Staphylococcus phage phiIPLA-C1C
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 203 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3MVS9|A0A0D3MVS9_9CAUD NTP pyrophosphohydrolase OS=Staphylococcus phage phiIPLA-C1C OX=1572704 PE=4 SV=1
MM1 pKa = 7.39 TIKK4 pKa = 10.5 EE5 pKa = 3.88 FLQRR9 pKa = 11.84 QEE11 pKa = 3.75 EE12 pKa = 4.65 STVKK16 pKa = 10.56 DD17 pKa = 3.4 VANYY21 pKa = 9.14 GVQSGAIGEE30 pKa = 4.67 LIYY33 pKa = 10.93 TKK35 pKa = 10.71 DD36 pKa = 3.28 VVKK39 pKa = 10.75 FFDD42 pKa = 4.19 NYY44 pKa = 10.21 RR45 pKa = 11.84 QDD47 pKa = 3.1 IEE49 pKa = 4.72 TVVIDD54 pKa = 3.69 YY55 pKa = 10.74 LEE57 pKa = 4.32 SVTGVKK63 pKa = 10.15 YY64 pKa = 10.78 FNLLNYY70 pKa = 10.36 EE71 pKa = 4.17 LMNDD75 pKa = 3.86 LEE77 pKa = 4.62 NYY79 pKa = 10.06 VNVEE83 pKa = 4.08 FQHH86 pKa = 6.36 EE87 pKa = 4.04 DD88 pKa = 3.15 TYY90 pKa = 11.48 IEE92 pKa = 4.02 MEE94 pKa = 4.07 YY95 pKa = 10.87 QKK97 pKa = 11.07 AEE99 pKa = 4.18 EE100 pKa = 3.89 IARR103 pKa = 11.84 RR104 pKa = 11.84 DD105 pKa = 3.31 IEE107 pKa = 4.24 NFEE110 pKa = 5.34 DD111 pKa = 3.66 MTEE114 pKa = 4.13 DD115 pKa = 3.7 EE116 pKa = 4.18 QDD118 pKa = 3.39 EE119 pKa = 5.27 AITEE123 pKa = 4.09 CLDD126 pKa = 4.11 LVEE129 pKa = 6.3 LDD131 pKa = 4.54 LQDD134 pKa = 3.32 TDD136 pKa = 3.94 KK137 pKa = 11.62 VQFVNLAVEE146 pKa = 4.99 IVAQDD151 pKa = 3.46 MTEE154 pKa = 3.87 EE155 pKa = 4.36 IYY157 pKa = 11.13
Molecular weight: 18.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.916
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.617
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.656
Rodwell 3.63
Grimsley 3.528
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.668
Sillero 3.91
Patrickios 2.613
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.838
Protein with the highest isoelectric point:
>tr|A0A0D3MV55|A0A0D3MV55_9CAUD Tail protein OS=Staphylococcus phage phiIPLA-C1C OX=1572704 PE=4 SV=1
MM1 pKa = 7.65 TNRR4 pKa = 11.84 KK5 pKa = 9.77 SIGKK9 pKa = 8.35 MSKK12 pKa = 8.33 TRR14 pKa = 11.84 ATWHH18 pKa = 6.11 IKK20 pKa = 10.2 PYY22 pKa = 9.06 TQVKK26 pKa = 9.45 KK27 pKa = 10.87 DD28 pKa = 3.22 KK29 pKa = 10.4 TKK31 pKa = 8.33 YY32 pKa = 6.98 TRR34 pKa = 11.84 KK35 pKa = 9.59 RR36 pKa = 11.84 KK37 pKa = 8.97 HH38 pKa = 6.33 KK39 pKa = 10.71 KK40 pKa = 9.36 RR41 pKa = 11.84 DD42 pKa = 3.47 DD43 pKa = 3.65 YY44 pKa = 12.12
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.241
IPC2_protein 10.131
IPC_protein 10.599
Toseland 11.052
ProMoST 10.613
Dawson 11.125
Bjellqvist 10.745
Wikipedia 11.272
Rodwell 11.667
Grimsley 11.155
Solomon 11.199
Lehninger 11.184
Nozaki 11.008
DTASelect 10.745
Thurlkill 11.023
EMBOSS 11.433
Sillero 11.038
Patrickios 11.403
IPC_peptide 11.213
IPC2_peptide 9.326
IPC2.peptide.svr19 8.283
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
203
0
203
40989
43
1151
201.9
23.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.655 ± 0.204
0.581 ± 0.047
6.814 ± 0.138
8.222 ± 0.248
4.05 ± 0.137
5.321 ± 0.299
1.583 ± 0.083
7.841 ± 0.208
10.086 ± 0.235
8.173 ± 0.181
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.174 ± 0.096
7.387 ± 0.192
2.525 ± 0.118
3.289 ± 0.191
3.625 ± 0.112
6.392 ± 0.166
5.921 ± 0.17
6.241 ± 0.165
0.803 ± 0.066
5.319 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here