Beihai hepe-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJN7|A0A1L3KJN7_9VIRU Uncharacterized protein OS=Beihai hepe-like virus 9 OX=1922386 PE=4 SV=1
MM1 pKa = 7.41YY2 pKa = 10.88SLILLFLAGASAQTSVEE19 pKa = 4.11LIGWQFEE26 pKa = 4.25EE27 pKa = 5.09LIKK30 pKa = 10.95ASSSSNTPKK39 pKa = 10.3VVPFDD44 pKa = 4.38CNNFPNNAPYY54 pKa = 9.87WSCSDD59 pKa = 3.4TYY61 pKa = 10.59RR62 pKa = 11.84TVTLNLLIPKK72 pKa = 9.57GAYY75 pKa = 9.66LPSFVPGPTPSTDD88 pKa = 3.09DD89 pKa = 2.88MGYY92 pKa = 10.5CVGTSTIGPFPMNNMLAVMKK112 pKa = 10.21QLATIIIHH120 pKa = 5.69YY121 pKa = 8.92KK122 pKa = 8.58SHH124 pKa = 7.34FIVEE128 pKa = 4.5LQTPDD133 pKa = 4.11NYY135 pKa = 11.29DD136 pKa = 3.64FYY138 pKa = 11.28NGSPLVVAHH147 pKa = 5.36YY148 pKa = 8.94TGKK151 pKa = 9.73PLDD154 pKa = 3.64VTVSLKK160 pKa = 10.74EE161 pKa = 3.96VDD163 pKa = 3.49NMLYY167 pKa = 10.66ALSQLAFKK175 pKa = 9.01QTQFHH180 pKa = 6.04VDD182 pKa = 3.32EE183 pKa = 5.09HH184 pKa = 5.34VTAIDD189 pKa = 3.66LTGLALATGSKK200 pKa = 9.93AYY202 pKa = 10.58SSGTSVPSIIKK213 pKa = 9.27FFNYY217 pKa = 9.81TYY219 pKa = 11.11VSPFDD224 pKa = 3.99KK225 pKa = 10.79NITIISPDD233 pKa = 3.12YY234 pKa = 8.69YY235 pKa = 9.72YY236 pKa = 10.85TVFFYY241 pKa = 10.83DD242 pKa = 4.39PSAQNSISAITYY254 pKa = 7.19TNPGLQLVTEE264 pKa = 4.55LVEE267 pKa = 3.94EE268 pKa = 4.49TEE270 pKa = 3.9ISKK273 pKa = 10.71NIQFRR278 pKa = 11.84PGITVAHH285 pKa = 6.0RR286 pKa = 11.84QLMKK290 pKa = 10.61NVPIANYY297 pKa = 9.76GKK299 pKa = 10.22RR300 pKa = 11.84LTPIISFFHH309 pKa = 6.24SEE311 pKa = 3.94NYY313 pKa = 10.23LLSSWLRR320 pKa = 11.84FQNYY324 pKa = 6.34YY325 pKa = 8.71TYY327 pKa = 10.83KK328 pKa = 10.42GLNITSDD335 pKa = 3.65QLSQSIPDD343 pKa = 3.37SMRR346 pKa = 11.84NLVYY350 pKa = 9.56VQCNEE355 pKa = 3.94RR356 pKa = 11.84PDD358 pKa = 3.44MCSCKK363 pKa = 10.5GNYY366 pKa = 9.33SSQAMAIGALGYY378 pKa = 10.79DD379 pKa = 3.74SIYY382 pKa = 8.86TKK384 pKa = 10.89RR385 pKa = 11.84MNTVIYY391 pKa = 9.58PGEE394 pKa = 4.2KK395 pKa = 9.62FHH397 pKa = 5.93VHH399 pKa = 5.53SPIMSHH405 pKa = 6.05VGSVPKK411 pKa = 10.21QGRR414 pKa = 11.84YY415 pKa = 7.2QFMDD419 pKa = 3.25TTLPNLPDD427 pKa = 4.64FKK429 pKa = 11.06AVNIPSWLPEE439 pKa = 4.04SLGEE443 pKa = 4.22EE444 pKa = 4.05INGVLDD450 pKa = 3.89AVQNIYY456 pKa = 9.09EE457 pKa = 4.58HH458 pKa = 6.86IQTFWEE464 pKa = 4.05ILQHH468 pKa = 7.12IEE470 pKa = 4.23EE471 pKa = 5.51IITAIQDD478 pKa = 3.31ITSLKK483 pKa = 7.97PTGGAPPEE491 pKa = 4.23AVPVV495 pKa = 3.65

Molecular weight: 55.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJP0|A0A1L3KJP0_9VIRU Uncharacterized protein OS=Beihai hepe-like virus 9 OX=1922386 PE=4 SV=1
MM1 pKa = 6.65THH3 pKa = 6.72ILAFRR8 pKa = 11.84VSNFVFQVEE17 pKa = 4.35FDD19 pKa = 3.86YY20 pKa = 11.61DD21 pKa = 3.97DD22 pKa = 4.78APLEE26 pKa = 4.06ARR28 pKa = 11.84KK29 pKa = 9.36QLISLGNPKK38 pKa = 10.09GRR40 pKa = 11.84GIPEE44 pKa = 4.23PLHH47 pKa = 6.01VVEE50 pKa = 4.58YY51 pKa = 11.15SRR53 pKa = 11.84FEE55 pKa = 3.86GGKK58 pKa = 9.99VIEE61 pKa = 4.36VEE63 pKa = 3.93PNYY66 pKa = 10.22ILYY69 pKa = 10.05HH70 pKa = 5.78KK71 pKa = 10.54QPLHH75 pKa = 6.25PFFVSKK81 pKa = 10.24FRR83 pKa = 11.84NRR85 pKa = 11.84LNFVRR90 pKa = 11.84VISNCSQQSHH100 pKa = 5.92IPEE103 pKa = 3.98SFYY106 pKa = 11.19TNFISNWQMQQ116 pKa = 3.28

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3302

116

2150

660.4

74.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.389 ± 0.873

1.575 ± 0.26

5.451 ± 0.665

5.996 ± 0.712

4.21 ± 0.643

5.996 ± 0.286

3.331 ± 0.423

6.299 ± 0.714

5.451 ± 0.488

7.117 ± 0.522

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.24

4.815 ± 0.435

5.27 ± 0.601

4.573 ± 0.203

3.786 ± 0.609

6.602 ± 1.024

7.268 ± 0.429

6.784 ± 0.288

1.211 ± 0.083

4.603 ± 0.537
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski