Japanese eel endothelial cells-infecting virus
Average proteome isoelectric point is 5.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7FIZ8|E7FIZ8_9VIRU Polyomavirus large T like protein OS=Japanese eel endothelial cells-infecting virus OX=712037 GN=LTLG PE=4 SV=1
MM1 pKa = 7.45 EE2 pKa = 5.32 GEE4 pKa = 4.16 EE5 pKa = 4.13 EE6 pKa = 3.85 DD7 pKa = 3.83 AEE9 pKa = 4.44 RR10 pKa = 11.84 NIYY13 pKa = 9.81 LQLNIFLFDD22 pKa = 4.41 DD23 pKa = 4.12 PSFVIRR29 pKa = 11.84 TYY31 pKa = 8.3 QTYY34 pKa = 10.71 KK35 pKa = 10.04 KK36 pKa = 9.69 AHH38 pKa = 6.5 KK39 pKa = 9.45 AACLRR44 pKa = 11.84 YY45 pKa = 9.65 HH46 pKa = 6.99 PDD48 pKa = 3.72 KK49 pKa = 10.78 NTSCPPEE56 pKa = 3.69 EE57 pKa = 4.11 RR58 pKa = 11.84 QRR60 pKa = 11.84 LEE62 pKa = 3.77 QRR64 pKa = 11.84 LKK66 pKa = 10.72 RR67 pKa = 11.84 INEE70 pKa = 3.62 VWAKK74 pKa = 10.87 YY75 pKa = 9.72 MDD77 pKa = 3.0 IHH79 pKa = 6.74 QRR81 pKa = 11.84 QQTWFDD87 pKa = 3.2 ISPSKK92 pKa = 10.72 RR93 pKa = 11.84 GGTLTVPEE101 pKa = 4.52 YY102 pKa = 10.25 LAKK105 pKa = 10.6 YY106 pKa = 7.49 CTTIQDD112 pKa = 3.64 GQFVQTVLVQVPWAKK127 pKa = 10.36 LGSVVTEE134 pKa = 3.95 LKK136 pKa = 10.38 KK137 pKa = 10.88 YY138 pKa = 10.38 KK139 pKa = 10.33 HH140 pKa = 6.53 VDD142 pKa = 4.59 LIAGGDD148 pKa = 3.76 PRR150 pKa = 11.84 EE151 pKa = 4.64 DD152 pKa = 3.87 PPTGIAVVQFVHH164 pKa = 6.1 RR165 pKa = 11.84 QKK167 pKa = 11.19 EE168 pKa = 4.13 SALKK172 pKa = 9.88 GKK174 pKa = 10.09 CNAVTLVCNVMQVANKK190 pKa = 10.4 SFTTVKK196 pKa = 10.15 QICLAYY202 pKa = 8.75 WKK204 pKa = 10.49 EE205 pKa = 3.69 NTTEE209 pKa = 4.21 HH210 pKa = 5.47 GHH212 pKa = 5.61 VMEE215 pKa = 6.25 DD216 pKa = 3.13 EE217 pKa = 4.38 TNPVGFDD224 pKa = 3.27 YY225 pKa = 11.5 TMLEE229 pKa = 3.95 QFACAIEE236 pKa = 4.41 CTDD239 pKa = 3.54 EE240 pKa = 3.91 LRR242 pKa = 11.84 LIGEE246 pKa = 4.5 YY247 pKa = 9.98 LACAYY252 pKa = 10.06 AVEE255 pKa = 4.86 DD256 pKa = 4.47 CFTCRR261 pKa = 11.84 SDD263 pKa = 3.41 QQKK266 pKa = 10.25 YY267 pKa = 9.71 AGSVYY272 pKa = 10.58 PEE274 pKa = 4.04 ASHH277 pKa = 7.03 IKK279 pKa = 10.09 LHH281 pKa = 5.76 AQHH284 pKa = 6.13 MVNAQFFKK292 pKa = 10.79 AVHH295 pKa = 5.8 NKK297 pKa = 9.72 KK298 pKa = 10.07 GQCQNATNTVIGIKK312 pKa = 9.56 RR313 pKa = 11.84 HH314 pKa = 5.3 SSASLSRR321 pKa = 11.84 QQQFEE326 pKa = 4.06 QRR328 pKa = 11.84 VLKK331 pKa = 10.45 ALKK334 pKa = 9.56 QVRR337 pKa = 11.84 QQIDD341 pKa = 3.35 TSKK344 pKa = 10.43 YY345 pKa = 8.24 AAAVALNHH353 pKa = 5.93 MLPLGMTVNDD363 pKa = 3.84 FCLKK367 pKa = 9.91 VLQRR371 pKa = 11.84 LITHH375 pKa = 6.4 EE376 pKa = 4.21 PKK378 pKa = 10.48 KK379 pKa = 10.77 SGIIFKK385 pKa = 11.08 GPMNTGKK392 pKa = 7.54 TTIAHH397 pKa = 6.73 AVLDD401 pKa = 4.13 MCSGTVLNMNGEE413 pKa = 4.18 MSQVRR418 pKa = 11.84 FEE420 pKa = 4.68 LGRR423 pKa = 11.84 ATGQFMVVFEE433 pKa = 5.07 DD434 pKa = 3.64 VTGVPEE440 pKa = 4.57 ADD442 pKa = 3.66 HH443 pKa = 7.21 PEE445 pKa = 4.09 LTAGAGMLNMDD456 pKa = 5.82 GIRR459 pKa = 11.84 DD460 pKa = 3.91 HH461 pKa = 7.25 LDD463 pKa = 3.19 CTVMVGLEE471 pKa = 3.65 RR472 pKa = 11.84 KK473 pKa = 9.51 YY474 pKa = 11.13 SNKK477 pKa = 8.95 VDD479 pKa = 3.4 QYY481 pKa = 9.6 FPPWIATMNEE491 pKa = 3.77 YY492 pKa = 10.53 KK493 pKa = 10.21 IPQGVSVRR501 pKa = 11.84 SEE503 pKa = 4.32 TYY505 pKa = 10.48 HH506 pKa = 4.43 MTRR509 pKa = 11.84 TISDD513 pKa = 3.55 LRR515 pKa = 11.84 QYY517 pKa = 10.77 LVSHH521 pKa = 6.38 NVQTRR526 pKa = 11.84 FMSSGQNLALMLALYY541 pKa = 10.79 ADD543 pKa = 3.83 ASYY546 pKa = 8.2 FTEE549 pKa = 3.97 EE550 pKa = 3.91 ALPIVEE556 pKa = 4.06 EE557 pKa = 4.08 LRR559 pKa = 11.84 RR560 pKa = 11.84 KK561 pKa = 10.09 GEE563 pKa = 4.01 EE564 pKa = 3.73 YY565 pKa = 10.53 DD566 pKa = 3.26 IGAMEE571 pKa = 3.79 QAIRR575 pKa = 11.84 GLRR578 pKa = 11.84 VDD580 pKa = 4.17 EE581 pKa = 4.34 SQEE584 pKa = 3.79 EE585 pKa = 4.03 EE586 pKa = 4.12 RR587 pKa = 11.84 EE588 pKa = 4.04 RR589 pKa = 11.84 EE590 pKa = 3.87 EE591 pKa = 4.68 EE592 pKa = 4.09 EE593 pKa = 4.79 FGNTQQFMSEE603 pKa = 4.1 TDD605 pKa = 3.25 RR606 pKa = 11.84 QRR608 pKa = 11.84 AGPASSNDD616 pKa = 3.0 FATASQEE623 pKa = 3.87 MGQAQQGSHH632 pKa = 5.34 TQPEE636 pKa = 4.9 INTGTQLSQSTHH648 pKa = 4.97 TADD651 pKa = 3.81 PEE653 pKa = 4.43 EE654 pKa = 4.83 GTSAPSQAKK663 pKa = 8.99 RR664 pKa = 11.84 RR665 pKa = 11.84 RR666 pKa = 11.84 RR667 pKa = 11.84 EE668 pKa = 3.89 PEE670 pKa = 3.74 SLQEE674 pKa = 4.27 LLLIDD679 pKa = 4.13 SDD681 pKa = 4.26 GMCILIIFSCSYY693 pKa = 10.98 LILFII698 pKa = 5.4
Molecular weight: 79.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.565
IPC2_protein 5.563
IPC_protein 5.588
Toseland 5.906
ProMoST 5.893
Dawson 5.804
Bjellqvist 5.83
Wikipedia 5.779
Rodwell 5.779
Grimsley 6.033
Solomon 5.804
Lehninger 5.779
Nozaki 6.046
DTASelect 6.224
Thurlkill 6.198
EMBOSS 6.16
Sillero 6.148
Patrickios 3.999
IPC_peptide 5.817
IPC2_peptide 6.148
IPC2.peptide.svr19 6.089
Protein with the highest isoelectric point:
>tr|E7FIZ8|E7FIZ8_9VIRU Polyomavirus large T like protein OS=Japanese eel endothelial cells-infecting virus OX=712037 GN=LTLG PE=4 SV=1
MM1 pKa = 7.45 EE2 pKa = 5.32 GEE4 pKa = 4.16 EE5 pKa = 4.13 EE6 pKa = 3.85 DD7 pKa = 3.83 AEE9 pKa = 4.44 RR10 pKa = 11.84 NIYY13 pKa = 9.81 LQLNIFLFDD22 pKa = 4.41 DD23 pKa = 4.12 PSFVIRR29 pKa = 11.84 TYY31 pKa = 8.3 QTYY34 pKa = 10.71 KK35 pKa = 10.04 KK36 pKa = 9.69 AHH38 pKa = 6.5 KK39 pKa = 9.45 AACLRR44 pKa = 11.84 YY45 pKa = 9.65 HH46 pKa = 6.99 PDD48 pKa = 3.72 KK49 pKa = 10.78 NTSCPPEE56 pKa = 3.69 EE57 pKa = 4.11 RR58 pKa = 11.84 QRR60 pKa = 11.84 LEE62 pKa = 3.77 QRR64 pKa = 11.84 LKK66 pKa = 10.72 RR67 pKa = 11.84 INEE70 pKa = 3.62 VWAKK74 pKa = 10.87 YY75 pKa = 9.72 MDD77 pKa = 3.0 IHH79 pKa = 6.74 QRR81 pKa = 11.84 QQTWFDD87 pKa = 3.2 ISPSKK92 pKa = 10.72 RR93 pKa = 11.84 GGTLTVPEE101 pKa = 4.52 YY102 pKa = 10.25 LAKK105 pKa = 10.6 YY106 pKa = 7.49 CTTIQDD112 pKa = 3.64 GQFVQTVLVQVPWAKK127 pKa = 10.36 LGSVVTEE134 pKa = 3.95 LKK136 pKa = 10.38 KK137 pKa = 10.88 YY138 pKa = 10.38 KK139 pKa = 10.33 HH140 pKa = 6.53 VDD142 pKa = 4.59 LIAGGDD148 pKa = 3.76 PRR150 pKa = 11.84 EE151 pKa = 4.64 DD152 pKa = 3.87 PPTGIAVVQFVHH164 pKa = 6.1 RR165 pKa = 11.84 QKK167 pKa = 11.19 EE168 pKa = 4.13 SALKK172 pKa = 9.88 GKK174 pKa = 10.09 CNAVTLVCNVMQVANKK190 pKa = 10.4 SFTTVKK196 pKa = 10.15 QICLAYY202 pKa = 8.75 WKK204 pKa = 10.49 EE205 pKa = 3.69 NTTEE209 pKa = 4.21 HH210 pKa = 5.47 GHH212 pKa = 5.61 VMEE215 pKa = 6.25 DD216 pKa = 3.13 EE217 pKa = 4.38 TNPVGFDD224 pKa = 3.27 YY225 pKa = 11.5 TMLEE229 pKa = 3.95 QFACAIEE236 pKa = 4.41 CTDD239 pKa = 3.54 EE240 pKa = 3.91 LRR242 pKa = 11.84 LIGEE246 pKa = 4.5 YY247 pKa = 9.98 LACAYY252 pKa = 10.06 AVEE255 pKa = 4.86 DD256 pKa = 4.47 CFTCRR261 pKa = 11.84 SDD263 pKa = 3.41 QQKK266 pKa = 10.25 YY267 pKa = 9.71 AGSVYY272 pKa = 10.58 PEE274 pKa = 4.04 ASHH277 pKa = 7.03 IKK279 pKa = 10.09 LHH281 pKa = 5.76 AQHH284 pKa = 6.13 MVNAQFFKK292 pKa = 10.79 AVHH295 pKa = 5.8 NKK297 pKa = 9.72 KK298 pKa = 10.07 GQCQNATNTVIGIKK312 pKa = 9.56 RR313 pKa = 11.84 HH314 pKa = 5.3 SSASLSRR321 pKa = 11.84 QQQFEE326 pKa = 4.06 QRR328 pKa = 11.84 VLKK331 pKa = 10.45 ALKK334 pKa = 9.56 QVRR337 pKa = 11.84 QQIDD341 pKa = 3.35 TSKK344 pKa = 10.43 YY345 pKa = 8.24 AAAVALNHH353 pKa = 5.93 MLPLGMTVNDD363 pKa = 3.84 FCLKK367 pKa = 9.91 VLQRR371 pKa = 11.84 LITHH375 pKa = 6.4 EE376 pKa = 4.21 PKK378 pKa = 10.48 KK379 pKa = 10.77 SGIIFKK385 pKa = 11.08 GPMNTGKK392 pKa = 7.54 TTIAHH397 pKa = 6.73 AVLDD401 pKa = 4.13 MCSGTVLNMNGEE413 pKa = 4.18 MSQVRR418 pKa = 11.84 FEE420 pKa = 4.68 LGRR423 pKa = 11.84 ATGQFMVVFEE433 pKa = 5.07 DD434 pKa = 3.64 VTGVPEE440 pKa = 4.57 ADD442 pKa = 3.66 HH443 pKa = 7.21 PEE445 pKa = 4.09 LTAGAGMLNMDD456 pKa = 5.82 GIRR459 pKa = 11.84 DD460 pKa = 3.91 HH461 pKa = 7.25 LDD463 pKa = 3.19 CTVMVGLEE471 pKa = 3.65 RR472 pKa = 11.84 KK473 pKa = 9.51 YY474 pKa = 11.13 SNKK477 pKa = 8.95 VDD479 pKa = 3.4 QYY481 pKa = 9.6 FPPWIATMNEE491 pKa = 3.77 YY492 pKa = 10.53 KK493 pKa = 10.21 IPQGVSVRR501 pKa = 11.84 SEE503 pKa = 4.32 TYY505 pKa = 10.48 HH506 pKa = 4.43 MTRR509 pKa = 11.84 TISDD513 pKa = 3.55 LRR515 pKa = 11.84 QYY517 pKa = 10.77 LVSHH521 pKa = 6.38 NVQTRR526 pKa = 11.84 FMSSGQNLALMLALYY541 pKa = 10.79 ADD543 pKa = 3.83 ASYY546 pKa = 8.2 FTEE549 pKa = 3.97 EE550 pKa = 3.91 ALPIVEE556 pKa = 4.06 EE557 pKa = 4.08 LRR559 pKa = 11.84 RR560 pKa = 11.84 KK561 pKa = 10.09 GEE563 pKa = 4.01 EE564 pKa = 3.73 YY565 pKa = 10.53 DD566 pKa = 3.26 IGAMEE571 pKa = 3.79 QAIRR575 pKa = 11.84 GLRR578 pKa = 11.84 VDD580 pKa = 4.17 EE581 pKa = 4.34 SQEE584 pKa = 3.79 EE585 pKa = 4.03 EE586 pKa = 4.12 RR587 pKa = 11.84 EE588 pKa = 4.04 RR589 pKa = 11.84 EE590 pKa = 3.87 EE591 pKa = 4.68 EE592 pKa = 4.09 EE593 pKa = 4.79 FGNTQQFMSEE603 pKa = 4.1 TDD605 pKa = 3.25 RR606 pKa = 11.84 QRR608 pKa = 11.84 AGPASSNDD616 pKa = 3.0 FATASQEE623 pKa = 3.87 MGQAQQGSHH632 pKa = 5.34 TQPEE636 pKa = 4.9 INTGTQLSQSTHH648 pKa = 4.97 TADD651 pKa = 3.81 PEE653 pKa = 4.43 EE654 pKa = 4.83 GTSAPSQAKK663 pKa = 8.99 RR664 pKa = 11.84 RR665 pKa = 11.84 RR666 pKa = 11.84 RR667 pKa = 11.84 EE668 pKa = 3.89 PEE670 pKa = 3.74 SLQEE674 pKa = 4.27 LLLIDD679 pKa = 4.13 SDD681 pKa = 4.26 GMCILIIFSCSYY693 pKa = 10.98 LILFII698 pKa = 5.4
Molecular weight: 79.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.565
IPC2_protein 5.563
IPC_protein 5.588
Toseland 5.906
ProMoST 5.893
Dawson 5.804
Bjellqvist 5.83
Wikipedia 5.779
Rodwell 5.779
Grimsley 6.033
Solomon 5.804
Lehninger 5.779
Nozaki 6.046
DTASelect 6.224
Thurlkill 6.198
EMBOSS 6.16
Sillero 6.148
Patrickios 3.999
IPC_peptide 5.817
IPC2_peptide 6.148
IPC2.peptide.svr19 6.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
698
698
698
698.0
79.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.45 ± 0.0
2.436 ± 0.0
4.728 ± 0.0
8.166 ± 0.0
3.725 ± 0.0
5.731 ± 0.0
3.009 ± 0.0
4.871 ± 0.0
5.301 ± 0.0
7.593 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.438 ± 0.0
3.725 ± 0.0
3.725 ± 0.0
7.307 ± 0.0
5.444 ± 0.0
5.731 ± 0.0
6.877 ± 0.0
6.59 ± 0.0
0.716 ± 0.0
3.438 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here