Arthrobacter phage Vibaki

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vibakivirus; Arthrobacter virus Vibaki

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514TYW6|A0A514TYW6_9CAUD Portal protein OS=Arthrobacter phage Vibaki OX=2593333 GN=5 PE=4 SV=1
MM1 pKa = 6.83TTTFEE6 pKa = 4.01QARR9 pKa = 11.84KK10 pKa = 9.56IGYY13 pKa = 9.15DD14 pKa = 3.38LNAPAWRR21 pKa = 11.84DD22 pKa = 3.17LGNPGEE28 pKa = 4.33YY29 pKa = 9.18MVADD33 pKa = 3.94YY34 pKa = 10.52GYY36 pKa = 9.78EE37 pKa = 4.05DD38 pKa = 3.41AAAWLLVDD46 pKa = 4.38GARR49 pKa = 11.84EE50 pKa = 3.84LLEE53 pKa = 4.89GGDD56 pKa = 3.77YY57 pKa = 11.29AFGVLDD63 pKa = 3.66QPLTLVLKK71 pKa = 9.75ATGDD75 pKa = 3.81VLQVQYY81 pKa = 11.46LEE83 pKa = 4.27AQDD86 pKa = 5.0MIDD89 pKa = 3.59AMTPVGDD96 pKa = 4.4VPPSDD101 pKa = 5.58DD102 pKa = 3.75PDD104 pKa = 3.28AA105 pKa = 6.17

Molecular weight: 11.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514TYY5|A0A514TYY5_9CAUD Uncharacterized protein OS=Arthrobacter phage Vibaki OX=2593333 GN=22 PE=4 SV=1
MM1 pKa = 7.08VRR3 pKa = 11.84VLVARR8 pKa = 11.84TQAAATMAVTTDD20 pKa = 3.09DD21 pKa = 3.56GKK23 pKa = 11.1RR24 pKa = 11.84YY25 pKa = 10.05SLYY28 pKa = 10.66SSPSQFDD35 pKa = 3.32FGEE38 pKa = 4.25VARR41 pKa = 11.84FGQIDD46 pKa = 3.91RR47 pKa = 11.84EE48 pKa = 4.19GLKK51 pKa = 10.61PITRR55 pKa = 11.84NVGAGLRR62 pKa = 11.84VMSFTHH68 pKa = 6.48RR69 pKa = 11.84VGSRR73 pKa = 11.84DD74 pKa = 3.46YY75 pKa = 10.55QQSIEE80 pKa = 4.33HH81 pKa = 6.56ALKK84 pKa = 10.51PLTDD88 pKa = 3.81LAKK91 pKa = 10.0NGQRR95 pKa = 11.84VRR97 pKa = 11.84FVGGSTQYY105 pKa = 10.84EE106 pKa = 3.86QGVWWNIKK114 pKa = 9.31GLAVKK119 pKa = 8.48VTQRR123 pKa = 11.84ALDD126 pKa = 3.67NRR128 pKa = 11.84ISRR131 pKa = 11.84AEE133 pKa = 3.96LSWDD137 pKa = 3.22LEE139 pKa = 4.26EE140 pKa = 5.63AVDD143 pKa = 3.72VTTNLIRR150 pKa = 11.84VIPKK154 pKa = 10.07RR155 pKa = 11.84PAPKK159 pKa = 9.31PAPPAARR166 pKa = 11.84QHH168 pKa = 5.9RR169 pKa = 11.84VVPGDD174 pKa = 3.9TLWHH178 pKa = 5.41IAARR182 pKa = 11.84YY183 pKa = 7.18LHH185 pKa = 6.69NGARR189 pKa = 11.84WPEE192 pKa = 3.91IFRR195 pKa = 11.84LNQGQIRR202 pKa = 11.84NPHH205 pKa = 6.81WIYY208 pKa = 10.07PGQVFKK214 pKa = 11.07IPARR218 pKa = 3.81

Molecular weight: 24.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

15172

35

1049

194.5

20.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.388 ± 0.552

0.685 ± 0.116

6.341 ± 0.197

5.378 ± 0.359

2.748 ± 0.238

8.325 ± 0.4

1.911 ± 0.185

4.245 ± 0.216

3.935 ± 0.256

7.922 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.037 ± 0.128

2.907 ± 0.187

6.341 ± 0.375

3.315 ± 0.212

6.294 ± 0.411

4.666 ± 0.314

7.494 ± 0.369

6.947 ± 0.283

1.615 ± 0.152

2.505 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski