Octadecabacter arcticus 238
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4331 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M9RE32|M9RE32_9RHOB IS5 family transposase OS=Octadecabacter arcticus 238 OX=391616 GN=OA238_c06370 PE=4 SV=1
MM1 pKa = 6.75 NTNLILAAVIAGFVATTVAAQTTTFDD27 pKa = 3.58 NLGAAEE33 pKa = 4.45 TFNDD37 pKa = 4.37 DD38 pKa = 4.86 LDD40 pKa = 4.02 EE41 pKa = 4.54 QMEE44 pKa = 4.38 DD45 pKa = 4.41 DD46 pKa = 4.78 RR47 pKa = 11.84 DD48 pKa = 3.57 RR49 pKa = 11.84 DD50 pKa = 3.51 LTGFGTEE57 pKa = 3.87 GRR59 pKa = 11.84 EE60 pKa = 3.85 LGDD63 pKa = 3.43 YY64 pKa = 11.09 GSIALRR70 pKa = 11.84 YY71 pKa = 7.64 TSTSNDD77 pKa = 2.83 GDD79 pKa = 3.71 TSNDD83 pKa = 3.13 LGVGLRR89 pKa = 11.84 YY90 pKa = 9.98 GWFDD94 pKa = 3.3 GVNGIDD100 pKa = 3.54 TNASYY105 pKa = 10.83 AYY107 pKa = 10.04 GEE109 pKa = 4.26 NNGVISEE116 pKa = 3.86 NRR118 pKa = 11.84 FLGGVDD124 pKa = 3.31 YY125 pKa = 10.96 RR126 pKa = 11.84 RR127 pKa = 11.84 NLSDD131 pKa = 4.07 AFFAYY136 pKa = 9.88 GQTDD140 pKa = 3.28 VSIDD144 pKa = 3.57 KK145 pKa = 8.69 QTTTAGEE152 pKa = 4.03 YY153 pKa = 8.73 TQDD156 pKa = 2.62 IFAGVGVGYY165 pKa = 10.59 RR166 pKa = 11.84 IFNSNDD172 pKa = 3.64 TQWSIQAGPGYY183 pKa = 10.04 RR184 pKa = 11.84 AAEE187 pKa = 4.27 VVGGATVNEE196 pKa = 4.12 AAASISSNLYY206 pKa = 10.42 ASLTDD211 pKa = 3.33 TVYY214 pKa = 9.28 ITNDD218 pKa = 2.77 TDD220 pKa = 4.1 VIYY223 pKa = 11.02 SDD225 pKa = 4.78 FATTLSNDD233 pKa = 3.06 LALNVALTDD242 pKa = 3.64 TLALRR247 pKa = 11.84 TSYY250 pKa = 9.63 ATRR253 pKa = 11.84 FNDD256 pKa = 3.15 QTDD259 pKa = 3.17 NSFSDD264 pKa = 3.8 GEE266 pKa = 4.33 NTFGVSAVYY275 pKa = 10.64 NFNN278 pKa = 3.55
Molecular weight: 29.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.452
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.503
Grimsley 3.35
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.808
Patrickios 1.125
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.743
Protein with the highest isoelectric point:
>tr|M9RGS9|M9RGS9_9RHOB Integrase catalytic domain-containing protein OS=Octadecabacter arcticus 238 OX=391616 GN=OA238_c12210 PE=4 SV=1
MM1 pKa = 8.34 DD2 pKa = 4.25 IRR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 9.93 HH7 pKa = 6.07 LSSEE11 pKa = 3.86 DD12 pKa = 3.15 RR13 pKa = 11.84 GVILAEE19 pKa = 4.21 HH20 pKa = 6.36 NRR22 pKa = 11.84 GSSQRR27 pKa = 11.84 LIGQLLHH34 pKa = 6.95 RR35 pKa = 11.84 PASTICRR42 pKa = 11.84 EE43 pKa = 4.13 LARR46 pKa = 11.84 GRR48 pKa = 11.84 QEE50 pKa = 4.48 DD51 pKa = 4.15 GSYY54 pKa = 10.62 CPQAARR60 pKa = 11.84 QAYY63 pKa = 7.03 DD64 pKa = 2.97 ARR66 pKa = 11.84 RR67 pKa = 11.84 ARR69 pKa = 11.84 CRR71 pKa = 11.84 RR72 pKa = 11.84 KK73 pKa = 9.56 RR74 pKa = 11.84 KK75 pKa = 9.44 LVEE78 pKa = 4.11 GSDD81 pKa = 3.82 LYY83 pKa = 11.25 RR84 pKa = 11.84 FVHH87 pKa = 6.3 GKK89 pKa = 9.4 LVHH92 pKa = 5.93 LHH94 pKa = 6.6 WSPEE98 pKa = 3.89 QIAQRR103 pKa = 11.84 LRR105 pKa = 11.84 LMKK108 pKa = 10.14 PDD110 pKa = 4.58 DD111 pKa = 4.2 PSAHH115 pKa = 5.99 VSHH118 pKa = 6.07 EE119 pKa = 4.26 TIYY122 pKa = 11.06 AAIYY126 pKa = 7.38 AQPRR130 pKa = 11.84 GGLKK134 pKa = 10.31 AAMIEE139 pKa = 4.24 ALRR142 pKa = 11.84 QAKK145 pKa = 9.43 PKK147 pKa = 10.28 RR148 pKa = 11.84 GLKK151 pKa = 9.98 RR152 pKa = 11.84 RR153 pKa = 11.84 TAAGSAMVPEE163 pKa = 4.6 SLRR166 pKa = 11.84 IINRR170 pKa = 11.84 PEE172 pKa = 3.84 EE173 pKa = 4.1 IEE175 pKa = 3.91 ARR177 pKa = 11.84 LVPGHH182 pKa = 6.66 WEE184 pKa = 3.64 GDD186 pKa = 3.97 LIKK189 pKa = 10.82 GAFNRR194 pKa = 11.84 SSVGTLVEE202 pKa = 4.03 RR203 pKa = 11.84 KK204 pKa = 7.03 TRR206 pKa = 11.84 FVILCKK212 pKa = 9.55 MDD214 pKa = 3.6 GNGAEE219 pKa = 4.1 AALDD223 pKa = 3.53 SFTRR227 pKa = 11.84 QMRR230 pKa = 11.84 RR231 pKa = 11.84 LPADD235 pKa = 3.26 LRR237 pKa = 11.84 KK238 pKa = 10.67 SMTYY242 pKa = 10.82 DD243 pKa = 2.89 RR244 pKa = 11.84 GSEE247 pKa = 4.18 MACHH251 pKa = 6.6 RR252 pKa = 11.84 EE253 pKa = 3.42 LARR256 pKa = 11.84 RR257 pKa = 11.84 LKK259 pKa = 10.26 IDD261 pKa = 2.7 IWFCDD266 pKa = 3.71 PHH268 pKa = 8.49 APWQRR273 pKa = 11.84 GSNEE277 pKa = 3.8 NTNGLLRR284 pKa = 11.84 QFMPKK289 pKa = 9.23 GTDD292 pKa = 3.31 LNGASQTWLNDD303 pKa = 3.07 VANLMNNRR311 pKa = 11.84 PRR313 pKa = 11.84 KK314 pKa = 8.21 NPRR317 pKa = 11.84 MEE319 pKa = 3.88 NTRR322 pKa = 3.44
Molecular weight: 36.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.531
IPC_protein 10.35
Toseland 10.716
ProMoST 10.526
Dawson 10.789
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 10.935
Grimsley 10.818
Solomon 10.935
Lehninger 10.891
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.628
IPC_peptide 10.935
IPC2_peptide 9.619
IPC2.peptide.svr19 8.737
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4331
0
4331
1328656
26
1532
306.8
33.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.498 ± 0.048
1.014 ± 0.012
6.279 ± 0.031
5.372 ± 0.029
3.809 ± 0.022
8.204 ± 0.034
2.233 ± 0.022
5.684 ± 0.026
3.854 ± 0.033
9.574 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.913 ± 0.017
2.995 ± 0.017
4.672 ± 0.022
3.384 ± 0.021
6.311 ± 0.033
5.414 ± 0.027
5.876 ± 0.03
7.211 ± 0.027
1.425 ± 0.016
2.277 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here