArg: 3.838 ± 0.799
5.569LysSer: 5.569 ± 0.71
5.117LysThr: 5.117 ± 0.75
4.139LysVal: 4.139 ± 0.485
0.903LysTrp: 0.903 ± 0.363
3.236LysTyr: 3.236 ± 0.482
0.0LysXaa: 0.0 ± 0.0
Leu
6.472LeuAla: 6.472 ± 0.665
1.43LeuCys: 1.43 ± 0.437
5.87LeuAsp: 5.87 ± 0.606
4.892LeuGlu: 4.892 ± 0.672
2.107LeuPhe: 2.107 ± 0.413
3.989LeuGly: 3.989 ± 0.536
0.527LeuHis: 0.527 ± 0.212
4.44LeuIle: 4.44 ± 0.533
7.977LeuLys: 7.977 ± 1.094
5.418LeuLeu: 5.418 ± 0.976
1.731LeuMet: 1.731 ± 0.358
4.44LeuAsn: 4.44 ± 0.588
3.311LeuPro: 3.311 ± 0.516
3.311LeuGln: 3.311 ± 0.445
3.161LeuArg: 3.161 ± 0.514
5.644LeuSer: 5.644 ± 0.703
4.365LeuThr: 4.365 ± 0.487
4.139LeuVal: 4.139 ± 0.604
0.602LeuTrp: 0.602 ± 0.272
2.86LeuTyr: 2.86 ± 0.462
0.0LeuXaa: 0.0 ± 0.0
Met
2.182MetAla: 2.182 ± 0.344
0.376MetCys: 0.376 ± 0.188
0.602MetAsp: 0.602 ± 0.206
1.279MetGlu: 1.279 ± 0.306
0.527MetPhe: 0.527 ± 0.192
1.505MetGly: 1.505 ± 0.404
0.602MetHis: 0.602 ± 0.234
1.129MetIle: 1.129 ± 0.311
1.806MetLys: 1.806 ± 0.416
1.43MetLeu: 1.43 ± 0.445
0.226MetMet: 0.226 ± 0.115
1.129MetAsn: 1.129 ± 0.25
0.903MetPro: 0.903 ± 0.262
0.452MetGln: 0.452 ± 0.191
0.903MetArg: 0.903 ± 0.241
1.806MetSer: 1.806 ± 0.309
0.903MetThr: 0.903 ± 0.245
1.054MetVal: 1.054 ± 0.355
0.376MetTrp: 0.376 ± 0.204
0.677MetTyr: 0.677 ± 0.213
0.0MetXaa: 0.0 ± 0.0
Asn
4.591AsnAla: 4.591 ± 1.027
0.677AsnCys: 0.677 ± 0.233
3.085AsnAsp: 3.085 ± 0.462
3.311AsnGlu: 3.311 ± 0.43
3.085AsnPhe: 3.085 ± 0.483
6.397AsnGly: 6.397 ± 0.93
0.753AsnHis: 0.753 ± 0.267
6.02AsnIle: 6.02 ± 1.142
4.591AsnLys: 4.591 ± 0.785
3.913AsnLeu: 3.913 ± 0.488
1.129AsnMet: 1.129 ± 0.236
4.29AsnAsn: 4.29 ± 0.482
1.957AsnPro: 1.957 ± 0.459
1.355AsnGln: 1.355 ± 0.252
2.032AsnArg: 2.032 ± 0.4
4.666AsnSer: 4.666 ± 0.96
5.87AsnThr: 5.87 ± 0.789
4.816AsnVal: 4.816 ± 0.721
0.527AsnTrp: 0.527 ± 0.191
2.258AsnTyr: 2.258 ± 0.492
0.0AsnXaa: 0.0 ± 0.0
Pro
2.258ProAla: 2.258 ± 0.477
0.376ProCys: 0.376 ± 0.142
1.58ProAsp: 1.58 ± 0.217
1.881ProGlu: 1.881 ± 0.442
1.43ProPhe: 1.43 ± 0.272
1.43ProGly: 1.43 ± 0.393
0.527ProHis: 0.527 ± 0.151
1.129ProIle: 1.129 ± 0.327
3.01ProLys: 3.01 ± 0.534
1.806ProLeu: 1.806 ± 0.476
0.903ProMet: 0.903 ± 0.242
1.731ProAsn: 1.731 ± 0.306
0.978ProPro: 0.978 ± 0.282
0.677ProGln: 0.677 ± 0.246
1.355ProArg: 1.355 ± 0.411
2.935ProSer: 2.935 ± 0.351
3.085ProThr: 3.085 ± 0.486
2.107ProVal: 2.107 ± 0.37
0.376ProTrp: 0.376 ± 0.186
1.129ProTyr: 1.129 ± 0.386
0.0ProXaa: 0.0 ± 0.0
Gln
3.989GlnAla: 3.989 ± 0.539
0.301GlnCys: 0.301 ± 0.13
1.806GlnAsp: 1.806 ± 0.358
2.258GlnGlu: 2.258 ± 0.567
1.58GlnPhe: 1.58 ± 0.232
1.881GlnGly: 1.881 ± 0.396
0.903GlnHis: 0.903 ± 0.261
3.01GlnIle: 3.01 ± 0.452
2.634GlnLys: 2.634 ± 0.535
3.085GlnLeu: 3.085 ± 0.657
0.602GlnMet: 0.602 ± 0.181
2.258GlnAsn: 2.258 ± 0.444
0.828GlnPro: 0.828 ± 0.259
1.054GlnGln: 1.054 ± 0.337
1.054GlnArg: 1.054 ± 0.285
2.634GlnSer: 2.634 ± 0.415
2.709GlnThr: 2.709 ± 0.427
1.58GlnVal: 1.58 ± 0.205
0.226GlnTrp: 0.226 ± 0.123
1.054GlnTyr: 1.054 ± 0.313
0.0GlnXaa: 0.0 ± 0.0
Arg
1.957ArgAla: 1.957 ± 0.331
0.301ArgCys: 0.301 ± 0.187
2.784ArgAsp: 2.784 ± 0.386
1.957ArgGlu: 1.957 ± 0.447
2.333ArgPhe: 2.333 ± 0.437
2.333ArgGly: 2.333 ± 0.536
0.527ArgHis: 0.527 ± 0.175
2.709ArgIle: 2.709 ± 0.424
3.688ArgLys: 3.688 ± 0.796
3.537ArgLeu: 3.537 ± 0.53
0.903ArgMet: 0.903 ± 0.338
2.784ArgAsn: 2.784 ± 0.592
0.978ArgPro: 0.978 ± 0.252
1.054ArgGln: 1.054 ± 0.259
1.881ArgArg: 1.881 ± 0.464
3.161ArgSer: 3.161 ± 0.558
2.258ArgThr: 2.258 ± 0.385
1.656ArgVal: 1.656 ± 0.343
0.527ArgTrp: 0.527 ± 0.176
1.806ArgTyr: 1.806 ± 0.582
0.0ArgXaa: 0.0 ± 0.0
Ser
5.268SerAla: 5.268 ± 0.992
0.677SerCys: 0.677 ± 0.252
3.612SerAsp: 3.612 ± 0.384
3.462SerGlu: 3.462 ± 0.449
2.634SerPhe: 2.634 ± 0.489
7.601SerGly: 7.601 ± 1.198
1.656SerHis: 1.656 ± 0.371
5.644SerIle: 5.644 ± 0.52
5.268SerLys: 5.268 ± 0.671
4.967SerLeu: 4.967 ± 0.66
1.279SerMet: 1.279 ± 0.312
5.719SerAsn: 5.719 ± 0.851
1.355SerPro: 1.355 ± 0.247
2.483SerGln: 2.483 ± 0.383
2.258SerArg: 2.258 ± 0.361
6.321SerSer: 6.321 ± 0.998
5.117SerThr: 5.117 ± 1.192
4.892SerVal: 4.892 ± 0.852
0.978SerTrp: 0.978 ± 0.29
3.236SerTyr: 3.236 ± 0.505
0.0SerXaa: 0.0 ± 0.0
Thr
6.02ThrAla: 6.02 ± 0.943
0.527ThrCys: 0.527 ± 0.183
4.29ThrAsp: 4.29 ± 0.581
3.311ThrGlu: 3.311 ± 0.487
2.86ThrPhe: 2.86 ± 0.471
6.924ThrGly: 6.924 ± 1.297
1.204ThrHis: 1.204 ± 0.33
5.87ThrIle: 5.87 ± 0.712
5.494ThrLys: 5.494 ± 0.642
4.967ThrLeu: 4.967 ± 0.686
1.204ThrMet: 1.204 ± 0.362
5.343ThrAsn: 5.343 ± 0.834
2.107ThrPro: 2.107 ± 0.376
3.236ThrGln: 3.236 ± 0.519
2.333ThrArg: 2.333 ± 0.448
5.343ThrSer: 5.343 ± 1.317
6.472ThrThr: 6.472 ± 1.193
4.214ThrVal: 4.214 ± 1.07
0.828ThrTrp: 0.828 ± 0.243
2.032ThrTyr: 2.032 ± 0.488
0.0ThrXaa: 0.0 ± 0.0
Val
5.87ValAla: 5.87 ± 1.459
0.376ValCys: 0.376 ± 0.197
3.763ValAsp: 3.763 ± 0.591
3.311ValGlu: 3.311 ± 0.492
1.806ValPhe: 1.806 ± 0.295
3.387ValGly: 3.387 ± 0.562
0.828ValHis: 0.828 ± 0.283
3.913ValIle: 3.913 ± 0.494
4.29ValLys: 4.29 ± 0.611
3.01ValLeu: 3.01 ± 0.515
1.129ValMet: 1.129 ± 0.265
4.214ValAsn: 4.214 ± 0.735
1.957ValPro: 1.957 ± 0.341
1.881ValGln: 1.881 ± 0.3
2.333ValArg: 2.333 ± 0.459
5.117ValSer: 5.117 ± 0.627
4.214ValThr: 4.214 ± 0.528
2.258ValVal: 2.258 ± 0.444
0.226ValTrp: 0.226 ± 0.133
2.107ValTyr: 2.107 ± 0.349
0.0ValXaa: 0.0 ± 0.0
Trp
0.828TrpAla: 0.828 ± 0.225
0.151TrpCys: 0.151 ± 0.101
0.527TrpAsp: 0.527 ± 0.174
0.677TrpGlu: 0.677 ± 0.241
0.376TrpPhe: 0.376 ± 0.198
0.527TrpGly: 0.527 ± 0.222
0.226TrpHis: 0.226 ± 0.124
1.129TrpIle: 1.129 ± 0.27
1.129TrpLys: 1.129 ± 0.313
1.806TrpLeu: 1.806 ± 0.422
0.075TrpMet: 0.075 ± 0.076
0.602TrpAsn: 0.602 ± 0.181
0.376TrpPro: 0.376 ± 0.145
0.527TrpGln: 0.527 ± 0.213
0.527TrpArg: 0.527 ± 0.258
0.753TrpSer: 0.753 ± 0.276
0.903TrpThr: 0.903 ± 0.251
0.602TrpVal: 0.602 ± 0.24
0.075TrpTrp: 0.075 ± 0.076
0.226TrpTyr: 0.226 ± 0.116
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.634TyrAla: 2.634 ± 0.381
0.376TyrCys: 0.376 ± 0.175
2.032TyrAsp: 2.032 ± 0.403
1.505TyrGlu: 1.505 ± 0.224
1.505TyrPhe: 1.505 ± 0.37
2.86TyrGly: 2.86 ± 0.425
0.677TyrHis: 0.677 ± 0.251
3.01TyrIle: 3.01 ± 0.475
2.333TyrLys: 2.333 ± 0.384
3.236TyrLeu: 3.236 ± 0.494
0.452TyrMet: 0.452 ± 0.171
2.408TyrAsn: 2.408 ± 0.485
1.204TyrPro: 1.204 ± 0.29
1.43TyrGln: 1.43 ± 0.389
1.129TyrArg: 1.129 ± 0.26
2.86TyrSer: 2.86 ± 0.544
2.935TyrThr: 2.935 ± 0.535
2.86TyrVal: 2.86 ± 0.447
0.677TyrTrp: 0.677 ± 0.218
1.731TyrTyr: 1.731 ± 0.351
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (13289 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski