Bosea sp. RAC05

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5349 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3NCA9|A0A1B3NCA9_9BRAD Meiotically up-regulated protein OS=Bosea sp. RAC05 OX=1842539 GN=BSY19_4984 PE=4 SV=1
MM1 pKa = 7.68SSTPYY6 pKa = 10.37YY7 pKa = 10.51DD8 pKa = 3.81GVVIDD13 pKa = 3.8QTDD16 pKa = 3.11RR17 pKa = 11.84SAPLEE22 pKa = 4.02IEE24 pKa = 4.7FGRR27 pKa = 11.84SSDD30 pKa = 3.38WQDD33 pKa = 2.79EE34 pKa = 4.13NLIYY38 pKa = 10.65LVVDD42 pKa = 3.87GRR44 pKa = 11.84PLIMDD49 pKa = 5.09DD50 pKa = 2.97ATGRR54 pKa = 11.84QIYY57 pKa = 7.82TAMMKK62 pKa = 10.43LGAYY66 pKa = 9.89LGYY69 pKa = 10.66DD70 pKa = 3.28QAPP73 pKa = 3.35

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3NPW2|A0A1B3NPW2_9BRAD Uncharacterized protein OS=Bosea sp. RAC05 OX=1842539 GN=BSY19_879 PE=4 SV=1
MM1 pKa = 7.02TPKK4 pKa = 9.88IRR6 pKa = 11.84PAGSLSVAIKK16 pKa = 10.6LVMLAMVVPRR26 pKa = 11.84VLRR29 pKa = 11.84FKK31 pKa = 10.92LRR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 9.15PP36 pKa = 3.41

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5349

0

5349

1676541

29

2260

313.4

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.686 ± 0.052

0.815 ± 0.011

5.404 ± 0.025

5.492 ± 0.03

3.594 ± 0.022

8.713 ± 0.028

1.914 ± 0.017

5.059 ± 0.024

3.057 ± 0.03

10.4 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.015

2.179 ± 0.017

5.554 ± 0.031

3.079 ± 0.018

7.47 ± 0.033

5.245 ± 0.028

5.222 ± 0.025

7.447 ± 0.027

1.299 ± 0.013

1.96 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski