Naumovozyma dairenensis (strain ATCC 10597 / BCRC 20456 / CBS 421 / NBRC 0211 / NRRL Y-12639) (Saccharomyces dairenensis)
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5523 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G0WG71|G0WG71_NAUDC Uncharacterized protein OS=Naumovozyma dairenensis (strain ATCC 10597 / BCRC 20456 / CBS 421 / NBRC 0211 / NRRL Y-12639) OX=1071378 GN=NDAI0I02130 PE=4 SV=1
MM1 pKa = 7.6 GLFDD5 pKa = 3.91 QVKK8 pKa = 10.14 KK9 pKa = 10.33 FASGNNNNNDD19 pKa = 3.5 NNNNGNDD26 pKa = 3.4 NSNNNNDD33 pKa = 3.71 NSNSNNDD40 pKa = 3.59 DD41 pKa = 3.61 FVTKK45 pKa = 10.7 AEE47 pKa = 4.29 NYY49 pKa = 9.25 VGQDD53 pKa = 3.38 KK54 pKa = 11.03 VDD56 pKa = 3.41 QFKK59 pKa = 11.63 NKK61 pKa = 9.73 IGEE64 pKa = 4.33 EE65 pKa = 3.96 NFNTLEE71 pKa = 4.29 SKK73 pKa = 10.58 VRR75 pKa = 11.84 EE76 pKa = 4.21 QFSKK80 pKa = 10.18 TSLNDD85 pKa = 3.28 KK86 pKa = 9.87 NDD88 pKa = 3.51 SYY90 pKa = 11.97 GSSNNNGSYY99 pKa = 11.26 GNSNDD104 pKa = 3.7 NNNNNDD110 pKa = 3.5 AYY112 pKa = 10.66 GSSNNNSYY120 pKa = 11.68 GNDD123 pKa = 3.29 NSDD126 pKa = 4.49 SYY128 pKa = 11.87 GSSNNTDD135 pKa = 3.94 AYY137 pKa = 10.9 DD138 pKa = 3.76 SSNKK142 pKa = 9.03 QSSYY146 pKa = 11.84 GNDD149 pKa = 3.14 NDD151 pKa = 5.14 DD152 pKa = 5.15 SYY154 pKa = 12.24 GSSNKK159 pKa = 9.44 KK160 pKa = 10.04 SSYY163 pKa = 11.46 GNDD166 pKa = 3.11 NDD168 pKa = 5.14 DD169 pKa = 5.15 SYY171 pKa = 12.21 GSSNKK176 pKa = 9.36 QSSYY180 pKa = 11.84 GNDD183 pKa = 3.14 NDD185 pKa = 5.14 DD186 pKa = 5.15 SYY188 pKa = 12.21 GSSNKK193 pKa = 9.35 QSSYY197 pKa = 11.69 GDD199 pKa = 4.51 DD200 pKa = 3.76 NDD202 pKa = 5.03 DD203 pKa = 4.95 SYY205 pKa = 12.29 GSSNKK210 pKa = 9.42 KK211 pKa = 10.06 SSYY214 pKa = 11.1 GDD216 pKa = 3.52 DD217 pKa = 3.87 SYY219 pKa = 12.18 GSSNKK224 pKa = 9.36 QSSYY228 pKa = 11.84 GNDD231 pKa = 3.14 NDD233 pKa = 5.14 DD234 pKa = 5.15 SYY236 pKa = 12.24 GSSNKK241 pKa = 9.44 KK242 pKa = 10.04 SSYY245 pKa = 11.46 GNDD248 pKa = 3.11 NDD250 pKa = 4.35 DD251 pKa = 4.85 SYY253 pKa = 12.13 GSSHH257 pKa = 6.27 KK258 pKa = 10.3 QSSYY262 pKa = 11.88 GNDD265 pKa = 3.14 NDD267 pKa = 5.14 DD268 pKa = 5.15 SYY270 pKa = 12.24 GSSNKK275 pKa = 9.42 KK276 pKa = 10.06 SSYY279 pKa = 11.1 GDD281 pKa = 3.52 DD282 pKa = 3.87 SYY284 pKa = 12.18 GSSNKK289 pKa = 9.36 QSSYY293 pKa = 11.84 GNDD296 pKa = 3.14 NDD298 pKa = 5.14 DD299 pKa = 5.15 SYY301 pKa = 12.24 GSSNKK306 pKa = 9.44 KK307 pKa = 10.02 SSYY310 pKa = 11.37 GNDD313 pKa = 3.54 DD314 pKa = 3.79 DD315 pKa = 6.48 DD316 pKa = 6.85 SYY318 pKa = 12.17 GSSNKK323 pKa = 9.36 QSSYY327 pKa = 11.84 GNDD330 pKa = 3.14 NDD332 pKa = 5.14 DD333 pKa = 5.15 SYY335 pKa = 12.24 GSSNKK340 pKa = 9.44 KK341 pKa = 10.04 SSYY344 pKa = 11.46 GNDD347 pKa = 3.11 NDD349 pKa = 5.14 DD350 pKa = 5.15 SYY352 pKa = 12.21 GSSNKK357 pKa = 9.35 QSSYY361 pKa = 11.69 GDD363 pKa = 4.51 DD364 pKa = 3.76 NDD366 pKa = 5.03 DD367 pKa = 4.43 SYY369 pKa = 12.28 GSSNSNTYY377 pKa = 10.61 GNNNDD382 pKa = 4.21 DD383 pKa = 4.53 SYY385 pKa = 11.42 GTDD388 pKa = 3.05 RR389 pKa = 11.84 NSYY392 pKa = 10.45 GNSNQNSYY400 pKa = 11.9 SNDD403 pKa = 3.2 NNDD406 pKa = 2.72 SYY408 pKa = 12.16 GRR410 pKa = 11.84 GNGNSGNNSSYY421 pKa = 11.56 NNDD424 pKa = 3.3 YY425 pKa = 11.49
Molecular weight: 45.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.783
IPC_protein 3.859
Toseland 3.592
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.872
Rodwell 3.668
Grimsley 3.503
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.355
Thurlkill 3.668
EMBOSS 3.872
Sillero 3.986
Patrickios 0.579
IPC_peptide 3.872
IPC2_peptide 3.961
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|G0W3U2|G0W3U2_NAUDC Tr-type G domain-containing protein OS=Naumovozyma dairenensis (strain ATCC 10597 / BCRC 20456 / CBS 421 / NBRC 0211 / NRRL Y-12639) OX=1071378 GN=NDAI0A03230 PE=4 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.11 WRR6 pKa = 11.84 KK7 pKa = 9.07 KK8 pKa = 6.93 RR9 pKa = 11.84 TRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 9.48 RR15 pKa = 11.84 KK16 pKa = 8.41 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 9.19 VRR21 pKa = 11.84 ARR23 pKa = 11.84 SKK25 pKa = 10.98
Molecular weight: 3.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5523
0
5523
2865626
25
4961
518.9
58.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.804 ± 0.028
1.13 ± 0.011
6.001 ± 0.023
6.562 ± 0.031
4.309 ± 0.021
4.651 ± 0.028
2.123 ± 0.012
7.229 ± 0.021
7.49 ± 0.032
9.421 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.09 ± 0.01
7.178 ± 0.047
4.313 ± 0.022
4.051 ± 0.028
4.119 ± 0.021
8.845 ± 0.036
6.326 ± 0.028
5.05 ± 0.023
0.965 ± 0.009
3.338 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here