Amino acid dipepetide frequency for Variovorax sp. OV329

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.235AlaAla: 18.235 ± 0.142
1.293AlaCys: 1.293 ± 0.027
6.368AlaAsp: 6.368 ± 0.061
6.39AlaGlu: 6.39 ± 0.071
4.294AlaPhe: 4.294 ± 0.048
11.443AlaGly: 11.443 ± 0.105
2.647AlaHis: 2.647 ± 0.034
5.25AlaIle: 5.25 ± 0.053
3.862AlaLys: 3.862 ± 0.058
15.009AlaLeu: 15.009 ± 0.117
3.759AlaMet: 3.759 ± 0.049
2.852AlaAsn: 2.852 ± 0.041
6.761AlaPro: 6.761 ± 0.08
6.25AlaGln: 6.25 ± 0.068
9.469AlaArg: 9.469 ± 0.076
7.3AlaSer: 7.3 ± 0.075
6.13AlaThr: 6.13 ± 0.07
9.116AlaVal: 9.116 ± 0.081
2.016AlaTrp: 2.016 ± 0.037
2.484AlaTyr: 2.484 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.186CysAla: 1.186 ± 0.027
0.115CysCys: 0.115 ± 0.007
0.493CysAsp: 0.493 ± 0.018
0.543CysGlu: 0.543 ± 0.02
0.304CysPhe: 0.304 ± 0.013
0.957CysGly: 0.957 ± 0.027
0.246CysHis: 0.246 ± 0.012
0.406CysIle: 0.406 ± 0.015
0.219CysLys: 0.219 ± 0.012
0.79CysLeu: 0.79 ± 0.019
0.197CysMet: 0.197 ± 0.011
0.217CysAsn: 0.217 ± 0.01
0.422CysPro: 0.422 ± 0.017
0.255CysGln: 0.255 ± 0.012
0.556CysArg: 0.556 ± 0.021
0.488CysSer: 0.488 ± 0.017
0.466CysThr: 0.466 ± 0.018
0.711CysVal: 0.711 ± 0.023
0.121CysTrp: 0.121 ± 0.007
0.19CysTyr: 0.19 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.387AspAla: 7.387 ± 0.065
0.446AspCys: 0.446 ± 0.017
2.573AspAsp: 2.573 ± 0.046
3.276AspGlu: 3.276 ± 0.043
2.076AspPhe: 2.076 ± 0.03
4.523AspGly: 4.523 ± 0.055
1.031AspHis: 1.031 ± 0.024
2.274AspIle: 2.274 ± 0.035
1.656AspLys: 1.656 ± 0.031
5.197AspLeu: 5.197 ± 0.054
1.225AspMet: 1.225 ± 0.027
1.19AspAsn: 1.19 ± 0.026
2.949AspPro: 2.949 ± 0.045
1.462AspGln: 1.462 ± 0.031
3.297AspArg: 3.297 ± 0.048
2.386AspSer: 2.386 ± 0.033
2.42AspThr: 2.42 ± 0.039
3.815AspVal: 3.815 ± 0.043
0.914AspTrp: 0.914 ± 0.023
1.336AspTyr: 1.336 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
6.971GluAla: 6.971 ± 0.068
0.404GluCys: 0.404 ± 0.015
2.336GluAsp: 2.336 ± 0.04
2.699GluGlu: 2.699 ± 0.047
1.774GluPhe: 1.774 ± 0.028
4.104GluGly: 4.104 ± 0.047
1.339GluHis: 1.339 ± 0.029
2.529GluIle: 2.529 ± 0.037
1.827GluLys: 1.827 ± 0.037
6.03GluLeu: 6.03 ± 0.06
1.294GluMet: 1.294 ± 0.026
1.188GluAsn: 1.188 ± 0.026
2.586GluPro: 2.586 ± 0.04
2.47GluGln: 2.47 ± 0.044
4.772GluArg: 4.772 ± 0.061
2.672GluSer: 2.672 ± 0.037
2.222GluThr: 2.222 ± 0.036
4.127GluVal: 4.127 ± 0.054
0.703GluTrp: 0.703 ± 0.019
0.948GluTyr: 0.948 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
4.407PheAla: 4.407 ± 0.056
0.365PheCys: 0.365 ± 0.012
2.555PheAsp: 2.555 ± 0.039
2.153PheGlu: 2.153 ± 0.031
1.342PhePhe: 1.342 ± 0.033
3.498PheGly: 3.498 ± 0.046
0.712PheHis: 0.712 ± 0.019
1.466PheIle: 1.466 ± 0.032
1.135PheLys: 1.135 ± 0.027
2.947PheLeu: 2.947 ± 0.047
0.849PheMet: 0.849 ± 0.025
1.092PheAsn: 1.092 ± 0.022
1.538PhePro: 1.538 ± 0.029
1.046PheGln: 1.046 ± 0.025
1.948PheArg: 1.948 ± 0.035
2.117PheSer: 2.117 ± 0.031
1.823PheThr: 1.823 ± 0.034
2.879PheVal: 2.879 ± 0.046
0.535PheTrp: 0.535 ± 0.016
0.837PheTyr: 0.837 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
10.134GlyAla: 10.134 ± 0.111
0.872GlyCys: 0.872 ± 0.024
3.931GlyAsp: 3.931 ± 0.044
4.581GlyGlu: 4.581 ± 0.056
3.33GlyPhe: 3.33 ± 0.048
7.676GlyGly: 7.676 ± 0.119
1.91GlyHis: 1.91 ± 0.035
4.018GlyIle: 4.018 ± 0.053
3.242GlyLys: 3.242 ± 0.043
8.965GlyLeu: 8.965 ± 0.083
2.391GlyMet: 2.391 ± 0.036
2.274GlyAsn: 2.274 ± 0.046
3.33GlyPro: 3.33 ± 0.042
3.428GlyGln: 3.428 ± 0.04
5.875GlyArg: 5.875 ± 0.064
5.002GlySer: 5.002 ± 0.066
4.763GlyThr: 4.763 ± 0.071
6.668GlyVal: 6.668 ± 0.059
1.465GlyTrp: 1.465 ± 0.029
2.295GlyTyr: 2.295 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.898HisAla: 2.898 ± 0.04
0.274HisCys: 0.274 ± 0.01
1.154HisAsp: 1.154 ± 0.025
1.221HisGlu: 1.221 ± 0.023
0.877HisPhe: 0.877 ± 0.02
2.12HisGly: 2.12 ± 0.041
0.599HisHis: 0.599 ± 0.017
0.861HisIle: 0.861 ± 0.022
0.542HisLys: 0.542 ± 0.018
2.173HisLeu: 2.173 ± 0.036
0.524HisMet: 0.524 ± 0.017
0.467HisAsn: 0.467 ± 0.016
1.48HisPro: 1.48 ± 0.027
0.678HisGln: 0.678 ± 0.02
1.505HisArg: 1.505 ± 0.029
1.025HisSer: 1.025 ± 0.022
0.904HisThr: 0.904 ± 0.02
1.597HisVal: 1.597 ± 0.03
0.425HisTrp: 0.425 ± 0.016
0.604HisTyr: 0.604 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
6.07IleAla: 6.07 ± 0.054
0.393IleCys: 0.393 ± 0.015
3.044IleAsp: 3.044 ± 0.042
3.079IleGlu: 3.079 ± 0.047
1.276IlePhe: 1.276 ± 0.028
4.205IleGly: 4.205 ± 0.054
0.847IleHis: 0.847 ± 0.022
1.33IleIle: 1.33 ± 0.029
1.304IleLys: 1.304 ± 0.028
3.105IleLeu: 3.105 ± 0.05
0.666IleMet: 0.666 ± 0.022
1.324IleAsn: 1.324 ± 0.029
1.932IlePro: 1.932 ± 0.034
1.225IleGln: 1.225 ± 0.025
2.588IleArg: 2.588 ± 0.039
2.326IleSer: 2.326 ± 0.036
2.204IleThr: 2.204 ± 0.034
3.546IleVal: 3.546 ± 0.046
0.476IleTrp: 0.476 ± 0.014
0.943IleTyr: 0.943 ± 0.021
0.0IleXaa: 0.0 ± 0.0
Lys
4.078LysAla: 4.078 ± 0.061
0.155LysCys: 0.155 ± 0.009
1.694LysAsp: 1.694 ± 0.036
1.547LysGlu: 1.547 ± 0.031
0.879LysPhe: 0.879 ± 0.026
2.468LysGly: 2.468 ± 0.043
0.567LysHis: 0.567 ± 0.02
1.326LysIle: 1.326 ± 0.028
1.365LysLys: 1.365 ± 0.043
3.482LysLeu: 3.482 ± 0.052
0.743LysMet: 0.743 ± 0.021
0.863LysAsn: 0.863 ± 0.024
2.057LysPro: 2.057 ± 0.036
1.061LysGln: 1.061 ± 0.024
2.095LysArg: 2.095 ± 0.035
1.793LysSer: 1.793 ± 0.033
1.785LysThr: 1.785 ± 0.029
2.559LysVal: 2.559 ± 0.04
0.379LysTrp: 0.379 ± 0.013
0.614LysTyr: 0.614 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
15.418LeuAla: 15.418 ± 0.111
0.97LeuCys: 0.97 ± 0.024
5.726LeuAsp: 5.726 ± 0.056
5.11LeuGlu: 5.11 ± 0.058
3.451LeuPhe: 3.451 ± 0.046
8.97LeuGly: 8.97 ± 0.08
2.354LeuHis: 2.354 ± 0.035
4.048LeuIle: 4.048 ± 0.051
3.366LeuLys: 3.366 ± 0.05
11.2LeuLeu: 11.2 ± 0.108
2.57LeuMet: 2.57 ± 0.043
2.512LeuAsn: 2.512 ± 0.037
6.235LeuPro: 6.235 ± 0.064
4.181LeuGln: 4.181 ± 0.052
7.873LeuArg: 7.873 ± 0.076
6.151LeuSer: 6.151 ± 0.054
4.808LeuThr: 4.808 ± 0.055
8.205LeuVal: 8.205 ± 0.083
1.31LeuTrp: 1.31 ± 0.032
1.957LeuTyr: 1.957 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
3.155MetAla: 3.155 ± 0.044
0.192MetCys: 0.192 ± 0.01
1.111MetAsp: 1.111 ± 0.024
1.063MetGlu: 1.063 ± 0.022
0.737MetPhe: 0.737 ± 0.019
1.961MetGly: 1.961 ± 0.029
0.57MetHis: 0.57 ± 0.016
0.922MetIle: 0.922 ± 0.024
1.009MetLys: 1.009 ± 0.024
2.727MetLeu: 2.727 ± 0.043
0.539MetMet: 0.539 ± 0.018
0.881MetAsn: 0.881 ± 0.019
1.597MetPro: 1.597 ± 0.03
1.098MetGln: 1.098 ± 0.023
1.895MetArg: 1.895 ± 0.03
1.758MetSer: 1.758 ± 0.024
1.491MetThr: 1.491 ± 0.03
1.737MetVal: 1.737 ± 0.026
0.212MetTrp: 0.212 ± 0.012
0.375MetTyr: 0.375 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.227AsnAla: 3.227 ± 0.052
0.251AsnCys: 0.251 ± 0.011
1.239AsnAsp: 1.239 ± 0.03
1.251AsnGlu: 1.251 ± 0.027
0.987AsnPhe: 0.987 ± 0.023
2.22AsnGly: 2.22 ± 0.047
0.471AsnHis: 0.471 ± 0.015
1.156AsnIle: 1.156 ± 0.026
0.79AsnLys: 0.79 ± 0.025
2.499AsnLeu: 2.499 ± 0.04
0.569AsnMet: 0.569 ± 0.019
0.74AsnAsn: 0.74 ± 0.023
1.766AsnPro: 1.766 ± 0.032
0.798AsnGln: 0.798 ± 0.021
1.515AsnArg: 1.515 ± 0.026
1.304AsnSer: 1.304 ± 0.029
1.407AsnThr: 1.407 ± 0.031
1.914AsnVal: 1.914 ± 0.031
0.421AsnTrp: 0.421 ± 0.015
0.658AsnTyr: 0.658 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.453ProAla: 7.453 ± 0.081
0.346ProCys: 0.346 ± 0.014
3.139ProAsp: 3.139 ± 0.045
3.345ProGlu: 3.345 ± 0.049
1.826ProPhe: 1.826 ± 0.029
4.913ProGly: 4.913 ± 0.06
1.134ProHis: 1.134 ± 0.024
1.94ProIle: 1.94 ± 0.035
1.515ProLys: 1.515 ± 0.034
5.327ProLeu: 5.327 ± 0.055
1.414ProMet: 1.414 ± 0.026
1.262ProAsn: 1.262 ± 0.024
2.932ProPro: 2.932 ± 0.044
2.27ProGln: 2.27 ± 0.037
3.154ProArg: 3.154 ± 0.047
2.934ProSer: 2.934 ± 0.042
2.621ProThr: 2.621 ± 0.039
4.344ProVal: 4.344 ± 0.048
0.839ProTrp: 0.839 ± 0.023
1.142ProTyr: 1.142 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
5.721GlnAla: 5.721 ± 0.072
0.288GlnCys: 0.288 ± 0.013
1.693GlnAsp: 1.693 ± 0.033
1.64GlnGlu: 1.64 ± 0.032
1.175GlnPhe: 1.175 ± 0.023
3.243GlnGly: 3.243 ± 0.043
0.873GlnHis: 0.873 ± 0.019
1.629GlnIle: 1.629 ± 0.031
1.138GlnLys: 1.138 ± 0.022
4.355GlnLeu: 4.355 ± 0.048
1.028GlnMet: 1.028 ± 0.026
0.798GlnAsn: 0.798 ± 0.02
2.261GlnPro: 2.261 ± 0.04
1.997GlnGln: 1.997 ± 0.079
3.391GlnArg: 3.391 ± 0.045
2.07GlnSer: 2.07 ± 0.036
1.745GlnThr: 1.745 ± 0.028
3.257GlnVal: 3.257 ± 0.044
0.657GlnTrp: 0.657 ± 0.017
0.716GlnTyr: 0.716 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
8.218ArgAla: 8.218 ± 0.073
0.615ArgCys: 0.615 ± 0.018
3.494ArgAsp: 3.494 ± 0.043
4.491ArgGlu: 4.491 ± 0.059
2.845ArgPhe: 2.845 ± 0.042
4.867ArgGly: 4.867 ± 0.054
1.856ArgHis: 1.856 ± 0.03
3.508ArgIle: 3.508 ± 0.04
2.146ArgLys: 2.146 ± 0.038
7.9ArgLeu: 7.9 ± 0.077
1.995ArgMet: 1.995 ± 0.035
1.836ArgAsn: 1.836 ± 0.033
3.422ArgPro: 3.422 ± 0.049
2.89ArgGln: 2.89 ± 0.048
5.343ArgArg: 5.343 ± 0.057
3.752ArgSer: 3.752 ± 0.044
3.264ArgThr: 3.264 ± 0.037
5.203ArgVal: 5.203 ± 0.057
1.221ArgTrp: 1.221 ± 0.026
1.833ArgTyr: 1.833 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.87SerAla: 6.87 ± 0.072
0.444SerCys: 0.444 ± 0.016
2.609SerAsp: 2.609 ± 0.035
2.586SerGlu: 2.586 ± 0.038
2.219SerPhe: 2.219 ± 0.034
5.36SerGly: 5.36 ± 0.07
1.202SerHis: 1.202 ± 0.026
2.372SerIle: 2.372 ± 0.039
1.582SerLys: 1.582 ± 0.028
5.799SerLeu: 5.799 ± 0.055
1.425SerMet: 1.425 ± 0.026
1.44SerAsn: 1.44 ± 0.033
3.028SerPro: 3.028 ± 0.039
2.015SerGln: 2.015 ± 0.03
3.805SerArg: 3.805 ± 0.045
3.302SerSer: 3.302 ± 0.058
3.111SerThr: 3.111 ± 0.05
4.111SerVal: 4.111 ± 0.048
0.762SerTrp: 0.762 ± 0.019
1.308SerTyr: 1.308 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
5.731ThrAla: 5.731 ± 0.064
0.384ThrCys: 0.384 ± 0.013
2.329ThrAsp: 2.329 ± 0.036
2.259ThrGlu: 2.259 ± 0.038
1.605ThrPhe: 1.605 ± 0.029
4.609ThrGly: 4.609 ± 0.063
1.146ThrHis: 1.146 ± 0.024
2.02ThrIle: 2.02 ± 0.031
1.209ThrLys: 1.209 ± 0.031
5.996ThrLeu: 5.996 ± 0.066
1.048ThrMet: 1.048 ± 0.025
1.15ThrAsn: 1.15 ± 0.028
3.417ThrPro: 3.417 ± 0.046
1.981ThrGln: 1.981 ± 0.032
3.38ThrArg: 3.38 ± 0.04
2.662ThrSer: 2.662 ± 0.048
2.668ThrThr: 2.668 ± 0.052
4.162ThrVal: 4.162 ± 0.049
0.711ThrTrp: 0.711 ± 0.021
0.961ThrTyr: 0.961 ± 0.022
0.0ThrXaa: 0.0 ± 0.0
Val
9.844ValAla: 9.844 ± 0.073
0.687ValCys: 0.687 ± 0.021
4.152ValAsp: 4.152 ± 0.048
4.047ValGlu: 4.047 ± 0.054
2.766ValPhe: 2.766 ± 0.046
5.948ValGly: 5.948 ± 0.06
1.63ValHis: 1.63 ± 0.029
3.348ValIle: 3.348 ± 0.047
2.411ValLys: 2.411 ± 0.04
8.641ValLeu: 8.641 ± 0.088
1.908ValMet: 1.908 ± 0.034
2.082ValAsn: 2.082 ± 0.038
4.279ValPro: 4.279 ± 0.053
3.066ValGln: 3.066 ± 0.042
5.339ValArg: 5.339 ± 0.053
4.276ValSer: 4.276 ± 0.048
3.734ValThr: 3.734 ± 0.044
6.438ValVal: 6.438 ± 0.069
0.946ValTrp: 0.946 ± 0.023
1.518ValTyr: 1.518 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.375TrpAla: 1.375 ± 0.029
0.157TrpCys: 0.157 ± 0.009
0.619TrpAsp: 0.619 ± 0.02
0.558TrpGlu: 0.558 ± 0.016
0.542TrpPhe: 0.542 ± 0.017
1.046TrpGly: 1.046 ± 0.023
0.395TrpHis: 0.395 ± 0.015
0.693TrpIle: 0.693 ± 0.021
0.498TrpLys: 0.498 ± 0.016
2.003TrpLeu: 2.003 ± 0.035
0.439TrpMet: 0.439 ± 0.016
0.458TrpAsn: 0.458 ± 0.017
0.758TrpPro: 0.758 ± 0.018
0.711TrpGln: 0.711 ± 0.023
1.326TrpArg: 1.326 ± 0.028
0.85TrpSer: 0.85 ± 0.025
0.739TrpThr: 0.739 ± 0.018
1.011TrpVal: 1.011 ± 0.023
0.282TrpTrp: 0.282 ± 0.013
0.305TrpTyr: 0.305 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.552TyrAla: 2.552 ± 0.038
0.228TyrCys: 0.228 ± 0.011
1.176TyrAsp: 1.176 ± 0.027
1.154TyrGlu: 1.154 ± 0.027
0.938TyrPhe: 0.938 ± 0.022
2.017TyrGly: 2.017 ± 0.035
0.401TyrHis: 0.401 ± 0.014
0.79TyrIle: 0.79 ± 0.023
0.695TyrLys: 0.695 ± 0.022
2.269TyrLeu: 2.269 ± 0.039
0.448TyrMet: 0.448 ± 0.016
0.559TyrAsn: 0.559 ± 0.019
1.099TyrPro: 1.099 ± 0.025
0.794TyrGln: 0.794 ± 0.023
1.574TyrArg: 1.574 ± 0.027
1.179TyrSer: 1.179 ± 0.027
1.123TyrThr: 1.123 ± 0.026
1.661TyrVal: 1.661 ± 0.029
0.368TyrTrp: 0.368 ± 0.014
0.572TyrTyr: 0.572 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5872 proteins (1920499 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski