Mycobacterium phage Refuge

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482JBR9|A0A482JBR9_9CAUD Head-to-tail adaptor OS=Mycobacterium phage Refuge OX=2517967 GN=21 PE=4 SV=1
MM1 pKa = 6.79ITSYY5 pKa = 11.39DD6 pKa = 3.77DD7 pKa = 4.23SNGPTQAVIHH17 pKa = 6.39YY18 pKa = 9.24DD19 pKa = 3.66YY20 pKa = 11.05EE21 pKa = 5.18IDD23 pKa = 3.77PDD25 pKa = 4.19FSKK28 pKa = 10.93PLIPVDD34 pKa = 2.79KK35 pKa = 9.69WLVRR39 pKa = 11.84FGDD42 pKa = 4.06DD43 pKa = 5.26DD44 pKa = 4.18GLLDD48 pKa = 3.84VEE50 pKa = 4.75TFGTLLAHH58 pKa = 6.66KK59 pKa = 9.92VGVSLVQLSAVGTTVVEE76 pKa = 4.05PALNSIYY83 pKa = 10.43TYY85 pKa = 11.52

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482JB78|A0A482JB78_9CAUD Uncharacterized protein OS=Mycobacterium phage Refuge OX=2517967 GN=69 PE=4 SV=1
MM1 pKa = 7.24ATRR4 pKa = 11.84RR5 pKa = 11.84PPARR9 pKa = 11.84PRR11 pKa = 11.84AQKK14 pKa = 10.47QCVDD18 pKa = 3.74CAAAGITSKK27 pKa = 10.74RR28 pKa = 11.84KK29 pKa = 7.48TPHH32 pKa = 6.45PGPRR36 pKa = 11.84CATHH40 pKa = 6.32HH41 pKa = 5.47RR42 pKa = 11.84AKK44 pKa = 10.51RR45 pKa = 11.84RR46 pKa = 11.84VRR48 pKa = 11.84SSGAWGARR56 pKa = 11.84ILATYY61 pKa = 9.33GISPEE66 pKa = 4.42EE67 pKa = 3.69YY68 pKa = 7.39WQIYY72 pKa = 7.32EE73 pKa = 4.2FQGGRR78 pKa = 11.84CYY80 pKa = 10.34ICQRR84 pKa = 11.84ANGKK88 pKa = 8.03VKK90 pKa = 10.47RR91 pKa = 11.84LSVDD95 pKa = 3.16HH96 pKa = 6.58DD97 pKa = 3.92HH98 pKa = 6.59KK99 pKa = 9.96TGIVRR104 pKa = 11.84GLLCTMCNKK113 pKa = 8.53YY114 pKa = 8.37TLGWARR120 pKa = 11.84DD121 pKa = 3.61AIEE124 pKa = 4.13FFEE127 pKa = 4.95RR128 pKa = 11.84AIAYY132 pKa = 7.8LKK134 pKa = 10.4RR135 pKa = 11.84PPAVEE140 pKa = 4.23VIGEE144 pKa = 4.52RR145 pKa = 11.84IAPVEE150 pKa = 3.76ADD152 pKa = 3.95KK153 pKa = 11.39LRR155 pKa = 11.84SLPP158 pKa = 3.75

Molecular weight: 17.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

16839

27

1036

185.0

20.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.024 ± 0.389

0.814 ± 0.119

6.152 ± 0.214

6.313 ± 0.363

3.492 ± 0.136

8.552 ± 0.545

1.918 ± 0.175

5.018 ± 0.149

4.43 ± 0.198

7.934 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.158

3.266 ± 0.153

5.535 ± 0.232

3.747 ± 0.21

6.372 ± 0.352

5.885 ± 0.276

6.087 ± 0.189

7.168 ± 0.238

1.954 ± 0.134

2.756 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski