Sanxia tombus-like virus 9
Average proteome isoelectric point is 8.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGB8|A0A1L3KGB8_9VIRU Uncharacterized protein OS=Sanxia tombus-like virus 9 OX=1923393 PE=4 SV=1
MM1 pKa = 7.79 LSRR4 pKa = 11.84 LHH6 pKa = 6.34 VCFHH10 pKa = 6.05 HH11 pKa = 7.42 RR12 pKa = 11.84 GRR14 pKa = 11.84 QLLPPLCSLVPMVPAAHH31 pKa = 6.54 VDD33 pKa = 3.63 NVVSSIEE40 pKa = 3.78 LRR42 pKa = 11.84 VARR45 pKa = 11.84 EE46 pKa = 3.86 VPRR49 pKa = 11.84 PHH51 pKa = 7.32 PLVCAPKK58 pKa = 10.45 LRR60 pKa = 11.84 GKK62 pKa = 9.92 LRR64 pKa = 11.84 PYY66 pKa = 10.19 LLHH69 pKa = 6.95 PAPLVPTPLAMPLSPPRR86 pKa = 11.84 KK87 pKa = 8.44 VPPRR91 pKa = 11.84 KK92 pKa = 8.64 PQHH95 pKa = 6.53 LSSQLWEE102 pKa = 4.21 KK103 pKa = 10.29 VQAAVNPEE111 pKa = 4.06 LVLATTANATDD122 pKa = 3.63 TSLPTAAADD131 pKa = 3.58 HH132 pKa = 6.59 RR133 pKa = 11.84 LDD135 pKa = 4.02 PSSQAVHH142 pKa = 6.86 PCRR145 pKa = 11.84 NPEE148 pKa = 3.91 NCSLLLFPGVPKK160 pKa = 10.42 SDD162 pKa = 3.27 PHH164 pKa = 7.99 GPEE167 pKa = 3.79 SLAMVQKK174 pKa = 11.03 AMGMVPEE181 pKa = 4.54 SRR183 pKa = 11.84 TEE185 pKa = 3.9 NLQQLADD192 pKa = 4.09 EE193 pKa = 4.5 YY194 pKa = 10.79 RR195 pKa = 11.84 ANHH198 pKa = 6.83 LLPVLGRR205 pKa = 11.84 VHH207 pKa = 7.08 PLPWEE212 pKa = 3.74 EE213 pKa = 3.51 WLQRR217 pKa = 11.84 FPSNRR222 pKa = 11.84 SLQLNDD228 pKa = 3.15 ARR230 pKa = 11.84 SNVQLRR236 pKa = 11.84 GLLKK240 pKa = 10.3 SDD242 pKa = 4.03 CKK244 pKa = 10.89 VDD246 pKa = 3.69 CFLKK250 pKa = 11.06 VEE252 pKa = 4.36 TSTNATDD259 pKa = 3.86 PRR261 pKa = 11.84 NISPRR266 pKa = 11.84 SAEE269 pKa = 3.98 FLSVLGPYY277 pKa = 9.49 VAGLEE282 pKa = 4.16 NKK284 pKa = 9.3 IRR286 pKa = 11.84 TLHH289 pKa = 6.66 DD290 pKa = 3.75 HH291 pKa = 6.95 DD292 pKa = 4.65 QIPYY296 pKa = 9.23 LVKK299 pKa = 10.64 GLTPSARR306 pKa = 11.84 AQIVRR311 pKa = 11.84 RR312 pKa = 11.84 KK313 pKa = 7.88 EE314 pKa = 3.83 RR315 pKa = 11.84 AWVVEE320 pKa = 3.85 IDD322 pKa = 3.43 YY323 pKa = 11.06 SRR325 pKa = 11.84 YY326 pKa = 11.07 DD327 pKa = 3.31 MTISEE332 pKa = 4.2 DD333 pKa = 3.62 VIRR336 pKa = 11.84 EE337 pKa = 4.03 LEE339 pKa = 4.04 IKK341 pKa = 10.16 NFKK344 pKa = 10.69 ALFVSDD350 pKa = 4.58 EE351 pKa = 4.4 DD352 pKa = 4.1 PALDD356 pKa = 3.79 AVLDD360 pKa = 4.23 ALISMNGYY368 pKa = 9.72 NSLGVKK374 pKa = 8.64 YY375 pKa = 8.77 TVDD378 pKa = 3.43 GTRR381 pKa = 11.84 ASGDD385 pKa = 2.95 AHH387 pKa = 5.71 TSIGNGLLGRR397 pKa = 11.84 FLLWVCLRR405 pKa = 11.84 GLPDD409 pKa = 3.75 TVWDD413 pKa = 4.21 SVHH416 pKa = 6.61 EE417 pKa = 4.52 GDD419 pKa = 5.97 DD420 pKa = 3.52 GLLFVDD426 pKa = 5.8 DD427 pKa = 4.61 GWQHH431 pKa = 6.84 LVMHH435 pKa = 7.05 CLQVLPCFGFQAKK448 pKa = 9.66 LRR450 pKa = 11.84 LCRR453 pKa = 11.84 LDD455 pKa = 4.93 DD456 pKa = 4.1 ALFCGRR462 pKa = 11.84 LNVNTSNGAIEE473 pKa = 4.24 IADD476 pKa = 3.69 IPRR479 pKa = 11.84 TLGKK483 pKa = 10.21 FHH485 pKa = 6.92 TSCKK489 pKa = 9.83 TGDD492 pKa = 3.54 ARR494 pKa = 11.84 TLALAKK500 pKa = 10.35 AFSYY504 pKa = 10.78 YY505 pKa = 9.38 HH506 pKa = 6.69 TDD508 pKa = 2.88 RR509 pKa = 11.84 DD510 pKa = 4.1 TPIIGPLCWALIQHH524 pKa = 6.64 LRR526 pKa = 11.84 PLVSPRR532 pKa = 11.84 AFTRR536 pKa = 11.84 RR537 pKa = 11.84 INTEE541 pKa = 2.94 KK542 pKa = 9.78 WFEE545 pKa = 4.04 RR546 pKa = 11.84 EE547 pKa = 3.88 KK548 pKa = 10.74 IEE550 pKa = 5.64 AGMKK554 pKa = 7.9 VQRR557 pKa = 11.84 PPRR560 pKa = 11.84 ITPEE564 pKa = 3.02 ARR566 pKa = 11.84 AAVDD570 pKa = 3.33 RR571 pKa = 11.84 VFDD574 pKa = 3.55 VGYY577 pKa = 10.67 GSQLVLEE584 pKa = 4.79 KK585 pKa = 10.64 QLSSWASGVSEE596 pKa = 4.56 VPPLILTGEE605 pKa = 4.32 EE606 pKa = 4.54 VVDD609 pKa = 3.57 HH610 pKa = 6.69 TDD612 pKa = 3.06 GVKK615 pKa = 9.55 TVTVYY620 pKa = 11.17 AA621 pKa = 4.5
Molecular weight: 69.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.925
IPC2_protein 7.19
IPC_protein 7.088
Toseland 6.927
ProMoST 7.673
Dawson 7.746
Bjellqvist 8.141
Wikipedia 7.658
Rodwell 7.761
Grimsley 7.029
Solomon 7.775
Lehninger 7.805
Nozaki 8.448
DTASelect 7.936
Thurlkill 7.98
EMBOSS 7.995
Sillero 8.287
Patrickios 4.507
IPC_peptide 7.775
IPC2_peptide 7.483
IPC2.peptide.svr19 7.197
Protein with the highest isoelectric point:
>tr|A0A1L3KGI7|A0A1L3KGI7_9VIRU Chropara_Vmeth domain-containing protein OS=Sanxia tombus-like virus 9 OX=1923393 PE=4 SV=1
MM1 pKa = 6.89 VAWLPVVIGFAITALMAPRR20 pKa = 11.84 IEE22 pKa = 4.58 EE23 pKa = 4.0 DD24 pKa = 2.98 LSYY27 pKa = 11.16 YY28 pKa = 10.55 EE29 pKa = 4.85 SLGAPGWRR37 pKa = 11.84 TVDD40 pKa = 3.1 AYY42 pKa = 11.47 LPILKK47 pKa = 10.23 YY48 pKa = 10.68 LDD50 pKa = 4.27 LVPSPEE56 pKa = 3.79 RR57 pKa = 11.84 LEE59 pKa = 4.43 RR60 pKa = 11.84 LRR62 pKa = 11.84 LDD64 pKa = 3.48 CAIIQGEE71 pKa = 4.0 IHH73 pKa = 5.9 EE74 pKa = 4.48 WEE76 pKa = 3.5 WWAEE80 pKa = 3.95 YY81 pKa = 10.02 FPTMAGEE88 pKa = 4.5 RR89 pKa = 11.84 PALPYY94 pKa = 9.83 RR95 pKa = 11.84 CSYY98 pKa = 9.35 FVWNRR103 pKa = 11.84 TAKK106 pKa = 10.06 PAVTSRR112 pKa = 11.84 LDD114 pKa = 3.81 LLMSAYY120 pKa = 9.17 ATIAKK125 pKa = 9.98 GLLFLGAWVWINVALIVLAFICCLLAGVLLVVKK158 pKa = 10.29 VYY160 pKa = 8.38 LTYY163 pKa = 10.43 YY164 pKa = 9.3 RR165 pKa = 11.84 GVRR168 pKa = 11.84 VRR170 pKa = 11.84 APTPVLGKK178 pKa = 10.52 NEE180 pKa = 3.94 VRR182 pKa = 11.84 DD183 pKa = 3.81 LLAVEE188 pKa = 4.83 LSKK191 pKa = 10.16 TPIDD195 pKa = 4.74 KK196 pKa = 10.53 IQLLADD202 pKa = 4.35 GKK204 pKa = 9.91 GHH206 pKa = 6.87 PVLAKK211 pKa = 9.84 TRR213 pKa = 11.84 ATLEE217 pKa = 4.23 TACQQVLLRR226 pKa = 11.84 MNNKK230 pKa = 8.94 IRR232 pKa = 11.84 DD233 pKa = 3.72 VGGSVSRR240 pKa = 11.84 NQKK243 pKa = 9.62 LGKK246 pKa = 9.34 SLHH249 pKa = 5.63 VCFPVLTAADD259 pKa = 4.0 RR260 pKa = 11.84 ARR262 pKa = 11.84 VNRR265 pKa = 11.84 ALANDD270 pKa = 3.52 VGFHH274 pKa = 6.89 LGHH277 pKa = 6.37 EE278 pKa = 4.75 CDD280 pKa = 3.67 VKK282 pKa = 11.01 HH283 pKa = 6.47 IPTYY287 pKa = 9.77 MSYY290 pKa = 10.86 TDD292 pKa = 3.64 FHH294 pKa = 6.94 MPLDD298 pKa = 3.65 MLVRR302 pKa = 11.84 TVTSPTLIITHH313 pKa = 6.78 DD314 pKa = 4.07 FRR316 pKa = 11.84 NMPAKK321 pKa = 10.62 LFDD324 pKa = 3.89 EE325 pKa = 4.65 EE326 pKa = 4.37 ATISVIGGLVNMTTRR341 pKa = 11.84 GGSKK345 pKa = 8.78 YY346 pKa = 7.0 THH348 pKa = 7.27 GYY350 pKa = 9.56 HH351 pKa = 6.34 SWDD354 pKa = 3.77 DD355 pKa = 3.64 EE356 pKa = 4.78 GSVVTKK362 pKa = 10.65 SGAFYY367 pKa = 8.01 YY368 pKa = 10.63 HH369 pKa = 6.96 KK370 pKa = 10.54 VAEE373 pKa = 4.77 LGDD376 pKa = 4.31 SIVIYY381 pKa = 8.07 ATPLNGDD388 pKa = 3.87 HH389 pKa = 7.84 DD390 pKa = 4.14 PTQDD394 pKa = 3.5 NEE396 pKa = 4.28 LQSSVNCYY404 pKa = 9.59 TGIKK408 pKa = 9.08 YY409 pKa = 10.39 RR410 pKa = 11.84 GGRR413 pKa = 11.84 AEE415 pKa = 4.1 LHH417 pKa = 5.46 NGEE420 pKa = 4.66 YY421 pKa = 10.3 IMRR424 pKa = 11.84 DD425 pKa = 3.23 DD426 pKa = 4.03 QGNLRR431 pKa = 11.84 GAVSARR437 pKa = 11.84 TVSRR441 pKa = 11.84 VAHH444 pKa = 5.42 SFGYY448 pKa = 10.46 AKK450 pKa = 10.12 RR451 pKa = 11.84 DD452 pKa = 3.47 AKK454 pKa = 11.08 FEE456 pKa = 4.15 TGLAANLRR464 pKa = 11.84 ARR466 pKa = 11.84 FTQDD470 pKa = 3.69 GEE472 pKa = 4.36 DD473 pKa = 3.49 HH474 pKa = 6.74 SLLPEE479 pKa = 4.18 ATNLTVALADD489 pKa = 5.4 RR490 pKa = 11.84 IALAADD496 pKa = 4.25 MSAVSCPTEE505 pKa = 3.64 MNFFQRR511 pKa = 11.84 VMWNAYY517 pKa = 7.91 FALVSVLPTSLQLARR532 pKa = 11.84 TVRR535 pKa = 11.84 QMLLQSEE542 pKa = 4.6 HH543 pKa = 4.24 VRR545 pKa = 11.84 FRR547 pKa = 11.84 KK548 pKa = 9.1 WMWEE552 pKa = 3.87 EE553 pKa = 3.59 VHH555 pKa = 6.46 IPNYY559 pKa = 10.21 LVSTPGLVSLRR570 pKa = 11.84 ADD572 pKa = 3.11 AVKK575 pKa = 10.53 ASRR578 pKa = 11.84 LFPSPGAATAAPAVLACTNGAGCACGQCGQQHH610 pKa = 5.67 RR611 pKa = 11.84 TQGSSGSPAAASLSLRR627 pKa = 11.84 PQTTRR632 pKa = 11.84 QTTSLSPTPSPTSTNTTSNAPVPAKK657 pKa = 10.46 KK658 pKa = 9.1 GTASQAPAPQQPAVGKK674 pKa = 9.91 GAGSGKK680 pKa = 10.33 SRR682 pKa = 11.84 IGPCNYY688 pKa = 9.51 CKK690 pKa = 10.34 RR691 pKa = 11.84 HH692 pKa = 4.39 GHH694 pKa = 6.15 VIANCRR700 pKa = 11.84 SRR702 pKa = 11.84 PSAGSVKK709 pKa = 10.4 PGGSPVQKK717 pKa = 10.02 PGKK720 pKa = 8.46 LQPAAVPGGAQVRR733 pKa = 11.84 PSRR736 pKa = 11.84 AGKK739 pKa = 9.25 PSNGAKK745 pKa = 10.61 GNGNGTRR752 pKa = 11.84 KK753 pKa = 9.93 QNGKK757 pKa = 8.73 PAATRR762 pKa = 11.84 GRR764 pKa = 11.84 VQSKK768 pKa = 10.42 SPAPGARR775 pKa = 11.84 ARR777 pKa = 11.84 PPPPVGRR784 pKa = 11.84 VAAAVPVKK792 pKa = 10.25 PFTPAQRR799 pKa = 11.84 RR800 pKa = 11.84 AQQCATSRR808 pKa = 11.84 AVKK811 pKa = 10.08 II812 pKa = 4.02
Molecular weight: 87.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.939
IPC2_protein 9.107
IPC_protein 9.092
Toseland 9.677
ProMoST 9.487
Dawson 9.955
Bjellqvist 9.721
Wikipedia 10.145
Rodwell 10.218
Grimsley 10.043
Solomon 9.984
Lehninger 9.94
Nozaki 9.838
DTASelect 9.677
Thurlkill 9.809
EMBOSS 10.116
Sillero 9.911
Patrickios 5.232
IPC_peptide 9.984
IPC2_peptide 8.653
IPC2.peptide.svr19 8.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1787
354
812
595.7
64.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.289 ± 1.294
1.903 ± 0.246
4.98 ± 0.736
3.693 ± 0.59
2.63 ± 0.094
7.219 ± 0.854
2.462 ± 0.53
3.078 ± 0.32
4.757 ± 0.425
9.905 ± 1.187
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.847 ± 0.109
3.693 ± 0.196
7.499 ± 0.326
3.637 ± 0.192
6.771 ± 0.458
7.163 ± 0.504
6.715 ± 0.899
8.842 ± 0.549
1.679 ± 0.141
2.238 ± 0.476
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here