Helicobacter canis NCTC 12740
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1800 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V8CES6|V8CES6_9HELI Uncharacterized protein OS=Helicobacter canis NCTC 12740 OX=1357399 GN=HMPREF2087_01415 PE=4 SV=1
MM1 pKa = 7.52 AVLHH5 pKa = 6.94 DD6 pKa = 3.87 VLNNSDD12 pKa = 4.41 YY13 pKa = 11.05 MLDD16 pKa = 3.6 LCADD20 pKa = 3.75 KK21 pKa = 10.96 SLTMADD27 pKa = 4.25 LDD29 pKa = 3.95 SSSVFICRR37 pKa = 11.84 CC38 pKa = 3.23
Molecular weight: 4.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 4.05
IPC_protein 3.897
Toseland 3.668
ProMoST 4.139
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.986
Rodwell 3.732
Grimsley 3.592
Solomon 3.91
Lehninger 3.872
Nozaki 4.101
DTASelect 4.406
Thurlkill 3.795
EMBOSS 3.986
Sillero 4.037
Patrickios 0.604
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|V8CIC0|V8CIC0_9HELI Single-stranded-DNA-specific exonuclease RecJ OS=Helicobacter canis NCTC 12740 OX=1357399 GN=HMPREF2087_00061 PE=3 SV=1
MM1 pKa = 8.13 DD2 pKa = 4.43 SRR4 pKa = 11.84 VLTMDD9 pKa = 3.2 YY10 pKa = 8.63 HH11 pKa = 6.11 TLQSKK16 pKa = 9.85 ARR18 pKa = 11.84 NDD20 pKa = 3.4 DD21 pKa = 3.65 TNADD25 pKa = 3.31 SSMRR29 pKa = 11.84 WILAPSFPAHH39 pKa = 6.95 APRR42 pKa = 11.84 ISPHH46 pKa = 5.04 YY47 pKa = 8.8 LKK49 pKa = 10.82 HH50 pKa = 5.87 SATLRR55 pKa = 11.84 YY56 pKa = 8.88 QALLALQRR64 pKa = 11.84 TKK66 pKa = 10.48 PCHH69 pKa = 5.1 HH70 pKa = 6.5 TQANTAHH77 pKa = 7.35 KK78 pKa = 9.18 PQNLPPKK85 pKa = 8.49 HH86 pKa = 5.92 RR87 pKa = 11.84 HH88 pKa = 5.86 IITLGIGANLGTRR101 pKa = 11.84 QEE103 pKa = 3.71 ICKK106 pKa = 10.23 RR107 pKa = 11.84 FAHH110 pKa = 6.53 LLDD113 pKa = 4.14 SLTSKK118 pKa = 11.01 DD119 pKa = 3.57 FTLLATSPILHH130 pKa = 6.53 NPPFGYY136 pKa = 9.39 TNQPYY141 pKa = 9.81 FYY143 pKa = 10.14 NATITLATSLSVVEE157 pKa = 3.94 LFARR161 pKa = 11.84 VFYY164 pKa = 10.57 LEE166 pKa = 3.98 RR167 pKa = 11.84 RR168 pKa = 11.84 FGRR171 pKa = 11.84 GRR173 pKa = 11.84 KK174 pKa = 8.84 RR175 pKa = 11.84 AFKK178 pKa = 10.15 NAPRR182 pKa = 11.84 TLDD185 pKa = 3.06 IDD187 pKa = 3.61 IIFYY191 pKa = 10.95 DD192 pKa = 3.44 KK193 pKa = 11.21 VIIKK197 pKa = 10.1 RR198 pKa = 11.84 PYY200 pKa = 8.8 LTIPHH205 pKa = 6.57 HH206 pKa = 6.03 GFRR209 pKa = 11.84 TRR211 pKa = 11.84 DD212 pKa = 3.49 SVLLPLGFMLKK223 pKa = 9.89 QVLEE227 pKa = 4.53 SKK229 pKa = 10.53 SIHH232 pKa = 5.65 QSTRR236 pKa = 11.84 SKK238 pKa = 10.28 PCRR241 pKa = 11.84 PLLSQEE247 pKa = 4.33 KK248 pKa = 9.67 LQQKK252 pKa = 8.89 HH253 pKa = 4.35 SKK255 pKa = 9.07 RR256 pKa = 11.84 RR257 pKa = 11.84 AMSS260 pKa = 3.28
Molecular weight: 29.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.809
IPC_protein 10.482
Toseland 10.672
ProMoST 10.321
Dawson 10.789
Bjellqvist 10.467
Wikipedia 10.979
Rodwell 11.125
Grimsley 10.833
Solomon 10.862
Lehninger 10.833
Nozaki 10.657
DTASelect 10.467
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.716
Patrickios 10.818
IPC_peptide 10.862
IPC2_peptide 9.355
IPC2.peptide.svr19 8.567
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1800
0
1800
596658
29
2885
331.5
36.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.754 ± 0.056
1.381 ± 0.025
5.092 ± 0.037
5.991 ± 0.061
4.744 ± 0.046
6.191 ± 0.056
2.146 ± 0.028
7.438 ± 0.05
6.641 ± 0.063
10.781 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.111 ± 0.027
4.047 ± 0.041
3.645 ± 0.037
4.247 ± 0.039
4.293 ± 0.035
7.845 ± 0.055
4.654 ± 0.036
5.537 ± 0.052
0.789 ± 0.017
3.67 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here